BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30101.Seq
(454 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g19390.1 68416.m02459 cysteine proteinase, putative / thiol p... 30 0.64
At5g67100.1 68418.m08460 DNA-directed DNA polymerase alpha catal... 29 1.9
At4g11900.1 68417.m01893 S-locus lectin protein kinase family pr... 28 2.6
At1g17590.3 68414.m02169 CCAAT-binding transcription factor (CBF... 28 2.6
At1g17590.2 68414.m02168 CCAAT-binding transcription factor (CBF... 28 2.6
At1g17590.1 68414.m02167 CCAAT-binding transcription factor (CBF... 28 2.6
At1g08140.1 68414.m00896 cation/hydrogen exchanger (CHX6a) Note:... 28 3.4
At3g06180.1 68416.m00710 expressed protein 27 4.5
At5g05110.1 68418.m00542 cysteine protease inhibitor, putative /... 27 7.8
>At3g19390.1 68416.m02459 cysteine proteinase, putative / thiol
protease, putative contains similarity to cysteine
proteinase RD21A (thiol protease) GI:435619, SP:P43297
from [Arabidopsis thaliana]
Length = 452
Score = 30.3 bits (65), Expect = 0.64
Identities = 12/31 (38%), Positives = 18/31 (58%)
Frame = +2
Query: 260 KHGGECFSKGYCSQSLIYEEASDCPEGXDCC 352
++ G+C+S G C YE A+ C +G CC
Sbjct: 380 EYNGKCYSWGCCP----YESATCCDDGSSCC 406
>At5g67100.1 68418.m08460 DNA-directed DNA polymerase alpha
catalytic subunit, putative similar to SP|O48653 DNA
polymerase alpha catalytic subunit (EC 2.7.7.7) {Oryza
sativa}; contains Pfam profiles: PF03175 DNA polymerase
type B, organellar and viral, PF00136 DNA polymerase
family B, PF03104 DNA polymerase family B, exonuclease
domain
Length = 1492
Score = 28.7 bits (61), Expect = 1.9
Identities = 16/71 (22%), Positives = 32/71 (45%), Gaps = 1/71 (1%)
Frame = -3
Query: 335 RDSLKLLHISKIDCSSLSRNILR-RASDSGLFYGHSVTAFDSVFLLLRTESSSGSDIRWT 159
++ +L I + + L+R L DS + GH+++ FD LL R ++ W+
Sbjct: 637 KNGCNVLSIENSERALLNRLFLELNKLDSDILVGHNISGFDLDVLLQRAQACKVQSSMWS 696
Query: 158 VLSHSTQTTFP 126
+ ++ P
Sbjct: 697 KIGRLKRSFMP 707
>At4g11900.1 68417.m01893 S-locus lectin protein kinase family
protein contains Pfam domains, PF00954: S-locus
glycoprotein family, PF00069: Protein kinase domain, and
PF01453: Lectin (probable mannose binding)
Length = 849
Score = 28.3 bits (60), Expect = 2.6
Identities = 13/43 (30%), Positives = 22/43 (51%)
Frame = -3
Query: 221 FDSVFLLLRTESSSGSDIRWTVLSHSTQTTFPAARILLFNGIY 93
FDS L+LR +S + + W H + T P +I L + ++
Sbjct: 160 FDSGNLVLRDGPNSSAAVLWQSFDHPSDTWLPGGKIRLGSQLF 202
>At1g17590.3 68414.m02169 CCAAT-binding transcription factor
(CBF-B/NF-YA) family protein contains Pfam profile:
PF02045 CCAAT-binding transcription factor (CBF-B/NF-YA)
subunit B
Length = 328
Score = 28.3 bits (60), Expect = 2.6
Identities = 13/31 (41%), Positives = 20/31 (64%)
Frame = -3
Query: 323 KLLHISKIDCSSLSRNILRRASDSGLFYGHS 231
+L + + DCS+ SR+ + ASDS +GHS
Sbjct: 261 QLQNSNDCDCSTTSRSDITSASDSVNLFGHS 291
>At1g17590.2 68414.m02168 CCAAT-binding transcription factor
(CBF-B/NF-YA) family protein contains Pfam profile:
PF02045 CCAAT-binding transcription factor (CBF-B/NF-YA)
subunit B
Length = 328
Score = 28.3 bits (60), Expect = 2.6
Identities = 13/31 (41%), Positives = 20/31 (64%)
Frame = -3
Query: 323 KLLHISKIDCSSLSRNILRRASDSGLFYGHS 231
+L + + DCS+ SR+ + ASDS +GHS
Sbjct: 261 QLQNSNDCDCSTTSRSDITSASDSVNLFGHS 291
>At1g17590.1 68414.m02167 CCAAT-binding transcription factor
(CBF-B/NF-YA) family protein contains Pfam profile:
PF02045 CCAAT-binding transcription factor (CBF-B/NF-YA)
subunit B
Length = 328
Score = 28.3 bits (60), Expect = 2.6
Identities = 13/31 (41%), Positives = 20/31 (64%)
Frame = -3
Query: 323 KLLHISKIDCSSLSRNILRRASDSGLFYGHS 231
+L + + DCS+ SR+ + ASDS +GHS
Sbjct: 261 QLQNSNDCDCSTTSRSDITSASDSVNLFGHS 291
>At1g08140.1 68414.m00896 cation/hydrogen exchanger (CHX6a) Note:
CHX6a and CHX6b were originally 1 gene but were split
pased on alignments with other family members; may be a
pseudogene and requires futher investigation; monovalent
cation:proton antiporter family 2 (CPA2) member,
PMID:11500563
Length = 818
Score = 27.9 bits (59), Expect = 3.4
Identities = 21/80 (26%), Positives = 34/80 (42%), Gaps = 8/80 (10%)
Frame = -3
Query: 296 CSSLSRNILRRASDSGLF--------YGHSVTAFDSVFLLLRTESSSGSDIRWTVLSHST 141
C SL+ R DS + + S++AF S+ LL+ S+ LS +
Sbjct: 169 CGSLTFRYRERRGDSSILRMEYRLIIFLQSISAFTSIDTLLKDLQIKHSEFGRIALSGAM 228
Query: 140 QTTFPAARILLFNGIYFTEI 81
T A + FN IY+ ++
Sbjct: 229 VTDMLAFGVTFFNAIYYEKL 248
>At3g06180.1 68416.m00710 expressed protein
Length = 241
Score = 27.5 bits (58), Expect = 4.5
Identities = 16/36 (44%), Positives = 18/36 (50%)
Frame = -1
Query: 181 LALISDGQSSATVHKPPSLRHGSSCSMESISQKSSS 74
L L+SD + S T PPS S S S S SSS
Sbjct: 4 LLLLSDEEHSTTNSMPPSSSASRSASNHSSSSSSSS 39
>At5g05110.1 68418.m00542 cysteine protease inhibitor, putative /
cystatin, putative similar to cysteine proteinase
inhibitor [Glycine max] GI:1944342; contains Pfam
profile PF00031: Cystatin domain
Length = 232
Score = 26.6 bits (56), Expect = 7.8
Identities = 10/23 (43%), Positives = 17/23 (73%)
Frame = -1
Query: 133 PSLRHGSSCSMESISQKSSSLFP 65
P ++ + +M+S+ QKS+SLFP
Sbjct: 162 PEVQEAAKHAMKSLQQKSNSLFP 184
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,467,492
Number of Sequences: 28952
Number of extensions: 175341
Number of successful extensions: 574
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 559
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 574
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 742437000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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