BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30100.Seq (586 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 65 6e-12 SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 51 1e-07 SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 49 6e-07 SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 41 1e-04 SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 40 4e-04 SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 36 0.003 SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 34 0.018 SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 29 0.50 SPBC4B4.11 |||conserved fungal protein|Schizosaccharomyces pombe... 29 0.50 SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 28 1.2 SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9... 27 1.5 SPCC24B10.15 |||PINc domain|Schizosaccharomyces pombe|chr 3|||Ma... 25 6.2 SPCC330.08 |alg11|gmd3|alpha-1,2-mannosyltransferase Alg11|Schiz... 25 8.1 SPCC306.03c |cnd2||condensin subunit Cnd2|Schizosaccharomyces po... 25 8.1 >SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 492 Score = 65.3 bits (152), Expect = 6e-12 Identities = 33/85 (38%), Positives = 51/85 (60%), Gaps = 2/85 (2%) Frame = +1 Query: 256 LAKVDATQEQDLAESYGVRGYPTLKFFRNGSPI-DYSGGRQADDIISWLKKKTGPPAVEV 432 L +VD T+E DL Y +RGYPTL F+NG I YSG R+ D ++ +++K+ P V+ Sbjct: 75 LVEVDCTEEGDLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQL-LPTVKP 133 Query: 433 TSAEQAKELID-ANTVIVFGFFSDQ 504 S + + ++ A+ + V FF DQ Sbjct: 134 ISKDTLENFVEKADDLAVVAFFKDQ 158 Score = 63.7 bits (148), Expect = 2e-11 Identities = 29/70 (41%), Positives = 48/70 (68%), Gaps = 1/70 (1%) Frame = +2 Query: 86 TEENVLVLSKANFETVITT-TEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIN*R 262 ++E+++VL NF+ ++ T+ +LVEFYAPWCGHCK+LAP Y K A + +++ + + Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVV-A 411 Query: 263 KLTQLKNRIS 292 K+ +N IS Sbjct: 412 KIDATENDIS 421 Score = 58.8 bits (136), Expect = 5e-10 Identities = 22/45 (48%), Positives = 33/45 (73%) Frame = +2 Query: 107 LSKANFETVITTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEE 241 ++K +IT + ++V+FYAPWCGHCK+LAPEY AA +L ++ Sbjct: 27 VNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESAADELEKD 71 Score = 36.7 bits (81), Expect = 0.002 Identities = 19/53 (35%), Positives = 34/53 (64%), Gaps = 3/53 (5%) Frame = +1 Query: 256 LAKVDATQEQDLAESYGVRGYPTLKFFRNG---SPIDYSGGRQADDIISWLKK 405 +AK+DAT E D++ S + G+PT+ FF+ +P+ Y G R +D+ +++ K Sbjct: 410 VAKIDAT-ENDISVS--ISGFPTIMFFKANDKVNPVRYEGDRTLEDLSAFIDK 459 >SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 363 Score = 50.8 bits (116), Expect = 1e-07 Identities = 24/67 (35%), Positives = 31/67 (46%) Frame = +2 Query: 32 LIFTAIALLGLALGDEVPTEENVLVLSKANFETVITTTEYILVEFYAPWCGHCKSLAPEY 211 L +L+ G N + L+ NF + LV FYAPWCG+CK L P Y Sbjct: 11 LFLACFSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGYCKKLVPTY 70 Query: 212 AKAATKL 232 K A+ L Sbjct: 71 QKLASNL 77 Score = 27.9 bits (59), Expect = 1.2 Identities = 18/65 (27%), Positives = 32/65 (49%), Gaps = 6/65 (9%) Frame = +1 Query: 268 DATQEQDLAESYGVRGYPTLKFF---RNGSPI---DYSGGRQADDIISWLKKKTGPPAVE 429 DA Q + + Y V+G+PT+K GS + DY+G R + ++ P V+ Sbjct: 89 DADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDRSYKSLQKFVSDSI-PSKVK 147 Query: 430 VTSAE 444 + ++E Sbjct: 148 ILTSE 152 >SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 359 Score = 48.8 bits (111), Expect = 6e-07 Identities = 21/40 (52%), Positives = 27/40 (67%), Gaps = 1/40 (2%) Frame = +2 Query: 95 NVLVLSKANFETVITTTEY-ILVEFYAPWCGHCKSLAPEY 211 NV+ L NF+ V+ + +LVEFYA WCG+CK LAP Y Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTY 180 Score = 47.6 bits (108), Expect = 1e-06 Identities = 24/74 (32%), Positives = 39/74 (52%), Gaps = 2/74 (2%) Frame = +1 Query: 256 LAKVDATQEQDLAESYGVRGYPTLKFF-RNGS-PIDYSGGRQADDIISWLKKKTGPPAVE 429 + K+DA D+A+ Y + G+PTL +F +GS P+ YS R D + ++ +KTG + Sbjct: 76 IGKIDADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNARDVDSLTQFVSEKTGIKKRK 135 Query: 430 VTSAEQAKELIDAN 471 + EL N Sbjct: 136 IVLPSNVVELDSLN 149 Score = 46.4 bits (105), Expect = 3e-06 Identities = 24/65 (36%), Positives = 34/65 (52%) Frame = +2 Query: 17 IEMRVLIFTAIALLGLALGDEVPTEENVLVLSKANFETVITTTEYILVEFYAPWCGHCKS 196 + + +L F AL L V +++ L T+ + + L+EFYA WCGHCKS Sbjct: 1 MRLPLLSFVIFALFALVFASGVVELQSLNELEN----TIRASKKGALIEFYATWCGHCKS 56 Query: 197 LAPEY 211 LAP Y Sbjct: 57 LAPVY 61 Score = 36.3 bits (80), Expect = 0.003 Identities = 17/57 (29%), Positives = 30/57 (52%), Gaps = 3/57 (5%) Frame = +1 Query: 253 QLAKVDATQEQDLAESYGVRGYPTLKFF---RNGSPIDYSGGRQADDIISWLKKKTG 414 ++ K++A D+ + V +PT+KFF P Y G R + +I ++ KK+G Sbjct: 194 EIVKINADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSLESLIEYINKKSG 250 >SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 103 Score = 41.1 bits (92), Expect = 1e-04 Identities = 12/36 (33%), Positives = 26/36 (72%) Frame = +2 Query: 104 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAPEY 211 V + F++++ + ++V+F+A WCG CK++AP++ Sbjct: 5 VSDSSEFKSIVCQDKLVVVDFFATWCGPCKAIAPKF 40 Score = 27.1 bits (57), Expect = 2.0 Identities = 11/32 (34%), Positives = 17/32 (53%) Frame = +1 Query: 262 KVDATQEQDLAESYGVRGYPTLKFFRNGSPID 357 KVD Q ++A GV P+ ++NG I+ Sbjct: 54 KVDVDQLSEIAAEAGVHAMPSFFLYKNGEKIE 85 >SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosaccharomyces pombe|chr 2|||Manual Length = 290 Score = 39.5 bits (88), Expect = 4e-04 Identities = 15/44 (34%), Positives = 29/44 (65%) Frame = +2 Query: 104 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAPEYAKAATKLA 235 + S ++ + I + Y+ V+ YA WCG CK+++P +++ A+K A Sbjct: 6 IRSYQHWISTIPKSGYLAVDCYADWCGPCKAISPLFSQLASKYA 49 Score = 36.3 bits (80), Expect = 0.003 Identities = 23/72 (31%), Positives = 35/72 (48%), Gaps = 4/72 (5%) Frame = +1 Query: 250 YQLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGR--QA--DDIISWLKKKTGP 417 + AKV+ +++ +A GV+ PT FF NG ID G QA + + K TG Sbjct: 53 FVFAKVNVDEQRQIASGLGVKAMPTFVFFENGKQIDMLTGANPQALKEKVALISSKATGT 112 Query: 418 PAVEVTSAEQAK 453 A+ +S+ K Sbjct: 113 GALASSSSAPVK 124 >SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccharomyces pombe|chr 2|||Manual Length = 121 Score = 36.3 bits (80), Expect = 0.003 Identities = 15/34 (44%), Positives = 21/34 (61%) Frame = +2 Query: 104 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAP 205 V S ++ T I+ + +V+FYA WCG CK L P Sbjct: 22 VESFGDYNTRISADKVTVVDFYADWCGPCKYLKP 55 Score = 31.1 bits (67), Expect = 0.12 Identities = 16/47 (34%), Positives = 23/47 (48%) Frame = +1 Query: 265 VDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKK 405 V+A + D+A+ GV PT+ FR G +D G + S L K Sbjct: 72 VNADKFSDIAQKNGVYALPTMVLFRKGQELDRIVGADVKTLSSLLAK 118 >SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces pombe|chr 1|||Manual Length = 632 Score = 33.9 bits (74), Expect = 0.018 Identities = 11/64 (17%), Positives = 35/64 (54%) Frame = +1 Query: 253 QLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKKKTGPPAVEV 432 ++A+V+ +E+++ + ++ +PT + F+ I Y+G + ++S+ + A+++ Sbjct: 231 KMAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYNGPLKYQQLLSYSNQVASYQAIKI 290 Query: 433 TSAE 444 + Sbjct: 291 EEGD 294 Score = 28.7 bits (61), Expect = 0.66 Identities = 23/73 (31%), Positives = 32/73 (43%), Gaps = 8/73 (10%) Frame = +1 Query: 241 RISYQLAKVDAT---QEQDLAESYGVRGYPTLKFFRNGSPIDYSG--GRQADD---IISW 396 R+ QLA V + LA YG + P++ RNG PI Y R+ D I W Sbjct: 324 RLKLQLAGVAPLYICNSKALANKYGAQSQPSIIAVRNGMPIVYQAITPREFRDYKRITEW 383 Query: 397 LKKKTGPPAVEVT 435 + + P E+T Sbjct: 384 INIVSSPFITELT 396 >SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces pombe|chr 2|||Manual Length = 726 Score = 29.1 bits (62), Expect = 0.50 Identities = 15/84 (17%), Positives = 38/84 (45%) Frame = +1 Query: 256 LAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKKKTGPPAVEVT 435 +A ++ + + Y ++ +PT FF+ + ++Y G D++S+ ++ EV Sbjct: 333 VAHINCAVSKRACKQYSIQYFPTFLFFKEEAFVEYVGLPNEGDLVSFAEEAANFEIREVE 392 Query: 436 SAEQAKELIDANTVIVFGFFSDQS 507 + + + ++ F+ D S Sbjct: 393 LLDTVNAEKNGDVFFLY-FYDDDS 415 Score = 28.3 bits (60), Expect = 0.87 Identities = 10/28 (35%), Positives = 15/28 (53%) Frame = +2 Query: 158 VEFYAPWCGHCKSLAPEYAKAATKLAEE 241 +++Y P CG CK L P + K E+ Sbjct: 47 IKYYLPSCGACKRLGPMWDNMVEKAKEQ 74 >SPBC4B4.11 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 113 Score = 29.1 bits (62), Expect = 0.50 Identities = 14/33 (42%), Positives = 17/33 (51%) Frame = -2 Query: 558 LGHRQLEQLKGKFWLWLALVRKETKYNNSIGID 460 +G E++ GKF W L RK TK GID Sbjct: 43 IGRDTAEKVNGKFESWDGLFRKSTKTMKKEGID 75 >SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 27.9 bits (59), Expect = 1.2 Identities = 9/18 (50%), Positives = 12/18 (66%) Frame = +2 Query: 146 EYILVEFYAPWCGHCKSL 199 + IL+ FYAPW CK + Sbjct: 21 QIILLNFYAPWAAPCKQM 38 >SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9|Schizosaccharomyces pombe|chr 1|||Manual Length = 1223 Score = 27.5 bits (58), Expect = 1.5 Identities = 14/41 (34%), Positives = 19/41 (46%) Frame = +3 Query: 93 KMCSF*VKLTLKL*LQPRSTF*LNSMLHGAATANLWHRNTP 215 K +F L LQP T N +L+ + NLW R+ P Sbjct: 620 KSANFDFSFLKSLDLQPTITLGKNDLLNAILSQNLWFRSLP 660 >SPCC24B10.15 |||PINc domain|Schizosaccharomyces pombe|chr 3|||Manual Length = 462 Score = 25.4 bits (53), Expect = 6.2 Identities = 12/38 (31%), Positives = 17/38 (44%), Gaps = 5/38 (13%) Frame = -1 Query: 265 LSLVDRRFFFSQLCCCLGVF-----RCQRFAVAAPWSI 167 L ++D F S L C + RC R V PW++ Sbjct: 68 LFVLDTNFLLSHLSLCQNLIEFLTARCPRLVVVLPWTV 105 >SPCC330.08 |alg11|gmd3|alpha-1,2-mannosyltransferase Alg11|Schizosaccharomyces pombe|chr 3|||Manual Length = 471 Score = 25.0 bits (52), Expect = 8.1 Identities = 11/33 (33%), Positives = 19/33 (57%) Frame = +1 Query: 352 IDYSGGRQADDIISWLKKKTGPPAVEVTSAEQA 450 ++ SGG + D +I W+ K TG A ++ +A Sbjct: 389 VNNSGGPKFDIVIPWIGKPTGFHASTISEYAEA 421 >SPCC306.03c |cnd2||condensin subunit Cnd2|Schizosaccharomyces pombe|chr 3|||Manual Length = 742 Score = 25.0 bits (52), Expect = 8.1 Identities = 15/36 (41%), Positives = 22/36 (61%) Frame = -2 Query: 453 FSLFSRGDLNSRGASLLLQPTDDVISLTTT*IVDRT 346 F S+G+L+ SLL +D+V +TTT +VD T Sbjct: 304 FEFDSKGNLD---VSLLKSLSDEVNMITTTSLVDNT 336 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,286,569 Number of Sequences: 5004 Number of extensions: 44016 Number of successful extensions: 141 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 122 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 140 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 252150250 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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