BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30099.Seq
(730 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 236 2e-64
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 236 2e-64
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 236 2e-64
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 227 8e-62
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 36 4e-04
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.3
DQ667186-1|ABG75738.1| 447|Apis mellifera glutamate-gated chlor... 24 1.7
DQ667185-1|ABG75737.1| 447|Apis mellifera glutamate-gated chlor... 24 1.7
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 2.9
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 3.9
AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic ac... 22 5.2
AB204559-1|BAD89804.1| 832|Apis mellifera soluble guanylyl cycl... 22 6.8
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 236 bits (577), Expect = 2e-64
Identities = 114/141 (80%), Positives = 120/141 (85%)
Frame = +2
Query: 257 KFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHAL 436
KFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHAL
Sbjct: 6 KFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHAL 65
Query: 437 LAFTLGVKQLIVGVNKMDSTEPPYVSPDLRKSRRKYPHTFKKIGYNPAAVAFVPISGWHG 616
LAFTLGVKQLIVGVNKMDSTEPPY + +++ KKIGYNPAAVAFVPISGWHG
Sbjct: 66 LAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHG 125
Query: 617 DNMLELQPKCLGSRDGQVERK 679
DNMLE+ K + VERK
Sbjct: 126 DNMLEVSSKMPWFKGWTVERK 146
Score = 30.3 bits (65), Expect = 0.019
Identities = 14/22 (63%), Positives = 18/22 (81%), Gaps = 2/22 (9%)
Frame = +3
Query: 669 WSV--R*GQADGKCLIEALDAI 728
W+V + G+ +GKCLIEALDAI
Sbjct: 141 WTVERKEGKVEGKCLIEALDAI 162
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 236 bits (577), Expect = 2e-64
Identities = 114/141 (80%), Positives = 120/141 (85%)
Frame = +2
Query: 257 KFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHAL 436
KFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHAL
Sbjct: 22 KFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHAL 81
Query: 437 LAFTLGVKQLIVGVNKMDSTEPPYVSPDLRKSRRKYPHTFKKIGYNPAAVAFVPISGWHG 616
LAFTLGVKQLIVGVNKMDSTEPPY + +++ KKIGYNPAAVAFVPISGWHG
Sbjct: 82 LAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHG 141
Query: 617 DNMLELQPKCLGSRDGQVERK 679
DNMLE+ K + VERK
Sbjct: 142 DNMLEVSSKMPWFKGWTVERK 162
Score = 30.3 bits (65), Expect = 0.019
Identities = 14/22 (63%), Positives = 18/22 (81%), Gaps = 2/22 (9%)
Frame = +3
Query: 669 WSV--R*GQADGKCLIEALDAI 728
W+V + G+ +GKCLIEALDAI
Sbjct: 157 WTVERKEGKVEGKCLIEALDAI 178
Score = 27.5 bits (58), Expect = 0.14
Identities = 11/11 (100%), Positives = 11/11 (100%)
Frame = +1
Query: 196 WVLDKLKAERE 228
WVLDKLKAERE
Sbjct: 1 WVLDKLKAERE 11
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 236 bits (577), Expect = 2e-64
Identities = 114/141 (80%), Positives = 120/141 (85%)
Frame = +2
Query: 257 KFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHAL 436
KFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHAL
Sbjct: 79 KFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHAL 138
Query: 437 LAFTLGVKQLIVGVNKMDSTEPPYVSPDLRKSRRKYPHTFKKIGYNPAAVAFVPISGWHG 616
LAFTLGVKQLIVGVNKMDSTEPPY + +++ KKIGYNPAAVAFVPISGWHG
Sbjct: 139 LAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHG 198
Query: 617 DNMLELQPKCLGSRDGQVERK 679
DNMLE+ K + VERK
Sbjct: 199 DNMLEVSSKMPWFKGWTVERK 219
Score = 145 bits (352), Expect = 3e-37
Identities = 67/68 (98%), Positives = 67/68 (98%)
Frame = +1
Query: 25 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 204
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 205 DKLKAERE 228
DKLKAERE
Sbjct: 61 DKLKAERE 68
Score = 30.3 bits (65), Expect = 0.019
Identities = 14/22 (63%), Positives = 18/22 (81%), Gaps = 2/22 (9%)
Frame = +3
Query: 669 WSV--R*GQADGKCLIEALDAI 728
W+V + G+ +GKCLIEALDAI
Sbjct: 214 WTVERKEGKVEGKCLIEALDAI 235
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 227 bits (555), Expect = 8e-62
Identities = 109/141 (77%), Positives = 118/141 (83%)
Frame = +2
Query: 257 KFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHAL 436
KFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG GEFEAGISKNGQTREHAL
Sbjct: 79 KFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGEFEAGISKNGQTREHAL 138
Query: 437 LAFTLGVKQLIVGVNKMDSTEPPYVSPDLRKSRRKYPHTFKKIGYNPAAVAFVPISGWHG 616
LAFTLGVKQLIVGVNKMD T+PPY + +++ KKIGYN A+VAFVPISGWHG
Sbjct: 139 LAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFVPISGWHG 198
Query: 617 DNMLELQPKCLGSRDGQVERK 679
DNMLE PK + +VERK
Sbjct: 199 DNMLEPSPKTPWYKGWKVERK 219
Score = 145 bits (352), Expect = 3e-37
Identities = 67/68 (98%), Positives = 67/68 (98%)
Frame = +1
Query: 25 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 204
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 205 DKLKAERE 228
DKLKAERE
Sbjct: 61 DKLKAERE 68
Score = 28.7 bits (61), Expect = 0.059
Identities = 13/15 (86%), Positives = 13/15 (86%)
Frame = +3
Query: 684 GQADGKCLIEALDAI 728
G ADGK LIEALDAI
Sbjct: 221 GNADGKTLIEALDAI 235
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 35.9 bits (79), Expect = 4e-04
Identities = 35/118 (29%), Positives = 51/118 (43%)
Frame = +2
Query: 281 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 460
VT +D PGH FI G D VL+VAA G E QT + +A V
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV- 246
Query: 461 QLIVGVNKMDSTEPPYVSPDLRKSRRKYPHTFKKIGYNPAAVAFVPISGWHGDNMLEL 634
+IV +NK+D + ++ K+ +++G + V IS G N+ EL
Sbjct: 247 PIIVAINKIDKPNIDIIK--VQYELAKHGIVIEELG---GEIQCVKISALKGINLREL 299
Score = 25.4 bits (53), Expect = 0.55
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 37 KTHINIVVIGHVDSGKST 90
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.3
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +1
Query: 46 INIVVIGHVDSGKST 90
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>DQ667186-1|ABG75738.1| 447|Apis mellifera glutamate-gated chloride
channel protein.
Length = 447
Score = 23.8 bits (49), Expect = 1.7
Identities = 12/54 (22%), Positives = 25/54 (46%)
Frame = +2
Query: 215 RLSVXRYHNRYCSRKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAA 376
R ++ ++ YC+ K T +Y +D R+F +I C +L++ +
Sbjct: 208 RFTLEKFFTDYCNSKTNTGEYSCLKVDLLFKREFSYYLI--QIYIPCCMLVIVS 259
>DQ667185-1|ABG75737.1| 447|Apis mellifera glutamate-gated chloride
channel protein.
Length = 447
Score = 23.8 bits (49), Expect = 1.7
Identities = 12/54 (22%), Positives = 25/54 (46%)
Frame = +2
Query: 215 RLSVXRYHNRYCSRKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAA 376
R ++ ++ YC+ K T +Y +D R+F +I C +L++ +
Sbjct: 208 RFTLEKFFTDYCNSKTNTGEYSCLKVDLLFKREFSYYLI--QIYIPCCMLVIVS 259
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 2.9
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +2
Query: 266 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 367
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 3.9
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +2
Query: 266 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 367
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
>AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic
acetylcholine Apisa7-2 subunit protein.
Length = 461
Score = 22.2 bits (45), Expect = 5.2
Identities = 9/29 (31%), Positives = 14/29 (48%)
Frame = -1
Query: 658 P*TKAFWLKLQHVVSVPSRNGHESDSSWV 572
P F L L H++ V +N + + WV
Sbjct: 30 PLAVVFGLSLHHIIDVDEKNQILTTNCWV 58
>AB204559-1|BAD89804.1| 832|Apis mellifera soluble guanylyl cyclase
beta-3 protein.
Length = 832
Score = 21.8 bits (44), Expect = 6.8
Identities = 6/14 (42%), Positives = 11/14 (78%)
Frame = +1
Query: 601 FWMARRQHVGASTK 642
+W+ +R+H +STK
Sbjct: 593 YWLEKREHRSSSTK 606
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 203,507
Number of Sequences: 438
Number of extensions: 4178
Number of successful extensions: 30
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 18
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 26
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 22657590
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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