BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ps4M0746.Seq (875 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr 1|... 58 2e-09 SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr 3|||Ma... 32 0.093 SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun... 27 3.5 SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 27 3.5 >SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr 1|||Manual Length = 137 Score = 58.0 bits (134), Expect = 2e-09 Identities = 26/57 (45%), Positives = 37/57 (64%) Frame = +3 Query: 312 KQKLFLMSWCPVTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEASQEAVEEKL 482 + K+ +SW P A +K KM+YSSS D L+++ G+ IQATD SE + E V EK+ Sbjct: 78 RNKIIFISWSPDVAPIKSKMVYSSSKDTLRRAFTGIGTDIQATDFSEVAYETVLEKV 134 Score = 39.9 bits (89), Expect = 5e-04 Identities = 21/54 (38%), Positives = 29/54 (53%) Frame = +1 Query: 64 SGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDL 225 SGV VS C ++E+K K RYVVF + D K V + +++ FL DL Sbjct: 4 SGVKVSPECLEAFQELKLGKSLRYVVFKMNDTKTEIVVEKKSTDKDFDTFLGDL 57 >SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr 3|||Manual Length = 328 Score = 32.3 bits (70), Expect = 0.093 Identities = 15/27 (55%), Positives = 20/27 (74%) Frame = +3 Query: 306 SKKQKLFLMSWCPVTAKVKKKMLYSSS 386 SKK L L+S+ P A V++KMLY+SS Sbjct: 76 SKKNLLQLISYVPENANVRRKMLYASS 102 Score = 30.3 bits (65), Expect = 0.38 Identities = 16/56 (28%), Positives = 33/56 (58%), Gaps = 5/56 (8%) Frame = +3 Query: 318 KLFLMSWCPVTAKVKKKMLYSSS----FDALKKSL-VGVQKYIQATDLSEASQEAV 470 K+ + CP+ A VK +M+YSSS D++K L + + I++ D ++ +++ + Sbjct: 244 KILFIYICPMQATVKHRMVYSSSKLGLLDSIKAELGIVIDGKIESNDAADITEKEI 299 >SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 214 Score = 27.1 bits (57), Expect = 3.5 Identities = 10/26 (38%), Positives = 19/26 (73%) Frame = +3 Query: 384 SFDALKKSLVGVQKYIQATDLSEASQ 461 S +AL++ L G + Y+Q+TD++ S+ Sbjct: 188 SLEALQEELEGFEDYVQSTDIAAMSK 213 >SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosaccharomyces pombe|chr 1|||Manual Length = 1002 Score = 27.1 bits (57), Expect = 3.5 Identities = 17/63 (26%), Positives = 25/63 (39%) Frame = +2 Query: 398 EKVPCRSSEVHPSDRPLGSVSGGRRREAPRHRSPINSFTHELATKPNPLSDTPALNDTRP 577 EK P S++ R G G + +H +H P P D+ +D RP Sbjct: 131 EKRPHDSADEDAPRRKHGKCKGKGKHHKGKHAKGKGKKSH-----PKPEDDSVFFDDERP 185 Query: 578 RHH 586 +HH Sbjct: 186 KHH 188 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,953,689 Number of Sequences: 5004 Number of extensions: 52961 Number of successful extensions: 143 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 137 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 143 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 438479610 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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