Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ps4M0737.Seq
         (829 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBP8B7.27 |mug30||ubiquitin-protein ligase E3|Schizosaccharomyc...    76   7e-15
SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomy...    56   6e-09
SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyc...    54   3e-08
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo...    48   2e-06
SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa...    42   1e-04
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||...    40   4e-04
SPCC126.08c |||lectin |Schizosaccharomyces pombe|chr 3|||Manual        29   1.1  
SPBC83.06c |||mitochondrial ribosomal protein subunit L36|Schizo...    27   2.5  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    27   2.5  
SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ...    27   4.3  
SPAP27G11.04c |||tRNA specific adenosine deaminase subunit Tad3 ...    26   7.5  
SPBC1709.01 |chs2|SPBC1734.17|chitin synthase homolog Chs2|Schiz...    26   7.5  
SPAC57A7.11 |mip1||WD repeat protein Mip1|Schizosaccharomyces po...    25   9.9  
SPAC343.04c |gnr1||heterotrimeric G protein beta subunit Gnr1|Sc...    25   9.9  

>SPBP8B7.27 |mug30||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 807

 Score = 75.8 bits (178), Expect = 7e-15
 Identities = 41/122 (33%), Positives = 71/122 (58%), Gaps = 6/122 (4%)
 Frame = -2

Query: 348 RRCKTTASEL*YDSL*GELGTGPPLEEPYRQDTMLQIS----NHDTSQLKKPLRVEFIGE 181
           R+ +  A E  + S+  ++   P L    R+D +L+ S    N      +K L+VEF+GE
Sbjct: 409 RKMEIKAREAFFSSILSKMNVEPYLMIRVRRDRLLEDSLRQINDRNKDFRKALKVEFLGE 468

Query: 180 EAEDAGGVKKEFFMLLLKEIFDPVYGMFKQSEE-TNMIWFSNNPFEDDVMYY-VIGAIYG 7
           E  DAGG+K+E+ +LL +++F P +G+F   EE +N +WF+ +    ++ YY + G + G
Sbjct: 469 EGIDAGGLKREWLLLLTRKVFSPEFGLFVNCEESSNYLWFNYSHRSKEIDYYHMSGILMG 528

Query: 6   LA 1
           +A
Sbjct: 529 IA 530


>SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 786

 Score = 56.0 bits (129), Expect = 6e-09
 Identities = 32/91 (35%), Positives = 49/91 (53%), Gaps = 5/91 (5%)
 Frame = -2

Query: 258 QDTMLQISNHDTSQLKKPLRVEFIGEEAEDAGGVKKEFFMLLLKEIFDPVYGMFKQSEET 79
           +D+  +I  +    LKK L + F GE+  D GG+ +EFF LL  ++FDP+Y +F+ S   
Sbjct: 443 EDSYAEIMRYSAHDLKKRLMIRFDGEDGLDYGGLSREFFFLLSHKMFDPIYCLFEYSAVD 502

Query: 78  NMIWFSNNPFE----DDVMYY-VIGAIYGLA 1
           N      NP      + + Y+  IG + GLA
Sbjct: 503 NYT-LQINPHSSINPEHLNYFRFIGRVIGLA 532


>SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 767

 Score = 53.6 bits (123), Expect = 3e-08
 Identities = 31/91 (34%), Positives = 49/91 (53%), Gaps = 5/91 (5%)
 Frame = -2

Query: 258 QDTMLQISNHDTSQLKKPLRVEFIGEEAEDAGGVKKEFFMLLLKEIFDPVYGMFKQSEET 79
           +D+  +I     + LKK L ++F GE+  D GG+ +E+F LL  E+F+P Y +F+ S   
Sbjct: 424 EDSYAEIMRQSATDLKKRLMIKFDGEDGLDYGGLSREYFFLLSHEMFNPFYCLFEYSSVD 483

Query: 78  NMIWFSNNPFE----DDVMYY-VIGAIYGLA 1
           N      NP      + + Y+  IG + GLA
Sbjct: 484 NYT-LQINPHSGINPEHLNYFKFIGRVIGLA 513


>SPAC1805.15c |pub2||ubiquitin-protein ligase
           Pub2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 671

 Score = 48.0 bits (109), Expect = 2e-06
 Identities = 22/55 (40%), Positives = 32/55 (58%)
 Frame = -2

Query: 240 ISNHDTSQLKKPLRVEFIGEEAEDAGGVKKEFFMLLLKEIFDPVYGMFKQSEETN 76
           IS    S +KK L + F  E+  D GGV +EFF +L   IF+P Y +F+ + + N
Sbjct: 334 ISKLSVSDMKKKLLIRFRNEDGLDYGGVSREFFYILSHAIFNPGYSLFEYATDDN 388


>SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1029

 Score = 41.9 bits (94), Expect = 1e-04
 Identities = 21/65 (32%), Positives = 38/65 (58%), Gaps = 5/65 (7%)
 Frame = -2

Query: 216 LKKPLRVEFIGE-----EAEDAGGVKKEFFMLLLKEIFDPVYGMFKQSEETNMIWFSNNP 52
           LK P+R+ F+ E     E  D GG+ KEF   + K +FD  YG+F ++ + ++++ + + 
Sbjct: 696 LKGPIRITFVDEHGVVEEGIDGGGLTKEFLTSICKTVFDINYGLFSET-KAHLLYPNTHA 754

Query: 51  FEDDV 37
           +  DV
Sbjct: 755 YAQDV 759


>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 3227

 Score = 39.9 bits (89), Expect = 4e-04
 Identities = 23/68 (33%), Positives = 36/68 (52%), Gaps = 6/68 (8%)
 Frame = -2

Query: 282  PPLEEPYRQDTMLQISNH-----DTSQLK-KPLRVEFIGEEAEDAGGVKKEFFMLLLKEI 121
            PPL    R+D +   S       D  ++K   L + F  EE  DAGGV +E+  +L +++
Sbjct: 2868 PPLNITVRRDHVFLDSYRALHFKDADEVKFSKLNIHFRDEEGVDAGGVTREWLQVLARQM 2927

Query: 120  FDPVYGMF 97
            F+P Y +F
Sbjct: 2928 FNPDYALF 2935


>SPCC126.08c |||lectin |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 312

 Score = 28.7 bits (61), Expect = 1.1
 Identities = 13/39 (33%), Positives = 19/39 (48%)
 Frame = +1

Query: 166 GIFRFFANKLDSERFLQL*SVMVAYLQHGVLTIWFLEGG 282
           GIF F    L     L+  S+   Y+ HG+  +W+  GG
Sbjct: 12  GIFGFVLGALAETSHLERLSLEAPYINHGMRNLWWEYGG 50


>SPBC83.06c |||mitochondrial ribosomal protein subunit
           L36|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 59

 Score = 27.5 bits (58), Expect = 2.5
 Identities = 13/34 (38%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
 Frame = -3

Query: 173 KMPVALKKN-SSCYCSRKYLIPYTVCSSNPRRRT 75
           K+  ++KK  SSCY  R+    Y +C  +PR +T
Sbjct: 23  KVKASVKKRCSSCYFVRRKGRLYVLCKKHPRHKT 56


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 27.5 bits (58), Expect = 2.5
 Identities = 23/78 (29%), Positives = 37/78 (47%), Gaps = 8/78 (10%)
 Frame = +3

Query: 255 PDDMVPRGGARY---PIRPIVSRITIHWPSFYNVVTGKTLALPNLIALQ-----HIPLSP 410
           P+  V  GG+     P  P+ S ++ H   +    +    +L N I+L      ++PLSP
Sbjct: 157 PETPVSPGGSLVHPLPRPPLPSSVSSHSSPYSTTSSTSLYSLYNDISLSCSPEPYLPLSP 216

Query: 411 AGVIAKRPAPIALPNSCA 464
               A+ P+PI L +S A
Sbjct: 217 TRSPARTPSPIRLYSSDA 234


>SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 962

 Score = 26.6 bits (56), Expect = 4.3
 Identities = 9/27 (33%), Positives = 17/27 (62%)
 Frame = -2

Query: 135 LLKEIFDPVYGMFKQSEETNMIWFSNN 55
           L+K   + ++G  K +E+T +IW + N
Sbjct: 562 LVKHFMNSLFGRVKLNEQTEIIWHTRN 588


>SPAP27G11.04c |||tRNA specific adenosine deaminase subunit Tad3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 315

 Score = 25.8 bits (54), Expect = 7.5
 Identities = 12/33 (36%), Positives = 20/33 (60%)
 Frame = -2

Query: 300 GELGTGPPLEEPYRQDTMLQISNHDTSQLKKPL 202
           GE+G    + +P   DT+L +S  + S+LK P+
Sbjct: 178 GEIGCAAAIYDP-TTDTVLAVSVDERSKLKNPI 209


>SPBC1709.01 |chs2|SPBC1734.17|chitin synthase homolog
           Chs2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 926

 Score = 25.8 bits (54), Expect = 7.5
 Identities = 11/27 (40%), Positives = 13/27 (48%)
 Frame = -3

Query: 473 PFXCATVGKGDRCGPLRYYASWRKGDV 393
           P  C  V  G R GP   Y +W+  DV
Sbjct: 392 PKVCLFVRNGARLGPTSIYHAWKAFDV 418


>SPAC57A7.11 |mip1||WD repeat protein Mip1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1313

 Score = 25.4 bits (53), Expect = 9.9
 Identities = 9/26 (34%), Positives = 14/26 (53%)
 Frame = -3

Query: 338 KRRPVNCNTTHYRANWVPGPPSRNHI 261
           + +P   N  H  +  VPGPP+ N +
Sbjct: 886 ENKPAESNLNHLTSAKVPGPPAFNEL 911


>SPAC343.04c |gnr1||heterotrimeric G protein beta subunit
           Gnr1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 507

 Score = 25.4 bits (53), Expect = 9.9
 Identities = 12/34 (35%), Positives = 20/34 (58%)
 Frame = +2

Query: 608 ECCSSLEQESTIKETWTSNVKRAKNRLIRGGWXT 709
           E   +LE+E+ +    T +VKR K  +++G W T
Sbjct: 26  ESLKALEKETGLVSE-TEDVKRLKQAVLQGDWIT 58


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,466,539
Number of Sequences: 5004
Number of extensions: 71713
Number of successful extensions: 165
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 165
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 406444570
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)