Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ps4M0737.Seq
(829 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearl... 42 5e-04
At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearl... 42 5e-04
At3g17205.1 68416.m02196 HECT-domain-containing protein / ubiqui... 37 0.014
At3g53090.1 68416.m05851 HECT-domain-containing protein / ubiqui... 33 0.18
At4g12570.1 68417.m01983 ubiquitin-protein ligase, putative simi... 32 0.40
At5g17390.1 68418.m02040 universal stress protein (USP) family p... 30 2.2
At5g08750.1 68418.m01039 zinc finger (C3HC4-type RING finger) fa... 30 2.2
At4g28420.1 68417.m04068 aminotransferase, putative tsimilar to ... 28 6.6
At5g44630.1 68418.m05468 terpene synthase/cyclase family protein 28 8.7
At2g18760.1 68415.m02184 SNF2 domain-containing protein / helica... 28 8.7
>At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearly
identical to ubiquitin-protein ligase 2 [Arabidopsis
thaliana] GI:7108523; E3, HECT-domain protein family;
similar to ubiquitin-protein ligase 2 GI:7108523 from
[Arabidopsis thaliana]
Length = 3658
Score = 41.9 bits (94), Expect = 5e-04
Identities = 20/48 (41%), Positives = 29/48 (60%)
Frame = -2
Query: 258 QDTMLQISNHDTSQLKKPLRVEFIGEEAEDAGGVKKEFFMLLLKEIFD 115
+D+ Q+ LK L V+F GEE DAGG+ +E++ LL + IFD
Sbjct: 3307 EDSYNQLRMRSPQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFD 3354
>At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearly
identical to ubiquitin-protein ligase 1 [Arabidopsis
thaliana] GI:7108521; E3, HECT-domain protein family;
similar to GI:7108521, GB:AAF36454 from [Arabidopsis
thaliana]
Length = 3891
Score = 41.9 bits (94), Expect = 5e-04
Identities = 20/48 (41%), Positives = 29/48 (60%)
Frame = -2
Query: 258 QDTMLQISNHDTSQLKKPLRVEFIGEEAEDAGGVKKEFFMLLLKEIFD 115
+D+ Q+ LK L V+F GEE DAGG+ +E++ LL + IFD
Sbjct: 3540 EDSYNQLRMRSPQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFD 3587
>At3g17205.1 68416.m02196 HECT-domain-containing protein /
ubiquitin-transferase family protein weak similarity to
ubiquitin-protein ligase 2 [Arabidopsis thaliana]
GI:7108523; contains Pfam profile PF00632: HECT-domain
(ubiquitin-transferase)
Length = 873
Score = 37.1 bits (82), Expect = 0.014
Identities = 21/64 (32%), Positives = 34/64 (53%), Gaps = 5/64 (7%)
Frame = -2
Query: 258 QDTMLQISNHDTSQLKKPLRVEFIGE----EAE-DAGGVKKEFFMLLLKEIFDPVYGMFK 94
+D Q+S L+ +RV F+ E EA D GG+ K+F + + FD YG+FK
Sbjct: 522 EDAYNQMSALSEDDLRSSIRVTFVNELGVEEAGIDGGGIFKDFMEKITRAAFDVQYGLFK 581
Query: 93 QSEE 82
++ +
Sbjct: 582 ETAD 585
>At3g53090.1 68416.m05851 HECT-domain-containing protein /
ubiquitin-transferase family protein / IQ
calmodulin-binding motif-containing protein contains
Pfam profiles PF00632: HECT-domain
(ubiquitin-transferase), PF00612: IQ calmodulin-binding
motif
Length = 1142
Score = 33.5 bits (73), Expect = 0.18
Identities = 20/56 (35%), Positives = 30/56 (53%), Gaps = 5/56 (8%)
Frame = -2
Query: 222 SQLKKPLRVEFIGE----EAE-DAGGVKKEFFMLLLKEIFDPVYGMFKQSEETNMI 70
S+LK + V F+ E EA D GG+ KEF + K F YG+F Q+ ++ +
Sbjct: 796 SRLKSSIHVSFVNESGLPEAGLDYGGLSKEFLTDITKAAFATEYGLFSQTPTSDRL 851
>At4g12570.1 68417.m01983 ubiquitin-protein ligase, putative similar
to SP|P39940 Ubiquitin--protein ligase RSP5 (EC 6.3.2.-)
{Saccharomyces cerevisiae}; contains Pfam profiles
PF00240: Ubiquitin family, PF00632: HECT-domain
(ubiquitin-transferase)
Length = 873
Score = 32.3 bits (70), Expect = 0.40
Identities = 21/51 (41%), Positives = 31/51 (60%)
Frame = -2
Query: 204 LRVEFIGEEAEDAGGVKKEFFMLLLKEIFDPVYGMFKQSEETNMIWFSNNP 52
L +EF EEA G V +E+F L+ +EIF+P +F +S + + FS NP
Sbjct: 540 LFMEFKNEEATGPG-VLREWFYLVCQEIFNPKNTLFLRSAD-DFRRFSPNP 588
>At5g17390.1 68418.m02040 universal stress protein (USP) family
protein contains Pfam profile: PF00582 universal stress
protein family
Length = 285
Score = 29.9 bits (64), Expect = 2.2
Identities = 10/27 (37%), Positives = 20/27 (74%)
Frame = -2
Query: 228 DTSQLKKPLRVEFIGEEAEDAGGVKKE 148
D+S +++ R+EF+GE +DAG +++
Sbjct: 28 DSSSIERDQRIEFLGENGDDAGSGEED 54
>At5g08750.1 68418.m01039 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 363
Score = 29.9 bits (64), Expect = 2.2
Identities = 18/64 (28%), Positives = 29/64 (45%)
Frame = -2
Query: 363 GFSQSRRCKTTASEL*YDSL*GELGTGPPLEEPYRQDTMLQISNHDTSQLKKPLRVEFIG 184
G R C+ S+ D+ G LG PP+ EP R + + + +S +K +E I
Sbjct: 39 GLPTCRVCQCAESDRRGDAALGFLGITPPVSEPRRSNAGEETVDQRSSVVKSSGFIELIS 98
Query: 183 EEAE 172
+ E
Sbjct: 99 PDGE 102
>At4g28420.1 68417.m04068 aminotransferase, putative tsimilar to
nicotianamine aminotransferase from Hordeum vulgare
[GI:6498122, GI:6469087]; contains Pfam profile PF00155
aminotransferase, classes I and II
Length = 389
Score = 28.3 bits (60), Expect = 6.6
Identities = 16/44 (36%), Positives = 20/44 (45%)
Frame = -3
Query: 206 RSESSLLAKKRKMPVALKKNSSCYCSRKYLIPYTVCSSNPRRRT 75
++ L AKK M LK+N C R IP VC+ P T
Sbjct: 324 KANKELFAKKNSM---LKQNVELVCDRLKEIPCLVCNKKPESCT 364
>At5g44630.1 68418.m05468 terpene synthase/cyclase family protein
Length = 557
Score = 27.9 bits (59), Expect = 8.7
Identities = 17/56 (30%), Positives = 25/56 (44%)
Frame = +1
Query: 7 SVNGPNHIIHDVIFEWII*KPNHVRLLGLLEHTVYGIKYFLEQ*HEEFFFNATGIF 174
S+ G I FEW+I +P VR+LG + I F E + + NA +
Sbjct: 420 SIMGLGEIGKKEAFEWLISRPKLVRILGAKTRLMDDIADFEEDMEKGYTANALNYY 475
>At2g18760.1 68415.m02184 SNF2 domain-containing protein / helicase
domain-containing protein similar to SP|Q03468 Excision
repair protein ERCC-6 (Cockayne syndrome protein CSB)
{Homo sapiens}; contains PFam profiles PF00271: Helicase
conserved C-terminal domain, PF00176: SNF2 family
N-terminal domain
Length = 1187
Score = 27.9 bits (59), Expect = 8.7
Identities = 16/55 (29%), Positives = 24/55 (43%)
Frame = +3
Query: 300 PIVSRITIHWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPNSCA 464
PI +++T W F V GK LP A +P++ G P ++ CA
Sbjct: 576 PIQNKLTELWSLFDFVFPGKLGVLPVFEAEFSVPITVGGYANASPLQVSTAYRCA 630
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,465,714
Number of Sequences: 28952
Number of extensions: 391760
Number of successful extensions: 953
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 922
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 953
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1902108000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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