Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ps4M0687.Seq
(911 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 163 2e-41
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 163 2e-41
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 163 2e-41
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 49 8e-07
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 48 1e-06
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 40 6e-04
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 30 0.40
SPBC11B10.05c |rsp1||random septum position protein Rsp1|Schizos... 30 0.40
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 29 1.2
SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr 1|||Ma... 28 1.6
SPAC23D3.08 |usp108||U1 snRNP-associated protein Usp108|Schizosa... 27 3.7
SPAC14C4.12c |||SWIRM domain protein|Schizosaccharomyces pombe|c... 27 3.7
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 4.9
SPBC18H10.20c |||conserved fungal protein|Schizosaccharomyces po... 27 4.9
SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 26 6.5
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 163 bits (397), Expect = 2e-41
Identities = 75/111 (67%), Positives = 87/111 (78%)
Frame = -1
Query: 587 QEIKKEVSSYIKKNWLQPSCCRFRAHSGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKC 408
+EI KE S++IKK P F SG+ GDNM+EP+T MPW++GWQ E K G GK
Sbjct: 166 EEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKT 225
Query: 407 LIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIV 255
L+EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV
Sbjct: 226 LLEAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIV 276
Score = 149 bits (361), Expect = 5e-37
Identities = 66/84 (78%), Positives = 74/84 (88%)
Frame = -3
Query: 252 FAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAAD 73
FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A
Sbjct: 278 FAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCAS 337
Query: 72 FTAQVIVLNHPGQISNGYTPVLDC 1
FTAQVI+LNHPGQIS GY+PVLDC
Sbjct: 338 FTAQVIILNHPGQISAGYSPVLDC 361
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 163 bits (397), Expect = 2e-41
Identities = 75/111 (67%), Positives = 87/111 (78%)
Frame = -1
Query: 587 QEIKKEVSSYIKKNWLQPSCCRFRAHSGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKC 408
+EI KE S++IKK P F SG+ GDNM+EP+T MPW++GWQ E K G GK
Sbjct: 166 EEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKT 225
Query: 407 LIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIV 255
L+EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV
Sbjct: 226 LLEAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIV 276
Score = 149 bits (361), Expect = 5e-37
Identities = 66/84 (78%), Positives = 74/84 (88%)
Frame = -3
Query: 252 FAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAAD 73
FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A
Sbjct: 278 FAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCAS 337
Query: 72 FTAQVIVLNHPGQISNGYTPVLDC 1
FTAQVI+LNHPGQIS GY+PVLDC
Sbjct: 338 FTAQVIILNHPGQISAGYSPVLDC 361
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 163 bits (397), Expect = 2e-41
Identities = 75/111 (67%), Positives = 87/111 (78%)
Frame = -1
Query: 587 QEIKKEVSSYIKKNWLQPSCCRFRAHSGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKC 408
+EI KE S++IKK P F SG+ GDNM+EP+T MPW++GWQ E K G GK
Sbjct: 166 EEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKT 225
Query: 407 LIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIV 255
L+EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV
Sbjct: 226 LLEAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIV 276
Score = 149 bits (361), Expect = 5e-37
Identities = 66/84 (78%), Positives = 74/84 (88%)
Frame = -3
Query: 252 FAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAAD 73
FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A
Sbjct: 278 FAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCAS 337
Query: 72 FTAQVIVLNHPGQISNGYTPVLDC 1
FTAQVI+LNHPGQIS GY+PVLDC
Sbjct: 338 FTAQVIILNHPGQISAGYSPVLDC 361
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 49.2 bits (112), Expect = 8e-07
Identities = 24/49 (48%), Positives = 32/49 (65%), Gaps = 1/49 (2%)
Frame = -1
Query: 407 LIEALDAILP-PARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPG 264
L+EA+D+ + P R TD P + ++DV+ I G GTV GRVE G LK G
Sbjct: 234 LMEAVDSYITLPERKTDVPFLMAIEDVFSISGRGTVVTGRVERGTLKKG 282
Score = 35.1 bits (77), Expect = 0.014
Identities = 14/43 (32%), Positives = 27/43 (62%)
Frame = -3
Query: 243 ANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYV 115
+++ T V +EM + L AV GDN G ++++ ++L+RG +
Sbjct: 292 SHLKTTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMI 334
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 48.4 bits (110), Expect = 1e-06
Identities = 41/111 (36%), Positives = 54/111 (48%), Gaps = 3/111 (2%)
Frame = -1
Query: 602 ED*FSQEIKKEVSSY-IKKNWLQPSCCRFRAHSGWHGDNMLEPSTK--MPWFKGWQVERK 432
ED F QEIK VS + IK + S F S G N+++ + W+KG
Sbjct: 332 EDRF-QEIKNIVSDFLIKMVGFKTSNVHFVPISAISGTNLIQKDSSDLYKWYKG------ 384
Query: 431 EGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETG 279
L+ ALD ++PP +P KPLRL + DVY+ TV GRVE G
Sbjct: 385 ------PTLLSALDQLVPPEKPYRKPLRLSIDDVYRSPRSVTV-TGRVEAG 428
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 39.5 bits (88), Expect = 6e-04
Identities = 24/81 (29%), Positives = 41/81 (50%), Gaps = 1/81 (1%)
Frame = -3
Query: 246 PANITTEVKSV-EMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADF 70
P N T EV ++ + E + ++ GD V V+ +++ GYV +KN P F
Sbjct: 503 PINQTLEVTAIYDEADEEISSSICGDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRF 560
Query: 69 TAQVIVLNHPGQISNGYTPVL 7
AQ+ +L P ++ GY+ V+
Sbjct: 561 IAQIAILELPSILTTGYSCVM 581
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 30.3 bits (65), Expect = 0.40
Identities = 21/68 (30%), Positives = 30/68 (44%)
Frame = -1
Query: 467 MPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRV 288
+P K V K GK + L E + I PP + PLR L D + G + + R+
Sbjct: 206 IPMSKPILVSSKTGKNVEQILPEIIQKIPPPKGSENAPLRCLLIDSWYNSYQGVIGLVRI 265
Query: 287 ETGVLKPG 264
G +K G
Sbjct: 266 MEGFIKKG 273
>SPBC11B10.05c |rsp1||random septum position protein
Rsp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 494
Score = 30.3 bits (65), Expect = 0.40
Identities = 16/45 (35%), Positives = 24/45 (53%), Gaps = 3/45 (6%)
Frame = -1
Query: 575 KEVS---SYIKKNWLQPSCCRFRAHSGWHGDNMLEPSTKMPWFKG 450
KEVS SYI +L P +G+ +N+++P + P FKG
Sbjct: 159 KEVSTSKSYISSGYLHPKTSPIFKKNGYATENVVDPISSSPRFKG 203
>SPBC1539.08 |||ADP-ribosylation factor, Arf
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 28.7 bits (61), Expect = 1.2
Identities = 17/47 (36%), Positives = 25/47 (53%)
Frame = -2
Query: 538 NPAAVAFVPILDGTETTCWSLQPKCLGSRDGRWSVRKAKLTENASLK 398
+PA + V LD + W++QP C + DG A L++NA LK
Sbjct: 139 SPAQITDVLQLDKLKDRLWNVQPTCALTGDGLLE-GLAWLSQNAKLK 184
>SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 835
Score = 28.3 bits (60), Expect = 1.6
Identities = 15/46 (32%), Positives = 19/46 (41%)
Frame = +1
Query: 367 GRAGGRMASRASMRHFPSALPSLRSTCHPLNQGILVEGSNMLSPCH 504
G GG S+ P+ LPS + P L E +ML CH
Sbjct: 545 GAGGGHAPDIISLVQNPNILPSSTNPTRPFTTNTLDEELDMLMVCH 590
>SPAC23D3.08 |usp108||U1 snRNP-associated protein
Usp108|Schizosaccharomyces pombe|chr 1|||Manual
Length = 382
Score = 27.1 bits (57), Expect = 3.7
Identities = 23/76 (30%), Positives = 32/76 (42%), Gaps = 3/76 (3%)
Frame = +1
Query: 127 TQFLDGHVLYVET---YIVSRYSFLESFVVHLHRLDFSSDVGGGKETMVPGFNTPVSTLP 297
T +D V+ +ET YI+SR SF+ V + D + + G T + P
Sbjct: 162 TNEIDASVV-IETLLKYILSRISFIPRVSVVPYIPDAAYGIYGSPSTFTQFNHMSSMVTP 220
Query: 298 TGTVPIPPILYTSCRG 345
IPPI TS G
Sbjct: 221 ENPYGIPPIALTSAGG 236
>SPAC14C4.12c |||SWIRM domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 297
Score = 27.1 bits (57), Expect = 3.7
Identities = 19/57 (33%), Positives = 27/57 (47%)
Frame = -3
Query: 615 GPPPRRLVLSGNQEGSILIHQEELATTQLLSLSCPFWMARRQHVGAFNQNALVQGMA 445
GPP L LS N E L+H EL L L C ++ ++ + A N QG++
Sbjct: 213 GPP---LDLS-NDENRHLLHPAELQLAATLRLPCLVYLDNKRRIFAEWHNRRTQGLS 265
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 4.9
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +1
Query: 130 QFLDGHVLYVETYIVSRYSFLESFV 204
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPBC18H10.20c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 361
Score = 26.6 bits (56), Expect = 4.9
Identities = 13/37 (35%), Positives = 17/37 (45%)
Frame = +2
Query: 167 TLSPGTASWRASWCISTDLTSVVMLAGAKKQWYLVST 277
T PGT W SW L + V K ++YL +T
Sbjct: 101 TYRPGTQHWPFSWLFPGSLPASVSNRYIKLEYYLEAT 137
>SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 26.2 bits (55), Expect = 6.5
Identities = 22/72 (30%), Positives = 25/72 (34%), Gaps = 5/72 (6%)
Frame = +1
Query: 265 PGFNTPVSTLPTGTVPIP-PILYTSCR--GRRRGLSVGRAGGRMASRASMRH--FPSALP 429
P +N P G+VP+P P S R R S GR S H PS LP
Sbjct: 314 PNYNAPFQPAGVGSVPLPAPTSSQSLRLGSLHRSRSPSPRSGRPRRSPSPSHLSIPSTLP 373
Query: 430 SLRSTCHPLNQG 465
P G
Sbjct: 374 PADGVPKPTPDG 385
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,744,004
Number of Sequences: 5004
Number of extensions: 79009
Number of successful extensions: 206
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 190
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 203
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 462505890
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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