Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ps4M0641.Seq
         (578 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g22740.1 68416.m02868 homocysteine S-methyltransferase 3 (HMT...    49   3e-06
At3g63250.2 68416.m07106 homocysteine S-methyltransferase 2 (HMT...    43   1e-04
At3g63250.1 68416.m07107 homocysteine S-methyltransferase 2 (HMT...    43   1e-04
At3g25900.1 68416.m03228 homocysteine S-methyltransferase 1 (HMT...    41   7e-04
At3g53720.1 68416.m05934 cation/hydrogen exchanger, putative (CH...    28   5.2  
At3g52330.1 68416.m05751 F-box family protein-related contains w...    27   6.8  
At1g30825.1 68414.m03769 actin-related protein 2/3 complex 34kDa...    27   9.0  

>At3g22740.1 68416.m02868 homocysteine S-methyltransferase 3 (HMT-3)
           identical to homocysteine S-methyltransferase HMT-3
           [Arabidopsis thaliana] GI:9966515; similar to
           homocysteine S-methyltransferase AtHMT-2 (GI:6685163)
           [Arabidopsis thaliana]; similar to selenocysteine
           methyltransferase GB:P56707 from [Astragalus bisulcatus]
          Length = 347

 Score = 48.8 bits (111), Expect = 3e-06
 Identities = 22/45 (48%), Positives = 29/45 (64%)
 Frame = +2

Query: 95  LALGINCIALENTTAALQHLHGLTVLPLVVYPNSGEHYDAVSKTW 229
           +A+GINC A     A +  L  +T  P+VVYPNSGE YD ++K W
Sbjct: 245 VAIGINCTAPRYIHALIISLRQMTRKPIVVYPNSGEVYDGLNKKW 289



 Score = 41.5 bits (93), Expect = 4e-04
 Identities = 16/27 (59%), Positives = 20/27 (74%)
 Frame = +3

Query: 264 YLPQWQAAGARLIGGCCRTTPADIAAL 344
           Y+ +W+ AGA L GGCCRTTP  I A+
Sbjct: 303 YVSKWRDAGASLFGGCCRTTPNTIRAI 329


>At3g63250.2 68416.m07106 homocysteine S-methyltransferase 2 (HMT-2)
           99.7% identical to homocysteine S-methyltransferase
           AtHMT-2 (GI:6685163) [Arabidopsis thaliana]
          Length = 293

 Score = 43.2 bits (97), Expect = 1e-04
 Identities = 19/45 (42%), Positives = 26/45 (57%)
 Frame = +2

Query: 95  LALGINCIALENTTAALQHLHGLTVLPLVVYPNSGEHYDAVSKTW 229
           +A+GINC         +  +  +T  P++VYPNSGE YDA  K W
Sbjct: 199 VAVGINCTPPRFIEGLVLEIEKVTSKPILVYPNSGESYDADRKEW 243



 Score = 42.3 bits (95), Expect = 2e-04
 Identities = 16/30 (53%), Positives = 20/30 (66%)
 Frame = +3

Query: 264 YLPQWQAAGARLIGGCCRTTPADIAALKAR 353
           Y+ +W  AG  L+GGCCRTTP  I A+  R
Sbjct: 257 YVEKWMDAGVSLLGGCCRTTPTTIRAIHKR 286


>At3g63250.1 68416.m07107 homocysteine S-methyltransferase 2 (HMT-2)
           99.7% identical to homocysteine S-methyltransferase
           AtHMT-2 (GI:6685163) [Arabidopsis thaliana]
          Length = 333

 Score = 43.2 bits (97), Expect = 1e-04
 Identities = 19/45 (42%), Positives = 26/45 (57%)
 Frame = +2

Query: 95  LALGINCIALENTTAALQHLHGLTVLPLVVYPNSGEHYDAVSKTW 229
           +A+GINC         +  +  +T  P++VYPNSGE YDA  K W
Sbjct: 239 VAVGINCTPPRFIEGLVLEIEKVTSKPILVYPNSGESYDADRKEW 283



 Score = 42.3 bits (95), Expect = 2e-04
 Identities = 16/30 (53%), Positives = 20/30 (66%)
 Frame = +3

Query: 264 YLPQWQAAGARLIGGCCRTTPADIAALKAR 353
           Y+ +W  AG  L+GGCCRTTP  I A+  R
Sbjct: 297 YVEKWMDAGVSLLGGCCRTTPTTIRAIHKR 326


>At3g25900.1 68416.m03228 homocysteine S-methyltransferase 1 (HMT-1)
           identical to GB:AAF23821 from [Arabidopsis thaliana]
          Length = 326

 Score = 40.7 bits (91), Expect = 7e-04
 Identities = 15/27 (55%), Positives = 21/27 (77%)
 Frame = +3

Query: 264 YLPQWQAAGARLIGGCCRTTPADIAAL 344
           +  +W+  GA+LIGGCCRTTP+ I A+
Sbjct: 293 FATKWRDLGAKLIGGCCRTTPSTINAI 319



 Score = 38.3 bits (85), Expect = 0.004
 Identities = 18/44 (40%), Positives = 25/44 (56%)
 Frame = +2

Query: 98  ALGINCIALENTTAALQHLHGLTVLPLVVYPNSGEHYDAVSKTW 229
           A+GINC   +     ++    LT   +VVYPNSGE +D  +K W
Sbjct: 236 AVGINCAPPQFIENLIRKFAKLTKKAIVVYPNSGEVWDGKAKQW 279


>At3g53720.1 68416.m05934 cation/hydrogen exchanger, putative
           (CHX20) monovalent cation:proton antiporter family 2
           (CPA2) member, PMID:11500563
          Length = 842

 Score = 27.9 bits (59), Expect = 5.2
 Identities = 12/33 (36%), Positives = 16/33 (48%)
 Frame = +2

Query: 230 HHHGEHCAXGGLSAAVAGRWRTVDWRVLSHHAC 328
           HHH +    G +   V   WR V+ RVL +  C
Sbjct: 590 HHHQDGGGDGNVPENVGHGWRLVNQRVLKNAPC 622


>At3g52330.1 68416.m05751 F-box family protein-related contains weak
           hit to TIGRFAM TIGR01640 : F-box protein interaction
           domain;  similar to F-box protein family, AtFBX8
           (GI:20197464) [Arabidopsis thaliana]
          Length = 307

 Score = 27.5 bits (58), Expect = 6.8
 Identities = 17/55 (30%), Positives = 27/55 (49%), Gaps = 1/55 (1%)
 Frame = +2

Query: 161 LTVLPLVVYPNSGEHYDAVSKTWHHHGEHCAXGGLSAAVAGRWR-TVDWRVLSHH 322
           L  LP+++ P  GE+Y    K W++ G H         ++  WR   +W+V S H
Sbjct: 122 LVDLPIILTPKGGENY----KIWYYLG-HDPVHDEYKVLSFIWRHNKEWKVRSEH 171


>At1g30825.1 68414.m03769 actin-related protein 2/3 complex 34kDa
           subunit family / arp2/3 complex 34kDa subunit family
           contains Pfam PF04045: Arp2/3 complex, 34kD subunit
           p34-Arc; similar to ARP2/3 complex 34 kDa subunit
           (P34-ARC) (Swiss-Prot:O96623) [Dictyostelium
           discoideum]; similar to ARP2/3 complex 34 kDa subunit
           (P34-ARC) (Actin-related protein 2/3 complex subunit 2)
           (Swiss-Prot:O15144) [Homo sapiens]
          Length = 318

 Score = 27.1 bits (57), Expect = 9.0
 Identities = 16/63 (25%), Positives = 25/63 (39%)
 Frame = +2

Query: 371 STSFIVGLISAXNXALPXGGTSALSGPTKPQNVMPHPPEQTCRTXGFKIILLADVRLQTR 550
           +TSF+   + A   A      S    PT PQ +   P E      GF   ++    ++ +
Sbjct: 186 ATSFLKEFVEARRAAALNTAPSCSWSPTAPQELEGAPKETLSANAGFVTFVIFPRHVEGK 245

Query: 551 SLD 559
            LD
Sbjct: 246 KLD 248


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,017,513
Number of Sequences: 28952
Number of extensions: 266657
Number of successful extensions: 791
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 762
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 791
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1131744440
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)