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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ps4M0637.Seq
         (907 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g16520.2 68417.m02501 autophagy 8f (APG8f) identical to autop...   101   9e-22
At4g16520.1 68417.m02500 autophagy 8f (APG8f) identical to autop...   101   9e-22
At2g05630.1 68415.m00599 autophagy 8d (APG8d) identical to autop...   101   9e-22
At2g45170.2 68415.m05624 autophagy 8e (APG8e) identical to autop...    99   5e-21
At2g45170.1 68415.m05623 autophagy 8e (APG8e) identical to autop...    99   5e-21
At3g60640.1 68416.m06785 autophagy 8g (APG8g) identical to autop...    97   1e-20
At1g62040.1 68414.m06997 autophagy 8c (APG8c) identical to autop...    92   5e-19
At4g21980.1 68417.m03182 autophagy 8a (APG8a) identical to autop...    89   4e-18
At4g04620.2 68417.m00676 autophagy 8b (APG8b) identical to autop...    87   1e-17
At4g04620.1 68417.m00675 autophagy 8b (APG8b) identical to autop...    87   1e-17
At3g15580.1 68416.m01974 autophagy 8i (APG8i) identical to autop...    76   4e-14
At3g06420.1 68416.m00740 autophagy 8h (APG8h) identical to autop...    73   2e-13
At5g06680.1 68418.m00754 tubulin family protein similar to SP|Q9...    30   1.8  
At1g02570.1 68414.m00208 expressed protein                             29   4.2  
At3g01015.1 68416.m00001 expressed protein ; expression supporte...    29   5.6  
At5g03140.1 68418.m00262 lectin protein kinase family protein co...    28   7.4  

>At4g16520.2 68417.m02501 autophagy 8f (APG8f) identical to
           autophagy 8f [Arabidopsis thaliana] GI:19912161;
           contains Pfam profile PF02991: Microtubule associated
           protein 1A/1B, light chain 3
          Length = 121

 Score =  101 bits (241), Expect = 9e-22
 Identities = 46/72 (63%), Positives = 56/72 (77%)
 Frame = +2

Query: 35  KFQYKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKVALGDLDKKKYLVPSDLTVGQFY 214
           K  +K+EH  EKR+AE  +IR KYPDR+PVIVEKA K  +  +DKKKYLVP+DLTVGQF 
Sbjct: 3   KSSFKQEHDLEKRRAEAARIREKYPDRIPVIVEKAEKSDIPTIDKKKYLVPADLTVGQFV 62

Query: 215 FLIRKRIHLRPE 250
           ++IRKRI L  E
Sbjct: 63  YVIRKRIKLSAE 74



 Score = 56.0 bits (129), Expect = 3e-08
 Identities = 22/46 (47%), Positives = 33/46 (71%)
 Frame = +1

Query: 241 AA*RRLFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 378
           +A + +F FV+NV+PP  A M S+Y+E  D+D FLY+ +S EN +G
Sbjct: 72  SAEKAIFIFVDNVLPPAGALMSSVYEEKKDDDGFLYVTYSGENTFG 117


>At4g16520.1 68417.m02500 autophagy 8f (APG8f) identical to
           autophagy 8f [Arabidopsis thaliana] GI:19912161;
           contains Pfam profile PF02991: Microtubule associated
           protein 1A/1B, light chain 3
          Length = 121

 Score =  101 bits (241), Expect = 9e-22
 Identities = 46/72 (63%), Positives = 56/72 (77%)
 Frame = +2

Query: 35  KFQYKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKVALGDLDKKKYLVPSDLTVGQFY 214
           K  +K+EH  EKR+AE  +IR KYPDR+PVIVEKA K  +  +DKKKYLVP+DLTVGQF 
Sbjct: 3   KSSFKQEHDLEKRRAEAARIREKYPDRIPVIVEKAEKSDIPTIDKKKYLVPADLTVGQFV 62

Query: 215 FLIRKRIHLRPE 250
           ++IRKRI L  E
Sbjct: 63  YVIRKRIKLSAE 74



 Score = 56.0 bits (129), Expect = 3e-08
 Identities = 22/46 (47%), Positives = 33/46 (71%)
 Frame = +1

Query: 241 AA*RRLFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 378
           +A + +F FV+NV+PP  A M S+Y+E  D+D FLY+ +S EN +G
Sbjct: 72  SAEKAIFIFVDNVLPPAGALMSSVYEEKKDDDGFLYVTYSGENTFG 117


>At2g05630.1 68415.m00599 autophagy 8d (APG8d) identical to
           autophagy 8d [Arabidopsis thaliana] GI:19912157;
           contains Pfam profile PF02991: Microtubule associated
           protein 1A/1B, light chain 3
          Length = 120

 Score =  101 bits (241), Expect = 9e-22
 Identities = 44/69 (63%), Positives = 56/69 (81%)
 Frame = +2

Query: 44  YKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKVALGDLDKKKYLVPSDLTVGQFYFLI 223
           +K EH  EKR+AE  +IR KYPDR+PVIVE+A K  + D+D+KKYLVP+DLTVGQF +++
Sbjct: 6   FKHEHPLEKRQAEAARIREKYPDRIPVIVERAEKSDVPDIDRKKYLVPADLTVGQFVYVV 65

Query: 224 RKRIHLRPE 250
           RKRI L PE
Sbjct: 66  RKRIKLSPE 74



 Score = 60.9 bits (141), Expect = 1e-09
 Identities = 22/43 (51%), Positives = 34/43 (79%)
 Frame = +1

Query: 250 RRLFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 378
           + +F FV N++PPT+A M ++Y+EH DED FLY+++S EN +G
Sbjct: 75  KAIFIFVKNILPPTAAIMSAIYEEHKDEDGFLYMSYSGENTFG 117


>At2g45170.2 68415.m05624 autophagy 8e (APG8e) identical to
           autophagy 8e [Arabidopsis thaliana] GI:19912159;
           contains Pfam profile PF02991: Microtubule associated
           protein 1A/1B, light chain 3
          Length = 122

 Score = 98.7 bits (235), Expect = 5e-21
 Identities = 46/69 (66%), Positives = 55/69 (79%)
 Frame = +2

Query: 44  YKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKVALGDLDKKKYLVPSDLTVGQFYFLI 223
           +K +  FEKRKAE  +IR KYPDR+PVIVEKA K  + ++DKKKYLVPSDLTVGQF ++I
Sbjct: 7   FKMDDDFEKRKAEAGRIREKYPDRIPVIVEKAEKSEVPNIDKKKYLVPSDLTVGQFVYVI 66

Query: 224 RKRIHLRPE 250
           RKRI L  E
Sbjct: 67  RKRIKLSAE 75



 Score = 56.4 bits (130), Expect = 2e-08
 Identities = 23/46 (50%), Positives = 33/46 (71%)
 Frame = +1

Query: 241 AA*RRLFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 378
           +A + +F FV+NV+PPT   M S+Y++  DED FLYI +S EN +G
Sbjct: 73  SAEKAIFIFVDNVLPPTGELMSSVYEDKKDEDGFLYITYSGENTFG 118


>At2g45170.1 68415.m05623 autophagy 8e (APG8e) identical to
           autophagy 8e [Arabidopsis thaliana] GI:19912159;
           contains Pfam profile PF02991: Microtubule associated
           protein 1A/1B, light chain 3
          Length = 122

 Score = 98.7 bits (235), Expect = 5e-21
 Identities = 46/69 (66%), Positives = 55/69 (79%)
 Frame = +2

Query: 44  YKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKVALGDLDKKKYLVPSDLTVGQFYFLI 223
           +K +  FEKRKAE  +IR KYPDR+PVIVEKA K  + ++DKKKYLVPSDLTVGQF ++I
Sbjct: 7   FKMDDDFEKRKAEAGRIREKYPDRIPVIVEKAEKSEVPNIDKKKYLVPSDLTVGQFVYVI 66

Query: 224 RKRIHLRPE 250
           RKRI L  E
Sbjct: 67  RKRIKLSAE 75



 Score = 56.4 bits (130), Expect = 2e-08
 Identities = 23/46 (50%), Positives = 33/46 (71%)
 Frame = +1

Query: 241 AA*RRLFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 378
           +A + +F FV+NV+PPT   M S+Y++  DED FLYI +S EN +G
Sbjct: 73  SAEKAIFIFVDNVLPPTGELMSSVYEDKKDEDGFLYITYSGENTFG 118


>At3g60640.1 68416.m06785 autophagy 8g (APG8g) identical to
           autophagy 8g [Arabidopsis thaliana] GI:19912163;
           contains Pfam profile PF02991: Microtubule associated
           protein 1A/1B, light chain 3; supporting cDNA
           gi|19912162|dbj|AB073181.1|
          Length = 121

 Score = 97.5 bits (232), Expect = 1e-20
 Identities = 44/69 (63%), Positives = 56/69 (81%)
 Frame = +2

Query: 44  YKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKVALGDLDKKKYLVPSDLTVGQFYFLI 223
           ++++H FEKRKAE  +IR KY DRVPVIVEK+ K  + ++DKKKYLVP+DLTVGQF ++I
Sbjct: 6   FRQDHDFEKRKAEALRIREKYSDRVPVIVEKSEKSDIPNIDKKKYLVPADLTVGQFVYVI 65

Query: 224 RKRIHLRPE 250
           RKRI L  E
Sbjct: 66  RKRIQLSAE 74



 Score = 57.2 bits (132), Expect = 1e-08
 Identities = 22/47 (46%), Positives = 35/47 (74%)
 Frame = +1

Query: 241 AA*RRLFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYGN 381
           +A + +F FV+NV+PPT A M ++Y E+ +ED FLY+ +S EN +G+
Sbjct: 72  SAEKAIFIFVDNVLPPTGAMMSTIYDENKEEDGFLYVTYSGENTFGS 118


>At1g62040.1 68414.m06997 autophagy 8c (APG8c) identical to
           autophagy 8c [Arabidopsis thaliana] GI:19912155;
           contains Pfam profile PF02991: Microtubule associated
           protein 1A/1B, light chain 3
          Length = 119

 Score = 91.9 bits (218), Expect = 5e-19
 Identities = 40/69 (57%), Positives = 54/69 (78%)
 Frame = +2

Query: 44  YKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKVALGDLDKKKYLVPSDLTVGQFYFLI 223
           +K EH  E+R+ E  +IR KYPDR+PVIVE+A +  + ++DKKKYLVP+DLTVGQF +++
Sbjct: 6   FKLEHPLERRQIESSRIREKYPDRIPVIVERAERSDVPNIDKKKYLVPADLTVGQFVYVV 65

Query: 224 RKRIHLRPE 250
           RKRI L  E
Sbjct: 66  RKRIKLSAE 74



 Score = 55.6 bits (128), Expect = 4e-08
 Identities = 22/46 (47%), Positives = 33/46 (71%)
 Frame = +1

Query: 241 AA*RRLFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 378
           +A + +F FV N +PPT+A M ++Y E+ DED FLY+ +S EN +G
Sbjct: 72  SAEKAIFVFVKNTLPPTAAMMSAIYDENKDEDGFLYMTYSGENTFG 117


>At4g21980.1 68417.m03182 autophagy 8a (APG8a) identical to
           autophagy 8a [Arabidopsis thaliana] GI:19912151;
           contains Pfam profile PF02991: Microtubule associated
           protein 1A/1B, light chain 3
          Length = 122

 Score = 89.0 bits (211), Expect = 4e-18
 Identities = 41/72 (56%), Positives = 53/72 (73%)
 Frame = +2

Query: 35  KFQYKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKVALGDLDKKKYLVPSDLTVGQFY 214
           K  +K  +  E R +E  +IR KYPDR+PVIVEKA +  + D+DKKKYLVP+DLTVGQF 
Sbjct: 3   KSSFKISNPLEARMSESSRIREKYPDRIPVIVEKAGQSDVPDIDKKKYLVPADLTVGQFV 62

Query: 215 FLIRKRIHLRPE 250
           +++RKRI L  E
Sbjct: 63  YVVRKRIKLGAE 74



 Score = 59.3 bits (137), Expect = 3e-09
 Identities = 22/44 (50%), Positives = 33/44 (75%)
 Frame = +1

Query: 250 RRLFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYGN 381
           + +F FV N +PPT+A M ++Y+EH DED FLY+ +S EN +G+
Sbjct: 75  KAIFVFVKNTLPPTAALMSAIYEEHKDEDGFLYMTYSGENTFGS 118


>At4g04620.2 68417.m00676 autophagy 8b (APG8b) identical to
           autophagy 8b [Arabidopsis thaliana] GI:19912153;
           contains Pfam profile PF02991: Microtubule associated
           protein 1A/1B, light chain 3
          Length = 122

 Score = 87.4 bits (207), Expect = 1e-17
 Identities = 41/72 (56%), Positives = 53/72 (73%)
 Frame = +2

Query: 35  KFQYKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKVALGDLDKKKYLVPSDLTVGQFY 214
           K  +K  +  E R AE  +IR KYP+RVPVIVEKA +  + D+DKKKYLVP+DLT+GQF 
Sbjct: 3   KNSFKLSNPLEMRMAESTRIRAKYPERVPVIVEKAGQSDVPDIDKKKYLVPADLTIGQFV 62

Query: 215 FLIRKRIHLRPE 250
           +++RKRI L  E
Sbjct: 63  YVVRKRIKLGAE 74



 Score = 59.7 bits (138), Expect = 3e-09
 Identities = 23/46 (50%), Positives = 33/46 (71%)
 Frame = +1

Query: 250 RRLFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYGN*F 387
           + +F FV N +PPT+A M ++Y+EH DED FLY+ +S EN +G  F
Sbjct: 75  KAIFVFVKNTLPPTAALMSAIYEEHKDEDGFLYMTYSGENTFGGSF 120


>At4g04620.1 68417.m00675 autophagy 8b (APG8b) identical to
           autophagy 8b [Arabidopsis thaliana] GI:19912153;
           contains Pfam profile PF02991: Microtubule associated
           protein 1A/1B, light chain 3
          Length = 122

 Score = 87.4 bits (207), Expect = 1e-17
 Identities = 41/72 (56%), Positives = 53/72 (73%)
 Frame = +2

Query: 35  KFQYKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKVALGDLDKKKYLVPSDLTVGQFY 214
           K  +K  +  E R AE  +IR KYP+RVPVIVEKA +  + D+DKKKYLVP+DLT+GQF 
Sbjct: 3   KNSFKLSNPLEMRMAESTRIRAKYPERVPVIVEKAGQSDVPDIDKKKYLVPADLTIGQFV 62

Query: 215 FLIRKRIHLRPE 250
           +++RKRI L  E
Sbjct: 63  YVVRKRIKLGAE 74



 Score = 59.7 bits (138), Expect = 3e-09
 Identities = 23/46 (50%), Positives = 33/46 (71%)
 Frame = +1

Query: 250 RRLFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYGN*F 387
           + +F FV N +PPT+A M ++Y+EH DED FLY+ +S EN +G  F
Sbjct: 75  KAIFVFVKNTLPPTAALMSAIYEEHKDEDGFLYMTYSGENTFGGSF 120


>At3g15580.1 68416.m01974 autophagy 8i (APG8i) identical to
           autophagy 8i [Arabidopsis thaliana] GI:19912167;
           contains Pfam profile PF02991: Microtubule associated
           protein 1A/1B, light chain 3; supporting cDNA
           gi|21636957|gb|AF492760.1|
          Length = 115

 Score = 75.8 bits (178), Expect = 4e-14
 Identities = 32/68 (47%), Positives = 49/68 (72%)
 Frame = +2

Query: 44  YKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKVALGDLDKKKYLVPSDLTVGQFYFLI 223
           +KE+++ ++R AE  +I  KYP R+PVI EK  K  L  ++KKK+LVP D++VGQF +++
Sbjct: 4   FKEQYTLDERLAESREIIAKYPTRIPVIAEKYCKTDLPAIEKKKFLVPRDMSVGQFIYIL 63

Query: 224 RKRIHLRP 247
             R+HL P
Sbjct: 64  SARLHLSP 71



 Score = 50.4 bits (115), Expect = 2e-06
 Identities = 19/43 (44%), Positives = 30/43 (69%)
 Frame = +1

Query: 250 RRLFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 378
           + LF FVNN +P T+A M S+Y+ + D+D F+Y+ +S E  +G
Sbjct: 73  KALFVFVNNTLPQTAALMDSVYESYKDDDGFVYMCYSSEKTFG 115


>At3g06420.1 68416.m00740 autophagy 8h (APG8h) identical to
           autophagy 8h [Arabidopsis thaliana] GI:19912165;
           contains Pfam profile PF02991: Microtubule associated
           protein 1A/1B, light chain 3; supporting cDNA
           gi|19912164|dbj|AB073182.1|
          Length = 119

 Score = 73.3 bits (172), Expect = 2e-13
 Identities = 32/68 (47%), Positives = 45/68 (66%)
 Frame = +2

Query: 44  YKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKVALGDLDKKKYLVPSDLTVGQFYFLI 223
           +K++ S ++R  E   I  KYPDR+PVI+EK     L D++K KYLVP D+TVG F  ++
Sbjct: 8   FKDQFSSDERLKESNNIIAKYPDRIPVIIEKYSNADLPDMEKNKYLVPRDMTVGHFIHML 67

Query: 224 RKRIHLRP 247
            KR+ L P
Sbjct: 68  SKRMQLDP 75



 Score = 46.4 bits (105), Expect = 3e-05
 Identities = 19/43 (44%), Positives = 28/43 (65%)
 Frame = +1

Query: 250 RRLFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 378
           + LF FV+N +P T++ M SLY    +ED FLY+ +S E  +G
Sbjct: 77  KALFVFVHNTLPQTASRMDSLYNTFKEEDGFLYMCYSTEKTFG 119


>At5g06680.1 68418.m00754 tubulin family protein similar to
           SP|Q96CW5 Gamma-tubulin complex component 3 {Homo
           sapiens}
          Length = 838

 Score = 30.3 bits (65), Expect = 1.8
 Identities = 15/37 (40%), Positives = 19/37 (51%), Gaps = 1/37 (2%)
 Frame = +3

Query: 372 LWKLI-PNYSLSNSFTLSDHMVKFNLSLRLKLCIILW 479
           +WK + PN   SNSF      VK  L   L+ C +LW
Sbjct: 622 IWKTMKPNCITSNSFVKLQSSVKLQLLSALRRCQVLW 658


>At1g02570.1 68414.m00208 expressed protein
          Length = 431

 Score = 29.1 bits (62), Expect = 4.2
 Identities = 17/41 (41%), Positives = 23/41 (56%)
 Frame = +3

Query: 453 RLKLCIILWYFFIINWHIIYICLHMNSIKLFKQVKDCFVFT 575
           RL L I L     +NW + YIC+ +  +K+FK   D FV T
Sbjct: 366 RLNLPIRLEGLNFVNWCMYYICVGIGLMKIFKGFLD-FVNT 405


>At3g01015.1 68416.m00001 expressed protein ; expression supported
           by MPSS
          Length = 488

 Score = 28.7 bits (61), Expect = 5.6
 Identities = 14/32 (43%), Positives = 20/32 (62%)
 Frame = +2

Query: 26  INMKFQYKEEHSFEKRKAEGEKIRRKYPDRVP 121
           IN+  QYK E   +++ AE E+IRR   + VP
Sbjct: 396 INLVEQYKTERERQQKLAEEEEIRRLRKELVP 427


>At5g03140.1 68418.m00262 lectin protein kinase family protein
           contains Pfam domains, PF00138: Legume lectins alpha
           domain, PF00139: Legume lectins beta domain and PF00069:
           Protein kinase domain
          Length = 711

 Score = 28.3 bits (60), Expect = 7.4
 Identities = 20/74 (27%), Positives = 33/74 (44%), Gaps = 4/74 (5%)
 Frame = +1

Query: 205 PVLLLDPETYSPAA*RRLFFFVNNVIPPTSATMGSLYQEHHDEDFF----LYIAFSDENV 372
           PV    PET SPA+    F F    + P+S   G  +    DED+      ++  ++E  
Sbjct: 81  PVKFRHPETKSPASFTTYFSFSVTNLNPSSIGGGLAFVISPDEDYLGSTGGFLGLTEETG 140

Query: 373 YGN*FQIIHFQIVL 414
            G+ F  + F  ++
Sbjct: 141 SGSGFVAVEFDTLM 154


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,543,582
Number of Sequences: 28952
Number of extensions: 401681
Number of successful extensions: 1048
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1009
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1046
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2139598560
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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