BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ps4M0637.Seq (907 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g16520.2 68417.m02501 autophagy 8f (APG8f) identical to autop... 101 9e-22 At4g16520.1 68417.m02500 autophagy 8f (APG8f) identical to autop... 101 9e-22 At2g05630.1 68415.m00599 autophagy 8d (APG8d) identical to autop... 101 9e-22 At2g45170.2 68415.m05624 autophagy 8e (APG8e) identical to autop... 99 5e-21 At2g45170.1 68415.m05623 autophagy 8e (APG8e) identical to autop... 99 5e-21 At3g60640.1 68416.m06785 autophagy 8g (APG8g) identical to autop... 97 1e-20 At1g62040.1 68414.m06997 autophagy 8c (APG8c) identical to autop... 92 5e-19 At4g21980.1 68417.m03182 autophagy 8a (APG8a) identical to autop... 89 4e-18 At4g04620.2 68417.m00676 autophagy 8b (APG8b) identical to autop... 87 1e-17 At4g04620.1 68417.m00675 autophagy 8b (APG8b) identical to autop... 87 1e-17 At3g15580.1 68416.m01974 autophagy 8i (APG8i) identical to autop... 76 4e-14 At3g06420.1 68416.m00740 autophagy 8h (APG8h) identical to autop... 73 2e-13 At5g06680.1 68418.m00754 tubulin family protein similar to SP|Q9... 30 1.8 At1g02570.1 68414.m00208 expressed protein 29 4.2 At3g01015.1 68416.m00001 expressed protein ; expression supporte... 29 5.6 At5g03140.1 68418.m00262 lectin protein kinase family protein co... 28 7.4 >At4g16520.2 68417.m02501 autophagy 8f (APG8f) identical to autophagy 8f [Arabidopsis thaliana] GI:19912161; contains Pfam profile PF02991: Microtubule associated protein 1A/1B, light chain 3 Length = 121 Score = 101 bits (241), Expect = 9e-22 Identities = 46/72 (63%), Positives = 56/72 (77%) Frame = +2 Query: 35 KFQYKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKVALGDLDKKKYLVPSDLTVGQFY 214 K +K+EH EKR+AE +IR KYPDR+PVIVEKA K + +DKKKYLVP+DLTVGQF Sbjct: 3 KSSFKQEHDLEKRRAEAARIREKYPDRIPVIVEKAEKSDIPTIDKKKYLVPADLTVGQFV 62 Query: 215 FLIRKRIHLRPE 250 ++IRKRI L E Sbjct: 63 YVIRKRIKLSAE 74 Score = 56.0 bits (129), Expect = 3e-08 Identities = 22/46 (47%), Positives = 33/46 (71%) Frame = +1 Query: 241 AA*RRLFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 378 +A + +F FV+NV+PP A M S+Y+E D+D FLY+ +S EN +G Sbjct: 72 SAEKAIFIFVDNVLPPAGALMSSVYEEKKDDDGFLYVTYSGENTFG 117 >At4g16520.1 68417.m02500 autophagy 8f (APG8f) identical to autophagy 8f [Arabidopsis thaliana] GI:19912161; contains Pfam profile PF02991: Microtubule associated protein 1A/1B, light chain 3 Length = 121 Score = 101 bits (241), Expect = 9e-22 Identities = 46/72 (63%), Positives = 56/72 (77%) Frame = +2 Query: 35 KFQYKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKVALGDLDKKKYLVPSDLTVGQFY 214 K +K+EH EKR+AE +IR KYPDR+PVIVEKA K + +DKKKYLVP+DLTVGQF Sbjct: 3 KSSFKQEHDLEKRRAEAARIREKYPDRIPVIVEKAEKSDIPTIDKKKYLVPADLTVGQFV 62 Query: 215 FLIRKRIHLRPE 250 ++IRKRI L E Sbjct: 63 YVIRKRIKLSAE 74 Score = 56.0 bits (129), Expect = 3e-08 Identities = 22/46 (47%), Positives = 33/46 (71%) Frame = +1 Query: 241 AA*RRLFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 378 +A + +F FV+NV+PP A M S+Y+E D+D FLY+ +S EN +G Sbjct: 72 SAEKAIFIFVDNVLPPAGALMSSVYEEKKDDDGFLYVTYSGENTFG 117 >At2g05630.1 68415.m00599 autophagy 8d (APG8d) identical to autophagy 8d [Arabidopsis thaliana] GI:19912157; contains Pfam profile PF02991: Microtubule associated protein 1A/1B, light chain 3 Length = 120 Score = 101 bits (241), Expect = 9e-22 Identities = 44/69 (63%), Positives = 56/69 (81%) Frame = +2 Query: 44 YKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKVALGDLDKKKYLVPSDLTVGQFYFLI 223 +K EH EKR+AE +IR KYPDR+PVIVE+A K + D+D+KKYLVP+DLTVGQF +++ Sbjct: 6 FKHEHPLEKRQAEAARIREKYPDRIPVIVERAEKSDVPDIDRKKYLVPADLTVGQFVYVV 65 Query: 224 RKRIHLRPE 250 RKRI L PE Sbjct: 66 RKRIKLSPE 74 Score = 60.9 bits (141), Expect = 1e-09 Identities = 22/43 (51%), Positives = 34/43 (79%) Frame = +1 Query: 250 RRLFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 378 + +F FV N++PPT+A M ++Y+EH DED FLY+++S EN +G Sbjct: 75 KAIFIFVKNILPPTAAIMSAIYEEHKDEDGFLYMSYSGENTFG 117 >At2g45170.2 68415.m05624 autophagy 8e (APG8e) identical to autophagy 8e [Arabidopsis thaliana] GI:19912159; contains Pfam profile PF02991: Microtubule associated protein 1A/1B, light chain 3 Length = 122 Score = 98.7 bits (235), Expect = 5e-21 Identities = 46/69 (66%), Positives = 55/69 (79%) Frame = +2 Query: 44 YKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKVALGDLDKKKYLVPSDLTVGQFYFLI 223 +K + FEKRKAE +IR KYPDR+PVIVEKA K + ++DKKKYLVPSDLTVGQF ++I Sbjct: 7 FKMDDDFEKRKAEAGRIREKYPDRIPVIVEKAEKSEVPNIDKKKYLVPSDLTVGQFVYVI 66 Query: 224 RKRIHLRPE 250 RKRI L E Sbjct: 67 RKRIKLSAE 75 Score = 56.4 bits (130), Expect = 2e-08 Identities = 23/46 (50%), Positives = 33/46 (71%) Frame = +1 Query: 241 AA*RRLFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 378 +A + +F FV+NV+PPT M S+Y++ DED FLYI +S EN +G Sbjct: 73 SAEKAIFIFVDNVLPPTGELMSSVYEDKKDEDGFLYITYSGENTFG 118 >At2g45170.1 68415.m05623 autophagy 8e (APG8e) identical to autophagy 8e [Arabidopsis thaliana] GI:19912159; contains Pfam profile PF02991: Microtubule associated protein 1A/1B, light chain 3 Length = 122 Score = 98.7 bits (235), Expect = 5e-21 Identities = 46/69 (66%), Positives = 55/69 (79%) Frame = +2 Query: 44 YKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKVALGDLDKKKYLVPSDLTVGQFYFLI 223 +K + FEKRKAE +IR KYPDR+PVIVEKA K + ++DKKKYLVPSDLTVGQF ++I Sbjct: 7 FKMDDDFEKRKAEAGRIREKYPDRIPVIVEKAEKSEVPNIDKKKYLVPSDLTVGQFVYVI 66 Query: 224 RKRIHLRPE 250 RKRI L E Sbjct: 67 RKRIKLSAE 75 Score = 56.4 bits (130), Expect = 2e-08 Identities = 23/46 (50%), Positives = 33/46 (71%) Frame = +1 Query: 241 AA*RRLFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 378 +A + +F FV+NV+PPT M S+Y++ DED FLYI +S EN +G Sbjct: 73 SAEKAIFIFVDNVLPPTGELMSSVYEDKKDEDGFLYITYSGENTFG 118 >At3g60640.1 68416.m06785 autophagy 8g (APG8g) identical to autophagy 8g [Arabidopsis thaliana] GI:19912163; contains Pfam profile PF02991: Microtubule associated protein 1A/1B, light chain 3; supporting cDNA gi|19912162|dbj|AB073181.1| Length = 121 Score = 97.5 bits (232), Expect = 1e-20 Identities = 44/69 (63%), Positives = 56/69 (81%) Frame = +2 Query: 44 YKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKVALGDLDKKKYLVPSDLTVGQFYFLI 223 ++++H FEKRKAE +IR KY DRVPVIVEK+ K + ++DKKKYLVP+DLTVGQF ++I Sbjct: 6 FRQDHDFEKRKAEALRIREKYSDRVPVIVEKSEKSDIPNIDKKKYLVPADLTVGQFVYVI 65 Query: 224 RKRIHLRPE 250 RKRI L E Sbjct: 66 RKRIQLSAE 74 Score = 57.2 bits (132), Expect = 1e-08 Identities = 22/47 (46%), Positives = 35/47 (74%) Frame = +1 Query: 241 AA*RRLFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYGN 381 +A + +F FV+NV+PPT A M ++Y E+ +ED FLY+ +S EN +G+ Sbjct: 72 SAEKAIFIFVDNVLPPTGAMMSTIYDENKEEDGFLYVTYSGENTFGS 118 >At1g62040.1 68414.m06997 autophagy 8c (APG8c) identical to autophagy 8c [Arabidopsis thaliana] GI:19912155; contains Pfam profile PF02991: Microtubule associated protein 1A/1B, light chain 3 Length = 119 Score = 91.9 bits (218), Expect = 5e-19 Identities = 40/69 (57%), Positives = 54/69 (78%) Frame = +2 Query: 44 YKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKVALGDLDKKKYLVPSDLTVGQFYFLI 223 +K EH E+R+ E +IR KYPDR+PVIVE+A + + ++DKKKYLVP+DLTVGQF +++ Sbjct: 6 FKLEHPLERRQIESSRIREKYPDRIPVIVERAERSDVPNIDKKKYLVPADLTVGQFVYVV 65 Query: 224 RKRIHLRPE 250 RKRI L E Sbjct: 66 RKRIKLSAE 74 Score = 55.6 bits (128), Expect = 4e-08 Identities = 22/46 (47%), Positives = 33/46 (71%) Frame = +1 Query: 241 AA*RRLFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 378 +A + +F FV N +PPT+A M ++Y E+ DED FLY+ +S EN +G Sbjct: 72 SAEKAIFVFVKNTLPPTAAMMSAIYDENKDEDGFLYMTYSGENTFG 117 >At4g21980.1 68417.m03182 autophagy 8a (APG8a) identical to autophagy 8a [Arabidopsis thaliana] GI:19912151; contains Pfam profile PF02991: Microtubule associated protein 1A/1B, light chain 3 Length = 122 Score = 89.0 bits (211), Expect = 4e-18 Identities = 41/72 (56%), Positives = 53/72 (73%) Frame = +2 Query: 35 KFQYKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKVALGDLDKKKYLVPSDLTVGQFY 214 K +K + E R +E +IR KYPDR+PVIVEKA + + D+DKKKYLVP+DLTVGQF Sbjct: 3 KSSFKISNPLEARMSESSRIREKYPDRIPVIVEKAGQSDVPDIDKKKYLVPADLTVGQFV 62 Query: 215 FLIRKRIHLRPE 250 +++RKRI L E Sbjct: 63 YVVRKRIKLGAE 74 Score = 59.3 bits (137), Expect = 3e-09 Identities = 22/44 (50%), Positives = 33/44 (75%) Frame = +1 Query: 250 RRLFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYGN 381 + +F FV N +PPT+A M ++Y+EH DED FLY+ +S EN +G+ Sbjct: 75 KAIFVFVKNTLPPTAALMSAIYEEHKDEDGFLYMTYSGENTFGS 118 >At4g04620.2 68417.m00676 autophagy 8b (APG8b) identical to autophagy 8b [Arabidopsis thaliana] GI:19912153; contains Pfam profile PF02991: Microtubule associated protein 1A/1B, light chain 3 Length = 122 Score = 87.4 bits (207), Expect = 1e-17 Identities = 41/72 (56%), Positives = 53/72 (73%) Frame = +2 Query: 35 KFQYKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKVALGDLDKKKYLVPSDLTVGQFY 214 K +K + E R AE +IR KYP+RVPVIVEKA + + D+DKKKYLVP+DLT+GQF Sbjct: 3 KNSFKLSNPLEMRMAESTRIRAKYPERVPVIVEKAGQSDVPDIDKKKYLVPADLTIGQFV 62 Query: 215 FLIRKRIHLRPE 250 +++RKRI L E Sbjct: 63 YVVRKRIKLGAE 74 Score = 59.7 bits (138), Expect = 3e-09 Identities = 23/46 (50%), Positives = 33/46 (71%) Frame = +1 Query: 250 RRLFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYGN*F 387 + +F FV N +PPT+A M ++Y+EH DED FLY+ +S EN +G F Sbjct: 75 KAIFVFVKNTLPPTAALMSAIYEEHKDEDGFLYMTYSGENTFGGSF 120 >At4g04620.1 68417.m00675 autophagy 8b (APG8b) identical to autophagy 8b [Arabidopsis thaliana] GI:19912153; contains Pfam profile PF02991: Microtubule associated protein 1A/1B, light chain 3 Length = 122 Score = 87.4 bits (207), Expect = 1e-17 Identities = 41/72 (56%), Positives = 53/72 (73%) Frame = +2 Query: 35 KFQYKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKVALGDLDKKKYLVPSDLTVGQFY 214 K +K + E R AE +IR KYP+RVPVIVEKA + + D+DKKKYLVP+DLT+GQF Sbjct: 3 KNSFKLSNPLEMRMAESTRIRAKYPERVPVIVEKAGQSDVPDIDKKKYLVPADLTIGQFV 62 Query: 215 FLIRKRIHLRPE 250 +++RKRI L E Sbjct: 63 YVVRKRIKLGAE 74 Score = 59.7 bits (138), Expect = 3e-09 Identities = 23/46 (50%), Positives = 33/46 (71%) Frame = +1 Query: 250 RRLFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYGN*F 387 + +F FV N +PPT+A M ++Y+EH DED FLY+ +S EN +G F Sbjct: 75 KAIFVFVKNTLPPTAALMSAIYEEHKDEDGFLYMTYSGENTFGGSF 120 >At3g15580.1 68416.m01974 autophagy 8i (APG8i) identical to autophagy 8i [Arabidopsis thaliana] GI:19912167; contains Pfam profile PF02991: Microtubule associated protein 1A/1B, light chain 3; supporting cDNA gi|21636957|gb|AF492760.1| Length = 115 Score = 75.8 bits (178), Expect = 4e-14 Identities = 32/68 (47%), Positives = 49/68 (72%) Frame = +2 Query: 44 YKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKVALGDLDKKKYLVPSDLTVGQFYFLI 223 +KE+++ ++R AE +I KYP R+PVI EK K L ++KKK+LVP D++VGQF +++ Sbjct: 4 FKEQYTLDERLAESREIIAKYPTRIPVIAEKYCKTDLPAIEKKKFLVPRDMSVGQFIYIL 63 Query: 224 RKRIHLRP 247 R+HL P Sbjct: 64 SARLHLSP 71 Score = 50.4 bits (115), Expect = 2e-06 Identities = 19/43 (44%), Positives = 30/43 (69%) Frame = +1 Query: 250 RRLFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 378 + LF FVNN +P T+A M S+Y+ + D+D F+Y+ +S E +G Sbjct: 73 KALFVFVNNTLPQTAALMDSVYESYKDDDGFVYMCYSSEKTFG 115 >At3g06420.1 68416.m00740 autophagy 8h (APG8h) identical to autophagy 8h [Arabidopsis thaliana] GI:19912165; contains Pfam profile PF02991: Microtubule associated protein 1A/1B, light chain 3; supporting cDNA gi|19912164|dbj|AB073182.1| Length = 119 Score = 73.3 bits (172), Expect = 2e-13 Identities = 32/68 (47%), Positives = 45/68 (66%) Frame = +2 Query: 44 YKEEHSFEKRKAEGEKIRRKYPDRVPVIVEKAPKVALGDLDKKKYLVPSDLTVGQFYFLI 223 +K++ S ++R E I KYPDR+PVI+EK L D++K KYLVP D+TVG F ++ Sbjct: 8 FKDQFSSDERLKESNNIIAKYPDRIPVIIEKYSNADLPDMEKNKYLVPRDMTVGHFIHML 67 Query: 224 RKRIHLRP 247 KR+ L P Sbjct: 68 SKRMQLDP 75 Score = 46.4 bits (105), Expect = 3e-05 Identities = 19/43 (44%), Positives = 28/43 (65%) Frame = +1 Query: 250 RRLFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 378 + LF FV+N +P T++ M SLY +ED FLY+ +S E +G Sbjct: 77 KALFVFVHNTLPQTASRMDSLYNTFKEEDGFLYMCYSTEKTFG 119 >At5g06680.1 68418.m00754 tubulin family protein similar to SP|Q96CW5 Gamma-tubulin complex component 3 {Homo sapiens} Length = 838 Score = 30.3 bits (65), Expect = 1.8 Identities = 15/37 (40%), Positives = 19/37 (51%), Gaps = 1/37 (2%) Frame = +3 Query: 372 LWKLI-PNYSLSNSFTLSDHMVKFNLSLRLKLCIILW 479 +WK + PN SNSF VK L L+ C +LW Sbjct: 622 IWKTMKPNCITSNSFVKLQSSVKLQLLSALRRCQVLW 658 >At1g02570.1 68414.m00208 expressed protein Length = 431 Score = 29.1 bits (62), Expect = 4.2 Identities = 17/41 (41%), Positives = 23/41 (56%) Frame = +3 Query: 453 RLKLCIILWYFFIINWHIIYICLHMNSIKLFKQVKDCFVFT 575 RL L I L +NW + YIC+ + +K+FK D FV T Sbjct: 366 RLNLPIRLEGLNFVNWCMYYICVGIGLMKIFKGFLD-FVNT 405 >At3g01015.1 68416.m00001 expressed protein ; expression supported by MPSS Length = 488 Score = 28.7 bits (61), Expect = 5.6 Identities = 14/32 (43%), Positives = 20/32 (62%) Frame = +2 Query: 26 INMKFQYKEEHSFEKRKAEGEKIRRKYPDRVP 121 IN+ QYK E +++ AE E+IRR + VP Sbjct: 396 INLVEQYKTERERQQKLAEEEEIRRLRKELVP 427 >At5g03140.1 68418.m00262 lectin protein kinase family protein contains Pfam domains, PF00138: Legume lectins alpha domain, PF00139: Legume lectins beta domain and PF00069: Protein kinase domain Length = 711 Score = 28.3 bits (60), Expect = 7.4 Identities = 20/74 (27%), Positives = 33/74 (44%), Gaps = 4/74 (5%) Frame = +1 Query: 205 PVLLLDPETYSPAA*RRLFFFVNNVIPPTSATMGSLYQEHHDEDFF----LYIAFSDENV 372 PV PET SPA+ F F + P+S G + DED+ ++ ++E Sbjct: 81 PVKFRHPETKSPASFTTYFSFSVTNLNPSSIGGGLAFVISPDEDYLGSTGGFLGLTEETG 140 Query: 373 YGN*FQIIHFQIVL 414 G+ F + F ++ Sbjct: 141 SGSGFVAVEFDTLM 154 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 18,543,582 Number of Sequences: 28952 Number of extensions: 401681 Number of successful extensions: 1048 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 1009 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1046 length of database: 12,070,560 effective HSP length: 81 effective length of database: 9,725,448 effective search space used: 2139598560 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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