Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ps4M0628.Seq
(826 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g68090.1 68414.m07778 annexin 5 (ANN5) identical to calcium-b... 42 4e-04
At5g12380.1 68418.m01456 annexin, putative similar to annexin [F... 40 0.002
At2g38760.1 68415.m04759 annexin 3 (ANN3) nearly identical to an... 37 0.014
At5g65020.1 68418.m08179 annexin 2 (ANN2) identical to annexin (... 35 0.057
At1g35720.1 68414.m04440 annexin 1 (ANN1) identical to annexin (... 34 0.13
At5g10230.1 68418.m01187 annexin 7 (ANN7) nearly identical to ca... 31 0.70
At5g10220.1 68418.m01185 annexin 6 (ANN6) nearly identical to ca... 31 1.2
At3g02420.1 68416.m00229 expressed protein 28 8.7
>At1g68090.1 68414.m07778 annexin 5 (ANN5) identical to
calcium-binding protein annexin 5 [Arabidopsis thaliana]
GI:12667520
Length = 316
Score = 42.3 bits (95), Expect = 4e-04
Identities = 21/74 (28%), Positives = 42/74 (56%), Gaps = 3/74 (4%)
Frame = +1
Query: 256 KDLISELKSELTGNLENVIVALMTPLPH---FYAKELHDAVSGIGTDEEAIIEILCTLSN 426
K+L ++ E GN E+V++ ++ + ++AK L ++ G+GTD+ A+I I+ T +
Sbjct: 215 KELGKAIRDETRGNFEHVLLTILQCAENSCFYFAKALRKSMKGLGTDDTALIRIVVTRAE 274
Query: 427 YGIRTISAFYEQLY 468
++ I Y + Y
Sbjct: 275 VDMQFIITEYRKRY 288
Score = 30.3 bits (65), Expect = 1.6
Identities = 13/25 (52%), Positives = 18/25 (72%)
Frame = +3
Query: 141 LRKAMKGFGTDEKAIIDVLCRRGIV 215
LRK+MKG GTD+ A+I ++ R V
Sbjct: 251 LRKSMKGLGTDDTALIRIVVTRAEV 275
>At5g12380.1 68418.m01456 annexin, putative similar to annexin
[Fragaria x ananassa] GI:6010777, annexin p33 [Zea mays]
GI:6272285; contains Pfam profile PF00191: Annexin
Length = 316
Score = 39.9 bits (89), Expect = 0.002
Identities = 19/69 (27%), Positives = 37/69 (53%)
Frame = +1
Query: 250 LCKDLISELKSELTGNLENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNY 429
+ KDL++ +E L I + P +YAK L ++++ +GTDE+A+ ++ T +
Sbjct: 215 ITKDLLNHPTNEYLSALRAAIRCIKNPT-RYYAKVLRNSINTVGTDEDALNRVIVTRAEK 273
Query: 430 GIRTISAFY 456
+ I+ Y
Sbjct: 274 DLTNITGLY 282
Score = 37.5 bits (83), Expect = 0.011
Identities = 27/89 (30%), Positives = 39/89 (43%)
Frame = +1
Query: 256 KDLISELKSELTGNLENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGI 435
+DLI +LKSEL+GN E I + P A + A+ D + ++EI C S +
Sbjct: 58 EDLIHQLKSELSGNFERAICLWVLDPPERDALLANLALQKPIPDYKVLVEIACMRSPEDM 117
Query: 436 RTISAFYEQLYGQEPGIGLKRRHVGTLSR 522
Y LY L R +G + R
Sbjct: 118 LAARRAYRCLYKHSLEEDLASRTIGDIRR 146
Score = 30.7 bits (66), Expect = 1.2
Identities = 14/39 (35%), Positives = 20/39 (51%)
Frame = +1
Query: 355 LHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYG 471
LHD + G D E I +L T S+ + I Y+ +YG
Sbjct: 174 LHDEILGKAVDHEETIRVLSTRSSMQLSAIFNRYKDIYG 212
Score = 29.9 bits (64), Expect = 2.1
Identities = 22/74 (29%), Positives = 35/74 (47%), Gaps = 4/74 (5%)
Frame = +1
Query: 313 VALMTPLPHFY----AKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGQEP 480
+A + PHF A+ + A G GT+E AII IL + + + I Y+++Y ++
Sbjct: 1 MATIVSPPHFSPVEDAENIKAACQGWGTNENAIISILGHRNLFQRKLIRQAYQEIYHEDL 60
Query: 481 GIGLKRRHVGTLSR 522
LK G R
Sbjct: 61 IHQLKSELSGNFER 74
Score = 27.9 bits (59), Expect = 8.7
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = +3
Query: 141 LRKAMKGFGTDEKAIIDVLCRRGIVQASRSRR 236
++ A +G+GT+E AII +L R + Q R+
Sbjct: 19 IKAACQGWGTNENAIISILGHRNLFQRKLIRQ 50
>At2g38760.1 68415.m04759 annexin 3 (ANN3) nearly identical to
annexin (AnnAt3) [Arabidopsis thaliana] GI:6503082;
contains Pfam profile PF00191: Annexin
Length = 321
Score = 37.1 bits (82), Expect = 0.014
Identities = 18/63 (28%), Positives = 33/63 (52%)
Frame = +1
Query: 280 SELTGNLENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYE 459
++L L+ I + TP HF AK + D++ G GTDE+++ + T + + + Y
Sbjct: 232 ADLRSLLKVAIFCIDTPEKHF-AKVVRDSIEGFGTDEDSLTRAIVTRAEIDLMKVRGEYF 290
Query: 460 QLY 468
+Y
Sbjct: 291 NMY 293
Score = 35.5 bits (78), Expect = 0.043
Identities = 18/36 (50%), Positives = 25/36 (69%)
Frame = +3
Query: 138 TLRKAMKGFGTDEKAIIDVLCRRGIVQASRSRRPSR 245
TL++A++G+GTDEKAII VL +R Q + R R
Sbjct: 19 TLKQAIRGWGTDEKAIIRVLGQRDQSQRRKIRESFR 54
Score = 33.5 bits (73), Expect = 0.17
Identities = 17/50 (34%), Positives = 27/50 (54%)
Frame = +1
Query: 328 PLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGQE 477
P P ++ L A+ G GTDE+AII +L R I + ++YG++
Sbjct: 11 PSPAQDSETLKQAIRGWGTDEKAIIRVLGQRDQSQRRKIRESFREIYGKD 60
>At5g65020.1 68418.m08179 annexin 2 (ANN2) identical to annexin
(AnnAt2) [Arabidopsis thaliana] GI:4959108
Length = 317
Score = 35.1 bits (77), Expect = 0.057
Identities = 19/50 (38%), Positives = 27/50 (54%)
Frame = +1
Query: 328 PLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGQE 477
PLP A++LH A SG GT+E+ II IL + I + Y Y ++
Sbjct: 11 PLPEDDAEQLHKAFSGWGTNEKLIISILAHRNAAQRSLIRSVYAATYNED 60
>At1g35720.1 68414.m04440 annexin 1 (ANN1) identical to annexin
(AnnAt1) [Arabidopsis thaliana] GI:4959106
Length = 317
Score = 33.9 bits (74), Expect = 0.13
Identities = 18/50 (36%), Positives = 26/50 (52%)
Frame = +1
Query: 328 PLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGQE 477
P P A++L A G GT+E+ II IL S + I Y + YG++
Sbjct: 11 PAPSDDAEQLRTAFEGWGTNEDLIISILAHRSAEQRKVIRQAYHETYGED 60
>At5g10230.1 68418.m01187 annexin 7 (ANN7) nearly identical to
calcium-binding protein annexin 7 [Arabidopsis thaliana]
GI:12667522
Length = 316
Score = 31.5 bits (68), Expect = 0.70
Identities = 14/28 (50%), Positives = 18/28 (64%)
Frame = +3
Query: 141 LRKAMKGFGTDEKAIIDVLCRRGIVQAS 224
L KA KG+GT+E+ II +L R Q S
Sbjct: 20 LYKAFKGWGTNERMIISILAHRNATQRS 47
Score = 31.1 bits (67), Expect = 0.93
Identities = 23/70 (32%), Positives = 30/70 (42%)
Frame = +1
Query: 313 VALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGQEPGIGL 492
V PLP A++L+ A G GT+E II IL + I A Y Y ++ L
Sbjct: 6 VPATVPLPEEDAEQLYKAFKGWGTNERMIISILAHRNATQRSFIRAVYAANYNKDLLKEL 65
Query: 493 KRRHVGTLSR 522
R G R
Sbjct: 66 DRELSGDFER 75
>At5g10220.1 68418.m01185 annexin 6 (ANN6) nearly identical to
calcium-binding protein annexin 6 [Arabidopsis thaliana]
GI:12667518
Length = 318
Score = 30.7 bits (66), Expect = 1.2
Identities = 18/50 (36%), Positives = 25/50 (50%)
Frame = +1
Query: 328 PLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGQE 477
PLP +++LH A G GT+E II IL + I A Y Y ++
Sbjct: 11 PLPEEDSEQLHKAFKGWGTNEGMIISILAHRNATQRSFIRAVYAANYNKD 60
Score = 30.7 bits (66), Expect = 1.2
Identities = 14/28 (50%), Positives = 17/28 (60%)
Frame = +3
Query: 141 LRKAMKGFGTDEKAIIDVLCRRGIVQAS 224
L KA KG+GT+E II +L R Q S
Sbjct: 20 LHKAFKGWGTNEGMIISILAHRNATQRS 47
>At3g02420.1 68416.m00229 expressed protein
Length = 348
Score = 27.9 bits (59), Expect = 8.7
Identities = 25/80 (31%), Positives = 34/80 (42%), Gaps = 1/80 (1%)
Frame = +2
Query: 182 YHRRALPPRHRASL-EIAETFKTNYART*LANSRVNSPATWKMSSSH**LPCPTSTLRSS 358
Y L P H AS E+ + FK + + + V P + SSS PT+T SS
Sbjct: 30 YWSNILIPPHMASRPEVVDHFKRKFYQRYIDPDLVVEPMSTSSSSSQ--SARPTATSASS 87
Query: 359 TMLSQELEPTKKPSSRSCAR 418
T S E + +S S R
Sbjct: 88 TASSNANEQVRSRNSGSVPR 107
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,989,361
Number of Sequences: 28952
Number of extensions: 313745
Number of successful extensions: 822
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 785
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 822
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1892353600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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