BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ps4M0603.Seq (725 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g16640.1 68416.m02127 translationally controlled tumor family... 57 1e-08 At3g05540.1 68416.m00607 translationally controlled tumor family... 56 2e-08 At2g17050.1 68415.m01968 disease resistance protein (TIR-NBS-LRR... 33 0.15 At4g29060.1 68417.m04157 elongation factor Ts family protein sim... 32 0.45 At5g51800.1 68418.m06423 expressed protein 30 1.8 At5g49030.1 68418.m06067 tRNA synthetase class I (I, L, M and V)... 27 9.6 At1g78390.1 68414.m09135 9-cis-epoxycarotenoid dioxygenase, puta... 27 9.6 >At3g16640.1 68416.m02127 translationally controlled tumor family protein similar to translationally controlled tumor protein GB:AAD10032 from [Hevea brasiliensis] Length = 168 Score = 56.8 bits (131), Expect = 1e-08 Identities = 36/88 (40%), Positives = 46/88 (52%) Frame = -1 Query: 515 GHDSAVESGVDIVLNHRLVETYAFGDKKSYTLYLKDYMKKLVAKLEEKAPDQVEVFKTNM 336 G D + + VDIV RL E + DKK + Y+K Y+K L KL E+ DQ VFK + Sbjct: 59 GVDDSTQKVVDIVDTFRLQEQPTY-DKKGFIAYIKKYIKLLTPKLSEE--DQA-VFKKGI 114 Query: 335 NKVMKDILGRFKELQFFTGESMDCDGML 252 K +L R + QFF GE M D L Sbjct: 115 EGATKFLLPRLSDFQFFVGEGMHDDSTL 142 Score = 52.4 bits (120), Expect = 3e-07 Identities = 25/57 (43%), Positives = 39/57 (68%), Gaps = 1/57 (1%) Frame = -2 Query: 682 MKIYKDIITGDEMFSDTYKMKLVDE-VIYEVTGRLVTRAQGDIQIEGFNPSAEEADE 515 M +Y+D++TGDE+ SD++ K ++ +++EV G+ VT D+ I G NPSAEE E Sbjct: 1 MLVYQDLLTGDELLSDSFPYKEIENGILWEVEGKWVTVGAVDVNI-GANPSAEEGGE 56 >At3g05540.1 68416.m00607 translationally controlled tumor family protein similar to translationally controlled tumor protein GB:AAD10032 from [Hevea brasiliensis] Length = 156 Score = 56.4 bits (130), Expect = 2e-08 Identities = 30/83 (36%), Positives = 48/83 (57%) Frame = -1 Query: 515 GHDSAVESGVDIVLNHRLVETYAFGDKKSYTLYLKDYMKKLVAKLEEKAPDQVEVFKTNM 336 G D VDI+ RL E +F DKK + +++K Y+K+L KL+ + E+FK ++ Sbjct: 46 GVDDQAVKVVDIIDTFRLQEQPSF-DKKQFVMFMKRYIKQLSPKLDS---ENQELFKKHI 101 Query: 335 NKVMKDILGRFKELQFFTGESMD 267 K ++ + K+ QFF GESM+ Sbjct: 102 ESATKFLMSKLKDFQFFVGESME 124 Score = 36.7 bits (81), Expect = 0.016 Identities = 18/52 (34%), Positives = 34/52 (65%), Gaps = 1/52 (1%) Frame = -2 Query: 682 MKIYKDIITGDEMFSDTYKMKLVDE-VIYEVTGRLVTRAQGDIQIEGFNPSA 530 M +Y+DI+TGDE+ SD++ K ++ +++EV G+ + +G + EG + A Sbjct: 1 MLVYQDILTGDELLSDSFPYKEIENGMLWEVEGKNPSGEEGG-EDEGVDDQA 51 >At2g17050.1 68415.m01968 disease resistance protein (TIR-NBS-LRR class), putative domain signature TIR-NBS-LRR exists, suggestive of a disease resistance protein. Length = 1355 Score = 33.5 bits (73), Expect = 0.15 Identities = 25/91 (27%), Positives = 44/91 (48%), Gaps = 8/91 (8%) Frame = -1 Query: 515 GHDSAVESGVDIVLNHRLVETYAFGDKKSYTLYLK-------DYMKKLVAKLEEKAPDQV 357 G D +SG++ + + + Y G+ + +LY + D M+ + KL P + Sbjct: 184 GIDWRKQSGLETLAPYYISVKYFSGNPLALSLYEEMLSHMKSDKMEVKLLKLNHPPPQIM 243 Query: 356 EVFKTNMNKVMKDILGRFKELQ-FFTGESMD 267 EVFK+N N + ++ F ++ FF GE D Sbjct: 244 EVFKSNYNALNENEKSMFLDIACFFRGEKAD 274 >At4g29060.1 68417.m04157 elongation factor Ts family protein similar to SP|P35019 Elongation factor Ts (EF-Ts) {Galdieria sulphuraria}; contains Pfam profiles PF00627: UBA/TS-N domain, PF00889: Elongation factor TS, PF00575: S1 RNA binding domain Length = 953 Score = 31.9 bits (69), Expect = 0.45 Identities = 19/50 (38%), Positives = 30/50 (60%), Gaps = 2/50 (4%) Frame = -2 Query: 655 GDEMFSDTYKMKLVDEVIYEVT--GRLVTRAQGDIQIEGFNPSAEEADEG 512 G+ S K +++D V+ +T G +T +G+ EGF P+AEEAD+G Sbjct: 238 GEGFNSKFAKGQMLDGVVKNLTRSGAFITIGEGE---EGFLPTAEEADDG 284 >At5g51800.1 68418.m06423 expressed protein Length = 972 Score = 29.9 bits (64), Expect = 1.8 Identities = 13/46 (28%), Positives = 26/46 (56%), Gaps = 2/46 (4%) Frame = -2 Query: 703 NRLHPIKMKIYKDIITGDEMFSDTYKMKLVD--EVIYEVTGRLVTR 572 N P++ +YKD+ G E+F +L+D + Y++ G +++R Sbjct: 535 NLRFPLRPDVYKDLPQGKELFFSISSTELLDCRAITYDIIGPIMSR 580 >At5g49030.1 68418.m06067 tRNA synthetase class I (I, L, M and V) family protein similar to SP|P41972 Isoleucyl-tRNA synthetase (EC 6.1.1.5) (Isoleucine--tRNA ligase) (IleRS) {Staphylococcus aureus}; contains Pfam profile PF00133: tRNA synthetases class I (I, L, M and V) Length = 1093 Score = 27.5 bits (58), Expect = 9.6 Identities = 12/31 (38%), Positives = 20/31 (64%), Gaps = 3/31 (9%) Frame = -1 Query: 338 MNKVMKDILGRFKELQFFTGE---SMDCDGM 255 +NK++KDI+ R+K LQ + + DC G+ Sbjct: 167 LNKILKDIINRYKLLQNYKVQYVPGWDCHGL 197 >At1g78390.1 68414.m09135 9-cis-epoxycarotenoid dioxygenase, putative / neoxanthin cleavage enzyme, putative / carotenoid cleavage dioxygenase, putative similar to 9-cis-epoxycarotenoid dioxygenase [Phaseolus vulgaris][GI:6715257]; similar to neoxanthin cleavage enzyme GI:9857290 from [Vigna unguiculata] Length = 657 Score = 27.5 bits (58), Expect = 9.6 Identities = 15/40 (37%), Positives = 22/40 (55%) Frame = +3 Query: 468 VVQDYVNSALDGRVVPSSASSAEGLKPSIWISPCALVTNL 587 VV YV+S+++ + SS+ + KP W C VTNL Sbjct: 95 VVPCYVSSSVNKKSSVSSSLQSPTFKPPSWKKLCNDVTNL 134 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,549,213 Number of Sequences: 28952 Number of extensions: 325482 Number of successful extensions: 891 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 867 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 889 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1584903024 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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