BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ps4M0603.Seq
(725 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g16640.1 68416.m02127 translationally controlled tumor family... 57 1e-08
At3g05540.1 68416.m00607 translationally controlled tumor family... 56 2e-08
At2g17050.1 68415.m01968 disease resistance protein (TIR-NBS-LRR... 33 0.15
At4g29060.1 68417.m04157 elongation factor Ts family protein sim... 32 0.45
At5g51800.1 68418.m06423 expressed protein 30 1.8
At5g49030.1 68418.m06067 tRNA synthetase class I (I, L, M and V)... 27 9.6
At1g78390.1 68414.m09135 9-cis-epoxycarotenoid dioxygenase, puta... 27 9.6
>At3g16640.1 68416.m02127 translationally controlled tumor family
protein similar to translationally controlled tumor
protein GB:AAD10032 from [Hevea brasiliensis]
Length = 168
Score = 56.8 bits (131), Expect = 1e-08
Identities = 36/88 (40%), Positives = 46/88 (52%)
Frame = -1
Query: 515 GHDSAVESGVDIVLNHRLVETYAFGDKKSYTLYLKDYMKKLVAKLEEKAPDQVEVFKTNM 336
G D + + VDIV RL E + DKK + Y+K Y+K L KL E+ DQ VFK +
Sbjct: 59 GVDDSTQKVVDIVDTFRLQEQPTY-DKKGFIAYIKKYIKLLTPKLSEE--DQA-VFKKGI 114
Query: 335 NKVMKDILGRFKELQFFTGESMDCDGML 252
K +L R + QFF GE M D L
Sbjct: 115 EGATKFLLPRLSDFQFFVGEGMHDDSTL 142
Score = 52.4 bits (120), Expect = 3e-07
Identities = 25/57 (43%), Positives = 39/57 (68%), Gaps = 1/57 (1%)
Frame = -2
Query: 682 MKIYKDIITGDEMFSDTYKMKLVDE-VIYEVTGRLVTRAQGDIQIEGFNPSAEEADE 515
M +Y+D++TGDE+ SD++ K ++ +++EV G+ VT D+ I G NPSAEE E
Sbjct: 1 MLVYQDLLTGDELLSDSFPYKEIENGILWEVEGKWVTVGAVDVNI-GANPSAEEGGE 56
>At3g05540.1 68416.m00607 translationally controlled tumor family
protein similar to translationally controlled tumor
protein GB:AAD10032 from [Hevea brasiliensis]
Length = 156
Score = 56.4 bits (130), Expect = 2e-08
Identities = 30/83 (36%), Positives = 48/83 (57%)
Frame = -1
Query: 515 GHDSAVESGVDIVLNHRLVETYAFGDKKSYTLYLKDYMKKLVAKLEEKAPDQVEVFKTNM 336
G D VDI+ RL E +F DKK + +++K Y+K+L KL+ + E+FK ++
Sbjct: 46 GVDDQAVKVVDIIDTFRLQEQPSF-DKKQFVMFMKRYIKQLSPKLDS---ENQELFKKHI 101
Query: 335 NKVMKDILGRFKELQFFTGESMD 267
K ++ + K+ QFF GESM+
Sbjct: 102 ESATKFLMSKLKDFQFFVGESME 124
Score = 36.7 bits (81), Expect = 0.016
Identities = 18/52 (34%), Positives = 34/52 (65%), Gaps = 1/52 (1%)
Frame = -2
Query: 682 MKIYKDIITGDEMFSDTYKMKLVDE-VIYEVTGRLVTRAQGDIQIEGFNPSA 530
M +Y+DI+TGDE+ SD++ K ++ +++EV G+ + +G + EG + A
Sbjct: 1 MLVYQDILTGDELLSDSFPYKEIENGMLWEVEGKNPSGEEGG-EDEGVDDQA 51
>At2g17050.1 68415.m01968 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1355
Score = 33.5 bits (73), Expect = 0.15
Identities = 25/91 (27%), Positives = 44/91 (48%), Gaps = 8/91 (8%)
Frame = -1
Query: 515 GHDSAVESGVDIVLNHRLVETYAFGDKKSYTLYLK-------DYMKKLVAKLEEKAPDQV 357
G D +SG++ + + + Y G+ + +LY + D M+ + KL P +
Sbjct: 184 GIDWRKQSGLETLAPYYISVKYFSGNPLALSLYEEMLSHMKSDKMEVKLLKLNHPPPQIM 243
Query: 356 EVFKTNMNKVMKDILGRFKELQ-FFTGESMD 267
EVFK+N N + ++ F ++ FF GE D
Sbjct: 244 EVFKSNYNALNENEKSMFLDIACFFRGEKAD 274
>At4g29060.1 68417.m04157 elongation factor Ts family protein
similar to SP|P35019 Elongation factor Ts (EF-Ts)
{Galdieria sulphuraria}; contains Pfam profiles PF00627:
UBA/TS-N domain, PF00889: Elongation factor TS, PF00575:
S1 RNA binding domain
Length = 953
Score = 31.9 bits (69), Expect = 0.45
Identities = 19/50 (38%), Positives = 30/50 (60%), Gaps = 2/50 (4%)
Frame = -2
Query: 655 GDEMFSDTYKMKLVDEVIYEVT--GRLVTRAQGDIQIEGFNPSAEEADEG 512
G+ S K +++D V+ +T G +T +G+ EGF P+AEEAD+G
Sbjct: 238 GEGFNSKFAKGQMLDGVVKNLTRSGAFITIGEGE---EGFLPTAEEADDG 284
>At5g51800.1 68418.m06423 expressed protein
Length = 972
Score = 29.9 bits (64), Expect = 1.8
Identities = 13/46 (28%), Positives = 26/46 (56%), Gaps = 2/46 (4%)
Frame = -2
Query: 703 NRLHPIKMKIYKDIITGDEMFSDTYKMKLVD--EVIYEVTGRLVTR 572
N P++ +YKD+ G E+F +L+D + Y++ G +++R
Sbjct: 535 NLRFPLRPDVYKDLPQGKELFFSISSTELLDCRAITYDIIGPIMSR 580
>At5g49030.1 68418.m06067 tRNA synthetase class I (I, L, M and V)
family protein similar to SP|P41972 Isoleucyl-tRNA
synthetase (EC 6.1.1.5) (Isoleucine--tRNA ligase)
(IleRS) {Staphylococcus aureus}; contains Pfam profile
PF00133: tRNA synthetases class I (I, L, M and V)
Length = 1093
Score = 27.5 bits (58), Expect = 9.6
Identities = 12/31 (38%), Positives = 20/31 (64%), Gaps = 3/31 (9%)
Frame = -1
Query: 338 MNKVMKDILGRFKELQFFTGE---SMDCDGM 255
+NK++KDI+ R+K LQ + + DC G+
Sbjct: 167 LNKILKDIINRYKLLQNYKVQYVPGWDCHGL 197
>At1g78390.1 68414.m09135 9-cis-epoxycarotenoid dioxygenase,
putative / neoxanthin cleavage enzyme, putative /
carotenoid cleavage dioxygenase, putative similar to
9-cis-epoxycarotenoid dioxygenase [Phaseolus
vulgaris][GI:6715257]; similar to neoxanthin cleavage
enzyme GI:9857290 from [Vigna unguiculata]
Length = 657
Score = 27.5 bits (58), Expect = 9.6
Identities = 15/40 (37%), Positives = 22/40 (55%)
Frame = +3
Query: 468 VVQDYVNSALDGRVVPSSASSAEGLKPSIWISPCALVTNL 587
VV YV+S+++ + SS+ + KP W C VTNL
Sbjct: 95 VVPCYVSSSVNKKSSVSSSLQSPTFKPPSWKKLCNDVTNL 134
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,549,213
Number of Sequences: 28952
Number of extensions: 325482
Number of successful extensions: 891
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 867
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 889
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1584903024
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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