BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ps4M0528.Seq (964 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomy... 30 0.42 SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 27 3.0 SPCC16A11.13 |usp106|luc7|U1 snRNP-associated protein Usp106|Sch... 27 3.0 SPBC17D11.01 |nep1||nedd8 protease Nep1|Schizosaccharomyces pomb... 26 6.9 SPBC32H8.01c ||SPBP22H7.10c|conserved fungal protein|Schizosacch... 26 9.1 >SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomyces pombe|chr 2|||Manual Length = 542 Score = 30.3 bits (65), Expect = 0.42 Identities = 20/74 (27%), Positives = 39/74 (52%), Gaps = 5/74 (6%) Frame = +3 Query: 306 STGDFFDGVENYIQSHDVTFKLP---IADTQITVS--PRNLDNDELTLNVKFNKEGARSV 470 S GDF+D + + S+ T+ +P + + + ++ +L++ + TLN + N+E S+ Sbjct: 73 SMGDFYDDIRLMV-SNAQTYNMPGSLVYECSVLIANTANSLESKDGTLNEEENEEMESSI 131 Query: 471 GEARKAKLKKIIVP 512 E K +I VP Sbjct: 132 NEEHKPGTNEIDVP 145 >SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1647 Score = 27.5 bits (58), Expect = 3.0 Identities = 14/44 (31%), Positives = 23/44 (52%) Frame = -1 Query: 643 EDSQSHGDNEGEERQLKSVPSLQTEDTDSQWDEGHGLEQNEHQD 512 +D +S +E ER + V + ED D DEG ++NE+ + Sbjct: 233 KDDESSSGSESYERD-EDVDEEEEEDDDENNDEGDDEDENENDE 275 >SPCC16A11.13 |usp106|luc7|U1 snRNP-associated protein Usp106|Schizosaccharomyces pombe|chr 3|||Manual Length = 264 Score = 27.5 bits (58), Expect = 3.0 Identities = 13/41 (31%), Positives = 24/41 (58%) Frame = -1 Query: 679 VRVVGRDLLAKLEDSQSHGDNEGEERQLKSVPSLQTEDTDS 557 +R + RDL A+LED + D + E+Q ++ S + + + S Sbjct: 221 LRNIARDLRAQLEDREKSRDKKDGEKQRDNLASFEDKISTS 261 >SPBC17D11.01 |nep1||nedd8 protease Nep1|Schizosaccharomyces pombe|chr 2|||Manual Length = 420 Score = 26.2 bits (55), Expect = 6.9 Identities = 13/46 (28%), Positives = 22/46 (47%) Frame = +3 Query: 288 NDVTGRSTGDFFDGVENYIQSHDVTFKLPIADTQITVSPRNLDNDE 425 N + R +GDF + D+ LP+ D + V P++ D+ E Sbjct: 218 NSLGTRVSGDFDEDFPTGTVFFDLESHLPLLDVALPVLPKSSDSSE 263 >SPBC32H8.01c ||SPBP22H7.10c|conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 187 Score = 25.8 bits (54), Expect = 9.1 Identities = 15/62 (24%), Positives = 27/62 (43%) Frame = -1 Query: 706 PRTVGSDLRVRVVGRDLLAKLEDSQSHGDNEGEERQLKSVPSLQTEDTDSQWDEGHGLEQ 527 P +V + ++ L AK E D G ++ K P+L+ + T+ + D + Sbjct: 63 PISVIKSVTNKIDTNKLEAKDESKNVEKDATGNPQESKQEPNLEVQQTEKEKDVDSASAE 122 Query: 526 NE 521 NE Sbjct: 123 NE 124 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,742,762 Number of Sequences: 5004 Number of extensions: 73674 Number of successful extensions: 214 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 212 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 214 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 493304942 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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