Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ps4M0528.Seq
(964 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g09720.2 68417.m01597 Ras-related GTP-binding protein, putati... 31 0.86
At4g09720.1 68417.m01596 Ras-related GTP-binding protein, putati... 31 0.86
At4g02150.1 68417.m00287 importin alpha-2 subunit identical to i... 31 1.1
At5g19900.1 68418.m02368 PRLI-interacting factor, putative stron... 30 2.0
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 30 2.0
At5g53040.1 68418.m06588 RWP-RK domain-containing protein contai... 29 3.5
At3g06720.2 68416.m00797 importin alpha-1 subunit, putative (IMP... 29 3.5
At3g06720.1 68416.m00796 importin alpha-1 subunit, putative (IMP... 29 3.5
At1g32410.2 68414.m04000 vacuolar protein sorting 55 family prot... 29 3.5
At1g32410.1 68414.m03999 vacuolar protein sorting 55 family prot... 29 3.5
At3g09000.1 68416.m01053 proline-rich family protein 29 4.6
At1g32160.1 68414.m03956 expressed protein 28 8.0
>At4g09720.2 68417.m01597 Ras-related GTP-binding protein, putative
similar to GTP-binding protein RAB7A from [Lotus
japonicus]
Length = 172
Score = 31.5 bits (68), Expect = 0.86
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = -3
Query: 275 LRHFDNIEIWKVSFLKMESSSDMTLYLMQAVGS 177
LR FDN+E W FLK S SD + +G+
Sbjct: 59 LRSFDNLETWHEEFLKQASPSDPKTFPFIVLGN 91
>At4g09720.1 68417.m01596 Ras-related GTP-binding protein, putative
similar to GTP-binding protein RAB7A from [Lotus
japonicus]
Length = 206
Score = 31.5 bits (68), Expect = 0.86
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = -3
Query: 275 LRHFDNIEIWKVSFLKMESSSDMTLYLMQAVGS 177
LR FDN+E W FLK S SD + +G+
Sbjct: 93 LRSFDNLETWHEEFLKQASPSDPKTFPFIVLGN 125
>At4g02150.1 68417.m00287 importin alpha-2 subunit identical to
importin alpha-2 subunit (Karyopherin alpha-2 subunit)
(KAP alpha) SP:O04294 from [Arabidopsis thaliana]
Length = 531
Score = 31.1 bits (67), Expect = 1.1
Identities = 14/50 (28%), Positives = 30/50 (60%)
Frame = -1
Query: 655 LAKLEDSQSHGDNEGEERQLKSVPSLQTEDTDSQWDEGHGLEQNEHQDGT 506
L K+E+ QSH +N+ ++ +K + + TED + + ++ + Q+ Q G+
Sbjct: 471 LEKIENLQSHDNNDIYDKAVKILETFWTEDNEEEGNDENHAPQSGFQFGS 520
>At5g19900.1 68418.m02368 PRLI-interacting factor, putative strong
similarity to PRLI-interacting factor A [Arabidopsis
thaliana] GI:11139262
Length = 494
Score = 30.3 bits (65), Expect = 2.0
Identities = 22/78 (28%), Positives = 34/78 (43%), Gaps = 2/78 (2%)
Frame = +3
Query: 255 DVVEVTKNGAGNDVTGRSTGDFFDGVENYIQSHDVTFKLPIADTQITVSPRNLDNDELTL 434
+VVE G+D G + EN++ + DV + A V N +N +
Sbjct: 363 EVVENESESDGDDTGGSDARTSGETKENHVAAEDVCMQDVAARDNEAVVKENNNNSKSVD 422
Query: 435 NVKFNKE--GARSVGEAR 482
VK NK+ G S GE++
Sbjct: 423 EVKANKQSVGVGSSGESK 440
>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
protein contains Pfam profile PF01480: PWI domain
Length = 878
Score = 30.3 bits (65), Expect = 2.0
Identities = 20/66 (30%), Positives = 23/66 (34%)
Frame = +1
Query: 676 APSNRCPRSLGRRLRSNPPTXXRLNPKESPRNGLQRLPXRXXKHXPKPSIFPXRKPSANE 855
A R P RR RS PP R +P R P + P P R PS
Sbjct: 347 ARQRRSPSPPARRHRSPPPARRRRSPSPPARRRRSPSPPARRRRSPSPLYRRNRSPSPLY 406
Query: 856 RCKKIR 873
R + R
Sbjct: 407 RRNRSR 412
Score = 28.7 bits (61), Expect = 6.1
Identities = 22/71 (30%), Positives = 26/71 (36%), Gaps = 3/71 (4%)
Frame = +1
Query: 655 EDRGRQLAPSNR--CPRSLGRRLRS-NPPTXXRLNPKESPRNGLQRLPXRXXKHXPKPSI 825
E R + APS R P RR RS +PP R +P R P + P P
Sbjct: 298 EGRRQSPAPSRRRRSPSPPARRRRSPSPPARRRRSPSPPARRHRSPTPPARQRRSPSPPA 357
Query: 826 FPXRKPSANER 858
R P R
Sbjct: 358 RRHRSPPPARR 368
>At5g53040.1 68418.m06588 RWP-RK domain-containing protein contains
Pfam profile: PF02042 RWP-RK domain
Length = 256
Score = 29.5 bits (63), Expect = 3.5
Identities = 23/79 (29%), Positives = 35/79 (44%), Gaps = 4/79 (5%)
Frame = +3
Query: 315 DFFDGVE--NYIQS--HDVTFKLPIADTQITVSPRNLDNDELTLNVKFNKEGARSVGEAR 482
DFF+ +E N I S H +T + + + SP + ND L NVK K + +
Sbjct: 82 DFFENIEVDNTIPSDIHLLTQEPYFSSDSSSSSPLAIQNDGLISNVKVEKVTVKKKRNLK 141
Query: 483 KAKLKKIIVPSWCSFCSRP 539
K + K+ + F RP
Sbjct: 142 KKRQDKLEMSEIKQFFDRP 160
>At3g06720.2 68416.m00797 importin alpha-1 subunit, putative (IMPA1)
similar to importin alpha-1 subunit (Karyopherin alpha-1
subunit, KAP alpha) [Arabidopsis thaliana]
SWISS-PROT:Q96321
Length = 532
Score = 29.5 bits (63), Expect = 3.5
Identities = 13/45 (28%), Positives = 27/45 (60%)
Frame = -1
Query: 655 LAKLEDSQSHGDNEGEERQLKSVPSLQTEDTDSQWDEGHGLEQNE 521
L K+E+ QSH +NE E+ +K + + E+ D + + G++ ++
Sbjct: 468 LEKIENLQSHDNNEIYEKAVKILETYWLEEEDDETQQPPGVDGSQ 512
>At3g06720.1 68416.m00796 importin alpha-1 subunit, putative (IMPA1)
similar to importin alpha-1 subunit (Karyopherin alpha-1
subunit, KAP alpha) [Arabidopsis thaliana]
SWISS-PROT:Q96321
Length = 532
Score = 29.5 bits (63), Expect = 3.5
Identities = 13/45 (28%), Positives = 27/45 (60%)
Frame = -1
Query: 655 LAKLEDSQSHGDNEGEERQLKSVPSLQTEDTDSQWDEGHGLEQNE 521
L K+E+ QSH +NE E+ +K + + E+ D + + G++ ++
Sbjct: 468 LEKIENLQSHDNNEIYEKAVKILETYWLEEEDDETQQPPGVDGSQ 512
>At1g32410.2 68414.m04000 vacuolar protein sorting 55 family protein
/ VPS55 family protein contains Pfam domain PF04133:
Vacuolar protein sorting 55
Length = 140
Score = 29.5 bits (63), Expect = 3.5
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = +2
Query: 548 IPLAIGVLGLKAWNALQLSFFSFVVSVALAIFQLCKKIAADNSHPQI 688
IP + GL W AL L S+VV + + +C A+DN + I
Sbjct: 94 IPSILKHAGLIGWGALALDLSSYVVFLVAILGYICIGDASDNYYSYI 140
>At1g32410.1 68414.m03999 vacuolar protein sorting 55 family protein
/ VPS55 family protein contains Pfam domain PF04133:
Vacuolar protein sorting 55
Length = 140
Score = 29.5 bits (63), Expect = 3.5
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = +2
Query: 548 IPLAIGVLGLKAWNALQLSFFSFVVSVALAIFQLCKKIAADNSHPQI 688
IP + GL W AL L S+VV + + +C A+DN + I
Sbjct: 94 IPSILKHAGLIGWGALALDLSSYVVFLVAILGYICIGDASDNYYSYI 140
>At3g09000.1 68416.m01053 proline-rich family protein
Length = 541
Score = 29.1 bits (62), Expect = 4.6
Identities = 17/42 (40%), Positives = 19/42 (45%)
Frame = -1
Query: 334 STPSKKSPVDRPVTSLPAPFFVTSTTSRSGKCPS*KWSPVAT 209
S+ S K PV RP T P T + S K PS SP T
Sbjct: 230 SSASSKKPVSRPATPTRRPSTPTGPSIVSSKAPSRGTSPSPT 271
>At1g32160.1 68414.m03956 expressed protein
Length = 406
Score = 28.3 bits (60), Expect = 8.0
Identities = 23/81 (28%), Positives = 36/81 (44%)
Frame = +3
Query: 246 PDLDVVEVTKNGAGNDVTGRSTGDFFDGVENYIQSHDVTFKLPIADTQITVSPRNLDNDE 425
P L +NG G VT R++GD D EN+ V + P+ + +
Sbjct: 45 PKLGSSSTNENGRGRSVTVRASGD-EDSNENFAPLAPVELESPVGQLLEQILRTHPHLLP 103
Query: 426 LTLNVKFNKEGARSVGEARKA 488
+T++ + K A S E+RKA
Sbjct: 104 VTVDEQLEKFAAES--ESRKA 122
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,157,458
Number of Sequences: 28952
Number of extensions: 419243
Number of successful extensions: 1357
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1294
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1354
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2324382072
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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