Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ps4M0516.Seq
(976 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g34200.1 68417.m04854 D-3-phosphoglycerate dehydrogenase, put... 61 1e-09
At1g17745.1 68414.m02196 D-3-phosphoglycerate dehydrogenase / 3-... 60 2e-09
At5g14780.1 68418.m01734 formate dehydrogenase (FDH) identical t... 59 5e-09
At1g72190.1 68414.m08347 oxidoreductase family protein similar t... 54 2e-07
At1g79870.1 68414.m09330 oxidoreductase family protein contains ... 50 2e-06
At1g12550.1 68414.m01455 oxidoreductase family protein similar t... 48 7e-06
At2g45630.2 68415.m05674 oxidoreductase family protein low simil... 47 2e-05
At3g19480.1 68416.m02469 D-3-phosphoglycerate dehydrogenase, put... 42 6e-04
At1g01510.1 68414.m00067 C-terminal binding protein (ANGUSTIFOLI... 35 0.071
At4g11000.1 68417.m01789 ankyrin repeat family protein contains ... 31 1.5
At5g20610.1 68418.m02448 expressed protein 30 2.0
At5g58910.1 68418.m07380 laccase, putative / diphenol oxidase, p... 30 2.7
At5g61470.1 68418.m07713 zinc finger (C2H2 type) family protein ... 28 8.2
At4g03250.1 68417.m00444 homeobox-leucine zipper family protein ... 28 8.2
>At4g34200.1 68417.m04854 D-3-phosphoglycerate dehydrogenase,
putative / 3-PGDH, putative similar to phosphoglycerate
dehydrogenase, Arabidopsis thaliana, SP:O04130
Length = 603
Score = 60.9 bits (141), Expect = 1e-09
Identities = 38/102 (37%), Positives = 55/102 (53%)
Frame = +3
Query: 171 LPNTPETVGIINQQLLEKLPDGAYLLNLRVVFMLWKMTCSRRWIAGKVKGAMLDVFNREP 350
+P TP T I+N + K+ G ++N+ ++ + R AG V A LDVF +EP
Sbjct: 262 MPLTPTTSKILNDETFAKMKKGVRIVNVARGGVIDEDALVRALDAGIVAQAALDVFTKEP 321
Query: 351 LPPESPLWQHPRVTITPHVAAITRPAEAVEYISRTIAQLEKG 476
+S L QH RVT+TPH+ A T EA E ++ IA+ G
Sbjct: 322 PAKDSKLVQHERVTVTPHLGAST--MEAQEGVAIEIAEAVVG 361
>At1g17745.1 68414.m02196 D-3-phosphoglycerate dehydrogenase /
3-PGDH identical to SP|O04130
Length = 624
Score = 60.5 bits (140), Expect = 2e-09
Identities = 39/106 (36%), Positives = 56/106 (52%), Gaps = 3/106 (2%)
Frame = +3
Query: 171 LPNTPETVGIINQQLLEKLPDGAYLLNLRVVFMLWKMTCSRRWIAGKVKGAMLDVFNREP 350
+P TP T + N + K+ G L+N+ ++ + R AG V A LDVF EP
Sbjct: 283 MPLTPATKKVFNDETFSKMKKGVRLINVARGGVIDEDALVRALDAGIVAQAALDVFCEEP 342
Query: 351 LPPESPLWQHPRVTITPHVAAITRPAE---AVEYISRTIAQLEKGE 479
+S L QH VT+TPH+ A T+ A+ A+E I+ +A KGE
Sbjct: 343 PSKDSRLIQHENVTVTPHLGASTKEAQEGVAIE-IAEAVAGALKGE 387
>At5g14780.1 68418.m01734 formate dehydrogenase (FDH) identical to
GI:7677266
Length = 384
Score = 58.8 bits (136), Expect = 5e-09
Identities = 42/160 (26%), Positives = 79/160 (49%), Gaps = 3/160 (1%)
Frame = +3
Query: 3 LGAGVLGSKVAQSLQTWRFPLRCWSRTRKSWPGVQSFAGR---EELSAFLSQCRVLINLL 173
+GAG +G + Q L+ + L R + + P ++ G E+L+ L +C V++ +
Sbjct: 203 VGAGRIGKLLLQRLKPFGCNLLYHDRLQMA-PELEKETGAKFVEDLNEMLPKCDVIVINM 261
Query: 174 PNTPETVGIINQQLLEKLPDGAYLLNLRVVFMLWKMTCSRRWIAGKVKGAMLDVFNREPL 353
P T +T G+ N++L+ KL G ++N ++ + +G + G DV++ +P
Sbjct: 262 PLTEKTRGMFNKELIGKLKKGVLIVNNARGAIMERQAVVDAVESGHIGGYSGDVWDPQPA 321
Query: 354 PPESPLWQHPRVTITPHVAAITRPAEAVEYISRTIAQLEK 473
P + P P +TPH + T A+ + Y + T LE+
Sbjct: 322 PKDHPWRYMPNQAMTPHTSGTTIDAQ-LRYAAGTKDMLER 360
>At1g72190.1 68414.m08347 oxidoreductase family protein similar to
D-3-phosphoglycerate dehydrogenase from Arabidopsis
thaliana [SP|O04130], glyoxylate reductase from Homo
sapiens (gi:6002730); contains Pfam D-isomer specific
2-hydroxyacid dehydrogenase, NAD binding domain PF02826
Length = 373
Score = 53.6 bits (123), Expect = 2e-07
Identities = 43/164 (26%), Positives = 78/164 (47%), Gaps = 9/164 (5%)
Frame = +3
Query: 3 LGAGVLGSKVAQSLQTWRFPLRCWSRTRKSWPG---------VQSFAGREELSAFLSQCR 155
LG G +G ++A+ L+ F R + T++ WP V E++ F +
Sbjct: 198 LGYGNIGIELAKRLKP--FGSRVIA-TKRFWPASIVDSDSRLVDEKGSHEDIYTFAGKAD 254
Query: 156 VLINLLPNTPETVGIINQQLLEKLPDGAYLLNLRVVFMLWKMTCSRRWIAGKVKGAMLDV 335
+++ L ET I+N++ + + GA L+N+ ++ + + +G + G +DV
Sbjct: 255 IVVVCLRLNKETAEIVNKEFICSMKKGALLVNIARGGLINYESAFQNLESGHLGGLGIDV 314
Query: 336 FNREPLPPESPLWQHPRVTITPHVAAITRPAEAVEYISRTIAQL 467
EP P P+ + V ITPHVA +T EY R++A++
Sbjct: 315 AWSEPFDPNDPILKFKNVIITPHVAGVT------EYSYRSMAKI 352
>At1g79870.1 68414.m09330 oxidoreductase family protein contains
Pfam profile: PF02826 D-isomer specific 2-hydroxyacid
dehydrogenase, NAD binding domain; similar to glyoxylate
reductase from Thermococcus litoralis [gi:13515409]
Length = 313
Score = 50.4 bits (115), Expect = 2e-06
Identities = 36/139 (25%), Positives = 69/139 (49%)
Frame = +3
Query: 3 LGAGVLGSKVAQSLQTWRFPLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNT 182
+G G +G+ +A+ + + P+ +SRT K + + +L+ +L+ P T
Sbjct: 150 IGLGRIGTAIAKRAEAFSCPINYYSRTIKPDVAYKYYPTVVDLA---QNSDILVVACPLT 206
Query: 183 PETVGIINQQLLEKLPDGAYLLNLRVVFMLWKMTCSRRWIAGKVKGAMLDVFNREPLPPE 362
+T I+++Q+++ L L+N+ + + + G++ GA LDVF +EP PE
Sbjct: 207 EQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQELIKALTEGRLGGAALDVFEQEPHVPE 266
Query: 363 SPLWQHPRVTITPHVAAIT 419
L+ V + PHV + T
Sbjct: 267 E-LFGLENVVLLPHVGSGT 284
>At1g12550.1 68414.m01455 oxidoreductase family protein similar to
glyoxylate reductase from Homo sapiens (gi:6002730);
contains Pfam D-isomer specific 2-hydroxyacid
dehydrogenase, NAD binding domain PF02826
Length = 323
Score = 48.4 bits (110), Expect = 7e-06
Identities = 36/139 (25%), Positives = 70/139 (50%)
Frame = +3
Query: 3 LGAGVLGSKVAQSLQTWRFPLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNT 182
+G G +GS VA+ L+++ + SR++K + ++ ++ + VL+ T
Sbjct: 158 VGLGSIGSFVAKRLESFGCVISYNSRSQKQSSPYRYYS---DILSLAENNDVLVLCCSLT 214
Query: 183 PETVGIINQQLLEKLPDGAYLLNLRVVFMLWKMTCSRRWIAGKVKGAMLDVFNREPLPPE 362
ET I+N++++E L ++N+ ++ + + + G + GA LDVF EP P+
Sbjct: 215 DETHHIVNREVMELLGKDGVVINVGRGKLIDEKEMVKCLVDGVIGGAGLDVFENEPAVPQ 274
Query: 363 SPLWQHPRVTITPHVAAIT 419
L+ V ++PH A T
Sbjct: 275 E-LFGLDNVVLSPHFAVAT 292
>At2g45630.2 68415.m05674 oxidoreductase family protein low
similarity to SP|P36234 Glycerate dehydrogenase (EC
1.1.1.29) (NADH-dependent hydroxypyruvate reductase)
{Hyphomicrobium methylovorum}; contains Pfam profile
PF00389: D-isomer specific 2-hydroxyacid dehydrogenase,
catalytic domain
Length = 338
Score = 46.8 bits (106), Expect = 2e-05
Identities = 42/153 (27%), Positives = 72/153 (47%), Gaps = 1/153 (0%)
Frame = +3
Query: 3 LGAGVLGSKVAQSLQTWRFPLRCWSRTRKSWP-GVQSFAGREELSAFLSQCRVLINLLPN 179
+G G +GSKVA L + + SR RK + + EE++A LI
Sbjct: 174 VGLGSIGSKVATRLDAFGCQISYSSRNRKPYDVPYHYYMDIEEMAA---NSDALIICCEL 230
Query: 180 TPETVGIINQQLLEKLPDGAYLLNLRVVFMLWKMTCSRRWIAGKVKGAMLDVFNREPLPP 359
+T+ +IN+ +L L ++N+ ++ + R G++ GA LDVF EP P
Sbjct: 231 NEKTLRLINKDVLSALGKRGVIVNVARGAIIDEEEMVRCLREGEIGGAGLDVFEDEPNVP 290
Query: 360 ESPLWQHPRVTITPHVAAITRPAEAVEYISRTI 458
+ L++ V +PH A +T E +E + + +
Sbjct: 291 KE-LFELDNVVFSPHSAFMT--LEGLEELGKVV 320
>At3g19480.1 68416.m02469 D-3-phosphoglycerate dehydrogenase,
putative / 3-PGDH, putative similar to SP:O04130 from
[Arabidopsis thaliana]
Length = 588
Score = 41.9 bits (94), Expect = 6e-04
Identities = 31/98 (31%), Positives = 47/98 (47%)
Frame = +3
Query: 171 LPNTPETVGIINQQLLEKLPDGAYLLNLRVVFMLWKMTCSRRWIAGKVKGAMLDVFNREP 350
LP T T ++N + G ++N+ ++ + R +G V A LDVF EP
Sbjct: 247 LPLTAATSKMMNDVTFAMMKKGVRIVNVARGGVIDEEALLRALDSGIVAQAALDVFTVEP 306
Query: 351 LPPESPLWQHPRVTITPHVAAITRPAEAVEYISRTIAQ 464
++ L H VT TPH+ A T EA E +S +A+
Sbjct: 307 PVKDNKLVLHESVTATPHLGAST--MEAQEGVSIEVAE 342
>At1g01510.1 68414.m00067 C-terminal binding protein (ANGUSTIFOLIA)
nearly identical to C-terminal binding protein
ANGUSTIFOLIA [Arabidopsis thaliana] GI:15408535;
contains Pfam profile PF02826: D-isomer specific
2-hydroxyacid dehydrogenase, NAD binding domain
Length = 636
Score = 35.1 bits (77), Expect = 0.071
Identities = 25/89 (28%), Positives = 39/89 (43%)
Frame = +3
Query: 180 TPETVGIINQQLLEKLPDGAYLLNLRVVFMLWKMTCSRRWIAGKVKGAMLDVFNREPLPP 359
T +TV I+N + L+ + GA+L+N +L + I G + G LD
Sbjct: 234 TNDTVQILNAECLQHIKPGAFLVNTGSCQLLDDCAVKQLLIDGTIAGCALD-------GA 286
Query: 360 ESPLWQHPRVTITPHVAAITRPAEAVEYI 446
E P W V P+V + R A+ E +
Sbjct: 287 EGPQWMEAWVKEMPNVLILPRSADYSEEV 315
>At4g11000.1 68417.m01789 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 406
Score = 30.7 bits (66), Expect = 1.5
Identities = 16/49 (32%), Positives = 27/49 (55%)
Frame = +3
Query: 402 HVAAITRPAEAVEYISRTIAQLEKGERVAGKSTAQRLLIKHQDSCYPWA 548
H+AA+ E ++ + +T+ K + + GK TA +L HQ C+P A
Sbjct: 150 HIAALINQTEVMKLLRKTVKV--KAKNLDGK-TAMDILQTHQSPCFPVA 195
>At5g20610.1 68418.m02448 expressed protein
Length = 1164
Score = 30.3 bits (65), Expect = 2.0
Identities = 23/71 (32%), Positives = 34/71 (47%), Gaps = 6/71 (8%)
Frame = +3
Query: 303 AGKVKGAMLDVFNREPLPPESPLWQHPRVTI------TPHVAAITRPAEAVEYISRTIAQ 464
AG+V A D E LPPE PL + A+I R EAVE ++ +A
Sbjct: 408 AGEVPTAGRDEVGTEILPPEEPLVNGNETDVPFEELMITGEASIARSEEAVEIVTEELAP 467
Query: 465 LEKGERVAGKS 497
E+G +++ K+
Sbjct: 468 -EEGNKISPKN 477
>At5g58910.1 68418.m07380 laccase, putative / diphenol oxidase,
putative similar to diphenol oxidase [Nicotiana
tabacum][GI:1685087]
Length = 523
Score = 29.9 bits (64), Expect = 2.7
Identities = 11/25 (44%), Positives = 17/25 (68%), Gaps = 1/25 (4%)
Frame = -3
Query: 449 RNVLHSFSGTGNRGDMWCYRH-AWM 378
+N LH+F+ TG RG +W + H W+
Sbjct: 68 QNYLHNFTLTGQRGTLWWHAHILWL 92
>At5g61470.1 68418.m07713 zinc finger (C2H2 type) family protein
contains Pfam profile: PF00096 zinc finger, C2H2 type
Length = 304
Score = 28.3 bits (60), Expect = 8.2
Identities = 16/51 (31%), Positives = 26/51 (50%), Gaps = 6/51 (11%)
Frame = -3
Query: 149 LAQKCRQFFPSGKAL------HARPRFTGSTPATQRKAPGLQTLSNFTAQY 15
+ Q C++ F +GKAL H F GS+ +T P L++ +N A +
Sbjct: 10 ICQVCKKSFSNGKALGGHMRAHTLKFFPGSSSSTGSITPSLRSCNNLGASF 60
>At4g03250.1 68417.m00444 homeobox-leucine zipper family protein
similar to homeobox transcription factor Hox7
[Lycopersicon peruvianum] GI:19486; contains Pfam
PF00046: Homeobox domain
Length = 476
Score = 28.3 bits (60), Expect = 8.2
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = -2
Query: 588 RSTGYITLSCIQFPPKDSRNPDAL 517
R TGY+T S PP SR+P+++
Sbjct: 364 RKTGYLTKSSKLLPPSRSRSPESM 387
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,574,564
Number of Sequences: 28952
Number of extensions: 441626
Number of successful extensions: 1082
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 1039
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1078
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2363283864
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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