Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ps4M0448.Seq
         (889 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   157   1e-39
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   156   3e-39
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    72   1e-13
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...    72   1e-13
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   8.2  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   8.2  

>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  157 bits (382), Expect = 1e-39
 Identities = 83/190 (43%), Positives = 105/190 (55%)
 Frame = +2

Query: 257 EVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFL 436
           +VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RIR++AD C+GLQGFL
Sbjct: 81  QVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFL 140

Query: 437 IFHXXXXXXXXXXXXLLMERLSVDTARSLNWXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 616
           +FH            LL+ERL+++  +  N                              
Sbjct: 141 VFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLD 200

Query: 617 XXXXXXWSTMKPSMTSAAVISTLSAQPYTNLNRLIGQIVSSITASLRXXGALNVDFXEFQ 796
                     +           +    Y NLNRLI Q+VSSITASLR  G+LNVD  EFQ
Sbjct: 201 NSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQ 260

Query: 797 TNXGGLTPYP 826
           TN   L PYP
Sbjct: 261 TN---LVPYP 267



 Score =  114 bits (274), Expect = 2e-26
 Identities = 49/63 (77%), Positives = 57/63 (90%)
 Frame = +1

Query: 508 HGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICXRNLDIE 687
           +GKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FMVDNEA YDIC RNLDIE
Sbjct: 165 YGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIE 224

Query: 688 RXT 696
           R T
Sbjct: 225 RPT 227



 Score =  113 bits (271), Expect = 4e-26
 Identities = 55/80 (68%), Positives = 61/80 (76%), Gaps = 5/80 (6%)
 Frame = +3

Query: 30  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 194
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSE
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 195 TGAGKHVPRAVFVDLEPTVV 254
           TG GK VPR+++VDLEP V+
Sbjct: 60  TGQGKFVPRSIYVDLEPNVI 79


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  156 bits (379), Expect = 3e-39
 Identities = 85/190 (44%), Positives = 103/190 (54%)
 Frame = +2

Query: 257 EVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFL 436
           +VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR++AD C+GLQGFL
Sbjct: 77  QVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFL 136

Query: 437 IFHXXXXXXXXXXXXLLMERLSVDTARSLNWXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 616
           +FH            LL+ERL+++  +                                 
Sbjct: 137 VFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLD 196

Query: 617 XXXXXXWSTMKPSMTSAAVISTLSAQPYTNLNRLIGQIVSSITASLRXXGALNVDFXEFQ 796
                     +           +    Y NLNRLI Q+VSSITASLR  G+LNVD  EFQ
Sbjct: 197 LADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQ 256

Query: 797 TNXGGLTPYP 826
           TN   L PYP
Sbjct: 257 TN---LVPYP 263



 Score =  120 bits (289), Expect = 3e-28
 Identities = 51/75 (68%), Positives = 60/75 (80%)
 Frame = +3

Query: 30  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 209
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 210 HVPRAVFVDLEPTVV 254
           +VPR+++VDLEP V+
Sbjct: 61  YVPRSIYVDLEPNVI 75



 Score =  107 bits (257), Expect = 2e-24
 Identities = 46/59 (77%), Positives = 54/59 (91%)
 Frame = +1

Query: 514 KKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICXRNLDIER 690
           KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVDNE+ YDIC RNLDIER
Sbjct: 163 KKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIER 221


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 72.1 bits (169), Expect = 1e-13
 Identities = 36/95 (37%), Positives = 54/95 (56%)
 Frame = +3

Query: 33  RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 212
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++   ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60

Query: 213 VPRAVFVDLEPTVVMRSALXHTDSCFIQNNLLLVR 317
           +PRA+ +DLEP VV         S +   N+L+ +
Sbjct: 61  IPRAILIDLEPRVVNNILSDTYGSLYNPENILITK 95



 Score = 44.0 bits (99), Expect = 3e-05
 Identities = 25/83 (30%), Positives = 42/83 (50%), Gaps = 2/83 (2%)
 Frame = +2

Query: 260 VRTGTYRQLFHPEQLITGKED--AANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGF 433
           + + TY  L++PE ++  K    A NN+A G Y+  + I + ++D I + AD    L+GF
Sbjct: 77  ILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREADGSDSLEGF 135

Query: 434 LIFHXXXXXXXXXXXXLLMERLS 502
            + H             L+ERL+
Sbjct: 136 SLLHSIAGGTGSGLGSFLLERLN 158



 Score = 42.3 bits (95), Expect = 9e-05
 Identities = 25/77 (32%), Positives = 41/77 (53%), Gaps = 1/77 (1%)
 Frame = +1

Query: 478 FLIDGASLR*HGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAI 654
           FL++  + R + KK    ++++P  Q VS  VV+PYNS+L       ++D   ++DN A+
Sbjct: 152 FLLERLNDR-YPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAAL 210

Query: 655 YDICXRNLDIERXTLHQ 705
             I    L  +  T HQ
Sbjct: 211 AHIAADRLHTQNPTFHQ 227


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score = 71.7 bits (168), Expect = 1e-13
 Identities = 45/189 (23%), Positives = 76/189 (40%)
 Frame = +2

Query: 260 VRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLI 439
           V++G +  LF P+ +I G+  A N +A+GHYT G E+ D VLD +R+ A+ C  LQGF +
Sbjct: 76  VKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQL 135

Query: 440 FHXXXXXXXXXXXXLLMERLSVDTARSLNWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 619
            H            LL+ ++  +    +                                
Sbjct: 136 THSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVEN 195

Query: 620 XXXXXWSTMKPSMTSAAVISTLSAQPYTNLNRLIGQIVSSITASLRXXGALNVDFXEFQT 799
                    +   +  A    + +  Y +LN L+  +++ +T S R  G LN D  +   
Sbjct: 196 SDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAV 255

Query: 800 NXGGLTPYP 826
           N   + P+P
Sbjct: 256 N---MVPFP 261



 Score = 60.1 bits (139), Expect = 4e-10
 Identities = 30/72 (41%), Positives = 38/72 (52%)
 Frame = +3

Query: 30  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 209
           MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E   GK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 210 HVPRAVFVDLEP 245
           +VPRAV VDLEP
Sbjct: 59  YVPRAVLVDLEP 70



 Score = 53.2 bits (122), Expect = 5e-08
 Identities = 24/52 (46%), Positives = 34/52 (65%)
 Frame = +1

Query: 532 FAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICXRNLDIE 687
           F++ PAP+ S  VVEPYN+ L+ H  +E+SD  F +DNEA+  I    L I+
Sbjct: 167 FSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIK 218


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 25.8 bits (54), Expect = 8.2
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = +1

Query: 109 STASSLMARCPQTRPSGVETILSTLSSARPELAST 213
           ST SSL +    ++PS   T  ST SSA P   S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 8.2
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +2

Query: 128 WPDAHRQDHRGWRRFFQHFLQR 193
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,489,187
Number of Sequences: 5004
Number of extensions: 70152
Number of successful extensions: 199
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 180
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 196
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 446488370
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)