BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ps4M0444.Seq (612 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAPB1E7.12 |rps602|rps6-2, rps6|40S ribosomal protein S6|Schizo... 132 4e-32 SPAC13G6.07c |rps601|rps6-1|40S ribosomal protein S6|Schizosacch... 132 5e-32 SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 29 0.70 SPBC31F10.13c |hip1|hir1|hira protein Hip1|Schizosaccharomyces p... 27 2.1 SPCC1450.06c |grx3||monothiol glutaredoxin Grx3|Schizosaccharomy... 26 3.7 SPAC6F6.02c |pof5||F-box protein Pof5|Schizosaccharomyces pombe|... 25 6.5 SPCC576.10c |rpt3||19S proteasome regulatory subunit Rpt3|Schizo... 25 8.6 SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomy... 25 8.6 SPAC1B3.13 |||U3 snoRNP-associated protein Nan1|Schizosaccharomy... 25 8.6 >SPAPB1E7.12 |rps602|rps6-2, rps6|40S ribosomal protein S6|Schizosaccharomyces pombe|chr 1|||Manual Length = 239 Score = 132 bits (319), Expect = 4e-32 Identities = 65/98 (66%), Positives = 74/98 (75%) Frame = +2 Query: 11 RVRLLMSKGHSCYRPRRDGXRKRKSVRGCIXDANLSVLALVIVRKGAQEIPGLTDGNVPR 190 RVRLL+ GH CYRPRRDG RKRKSVRGCI +L+VLAL IV++G Q+IPGLTD VP+ Sbjct: 72 RVRLLLRAGHPCYRPRRDGERKRKSVRGCIVGQDLAVLALAIVKQGEQDIPGLTDVTVPK 131 Query: 191 RLGPKRASKIRKLFNLSKEDDDVVMSSNACSQPRKEKK 304 RLGPKRASKIR+ FNLSKEDD P+KE K Sbjct: 132 RLGPKRASKIRRFFNLSKEDDVRQFVIRREVVPKKEGK 169 Score = 56.0 bits (129), Expect = 4e-09 Identities = 35/83 (42%), Positives = 49/83 (59%), Gaps = 1/83 (1%) Frame = +1 Query: 262 YVVKR-VLPAKEGKENAKPRHKAPKIQRLVTPVVLQXXXXXXXXXXXXXXXXXSSEAEYA 438 +V++R V+P KEGK KP KAPKIQRLVTP LQ AE+A Sbjct: 156 FVIRREVVPKKEGK---KPYTKAPKIQRLVTPRTLQHKRHRFALKRRQAEKNREEAAEFA 212 Query: 439 KLLAQRKKESKVRRQEEIKRRRS 507 +L+A+R E+K +++E +K RR+ Sbjct: 213 QLMAKRVAEAK-QKKEVVKARRA 234 >SPAC13G6.07c |rps601|rps6-1|40S ribosomal protein S6|Schizosaccharomyces pombe|chr 1|||Manual Length = 239 Score = 132 bits (318), Expect = 5e-32 Identities = 64/98 (65%), Positives = 74/98 (75%) Frame = +2 Query: 11 RVRLLMSKGHSCYRPRRDGXRKRKSVRGCIXDANLSVLALVIVRKGAQEIPGLTDGNVPR 190 RVRLL+ GH CYRPRRDG RKRKSVRGCI +L+VLAL I+++G Q+IPGLTD VP+ Sbjct: 72 RVRLLLRAGHPCYRPRRDGERKRKSVRGCIVGQDLAVLALAIIKQGEQDIPGLTDVTVPK 131 Query: 191 RLGPKRASKIRKLFNLSKEDDDVVMSSNACSQPRKEKK 304 RLGPKRASKIR+ FNLSKEDD P+KE K Sbjct: 132 RLGPKRASKIRRFFNLSKEDDVRQFVIRREVVPKKEGK 169 Score = 56.4 bits (130), Expect = 3e-09 Identities = 36/83 (43%), Positives = 48/83 (57%), Gaps = 1/83 (1%) Frame = +1 Query: 262 YVVKR-VLPAKEGKENAKPRHKAPKIQRLVTPVVLQXXXXXXXXXXXXXXXXXSSEAEYA 438 +V++R V+P KEGK KP KAPKIQRLVTP LQ AE+A Sbjct: 156 FVIRREVVPKKEGK---KPYTKAPKIQRLVTPRTLQHKRHRFALKRRQAEKNREEAAEFA 212 Query: 439 KLLAQRKKESKVRRQEEIKRRRS 507 +L+A+R E+K +R E +K RR+ Sbjct: 213 QLMAKRVAEAKQKR-EVVKARRA 234 >SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1085 Score = 28.7 bits (61), Expect = 0.70 Identities = 15/49 (30%), Positives = 27/49 (55%) Frame = +1 Query: 424 EAEYAKLLAQRKKESKVRRQEEIKRRRSVQCVIPRALARVLRKSDLLYF 570 E E ++L +RK+ K+ ++EE +RRR + R LR++ + F Sbjct: 695 EEEEKRILEERKRREKLDKEEEERRRRELLEKESEEKERRLREAKIAAF 743 >SPBC31F10.13c |hip1|hir1|hira protein Hip1|Schizosaccharomyces pombe|chr 2|||Manual Length = 932 Score = 27.1 bits (57), Expect = 2.1 Identities = 16/46 (34%), Positives = 23/46 (50%) Frame = -3 Query: 256 VIIFFAKVEQLTDFGSTFGT*TAGYISISQSRNFLGTLAHNNKSQD 119 V+I + K E + GSTFG +G R++ L H+N QD Sbjct: 97 VVIIWHKEEAIPGLGSTFG---SGEKHTENWRSYRRLLGHDNDIQD 139 >SPCC1450.06c |grx3||monothiol glutaredoxin Grx3|Schizosaccharomyces pombe|chr 3|||Manual Length = 166 Score = 26.2 bits (55), Expect = 3.7 Identities = 19/62 (30%), Positives = 29/62 (46%), Gaps = 1/62 (1%) Frame = +2 Query: 143 KGAQEIP-GLTDGNVPRRLGPKRASKIRKLFNLSKEDDDVVMSSNACSQPRKEKKMLNPD 319 K A+ P GL+D +VP PK +K F + E+ ++ S C KK+L Sbjct: 32 KNAKAGPLGLSDVSVPS--APKLPAKDSTDFEVFLENPVIIFSRPGCPYSAAAKKLLTET 89 Query: 320 IR 325 +R Sbjct: 90 LR 91 >SPAC6F6.02c |pof5||F-box protein Pof5|Schizosaccharomyces pombe|chr 1|||Manual Length = 348 Score = 25.4 bits (53), Expect = 6.5 Identities = 11/37 (29%), Positives = 19/37 (51%) Frame = -1 Query: 525 WNHALN*PPAFDLFLATHLGFLLSLCKQFSIFSLRGL 415 W+H + +FD F A + G LL +C+ + L + Sbjct: 9 WHHIFDHLISFDKFEAKNFGGLLRICRSSYVGGLHAI 45 >SPCC576.10c |rpt3||19S proteasome regulatory subunit Rpt3|Schizosaccharomyces pombe|chr 3|||Manual Length = 389 Score = 25.0 bits (52), Expect = 8.6 Identities = 12/28 (42%), Positives = 17/28 (60%) Frame = +1 Query: 460 KESKVRRQEEIKRRRSVQCVIPRALARV 543 K +R QEE+KR +SV VI + L + Sbjct: 38 KRELIRAQEEVKRIQSVPLVIGQFLEAI 65 >SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 543 Score = 25.0 bits (52), Expect = 8.6 Identities = 12/30 (40%), Positives = 16/30 (53%), Gaps = 1/30 (3%) Frame = -2 Query: 365 CRTTGVTSLWILGALC-LGLAFSFPSLAGS 279 C G+ W +GA C L +AF+ LA S Sbjct: 76 CGAGGMVWSWFVGATCLLPIAFALSELASS 105 >SPAC1B3.13 |||U3 snoRNP-associated protein Nan1|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 25.0 bits (52), Expect = 8.6 Identities = 17/70 (24%), Positives = 34/70 (48%) Frame = +2 Query: 98 IXDANLSVLALVIVRKGAQEIPGLTDGNVPRRLGPKRASKIRKLFNLSKEDDDVVMSSNA 277 + D NL+ +A++ K + NVP++ +R LFN+ ++ +V + +A Sbjct: 179 LRDNNLATVAVITTDKAIFSL------NVPKKKRSQRWIHREHLFNMPQKLTNVSLCGSA 232 Query: 278 CSQPRKEKKM 307 C+ E K+ Sbjct: 233 CAVSDDEGKI 242 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,079,411 Number of Sequences: 5004 Number of extensions: 35640 Number of successful extensions: 87 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 82 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 87 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 267622334 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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