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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ps4M0390.Seq
         (936 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    27   5.0  
SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce...    26   8.8  

>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 26.6 bits (56), Expect = 5.0
 Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 5/63 (7%)
 Frame = +3

Query: 321 PIRPIVSRITIHWPSFYNVVTGKTLALPNLIALQ-----HIPLSPAGVIAKRPAPIALPN 485
           P  P+ S ++ H   +    +    +L N I+L      ++PLSP    A+ P+PI L +
Sbjct: 172 PRPPLPSSVSSHSSPYSTTSSTSLYSLYNDISLSCSPEPYLPLSPTRSPARTPSPIRLYS 231

Query: 486 SCA 494
           S A
Sbjct: 232 SDA 234


>SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1242

 Score = 25.8 bits (54), Expect = 8.8
 Identities = 9/22 (40%), Positives = 15/22 (68%)
 Frame = -3

Query: 133 TVFCYCCSLNSYLLQQCHRDQE 68
           + FC C  L+SY++ Q + D+E
Sbjct: 845 STFCRCLRLSSYVMGQRYSDEE 866


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,576,495
Number of Sequences: 5004
Number of extensions: 73043
Number of successful extensions: 138
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 134
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 138
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 475330268
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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