Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ps4M0268.Seq
         (886 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr 1|...    62   1e-10
SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr 3|||Ma...    34   0.031
SPAC17H9.11 |||cofilin/tropomyosin family protein|Schizosaccharo...    29   0.67 
SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces pom...    27   2.7  
SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun...    27   3.6  
SPAC3H5.08c |||WD repeat protein Wdr44 family|Schizosaccharomyce...    27   4.7  
SPBC21B10.03c |||ataxin-2 homolog|Schizosaccharomyces pombe|chr ...    27   4.7  

>SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 137

 Score = 61.7 bits (143), Expect = 1e-10
 Identities = 27/57 (47%), Positives = 38/57 (66%)
 Frame = +3

Query: 312 KQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEASQEAVEEKL 482
           + K+  +SW PD A +K KM+YSSS D L+++  G+   IQATD SE + E V EK+
Sbjct: 78  RNKIIFISWSPDVAPIKSKMVYSSSKDTLRRAFTGIGTDIQATDFSEVAYETVLEKV 134



 Score = 39.9 bits (89), Expect = 5e-04
 Identities = 21/54 (38%), Positives = 29/54 (53%)
 Frame = +1

Query: 64  SGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDL 225
           SGV VS  C   ++E+K  K  RYVVF + D K   V      + +++ FL DL
Sbjct: 4   SGVKVSPECLEAFQELKLGKSLRYVVFKMNDTKTEIVVEKKSTDKDFDTFLGDL 57


>SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 328

 Score = 33.9 bits (74), Expect = 0.031
 Identities = 15/27 (55%), Positives = 21/27 (77%)
 Frame = +3

Query: 306 SKKQKLFLMSWCPDTAKVKKKMLYSSS 386
           SKK  L L+S+ P+ A V++KMLY+SS
Sbjct: 76  SKKNLLQLISYVPENANVRRKMLYASS 102



 Score = 28.7 bits (61), Expect = 1.2
 Identities = 16/56 (28%), Positives = 32/56 (57%), Gaps = 5/56 (8%)
 Frame = +3

Query: 318 KLFLMSWCPDTAKVKKKMLYSSS----FDALKKSL-VGVQKYIQATDLSEASQEAV 470
           K+  +  CP  A VK +M+YSSS     D++K  L + +   I++ D ++ +++ +
Sbjct: 244 KILFIYICPMQATVKHRMVYSSSKLGLLDSIKAELGIVIDGKIESNDAADITEKEI 299


>SPAC17H9.11 |||cofilin/tropomyosin family
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 141

 Score = 29.5 bits (63), Expect = 0.67
 Identities = 17/63 (26%), Positives = 30/63 (47%)
 Frame = +3

Query: 294 TSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEASQEAVE 473
           T++      LF++ W P        M+Y+S+     + +  V K  +A D  + + EAV+
Sbjct: 79  TTDGRLSTPLFMIYWRPSATPNDLSMIYASA-KVWFQDVSQVHKVFEARDSEDITSEAVD 137

Query: 474 EKL 482
           E L
Sbjct: 138 EFL 140


>SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 447

 Score = 27.5 bits (58), Expect = 2.7
 Identities = 13/43 (30%), Positives = 22/43 (51%)
 Frame = +1

Query: 487 PPIANKQLYTRARDETEPALRHSCPDDTRPRHH*PLSIITKEN 615
           PP++ K     +    EP +RHS     RP+ H   S +T+++
Sbjct: 37  PPLSRKNPSNVSFWSNEPIIRHSSVKTDRPQFHRADSTVTEQS 79


>SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 214

 Score = 27.1 bits (57), Expect = 3.6
 Identities = 10/26 (38%), Positives = 19/26 (73%)
 Frame = +3

Query: 384 SFDALKKSLVGVQKYIQATDLSEASQ 461
           S +AL++ L G + Y+Q+TD++  S+
Sbjct: 188 SLEALQEELEGFEDYVQSTDIAAMSK 213


>SPAC3H5.08c |||WD repeat protein Wdr44 family|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 855

 Score = 26.6 bits (56), Expect = 4.7
 Identities = 12/44 (27%), Positives = 21/44 (47%)
 Frame = +2

Query: 461 GGRRREAPRHRSPINSFTHELATKPNPLSDTPALTTRGHDTTSR 592
           G    EA +  + I    HE+ T  +P+S+  A+     D +S+
Sbjct: 751 GATVNEAAKTAATIEQNEHEIQTSVDPISNVKAILPNADDVSSK 794


>SPBC21B10.03c |||ataxin-2 homolog|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 791

 Score = 26.6 bits (56), Expect = 4.7
 Identities = 14/52 (26%), Positives = 25/52 (48%)
 Frame = +2

Query: 434 SDRPLGSVSGGRRREAPRHRSPINSFTHELATKPNPLSDTPALTTRGHDTTS 589
           S R   S S G+R    ++ +  N+F+H  A+  + L+  P  T +    T+
Sbjct: 717 SQRSFNS-SNGKRSNVHKNNNASNTFSHSNASTSSSLNAAPNTTAKSSSQTA 767


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,123,607
Number of Sequences: 5004
Number of extensions: 60426
Number of successful extensions: 188
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 182
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 188
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 444486180
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)