BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ps4M0267.Seq (955 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit C... 145 7e-36 SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit ... 47 3e-06 SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit ... 42 1e-04 SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit C... 35 0.015 SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subu... 33 0.078 SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces po... 30 0.55 SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subuni... 30 0.55 SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit... 29 0.97 SPBC1289.04c |pob1||Boi family protein|Schizosaccharomyces pombe... 28 1.7 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 2.9 SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 27 2.9 SPCC1494.10 ||SPCC70.01|transcription factor |Schizosaccharomyce... 27 3.9 SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 27 5.1 SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 26 6.8 SPCC1827.05c |||nucleolar RNA-binding protein NIFK |Schizosaccha... 26 9.0 SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc... 26 9.0 >SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit Cct2|Schizosaccharomyces pombe|chr 1|||Manual Length = 527 Score = 145 bits (352), Expect = 7e-36 Identities = 64/86 (74%), Positives = 76/86 (88%) Frame = +1 Query: 256 YNYPEQLFADAGVMAIEHADFEGIERLGLVTGGEIVSTFDSPDKVKLGHCKLIEEVLIGD 435 YN+PEQLFADAG+M+IEHADF+GIERL LVTGGEI STFD P+ VKLGHCK IEE++IG+ Sbjct: 293 YNWPEQLFADAGIMSIEHADFDGIERLSLVTGGEIASTFDHPELVKLGHCKKIEEIIIGE 352 Query: 436 ESLIRFSGVALGSACTIVIRGATQQL 513 + +I+FSGV G ACTIV+RGAT QL Sbjct: 353 DKMIKFSGVEAGEACTIVLRGATHQL 378 Score = 121 bits (291), Expect = 2e-28 Identities = 57/84 (67%), Positives = 69/84 (82%) Frame = +2 Query: 2 SXLDEGFLLNKKVGVHQPKKVENANILIANTPMDTDKIKVFGSTIKVDSMAKIAELEVAE 181 S LDEGF+LNK +GV+ PK +ENANILIANT MDTDK+KVFG+ ++VD+ K+AELE AE Sbjct: 208 SFLDEGFILNKTIGVNCPKVMENANILIANTAMDTDKVKVFGARVRVDTTGKLAELERAE 267 Query: 182 KEKMKDKVNKILAHKCNVFINRQL 253 +EKMK KV KI +H N FINRQL Sbjct: 268 REKMKAKVEKIKSHNINCFINRQL 291 Score = 39.5 bits (88), Expect = 7e-04 Identities = 23/85 (27%), Positives = 39/85 (45%) Frame = +3 Query: 510 VIDEAERSLHDALCVLAATVKEPKSYVDEGLVKC*WXXXXXXXXXXXXXXXXXXXXXXXX 689 ++DE+ER++HDAL VL+ TV E + + G + Sbjct: 378 LLDESERAIHDALAVLSQTVAESRVTLGGGCAEMLMAKAVEEAATHEPGKKAVAVSAFAK 437 Query: 690 XXXXXXSAVADNAXYDHSDLIARLR 764 + +ADNA +D S+L+A+L+ Sbjct: 438 ALSQLPTILADNAGFDSSELVAQLK 462 >SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit Cct7|Schizosaccharomyces pombe|chr 2|||Manual Length = 558 Score = 47.2 bits (107), Expect = 3e-06 Identities = 24/80 (30%), Positives = 35/80 (43%) Frame = +1 Query: 271 QLFADAGVMAIEHADFEGIERLGLVTGGEIVSTFDSPDKVKLGHCKLIEEVLIGDESLIR 450 Q FAD + + + R+ GG I ST + ++ LG C EE IG + Sbjct: 306 QYFADRDIFCAGRVAADDLNRVVQAVGGSIQSTCSNIEEKHLGTCDTFEERQIGGDRFNL 365 Query: 451 FSGVALGSACTIVIRGATQQ 510 F G CT+++RG Q Sbjct: 366 FEGCPKAKTCTLILRGGADQ 385 >SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit Cct4|Schizosaccharomyces pombe|chr 2|||Manual Length = 527 Score = 42.3 bits (95), Expect = 1e-04 Identities = 23/71 (32%), Positives = 40/71 (56%), Gaps = 1/71 (1%) Frame = +1 Query: 292 VMAIEHADFEGIERLGLVTGGEIVSTFDSPDKVKLGHCKLIEEVLIGDESLIRFSGVA-L 468 +M I+ + + +E + TG + ++ +S + KLGH L+EE E +++FSGV Sbjct: 311 IMVIKDIERDEVEFICKSTGCKPIADIESFAEDKLGHADLVEETSSSGEKIVKFSGVKNA 370 Query: 469 GSACTIVIRGA 501 G +I+ RGA Sbjct: 371 GKTVSILCRGA 381 Score = 35.1 bits (77), Expect = 0.015 Identities = 14/21 (66%), Positives = 18/21 (85%) Frame = +3 Query: 513 IDEAERSLHDALCVLAATVKE 575 ++EAERSLHDALCV+ VK+ Sbjct: 386 LEEAERSLHDALCVIRCLVKQ 406 >SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit Cct6|Schizosaccharomyces pombe|chr 2|||Manual Length = 535 Score = 35.1 bits (77), Expect = 0.015 Identities = 22/75 (29%), Positives = 34/75 (45%) Frame = +1 Query: 274 LFADAGVMAIEHADFEGIERLGLVTGGEIVSTFDSPDKVKLGHCKLIEEVLIGDESLIRF 453 + A G+MA+ A +ERL L GG ++ D + LG + E +G+E Sbjct: 303 VLAKNGIMALRRAKRRNMERLQLACGGVAQNSVDDLNPEVLGWAGSVYERTLGEEKYTFV 362 Query: 454 SGVALGSACTIVIRG 498 V + TI+I G Sbjct: 363 EDVKDPKSATILIHG 377 Score = 28.7 bits (61), Expect = 1.3 Identities = 18/61 (29%), Positives = 34/61 (55%) Frame = +2 Query: 53 PKKVENANILIANTPMDTDKIKVFGSTIKVDSMAKIAELEVAEKEKMKDKVNKILAHKCN 232 PK+V+NA ILI N ++ +K ++ S + + L +E++ + +K+ KI+ K Sbjct: 220 PKQVKNAYILILNVSLEYEKSEI-NSGFFYSTSEQRERLVESERKFVDNKLRKIVELKKE 278 Query: 233 V 235 V Sbjct: 279 V 279 Score = 27.1 bits (57), Expect = 3.9 Identities = 11/22 (50%), Positives = 16/22 (72%) Frame = -3 Query: 575 FFYSSSKHAKRIME*TLSFVDN 510 FFYS+S+ +R++E FVDN Sbjct: 246 FFYSTSEQRERLVESERKFVDN 267 >SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subunit Cct5|Schizosaccharomyces pombe|chr 1|||Manual Length = 546 Score = 32.7 bits (71), Expect = 0.078 Identities = 11/22 (50%), Positives = 19/22 (86%) Frame = +3 Query: 510 VIDEAERSLHDALCVLAATVKE 575 ++DEA+R+LHDALCV+ +++ Sbjct: 396 IVDEAKRALHDALCVVRNLIRD 417 >SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 851 Score = 29.9 bits (64), Expect = 0.55 Identities = 19/67 (28%), Positives = 31/67 (46%) Frame = +2 Query: 44 VHQPKKVENANILIANTPMDTDKIKVFGSTIKVDSMAKIAELEVAEKEKMKDKVNKILAH 223 +H+ K E T K+KV+G + ++I+ L + KEK KD ++ H Sbjct: 616 IHKEAKSEIEKDSSKPTEDQESKLKVYGRV----AYSRISNLHKSSKEKGKDSQVRVALH 671 Query: 224 KCNVFIN 244 K N +N Sbjct: 672 KENTALN 678 >SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subunit Cct1|Schizosaccharomyces pombe|chr 2|||Manual Length = 556 Score = 29.9 bits (64), Expect = 0.55 Identities = 18/81 (22%), Positives = 37/81 (45%), Gaps = 6/81 (7%) Frame = +1 Query: 283 DAGVMAIEHADFEGIERLGLVTGGEIVSTF------DSPDKVKLGHCKLIEEVLIGDESL 444 +AG MA+ E + R+ +G ++S+ ++ + LG + + + D+ Sbjct: 307 EAGAMAVRRCKKEDLRRIAKASGATLLSSLSNLEGEETFESSYLGSAEEVVQEKFSDDEC 366 Query: 445 IRFSGVALGSACTIVIRGATQ 507 I G S+ +IV+RG + Sbjct: 367 ILVKGTKAYSSASIVLRGPNE 387 Score = 28.7 bits (61), Expect = 1.3 Identities = 13/32 (40%), Positives = 20/32 (62%) Frame = +3 Query: 513 IDEAERSLHDALCVLAATVKEPKSYVDEGLVK 608 +DE ERS+HD+L V+ T++ K G V+ Sbjct: 390 LDEMERSMHDSLSVVKRTLESGKVVPGGGAVE 421 >SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit Cct3|Schizosaccharomyces pombe|chr 2|||Manual Length = 528 Score = 29.1 bits (62), Expect = 0.97 Identities = 19/77 (24%), Positives = 33/77 (42%), Gaps = 1/77 (1%) Frame = +1 Query: 286 AGVMAIEHADFEGIERLGLVTGGEIVSTFDSPDKVKLGH-CKLIEEVLIGDESLIRFSGV 462 A + A+ R+ G IV+ + + +G C L +GDE +G Sbjct: 305 ANITALRRTRKSDNNRIARACGANIVNRLEDLREKDVGTGCGLFYIDKLGDEYYTFLTGC 364 Query: 463 ALGSACTIVIRGATQQL 513 ACTI++RG ++ + Sbjct: 365 KNPKACTILLRGPSKDI 381 >SPBC1289.04c |pob1||Boi family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 871 Score = 28.3 bits (60), Expect = 1.7 Identities = 26/75 (34%), Positives = 35/75 (46%), Gaps = 3/75 (4%) Frame = -3 Query: 497 PRITIVQAEPNATPEKRIKLSSPIRTSSINLQ*PSFTLSGESNVDT---ISPPVTKPSLS 327 P+IT + E ++ R+ S+ + SS S T S ES D S PVT +S Sbjct: 540 PKITTIDGESPSSISSRLPSSNLEQGSS-----SSVTKSPESMPDPSAKASSPVTSKGVS 594 Query: 326 IPSKSACSIAITPAS 282 I KSA + TP S Sbjct: 595 INEKSAVNNYATPLS 609 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 27.5 bits (58), Expect = 2.9 Identities = 23/77 (29%), Positives = 33/77 (42%), Gaps = 3/77 (3%) Frame = -3 Query: 500 APRITIVQAEPNATPEKRIKLSSPIRTSSINLQ*PSFTL-SGESNVDTIS--PPVTKPSL 330 AP + I + P S+P + + + PS SG V S PPV KPS+ Sbjct: 1040 APPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSV 1099 Query: 329 SIPSKSACSIAITPASA 279 + P S+A+ P A Sbjct: 1100 AAPPVPKPSVAVPPVPA 1116 >SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1436 Score = 27.5 bits (58), Expect = 2.9 Identities = 12/50 (24%), Positives = 27/50 (54%) Frame = -3 Query: 458 PEKRIKLSSPIRTSSINLQ*PSFTLSGESNVDTISPPVTKPSLSIPSKSA 309 P + + +S+P+ +I+ ++ +S + + PP + S+ IP K+A Sbjct: 100 PAEALTISTPVDPINIDELDRAYAVSPSDTSNLLHPPTSSSSIPIPIKNA 149 >SPCC1494.10 ||SPCC70.01|transcription factor |Schizosaccharomyces pombe|chr 3|||Manual Length = 964 Score = 27.1 bits (57), Expect = 3.9 Identities = 13/37 (35%), Positives = 18/37 (48%) Frame = +2 Query: 23 LLNKKVGVHQPKKVENANILIANTPMDTDKIKVFGST 133 L N P K E N++ AN + DK + FGS+ Sbjct: 108 LYNHMSSAPSPNKKEETNVVHANEDISLDKRQSFGSS 144 >SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces pombe|chr 2|||Manual Length = 726 Score = 26.6 bits (56), Expect = 5.1 Identities = 19/48 (39%), Positives = 24/48 (50%), Gaps = 1/48 (2%) Frame = -3 Query: 443 KLSSPIRTSSINLQ*PSFTLSGESNV-DTISPPVTKPSLSIPSKSACS 303 K S + +S ++ TLS S V T S PVT S S+ KSA S Sbjct: 194 KASKKLTSSPTSVASKKATLSSVSKVASTSSLPVTSVSASVDPKSAAS 241 >SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 3227 Score = 26.2 bits (55), Expect = 6.8 Identities = 12/37 (32%), Positives = 22/37 (59%), Gaps = 1/37 (2%) Frame = +1 Query: 721 TMLATTILISLLD-SEXHQFSXGGPRNXFRPXSESYY 828 +++ + +L L+D +E QFS P + F P ++S Y Sbjct: 1552 SVIVSFLLDELMDLTETRQFSDRSPNSEFTPENDSLY 1588 >SPCC1827.05c |||nucleolar RNA-binding protein NIFK |Schizosaccharomyces pombe|chr 3|||Manual Length = 276 Score = 25.8 bits (54), Expect = 9.0 Identities = 16/40 (40%), Positives = 21/40 (52%), Gaps = 1/40 (2%) Frame = +2 Query: 110 KIKVFGSTIKVDSMAKIAELEVAEKEKMKDKVNKIL-AHK 226 K+K G T++ D A VA K+ K K K+L AHK Sbjct: 237 KLKELGITLESDVSHPKAASPVASKKSSKKKNKKVLAAHK 276 >SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 498 Score = 25.8 bits (54), Expect = 9.0 Identities = 12/30 (40%), Positives = 18/30 (60%) Frame = +1 Query: 442 LIRFSGVALGSACTIVIRGATQQLSTKLSV 531 + R G AL + CT+VIR A + T L++ Sbjct: 176 ITRKVGAALAAGCTVVIRPAAETPFTALAL 205 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,284,549 Number of Sequences: 5004 Number of extensions: 59645 Number of successful extensions: 228 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 199 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 227 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 487313384 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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