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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ps4M0267.Seq
         (955 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit C...   145   7e-36
SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit ...    47   3e-06
SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit ...    42   1e-04
SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit C...    35   0.015
SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subu...    33   0.078
SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces po...    30   0.55 
SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subuni...    30   0.55 
SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit...    29   0.97 
SPBC1289.04c |pob1||Boi family protein|Schizosaccharomyces pombe...    28   1.7  
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual    27   2.9  
SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki...    27   2.9  
SPCC1494.10 ||SPCC70.01|transcription factor |Schizosaccharomyce...    27   3.9  
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ...    27   5.1  
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||...    26   6.8  
SPCC1827.05c |||nucleolar RNA-binding protein NIFK |Schizosaccha...    26   9.0  
SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc...    26   9.0  

>SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit
           Cct2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 527

 Score =  145 bits (352), Expect = 7e-36
 Identities = 64/86 (74%), Positives = 76/86 (88%)
 Frame = +1

Query: 256 YNYPEQLFADAGVMAIEHADFEGIERLGLVTGGEIVSTFDSPDKVKLGHCKLIEEVLIGD 435
           YN+PEQLFADAG+M+IEHADF+GIERL LVTGGEI STFD P+ VKLGHCK IEE++IG+
Sbjct: 293 YNWPEQLFADAGIMSIEHADFDGIERLSLVTGGEIASTFDHPELVKLGHCKKIEEIIIGE 352

Query: 436 ESLIRFSGVALGSACTIVIRGATQQL 513
           + +I+FSGV  G ACTIV+RGAT QL
Sbjct: 353 DKMIKFSGVEAGEACTIVLRGATHQL 378



 Score =  121 bits (291), Expect = 2e-28
 Identities = 57/84 (67%), Positives = 69/84 (82%)
 Frame = +2

Query: 2   SXLDEGFLLNKKVGVHQPKKVENANILIANTPMDTDKIKVFGSTIKVDSMAKIAELEVAE 181
           S LDEGF+LNK +GV+ PK +ENANILIANT MDTDK+KVFG+ ++VD+  K+AELE AE
Sbjct: 208 SFLDEGFILNKTIGVNCPKVMENANILIANTAMDTDKVKVFGARVRVDTTGKLAELERAE 267

Query: 182 KEKMKDKVNKILAHKCNVFINRQL 253
           +EKMK KV KI +H  N FINRQL
Sbjct: 268 REKMKAKVEKIKSHNINCFINRQL 291



 Score = 39.5 bits (88), Expect = 7e-04
 Identities = 23/85 (27%), Positives = 39/85 (45%)
 Frame = +3

Query: 510 VIDEAERSLHDALCVLAATVKEPKSYVDEGLVKC*WXXXXXXXXXXXXXXXXXXXXXXXX 689
           ++DE+ER++HDAL VL+ TV E +  +  G  +                           
Sbjct: 378 LLDESERAIHDALAVLSQTVAESRVTLGGGCAEMLMAKAVEEAATHEPGKKAVAVSAFAK 437

Query: 690 XXXXXXSAVADNAXYDHSDLIARLR 764
                 + +ADNA +D S+L+A+L+
Sbjct: 438 ALSQLPTILADNAGFDSSELVAQLK 462


>SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit
           Cct7|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 558

 Score = 47.2 bits (107), Expect = 3e-06
 Identities = 24/80 (30%), Positives = 35/80 (43%)
 Frame = +1

Query: 271 QLFADAGVMAIEHADFEGIERLGLVTGGEIVSTFDSPDKVKLGHCKLIEEVLIGDESLIR 450
           Q FAD  +        + + R+    GG I ST  + ++  LG C   EE  IG +    
Sbjct: 306 QYFADRDIFCAGRVAADDLNRVVQAVGGSIQSTCSNIEEKHLGTCDTFEERQIGGDRFNL 365

Query: 451 FSGVALGSACTIVIRGATQQ 510
           F G      CT+++RG   Q
Sbjct: 366 FEGCPKAKTCTLILRGGADQ 385


>SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit
           Cct4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 527

 Score = 42.3 bits (95), Expect = 1e-04
 Identities = 23/71 (32%), Positives = 40/71 (56%), Gaps = 1/71 (1%)
 Frame = +1

Query: 292 VMAIEHADFEGIERLGLVTGGEIVSTFDSPDKVKLGHCKLIEEVLIGDESLIRFSGVA-L 468
           +M I+  + + +E +   TG + ++  +S  + KLGH  L+EE     E +++FSGV   
Sbjct: 311 IMVIKDIERDEVEFICKSTGCKPIADIESFAEDKLGHADLVEETSSSGEKIVKFSGVKNA 370

Query: 469 GSACTIVIRGA 501
           G   +I+ RGA
Sbjct: 371 GKTVSILCRGA 381



 Score = 35.1 bits (77), Expect = 0.015
 Identities = 14/21 (66%), Positives = 18/21 (85%)
 Frame = +3

Query: 513 IDEAERSLHDALCVLAATVKE 575
           ++EAERSLHDALCV+   VK+
Sbjct: 386 LEEAERSLHDALCVIRCLVKQ 406


>SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit
           Cct6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 535

 Score = 35.1 bits (77), Expect = 0.015
 Identities = 22/75 (29%), Positives = 34/75 (45%)
 Frame = +1

Query: 274 LFADAGVMAIEHADFEGIERLGLVTGGEIVSTFDSPDKVKLGHCKLIEEVLIGDESLIRF 453
           + A  G+MA+  A    +ERL L  GG   ++ D  +   LG    + E  +G+E     
Sbjct: 303 VLAKNGIMALRRAKRRNMERLQLACGGVAQNSVDDLNPEVLGWAGSVYERTLGEEKYTFV 362

Query: 454 SGVALGSACTIVIRG 498
             V    + TI+I G
Sbjct: 363 EDVKDPKSATILIHG 377



 Score = 28.7 bits (61), Expect = 1.3
 Identities = 18/61 (29%), Positives = 34/61 (55%)
 Frame = +2

Query: 53  PKKVENANILIANTPMDTDKIKVFGSTIKVDSMAKIAELEVAEKEKMKDKVNKILAHKCN 232
           PK+V+NA ILI N  ++ +K ++  S     +  +   L  +E++ + +K+ KI+  K  
Sbjct: 220 PKQVKNAYILILNVSLEYEKSEI-NSGFFYSTSEQRERLVESERKFVDNKLRKIVELKKE 278

Query: 233 V 235
           V
Sbjct: 279 V 279



 Score = 27.1 bits (57), Expect = 3.9
 Identities = 11/22 (50%), Positives = 16/22 (72%)
 Frame = -3

Query: 575 FFYSSSKHAKRIME*TLSFVDN 510
           FFYS+S+  +R++E    FVDN
Sbjct: 246 FFYSTSEQRERLVESERKFVDN 267


>SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subunit
           Cct5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 546

 Score = 32.7 bits (71), Expect = 0.078
 Identities = 11/22 (50%), Positives = 19/22 (86%)
 Frame = +3

Query: 510 VIDEAERSLHDALCVLAATVKE 575
           ++DEA+R+LHDALCV+   +++
Sbjct: 396 IVDEAKRALHDALCVVRNLIRD 417


>SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 851

 Score = 29.9 bits (64), Expect = 0.55
 Identities = 19/67 (28%), Positives = 31/67 (46%)
 Frame = +2

Query: 44  VHQPKKVENANILIANTPMDTDKIKVFGSTIKVDSMAKIAELEVAEKEKMKDKVNKILAH 223
           +H+  K E        T     K+KV+G      + ++I+ L  + KEK KD   ++  H
Sbjct: 616 IHKEAKSEIEKDSSKPTEDQESKLKVYGRV----AYSRISNLHKSSKEKGKDSQVRVALH 671

Query: 224 KCNVFIN 244
           K N  +N
Sbjct: 672 KENTALN 678


>SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subunit
           Cct1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 556

 Score = 29.9 bits (64), Expect = 0.55
 Identities = 18/81 (22%), Positives = 37/81 (45%), Gaps = 6/81 (7%)
 Frame = +1

Query: 283 DAGVMAIEHADFEGIERLGLVTGGEIVSTF------DSPDKVKLGHCKLIEEVLIGDESL 444
           +AG MA+     E + R+   +G  ++S+       ++ +   LG  + + +    D+  
Sbjct: 307 EAGAMAVRRCKKEDLRRIAKASGATLLSSLSNLEGEETFESSYLGSAEEVVQEKFSDDEC 366

Query: 445 IRFSGVALGSACTIVIRGATQ 507
           I   G    S+ +IV+RG  +
Sbjct: 367 ILVKGTKAYSSASIVLRGPNE 387



 Score = 28.7 bits (61), Expect = 1.3
 Identities = 13/32 (40%), Positives = 20/32 (62%)
 Frame = +3

Query: 513 IDEAERSLHDALCVLAATVKEPKSYVDEGLVK 608
           +DE ERS+HD+L V+  T++  K     G V+
Sbjct: 390 LDEMERSMHDSLSVVKRTLESGKVVPGGGAVE 421


>SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit
           Cct3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 528

 Score = 29.1 bits (62), Expect = 0.97
 Identities = 19/77 (24%), Positives = 33/77 (42%), Gaps = 1/77 (1%)
 Frame = +1

Query: 286 AGVMAIEHADFEGIERLGLVTGGEIVSTFDSPDKVKLGH-CKLIEEVLIGDESLIRFSGV 462
           A + A+         R+    G  IV+  +   +  +G  C L     +GDE     +G 
Sbjct: 305 ANITALRRTRKSDNNRIARACGANIVNRLEDLREKDVGTGCGLFYIDKLGDEYYTFLTGC 364

Query: 463 ALGSACTIVIRGATQQL 513
               ACTI++RG ++ +
Sbjct: 365 KNPKACTILLRGPSKDI 381


>SPBC1289.04c |pob1||Boi family protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 871

 Score = 28.3 bits (60), Expect = 1.7
 Identities = 26/75 (34%), Positives = 35/75 (46%), Gaps = 3/75 (4%)
 Frame = -3

Query: 497 PRITIVQAEPNATPEKRIKLSSPIRTSSINLQ*PSFTLSGESNVDT---ISPPVTKPSLS 327
           P+IT +  E  ++   R+  S+  + SS      S T S ES  D     S PVT   +S
Sbjct: 540 PKITTIDGESPSSISSRLPSSNLEQGSS-----SSVTKSPESMPDPSAKASSPVTSKGVS 594

Query: 326 IPSKSACSIAITPAS 282
           I  KSA +   TP S
Sbjct: 595 INEKSAVNNYATPLS 609


>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1611

 Score = 27.5 bits (58), Expect = 2.9
 Identities = 23/77 (29%), Positives = 33/77 (42%), Gaps = 3/77 (3%)
 Frame = -3

Query: 500  APRITIVQAEPNATPEKRIKLSSPIRTSSINLQ*PSFTL-SGESNVDTIS--PPVTKPSL 330
            AP + I  + P          S+P    + + + PS    SG   V   S  PPV KPS+
Sbjct: 1040 APPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSV 1099

Query: 329  SIPSKSACSIAITPASA 279
            + P     S+A+ P  A
Sbjct: 1100 AAPPVPKPSVAVPPVPA 1116


>SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase
           kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1436

 Score = 27.5 bits (58), Expect = 2.9
 Identities = 12/50 (24%), Positives = 27/50 (54%)
 Frame = -3

Query: 458 PEKRIKLSSPIRTSSINLQ*PSFTLSGESNVDTISPPVTKPSLSIPSKSA 309
           P + + +S+P+   +I+    ++ +S     + + PP +  S+ IP K+A
Sbjct: 100 PAEALTISTPVDPINIDELDRAYAVSPSDTSNLLHPPTSSSSIPIPIKNA 149


>SPCC1494.10 ||SPCC70.01|transcription factor |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 964

 Score = 27.1 bits (57), Expect = 3.9
 Identities = 13/37 (35%), Positives = 18/37 (48%)
 Frame = +2

Query: 23  LLNKKVGVHQPKKVENANILIANTPMDTDKIKVFGST 133
           L N       P K E  N++ AN  +  DK + FGS+
Sbjct: 108 LYNHMSSAPSPNKKEETNVVHANEDISLDKRQSFGSS 144


>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 726

 Score = 26.6 bits (56), Expect = 5.1
 Identities = 19/48 (39%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
 Frame = -3

Query: 443 KLSSPIRTSSINLQ*PSFTLSGESNV-DTISPPVTKPSLSIPSKSACS 303
           K S  + +S  ++     TLS  S V  T S PVT  S S+  KSA S
Sbjct: 194 KASKKLTSSPTSVASKKATLSSVSKVASTSSLPVTSVSASVDPKSAAS 241


>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 3227

 Score = 26.2 bits (55), Expect = 6.8
 Identities = 12/37 (32%), Positives = 22/37 (59%), Gaps = 1/37 (2%)
 Frame = +1

Query: 721  TMLATTILISLLD-SEXHQFSXGGPRNXFRPXSESYY 828
            +++ + +L  L+D +E  QFS   P + F P ++S Y
Sbjct: 1552 SVIVSFLLDELMDLTETRQFSDRSPNSEFTPENDSLY 1588


>SPCC1827.05c |||nucleolar RNA-binding protein NIFK
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 276

 Score = 25.8 bits (54), Expect = 9.0
 Identities = 16/40 (40%), Positives = 21/40 (52%), Gaps = 1/40 (2%)
 Frame = +2

Query: 110 KIKVFGSTIKVDSMAKIAELEVAEKEKMKDKVNKIL-AHK 226
           K+K  G T++ D     A   VA K+  K K  K+L AHK
Sbjct: 237 KLKELGITLESDVSHPKAASPVASKKSSKKKNKKVLAAHK 276


>SPAC1002.12c |||succinate-semialdehyde dehydrogenase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 498

 Score = 25.8 bits (54), Expect = 9.0
 Identities = 12/30 (40%), Positives = 18/30 (60%)
 Frame = +1

Query: 442 LIRFSGVALGSACTIVIRGATQQLSTKLSV 531
           + R  G AL + CT+VIR A +   T L++
Sbjct: 176 ITRKVGAALAAGCTVVIRPAAETPFTALAL 205


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,284,549
Number of Sequences: 5004
Number of extensions: 59645
Number of successful extensions: 228
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 199
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 227
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 487313384
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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