Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ps4M0258.Seq
(900 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g13950.1 68414.m01639 eukaryotic translation initiation facto... 105 4e-23
At1g26630.1 68414.m03243 eukaryotic translation initiation facto... 103 1e-22
At1g69410.1 68414.m07972 eukaryotic translation initiation facto... 103 2e-22
At2g33255.1 68415.m04075 haloacid dehalogenase-like hydrolase fa... 29 3.2
At5g14230.1 68418.m01663 ankyrin repeat family protein contains ... 29 4.2
At5g03140.1 68418.m00262 lectin protein kinase family protein co... 29 5.6
At3g51290.1 68416.m05614 proline-rich family protein 28 7.3
At2g13820.1 68415.m01526 protease inhibitor/seed storage/lipid t... 28 7.3
At3g06450.1 68416.m00746 anion exchange family protein similar t... 28 9.7
>At1g13950.1 68414.m01639 eukaryotic translation initiation factor
5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Arabidopsis thaliana}
Length = 158
Score = 105 bits (252), Expect = 4e-23
Identities = 43/72 (59%), Positives = 56/72 (77%)
Frame = +1
Query: 64 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 243
E+ HFE+ D+GAS T+P Q +RKNG++++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 EEHHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 244 IFNGKSMKISVP 279
IF K ++ VP
Sbjct: 64 IFTSKKLEDIVP 75
Score = 105 bits (252), Expect = 4e-23
Identities = 47/86 (54%), Positives = 66/86 (76%), Gaps = 1/86 (1%)
Frame = +3
Query: 255 KKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGD-LGTQLR 431
KK EDI PS+HN DVPHV R DYQL DIS+DGY++L+ DNG ++DLK+P+ D L Q++
Sbjct: 68 KKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLLQQIK 127
Query: 432 TDFDSGKELLCTVLKSCGEECVIAVK 509
+ FD GK+L+ +V+ + GEE + A+K
Sbjct: 128 SGFDDGKDLVVSVMSAMGEEQINALK 153
>At1g26630.1 68414.m03243 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similariy to
SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1
(eIF-5A 1) {Lycopersicon esculentum}
Length = 159
Score = 103 bits (248), Expect = 1e-22
Identities = 46/87 (52%), Positives = 65/87 (74%), Gaps = 1/87 (1%)
Frame = +3
Query: 255 KKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGTQLR 431
KK EDI PS+HN DVPHV R DYQL DI++DG+++L+ D+G ++DLK+P D L Q+R
Sbjct: 68 KKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLTAQMR 127
Query: 432 TDFDSGKELLCTVLKSCGEECVIAVKQ 512
FD GK+++ +V+ S GEE + AVK+
Sbjct: 128 LGFDEGKDIVVSVMSSMGEEQICAVKE 154
Score = 96.7 bits (230), Expect = 2e-20
Identities = 40/72 (55%), Positives = 52/72 (72%)
Frame = +1
Query: 64 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 243
++ HFE +SGAS T+P +RK G +++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 DEHHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 244 IFNGKSMKISVP 279
IF K ++ VP
Sbjct: 64 IFTAKKLEDIVP 75
>At1g69410.1 68414.m07972 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similarity to
eukaryotic initiation factor 5A (2) (Nicotiana
plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Lycopersicon esculentum}
Length = 158
Score = 103 bits (246), Expect = 2e-22
Identities = 46/87 (52%), Positives = 66/87 (75%), Gaps = 1/87 (1%)
Frame = +3
Query: 255 KKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGTQLR 431
KK EDI PS+HN DVPHV R DYQL DIS+DG+++L+ DNG ++DLK+P D L TQL+
Sbjct: 68 KKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQLK 127
Query: 432 TDFDSGKELLCTVLKSCGEECVIAVKQ 512
F+ GK+++ +V+ + GEE + A+K+
Sbjct: 128 NGFEEGKDIVVSVMSAMGEEQMCALKE 154
Score = 101 bits (243), Expect = 5e-22
Identities = 42/72 (58%), Positives = 55/72 (76%)
Frame = +1
Query: 64 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 243
++ HFE+ D+GAS T+P Q +RK G +++KGRPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 DEHHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 244 IFNGKSMKISVP 279
IF K ++ VP
Sbjct: 64 IFTSKKLEDIVP 75
>At2g33255.1 68415.m04075 haloacid dehalogenase-like hydrolase
family protein contains InterPro accession IPR005834:
Haloacid dehalogenase-like hydrolase
Length = 224
Score = 29.5 bits (63), Expect = 3.2
Identities = 16/40 (40%), Positives = 20/40 (50%)
Frame = -1
Query: 345 HPRYQSVGSLRASREVRPCCVWRDRYLHTFSIEDINPNQV 226
H R++ + S RE RP D LH S DI PN+V
Sbjct: 121 HQRFEVIFSPALGREFRPYKPNPDPLLHICSTWDIQPNEV 160
>At5g14230.1 68418.m01663 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 591
Score = 29.1 bits (62), Expect = 4.2
Identities = 15/34 (44%), Positives = 16/34 (47%)
Frame = +3
Query: 393 LKIPDGDLGTQLRTDFDSGKELLCTVLKSCGEEC 494
L IPDGD T L G +C L SCG C
Sbjct: 412 LDIPDGDGYTPLMLAAREGHGHMCEYLISCGANC 445
>At5g03140.1 68418.m00262 lectin protein kinase family protein
contains Pfam domains, PF00138: Legume lectins alpha
domain, PF00139: Legume lectins beta domain and PF00069:
Protein kinase domain
Length = 711
Score = 28.7 bits (61), Expect = 5.6
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = -2
Query: 164 RPFSITKPFLRRAEHCMGKVAEAPESPVSKCVSSMS 57
+P +T+P +R + A+ PE P++K SSMS
Sbjct: 631 QPDPVTRPTMRSVVQILVGEADVPEVPIAKPSSSMS 666
>At3g51290.1 68416.m05614 proline-rich family protein
Length = 602
Score = 28.3 bits (60), Expect = 7.3
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = +2
Query: 41 KTQQWVTSKTHTLRPETPGPQPPSP 115
+T W T+ T ++ P P P PP P
Sbjct: 91 ETTTWTTTTTSSVLPPPPPPPPPPP 115
>At2g13820.1 68415.m01526 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein contains Pfam
protease inhibitor/seed storage/LTP family domain
PF00234
Length = 169
Score = 28.3 bits (60), Expect = 7.3
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = +2
Query: 656 GARKTAGXPSPSLTSFANTSTPSISP 733
G TAG +P+L+S AN +TP SP
Sbjct: 119 GLSPTAGAGAPALSSGANAATPVSSP 144
>At3g06450.1 68416.m00746 anion exchange family protein similar to
putative Anion exchanger family members: GB:AAD39673,
GB:AAD55295 [Arabidopsis thaliana]
Length = 732
Score = 27.9 bits (59), Expect = 9.7
Identities = 11/20 (55%), Positives = 11/20 (55%)
Frame = -1
Query: 663 RAPPLYXYSFFKLNLYTILC 604
R PP Y Y F L TILC
Sbjct: 329 RKPPAYHYDLFLLGFLTILC 348
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,667,699
Number of Sequences: 28952
Number of extensions: 393849
Number of successful extensions: 1104
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 1028
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1101
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2120147664
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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