Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ps4M0196.Seq
(822 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g11150.1 68417.m01807 vacuolar ATP synthase subunit E / V-ATP... 72 4e-13
At3g08560.1 68416.m00993 vacuolar ATP synthase subunit E, putati... 69 5e-12
At1g64200.1 68414.m07273 vacuolar ATP synthase subunit E, putati... 60 1e-09
At3g02260.1 68416.m00207 auxin transport protein (BIG) nearly id... 29 2.8
At3g19050.1 68416.m02420 kinesin motor protein-related contains ... 29 3.7
At3g03790.2 68416.m00389 ankyrin repeat family protein / regulat... 29 4.9
At3g03790.1 68416.m00388 ankyrin repeat family protein / regulat... 29 4.9
At3g23160.1 68416.m02919 expressed protein contains Pfam domain ... 28 6.5
At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N) f... 28 6.5
At3g10040.1 68416.m01204 expressed protein est match 28 8.6
>At4g11150.1 68417.m01807 vacuolar ATP synthase subunit E / V-ATPase
E subunit / vacuolar proton pump E subunit (VATE)
identical to SP|Q39258 Vacuolar ATP synthase subunit E
(EC 3.6.3.14) (V-ATPase E subunit) (Vacuolar proton pump
E subunit) {Arabidopsis thaliana}
Length = 230
Score = 72.1 bits (169), Expect = 4e-13
Identities = 38/86 (44%), Positives = 54/86 (62%)
Frame = +2
Query: 257 KKIQSSNMLNQARLKVLKVREDHVRNVLDEAXKRLAEVPKDTKLYSELLVTLIVQALFQL 436
KKI S LN +R+KVL+ ++D V + D+A K L V +D Y +LL LIVQ L +L
Sbjct: 66 KKIDYSMQLNASRIKVLQAQDDIVNAMKDQAAKDLLNVSRDEYAYKQLLKDLIVQCLLRL 125
Query: 437 MEPTVTIRVRQTDKALVESLLGKANK 514
EP+V +R R+ D LVE++L A +
Sbjct: 126 KEPSVLLRCREEDLGLVEAVLDDAKE 151
Score = 54.4 bits (125), Expect = 9e-08
Identities = 26/67 (38%), Positives = 40/67 (59%)
Frame = +3
Query: 63 LSDADVQKQIKHMMAFIEQXXXXXXXXXXXXXXXXFNIEKGRLVQQQRLKIMEYYEKKEK 242
++D DV +QI+ M+ FI Q FNIEK +LV+ ++ KI + YEKKEK
Sbjct: 1 MNDGDVSRQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQDYEKKEK 60
Query: 243 QVELRRR 263
Q ++R++
Sbjct: 61 QADVRKK 67
>At3g08560.1 68416.m00993 vacuolar ATP synthase subunit E, putative
/ V-ATPase E subunit, putative / vacuolar proton pump E
subunit, putative similar to SP|Q39258 Vacuolar ATP
synthase subunit E (EC 3.6.3.14) (V-ATPase E subunit)
(Vacuolar proton pump E subunit) {Arabidopsis thaliana};
contains Pfam profile PF01991: ATP synthase (E/31 kDa)
subunit
Length = 235
Score = 68.5 bits (160), Expect = 5e-12
Identities = 34/86 (39%), Positives = 54/86 (62%)
Frame = +2
Query: 257 KKIQSSNMLNQARLKVLKVREDHVRNVLDEAXKRLAEVPKDTKLYSELLVTLIVQALFQL 436
K+I S LN +R+K L+ ++D V + D A K L V D Y +LL +LI+++L +L
Sbjct: 66 KRIDYSTQLNASRIKYLQAQDDVVTAMKDSAAKDLLRVSNDKNNYKKLLKSLIIESLLRL 125
Query: 437 MEPTVTIRVRQTDKALVESLLGKANK 514
EP+V +R R+ DK +VES++ A +
Sbjct: 126 KEPSVLLRCREMDKKVVESVIEDAKR 151
Score = 51.6 bits (118), Expect = 6e-07
Identities = 25/72 (34%), Positives = 42/72 (58%)
Frame = +3
Query: 63 LSDADVQKQIKHMMAFIEQXXXXXXXXXXXXXXXXFNIEKGRLVQQQRLKIMEYYEKKEK 242
++DADV KQI+ M+ FI Q FNIE+ +L++ + K+ + Y++K K
Sbjct: 1 MNDADVSKQIQQMVRFIRQEAEEKANEISISAEEEFNIERLQLLESAKRKLRQDYDRKLK 60
Query: 243 QVELRRRSNLRT 278
QV++R+R + T
Sbjct: 61 QVDIRKRIDYST 72
>At1g64200.1 68414.m07273 vacuolar ATP synthase subunit E, putative
/ V-ATPase E subunit, putative / vacuolar proton pump E
subunit, putative similar to SP|Q39258 Vacuolar ATP
synthase subunit E (EC 3.6.3.14) (V-ATPase E subunit)
(Vacuolar proton pump E subunit) {Arabidopsis thaliana};
contains Pfam profile PF01991: ATP synthase (E/31 kDa)
subunit
Length = 237
Score = 60.5 bits (140), Expect = 1e-09
Identities = 37/92 (40%), Positives = 54/92 (58%), Gaps = 6/92 (6%)
Frame = +2
Query: 257 KKIQSSNMLNQARLKVLKVREDHVRNVLDEAXKRLAEVPKDTKL------YSELLVTLIV 418
KKI S LN +R+KVL+ ++D V + +EA K+L +V + Y LL LIV
Sbjct: 66 KKIDYSMQLNASRIKVLQAQDDIVNAMKEEAAKQLLKVSQHGFFNHHHHQYKHLLKDLIV 125
Query: 419 QALFQLMEPTVTIRVRQTDKALVESLLGKANK 514
Q L +L EP V +R R+ D +VES+L A++
Sbjct: 126 QCLLRLKEPAVLLRCREEDLDIVESMLDDASE 157
Score = 54.4 bits (125), Expect = 9e-08
Identities = 27/67 (40%), Positives = 40/67 (59%)
Frame = +3
Query: 63 LSDADVQKQIKHMMAFIEQXXXXXXXXXXXXXXXXFNIEKGRLVQQQRLKIMEYYEKKEK 242
++DAD QI+ M+ FI Q FNIEK +LV+ ++ KI + YEKKEK
Sbjct: 1 MNDADASIQIQQMVRFIRQEAEEKANEISISSEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60
Query: 243 QVELRRR 263
QV++R++
Sbjct: 61 QVDVRKK 67
>At3g02260.1 68416.m00207 auxin transport protein (BIG) nearly
identical to auxin transport protein; BIG [Arabidopsis
thaliana] GI:21779966; contains Pfam profiles PF02207:
Putative zinc finger in N-recognin, PF00569: Zinc finger
ZZ type
Length = 5098
Score = 29.5 bits (63), Expect = 2.8
Identities = 13/44 (29%), Positives = 21/44 (47%), Gaps = 1/44 (2%)
Frame = +1
Query: 373 KGHQTVLRAAGHTYC-AGSLPAHGTHCHHPRPSNRQGSGGVPAR 501
+GH+ V + +C G+ G+ C +P G+G PAR
Sbjct: 1613 RGHRVVYSRSSRFFCDCGAGGVRGSSCQCLKPRKYNGNGSAPAR 1656
>At3g19050.1 68416.m02420 kinesin motor protein-related contains
Pfam profile: PF00225 Kinesin motor domain; contains
non-consensus splice site (GC) at intron 12
Length = 2722
Score = 29.1 bits (62), Expect = 3.7
Identities = 15/36 (41%), Positives = 23/36 (63%)
Frame = +3
Query: 168 FNIEKGRLVQQQRLKIMEYYEKKEKQVELRRRSNLR 275
F +E RL+ Q R + E+YE+ E+++ L SNLR
Sbjct: 713 FALENIRLLDQLR-RFQEFYEEGEREILLGEVSNLR 747
>At3g03790.2 68416.m00389 ankyrin repeat family protein / regulator
of chromosome condensation (RCC1) family protein similar
to hect domain and RLD 2 GB:NP_004658 [Homo sapiens];
contains Pfam PF00415: Regulator of chromosome
condensation (RCC1); contains Pfam PF00023: Ankyrin
repeat; similar to rjs (GI:3414809) [Mus musculus];
similar to HERC2 (GI:4079809) [Homo sapiens]
Length = 1081
Score = 28.7 bits (61), Expect = 4.9
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +2
Query: 5 ALYRPFFVLVVLKISSSHGAQRCRCSETDQAHDG 106
+LY P + +VLK S + A +CR E ++ +G
Sbjct: 508 SLYHPAYAPIVLKKSQTLQADKCREEENEELDEG 541
>At3g03790.1 68416.m00388 ankyrin repeat family protein / regulator
of chromosome condensation (RCC1) family protein similar
to hect domain and RLD 2 GB:NP_004658 [Homo sapiens];
contains Pfam PF00415: Regulator of chromosome
condensation (RCC1); contains Pfam PF00023: Ankyrin
repeat; similar to rjs (GI:3414809) [Mus musculus];
similar to HERC2 (GI:4079809) [Homo sapiens]
Length = 1078
Score = 28.7 bits (61), Expect = 4.9
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +2
Query: 5 ALYRPFFVLVVLKISSSHGAQRCRCSETDQAHDG 106
+LY P + +VLK S + A +CR E ++ +G
Sbjct: 505 SLYHPAYAPIVLKKSQTLQADKCREEENEELDEG 538
>At3g23160.1 68416.m02919 expressed protein contains Pfam domain
PF05003: protein of unknown function (DUF668);
expression supported by MPSS
Length = 522
Score = 28.3 bits (60), Expect = 6.5
Identities = 16/33 (48%), Positives = 19/33 (57%)
Frame = -3
Query: 730 FYGLGLRPKEGVTLSPAAAAGRISSKRDSRSVA 632
F GLGLR K+ VTL + S +SRSVA
Sbjct: 235 FGGLGLRGKKDVTLKRDRSKNEASKAVNSRSVA 267
>At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N)
family protein similar to SP|Q9RHV9 Lysyl-tRNA
synthetase (EC 6.1.1.6) (Lysine--tRNA ligase) {Bacillus
stearothermophilus}; contains Pfam profile: PF00152 tRNA
synthetases class II (D, K and N)
Length = 602
Score = 28.3 bits (60), Expect = 6.5
Identities = 16/50 (32%), Positives = 24/50 (48%)
Frame = +1
Query: 475 QGSGGVPARKS*QDYKNKIKKDVVLKVDTENFLSPDTCGGIELVAARGRI 624
QG+ G + + N + +D+ L++ TE L GG E V GRI
Sbjct: 280 QGAAGGAEARPFVTFHNSLGRDLYLRIATELHLKRMLVGGFEKVYEIGRI 329
>At3g10040.1 68416.m01204 expressed protein est match
Length = 431
Score = 27.9 bits (59), Expect = 8.6
Identities = 12/32 (37%), Positives = 21/32 (65%)
Frame = +3
Query: 180 KGRLVQQQRLKIMEYYEKKEKQVELRRRSNLR 275
+G +++QR+K M Y KKE+++E + N R
Sbjct: 364 EGVEMEKQRVKWMRYRSKKEREMEKAKLDNQR 395
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,506,355
Number of Sequences: 28952
Number of extensions: 305135
Number of successful extensions: 883
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 854
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 881
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1882599200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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