Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ps4M0191.Seq
         (953 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    31   0.18 
SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc...    26   9.0  

>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 31.5 bits (68), Expect = 0.18
 Identities = 20/65 (30%), Positives = 33/65 (50%), Gaps = 5/65 (7%)
 Frame = +1

Query: 493 PVPNSPIVSRITIHWPSFYNVVTGKTLALPNLIALQ-----HIPLSPAGVIAKRPAPIAL 657
           P+P  P+ S ++ H   +    +    +L N I+L      ++PLSP    A+ P+PI L
Sbjct: 170 PLPRPPLPSSVSSHSSPYSTTSSTSLYSLYNDISLSCSPEPYLPLSPTRSPARTPSPIRL 229

Query: 658 PNSCA 672
            +S A
Sbjct: 230 YSSDA 234


>SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 732

 Score = 25.8 bits (54), Expect = 9.0
 Identities = 12/25 (48%), Positives = 14/25 (56%)
 Frame = +3

Query: 606 PPFASWRNSEEARTDRPSQQLRSXN 680
           PP AS RN  E  +  PS+Q  S N
Sbjct: 353 PPGASGRNRRERTSSTPSEQSTSVN 377


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,718,039
Number of Sequences: 5004
Number of extensions: 73886
Number of successful extensions: 128
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 126
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 128
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 487313384
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)