BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ps4M0165.Seq
(853 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3; Eukaryo... 65 2e-09
UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1; ... 64 3e-09
UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular organ... 64 3e-09
UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: L... 64 3e-09
UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep: Bet... 64 3e-09
UniRef50_A0ZLG1 Cluster: Beta-D-galactosidase; n=1; Nodularia sp... 57 6e-07
UniRef50_A6FJQ2 Cluster: 50S ribosomal protein L5; n=8; Bacteria... 50 7e-05
UniRef50_Q669R9 Cluster: Beta-galactosidase; n=14; Yersinia|Rep:... 46 0.001
UniRef50_A7MN76 Cluster: Putative uncharacterized protein; n=1; ... 40 0.079
UniRef50_P06219 Cluster: Beta-galactosidase; n=11; Gammaproteoba... 39 0.18
UniRef50_Q4Z0C1 Cluster: Putative uncharacterized protein; n=3; ... 37 0.56
UniRef50_Q48727 Cluster: Beta-galactosidase; n=3; Lactococcus la... 36 1.3
UniRef50_P81650 Cluster: Beta-galactosidase; n=26; Gammaproteoba... 35 2.3
UniRef50_Q5DC94 Cluster: SJCHGC09076 protein; n=1; Schistosoma j... 34 4.0
UniRef50_Q2TFW2 Cluster: Leucine-rich-repeat protein 10; n=2; Pl... 34 4.0
UniRef50_A2VBJ9 Cluster: Non-ribosomal peptide synthetase; n=1; ... 34 5.2
UniRef50_UPI0000498CC4 Cluster: predicted protein; n=3; Entamoeb... 33 6.9
>UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3;
Eukaryota|Rep: beta-galactosidase - Entamoeba
histolytica HM-1:IMSS
Length = 86
Score = 64.9 bits (151), Expect = 2e-09
Identities = 28/28 (100%), Positives = 28/28 (100%)
Frame = +1
Query: 424 HWPSFYNVVTGKTLALPNLIALQHIPLS 507
HWPSFYNVVTGKTLALPNLIALQHIPLS
Sbjct: 5 HWPSFYNVVTGKTLALPNLIALQHIPLS 32
Score = 35.5 bits (78), Expect = 1.7
Identities = 17/22 (77%), Positives = 20/22 (90%)
Frame = +2
Query: 521 SEEARTDRPSQQLRSLNGEWQI 586
SEEARTDRPSQQLRSL +W++
Sbjct: 38 SEEARTDRPSQQLRSL--KWRM 57
>UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1;
Erwinia amylovora|Rep: Putative uncharacterized protein
- Erwinia amylovora (Fire blight bacteria)
Length = 123
Score = 64.5 bits (150), Expect = 3e-09
Identities = 28/29 (96%), Positives = 29/29 (100%)
Frame = +3
Query: 426 LAVVLQRRDWENPGVTQLNRLAAHPPFAA 512
LAVVLQRRDWENPGVTQLNRLAAHPPFA+
Sbjct: 68 LAVVLQRRDWENPGVTQLNRLAAHPPFAS 96
Score = 60.1 bits (139), Expect = 7e-08
Identities = 25/28 (89%), Positives = 26/28 (92%)
Frame = +2
Query: 500 PFRSWRNSEEARTDRPSQQLRSLNGEWQ 583
PF SWRNSEEARTDRPSQQLR LNGEW+
Sbjct: 93 PFASWRNSEEARTDRPSQQLRXLNGEWR 120
>UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular
organisms|Rep: LacZ-alpha peptide - Escherichia coli
Length = 90
Score = 64.5 bits (150), Expect = 3e-09
Identities = 28/29 (96%), Positives = 29/29 (100%)
Frame = +3
Query: 426 LAVVLQRRDWENPGVTQLNRLAAHPPFAA 512
LAVVLQRRDWENPGVTQLNRLAAHPPFA+
Sbjct: 22 LAVVLQRRDWENPGVTQLNRLAAHPPFAS 50
Score = 50.4 bits (115), Expect = 6e-05
Identities = 22/23 (95%), Positives = 22/23 (95%)
Frame = +2
Query: 500 PFRSWRNSEEARTDRPSQQLRSL 568
PF SWRNSEEARTDRPSQQLRSL
Sbjct: 47 PFASWRNSEEARTDRPSQQLRSL 69
>UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: LacZ
protein - Phage M13mp18
Length = 102
Score = 64.5 bits (150), Expect = 3e-09
Identities = 28/29 (96%), Positives = 29/29 (100%)
Frame = +3
Query: 426 LAVVLQRRDWENPGVTQLNRLAAHPPFAA 512
LAVVLQRRDWENPGVTQLNRLAAHPPFA+
Sbjct: 26 LAVVLQRRDWENPGVTQLNRLAAHPPFAS 54
Score = 62.1 bits (144), Expect = 2e-08
Identities = 26/28 (92%), Positives = 27/28 (96%)
Frame = +2
Query: 500 PFRSWRNSEEARTDRPSQQLRSLNGEWQ 583
PF SWRNSEEARTDRPSQQLRSLNGEW+
Sbjct: 51 PFASWRNSEEARTDRPSQQLRSLNGEWR 78
>UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep:
Beta-galactosidase - Escherichia coli (strain K12)
Length = 1024
Score = 64.5 bits (150), Expect = 3e-09
Identities = 28/29 (96%), Positives = 29/29 (100%)
Frame = +3
Query: 426 LAVVLQRRDWENPGVTQLNRLAAHPPFAA 512
LAVVLQRRDWENPGVTQLNRLAAHPPFA+
Sbjct: 8 LAVVLQRRDWENPGVTQLNRLAAHPPFAS 36
Score = 62.1 bits (144), Expect = 2e-08
Identities = 26/28 (92%), Positives = 27/28 (96%)
Frame = +2
Query: 500 PFRSWRNSEEARTDRPSQQLRSLNGEWQ 583
PF SWRNSEEARTDRPSQQLRSLNGEW+
Sbjct: 33 PFASWRNSEEARTDRPSQQLRSLNGEWR 60
>UniRef50_A0ZLG1 Cluster: Beta-D-galactosidase; n=1; Nodularia
spumigena CCY 9414|Rep: Beta-D-galactosidase - Nodularia
spumigena CCY 9414
Length = 72
Score = 56.8 bits (131), Expect = 6e-07
Identities = 23/26 (88%), Positives = 26/26 (100%)
Frame = +2
Query: 512 WRNSEEARTDRPSQQLRSLNGEWQIV 589
WRNSEEARTDRPSQQLRSLNGEW+++
Sbjct: 47 WRNSEEARTDRPSQQLRSLNGEWRLM 72
>UniRef50_A6FJQ2 Cluster: 50S ribosomal protein L5; n=8;
Bacteria|Rep: 50S ribosomal protein L5 - Moritella sp.
PE36
Length = 45
Score = 50.0 bits (114), Expect = 7e-05
Identities = 25/36 (69%), Positives = 27/36 (75%)
Frame = -1
Query: 589 YNLPFAIQAAQLLGRAIGAGLFAITPAAKGGCAARR 482
+ PFAIQAAQLLGRAIGAGLFAITP + G R
Sbjct: 8 HQAPFAIQAAQLLGRAIGAGLFAITPEFELGTPESR 43
>UniRef50_Q669R9 Cluster: Beta-galactosidase; n=14; Yersinia|Rep:
Beta-galactosidase - Yersinia pseudotuberculosis
Length = 1066
Score = 46.0 bits (104), Expect = 0.001
Identities = 18/27 (66%), Positives = 21/27 (77%)
Frame = +3
Query: 426 LAVVLQRRDWENPGVTQLNRLAAHPPF 506
L +L RRDWENP +TQ +RL AHPPF
Sbjct: 15 LPQILSRRDWENPQITQYHRLEAHPPF 41
Score = 40.7 bits (91), Expect = 0.046
Identities = 16/27 (59%), Positives = 20/27 (74%)
Frame = +2
Query: 500 PFRSWRNSEEARTDRPSQQLRSLNGEW 580
PF SWR+ E A+ DRPS Q ++LNG W
Sbjct: 40 PFHSWRDVESAQKDRPSPQQQTLNGLW 66
>UniRef50_A7MN76 Cluster: Putative uncharacterized protein; n=1;
Enterobacter sakazakii ATCC BAA-894|Rep: Putative
uncharacterized protein - Enterobacter sakazakii ATCC
BAA-894
Length = 1043
Score = 39.9 bits (89), Expect = 0.079
Identities = 14/26 (53%), Positives = 19/26 (73%)
Frame = +3
Query: 426 LAVVLQRRDWENPGVTQLNRLAAHPP 503
LA +L R DW+NP +T +NRL +H P
Sbjct: 18 LATILARNDWQNPAITSVNRLPSHTP 43
Score = 37.1 bits (82), Expect = 0.56
Identities = 14/31 (45%), Positives = 20/31 (64%)
Frame = +2
Query: 491 STSPFRSWRNSEEARTDRPSQQLRSLNGEWQ 583
S +P WR+++ AR PS + SL+GEWQ
Sbjct: 40 SHTPLHGWRDADRARRGEPSDAVLSLDGEWQ 70
>UniRef50_P06219 Cluster: Beta-galactosidase; n=11;
Gammaproteobacteria|Rep: Beta-galactosidase - Klebsiella
pneumoniae
Length = 1034
Score = 38.7 bits (86), Expect = 0.18
Identities = 16/26 (61%), Positives = 18/26 (69%)
Frame = +3
Query: 435 VLQRRDWENPGVTQLNRLAAHPPFAA 512
VL R DW N +T LNRL AHP FA+
Sbjct: 17 VLAREDWHNQTITHLNRLPAHPVFAS 42
>UniRef50_Q4Z0C1 Cluster: Putative uncharacterized protein; n=3;
Plasmodium (Vinckeia)|Rep: Putative uncharacterized
protein - Plasmodium berghei
Length = 275
Score = 37.1 bits (82), Expect = 0.56
Identities = 16/16 (100%), Positives = 16/16 (100%)
Frame = +1
Query: 373 RGGARYPIRPIVSRIT 420
RGGARYPIRPIVSRIT
Sbjct: 260 RGGARYPIRPIVSRIT 275
>UniRef50_Q48727 Cluster: Beta-galactosidase; n=3; Lactococcus
lactis|Rep: Beta-galactosidase - Lactococcus lactis
subsp. lactis (Streptococcus lactis)
Length = 998
Score = 35.9 bits (79), Expect = 1.3
Identities = 14/23 (60%), Positives = 17/23 (73%)
Frame = +3
Query: 435 VLQRRDWENPGVTQLNRLAAHPP 503
VL+R+DWENP V+ NRL H P
Sbjct: 9 VLERKDWENPVVSNWNRLPMHTP 31
>UniRef50_P81650 Cluster: Beta-galactosidase; n=26;
Gammaproteobacteria|Rep: Beta-galactosidase -
Pseudoalteromonas haloplanktis (Alteromonas
haloplanktis)
Length = 1039
Score = 35.1 bits (77), Expect = 2.3
Identities = 12/23 (52%), Positives = 17/23 (73%)
Frame = +3
Query: 435 VLQRRDWENPGVTQLNRLAAHPP 503
++ RRDWENP Q+N++ AH P
Sbjct: 7 IINRRDWENPITVQVNQVKAHSP 29
>UniRef50_Q5DC94 Cluster: SJCHGC09076 protein; n=1; Schistosoma
japonicum|Rep: SJCHGC09076 protein - Schistosoma
japonicum (Blood fluke)
Length = 109
Score = 34.3 bits (75), Expect = 4.0
Identities = 17/37 (45%), Positives = 21/37 (56%)
Frame = +3
Query: 429 AVVLQRRDWENPGVTQLNRLAAHPPFAAGVIAKRPAP 539
A L+RR+ +NPG QLN L A P F G K+ P
Sbjct: 57 AAFLKRREGKNPGCPQLNPLEALPLFPGGEKTKKAPP 93
>UniRef50_Q2TFW2 Cluster: Leucine-rich-repeat protein 10; n=2;
Plasmodium falciparum|Rep: Leucine-rich-repeat protein
10 - Plasmodium falciparum
Length = 317
Score = 34.3 bits (75), Expect = 4.0
Identities = 26/108 (24%), Positives = 52/108 (48%), Gaps = 2/108 (1%)
Frame = +3
Query: 6 DNPRREMSFE-RSPWCDFVGVNINDVYNNITIT*RRRKLN*I*FTSVHTVH*KCI-IIHN 179
DN +++ F ++ CD + Y +T R++ ++ I + +H K I + HN
Sbjct: 55 DNKEKDIMFNIKNGLCDLEKTLDGEGYAYSNLTCRKKGIDFIPKSITRYIHLKYINLSHN 114
Query: 180 KLLIKFHIIYLNRIIFKHLLCEI*SIVVLIKKTKFISRSYLNVSYALL 323
+ H+ +L IIF + + +V +KK + Y+N+S+ L+
Sbjct: 115 NINDLVHLYFLPNIIFLDVSYNMLKEIVELKKEYLKNCIYINLSHNLI 162
>UniRef50_A2VBJ9 Cluster: Non-ribosomal peptide synthetase; n=1;
uncultured bacterium|Rep: Non-ribosomal peptide
synthetase - uncultured bacterium
Length = 338
Score = 33.9 bits (74), Expect = 5.2
Identities = 18/35 (51%), Positives = 18/35 (51%)
Frame = -1
Query: 475 WVTPGFSQSRRCKTTASEL*YDSL*GELGTGPPLE 371
W GF C YDSL GELGTGPPLE
Sbjct: 260 WSKTGFRPF--CLEAGRRAYYDSLYGELGTGPPLE 292
>UniRef50_UPI0000498CC4 Cluster: predicted protein; n=3; Entamoeba
histolytica HM-1:IMSS|Rep: predicted protein - Entamoeba
histolytica HM-1:IMSS
Length = 349
Score = 33.5 bits (73), Expect = 6.9
Identities = 23/68 (33%), Positives = 32/68 (47%), Gaps = 2/68 (2%)
Frame = -2
Query: 783 SGFSPXDLESTFFNRDS-CSKLEQHSTLSRSILLIYKGFXPISAYWLK-NELI*QKFNAN 610
S + L+ T FN D C K ++ ++ LLIY G+ P WLK E+I + N
Sbjct: 50 SNYKDKLLDLTHFNYDGECGKPFKYKPNNKRDLLIYGGYFPDKQQWLKQREVIMNTLSLN 109
Query: 609 FNKILTLT 586
N I T
Sbjct: 110 QNGIPNAT 117
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 784,314,784
Number of Sequences: 1657284
Number of extensions: 14832724
Number of successful extensions: 29892
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 29133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 29890
length of database: 575,637,011
effective HSP length: 100
effective length of database: 409,908,611
effective search space used: 75013275813
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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