Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ps4M0155.Seq
         (948 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g04350.1 68417.m00622 leucyl-tRNA synthetase, putative / leuc...    35   0.091
At5g51300.2 68418.m06360 splicing factor-related contains simila...    31   1.5  
At5g51300.1 68418.m06359 splicing factor-related contains simila...    31   1.5  
At5g49030.1 68418.m06067 tRNA synthetase class I (I, L, M and V)...    29   4.5  
At5g17970.1 68418.m02108 disease resistance protein (TIR-NBS-LRR...    29   4.5  
At4g32370.1 68417.m04609 glycoside hydrolase family 28 protein /...    29   4.5  
At1g27160.1 68414.m03309 valyl-tRNA synthetase / valine--tRNA li...    28   7.9  

>At4g04350.1 68417.m00622 leucyl-tRNA synthetase, putative /
            leucine--tRNA ligase, putative similar to SP|P36430
            Leucyl-tRNA synthetase (EC 6.1.1.4) (Leucine--tRNA
            ligase) (LeuRS) {Bacillus subtilis}; contains Pfam
            profile PF00133: tRNA synthetases class I (I, L, M and V)
          Length = 973

 Score = 34.7 bits (76), Expect = 0.091
 Identities = 15/33 (45%), Positives = 22/33 (66%)
 Frame = +2

Query: 620  TEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGK 718
            +E+     A Q+  + KYLDG +++K IYVPGK
Sbjct: 925  SEDDAFVLASQDDKLRKYLDGQSIKKRIYVPGK 957



 Score = 33.1 bits (72), Expect = 0.28
 Identities = 34/115 (29%), Positives = 51/115 (44%), Gaps = 1/115 (0%)
 Frame = +3

Query: 267  RDVHKTIAKVTDDIGRRQTFNTAIAGDYGADEQTGESTNRWRAGSRSDAGS-TAGRCPYA 443
            R +HK IAKVT++I   + FNT I+G      +   +  +W    R           PYA
Sbjct: 815  RTLHKCIAKVTEEIESTR-FNTGISGMM----EFVNAAYKWNNQPRGIIEPFVLLLSPYA 869

Query: 444  *PVHPAHLLHAVAGTERRRRYRNAPWPVADEKAMVEDSTLVVVQVNGKVRAKITV 608
               H A  L +  G      Y +  +P A+   +   + ++ VQ+NGK R  I V
Sbjct: 870  --PHMAEELWSRLGHPNSLAYES--FPKANPDYLKNTTIVLPVQINGKTRGTIEV 920


>At5g51300.2 68418.m06360 splicing factor-related contains
           similarity to SF1 protein [Drosophila melanogaster]
           GI:6687400
          Length = 804

 Score = 30.7 bits (66), Expect = 1.5
 Identities = 16/56 (28%), Positives = 25/56 (44%)
 Frame = -1

Query: 216 MPDNP*YAPSPPTRDRYYGCHARC*YKWGIAYRAPPERFLRIHQCQTTKSPAGSAP 49
           +P    YA  PP    Y+  H +    +G+ Y  PP    +     TT++P+ S P
Sbjct: 609 VPSYSPYALPPPPPGSYHPVHGQHMPPYGMQYPPPPPHVTQAPPPGTTQNPSSSEP 664


>At5g51300.1 68418.m06359 splicing factor-related contains
           similarity to SF1 protein [Drosophila melanogaster]
           GI:6687400
          Length = 804

 Score = 30.7 bits (66), Expect = 1.5
 Identities = 16/56 (28%), Positives = 25/56 (44%)
 Frame = -1

Query: 216 MPDNP*YAPSPPTRDRYYGCHARC*YKWGIAYRAPPERFLRIHQCQTTKSPAGSAP 49
           +P    YA  PP    Y+  H +    +G+ Y  PP    +     TT++P+ S P
Sbjct: 609 VPSYSPYALPPPPPGSYHPVHGQHMPPYGMQYPPPPPHVTQAPPPGTTQNPSSSEP 664


>At5g49030.1 68418.m06067 tRNA synthetase class I (I, L, M and V)
           family protein similar to SP|P41972 Isoleucyl-tRNA
           synthetase (EC 6.1.1.5) (Isoleucine--tRNA ligase)
           (IleRS) {Staphylococcus aureus}; contains Pfam profile
           PF00133: tRNA synthetases class I (I, L, M and V)
          Length = 1093

 Score = 29.1 bits (62), Expect = 4.5
 Identities = 10/33 (30%), Positives = 20/33 (60%)
 Frame = +1

Query: 400 RALMQEALLAVVRMLNPFTPHICFTLWQELKGE 498
           + ++   LL+++R++ P  PH+   +WQ L  E
Sbjct: 878 QTVLSTHLLSILRVIAPIVPHLAEDVWQNLPFE 910


>At5g17970.1 68418.m02108 disease resistance protein (TIR-NBS-LRR
           class), putative domain signature TIR-NBS-LRR exists,
           suggestive of a disease resistance protein.
          Length = 780

 Score = 29.1 bits (62), Expect = 4.5
 Identities = 20/61 (32%), Positives = 30/61 (49%), Gaps = 4/61 (6%)
 Frame = +3

Query: 264 RRDVHKTIAKVTDDIG----RRQTFNTAIAGDYGADEQTGESTNRWRAGSRSDAGSTAGR 431
           R  V KT+  +  D+     R+QT +   A D   D +T E   RWR  + ++ G+ AG 
Sbjct: 97  REVVGKTLVPIFYDVDPSSVRKQTGDFGKAFDKICDVRTEEERQRWRQ-ALTNVGNIAGE 155

Query: 432 C 434
           C
Sbjct: 156 C 156


>At4g32370.1 68417.m04609 glycoside hydrolase family 28 protein /
           polygalacturonase (pectinase) family protein similar to
           polygalacturonase [Lycopersicon esculentum] GI:4325090;
           contains PF00295: Glycosyl hydrolases family 28
           (polygalacturonases)
          Length = 342

 Score = 29.1 bits (62), Expect = 4.5
 Identities = 16/57 (28%), Positives = 24/57 (42%)
 Frame = +1

Query: 76  GLALMNPQKSFWWRSVRYSPFISTPGMASVISIAGWWRWRVLWIIRHTAVYPCDALT 246
           G  ++NP    WW+SV  S   +T   AS + I       +     H ++Y C   T
Sbjct: 134 GSGVLNPHGEAWWKSVSLSKRPTTISFASCMDIVYNGLHHINSPRNHISIYGCTNAT 190


>At1g27160.1 68414.m03309 valyl-tRNA synthetase / valine--tRNA
           ligase-related similar to valyl tRNA synthetase
           GI:1890130 from [Arabidopsis thaliana]
          Length = 200

 Score = 28.3 bits (60), Expect = 7.9
 Identities = 11/24 (45%), Positives = 15/24 (62%)
 Frame = +1

Query: 433 VRMLNPFTPHICFTLWQELKGEGD 504
           +R+L+PF P+I   LWQ L    D
Sbjct: 53  LRLLHPFMPYITEELWQRLPSSQD 76


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,185,320
Number of Sequences: 28952
Number of extensions: 465734
Number of successful extensions: 1216
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 1164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1216
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2275754832
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)