BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ps4M0065.Seq (620 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC63.12c |||20S proteasome component beta 3|Schizosaccharomyce... 83 2e-17 SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar... 35 0.011 SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces po... 27 2.9 SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizos... 25 6.7 SPAC343.12 |rds1||conserved fungal protein|Schizosaccharomyces p... 25 8.8 >SPCC63.12c |||20S proteasome component beta 3|Schizosaccharomyces pombe|chr 3|||Manual Length = 204 Score = 83.4 bits (197), Expect = 2e-17 Identities = 39/103 (37%), Positives = 62/103 (60%) Frame = +3 Query: 78 MSILAYNGGAVVAMKGQDCVAIATDKRFGIQAQTVSTQLPKSIPXGTHXVXRPSRXRX*T 257 MSI+ YNGG+ VAM G++CVAIA+D R G+Q+ +++ PK G + Sbjct: 1 MSIMEYNGGSCVAMAGKNCVAIASDLRLGVQSISLTNNFPKVFAMGDKTYLGLTGLATDV 60 Query: 258 QTVXQXXXFRXNLYXLKEXXLXRLKXFSXMLSTLLYDRRFGPY 386 QT+ + ++ NLY +E + K F+ ++S+ LY++RFGPY Sbjct: 61 QTLYELFRYKVNLYKFREERQIQPKTFANLVSSTLYEKRFGPY 103 Score = 40.7 bits (91), Expect = 2e-04 Identities = 23/65 (35%), Positives = 34/65 (52%) Frame = +2 Query: 374 FRPLLMEPVIAGLDPLX*PNPYGLXHGLIGCPNDPEDFVGIGPLFEKLYGMCXALWGXPT 553 F P PV+AG+ P+ IGC + EDF+ G E+LYGMC +++ P Sbjct: 100 FGPYFSFPVVAGVSNDN--TPFICGFDSIGCIDFAEDFIVSGTATEQLYGMCESVY-EPN 156 Query: 554 XKPDE 568 +PD+ Sbjct: 157 LEPDD 161 >SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosaccharomyces pombe|chr 1|||Manual Length = 225 Score = 34.7 bits (76), Expect = 0.011 Identities = 26/98 (26%), Positives = 40/98 (40%), Gaps = 1/98 (1%) Frame = +3 Query: 96 NGGAVVAMKGQDCVAIATDKRFGIQAQTVSTQL-PKSIPXGTHXVXRPSRXRX*TQTVXQ 272 NGG VA+ G +A D R + ++T+ P+ G V S + + Sbjct: 12 NGGTTVAIAGDGFAILAGDTR-SVNGYNINTRFQPRVHEVGDDLVIGASGFEADALALVK 70 Query: 273 XXXFRXNLYXLKEXXLXRLKXFSXMLSTLLYDRRFGPY 386 R +LY + + M+ TLLY +RF PY Sbjct: 71 RIQQRIDLYHDNHERKMSAQSCACMVRTLLYGKRFFPY 108 >SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 981 Score = 26.6 bits (56), Expect = 2.9 Identities = 15/43 (34%), Positives = 24/43 (55%), Gaps = 4/43 (9%) Frame = +3 Query: 6 IQYFELITLQ*FQVKKCLSFYITNMSIL----AYNGGAVVAMK 122 ++YF+ + + +VK SF+I N +L AYNG V +K Sbjct: 441 LKYFDQVVVGLKEVKDAFSFFIFNRFVLVYVPAYNGVDPVTLK 483 >SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 400 Score = 25.4 bits (53), Expect = 6.7 Identities = 12/45 (26%), Positives = 17/45 (37%) Frame = -3 Query: 579 SPRXSSGFXVGXPQXAXHIPYSFSNRGPIPTKSSGSFGQPIKPXY 445 +P+ F Q A P S R P+P K ++P Y Sbjct: 342 NPKTPQSFSTNIVQCASPSPLPLSFRSPVPNKDYEYIPSSLQPQY 386 >SPAC343.12 |rds1||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 402 Score = 25.0 bits (52), Expect = 8.8 Identities = 10/19 (52%), Positives = 12/19 (63%) Frame = +2 Query: 443 LXHGLIGCPNDPEDFVGIG 499 L G+IG DP +F GIG Sbjct: 15 LARGIIGAKADPVNFAGIG 33 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,318,503 Number of Sequences: 5004 Number of extensions: 42480 Number of successful extensions: 80 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 76 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 78 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 273658928 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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