Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ps4M0056.Seq
(875 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P43753 Cluster: Formate acetyltransferase; n=271; cellu... 157 3e-37
UniRef50_A7MET3 Cluster: Putative uncharacterized protein; n=1; ... 136 5e-31
UniRef50_Q8G5Q3 Cluster: Formate acetyltransferase; n=5; Actinob... 118 2e-25
UniRef50_Q8FJC0 Cluster: Putative uncharacterized protein; n=1; ... 103 6e-21
UniRef50_Q59934 Cluster: Formate acetyltransferase; n=55; Bacill... 101 2e-20
UniRef50_A4E923 Cluster: Putative uncharacterized protein; n=1; ... 69 1e-10
UniRef50_Q7NEY2 Cluster: Glr3745 protein; n=1; Gloeobacter viola... 65 2e-09
UniRef50_Q18AX8 Cluster: Glycerol dehydratase; n=5; Clostridiace... 41 0.047
UniRef50_O06962 Cluster: Hypothetical pyruvate formate-lyase; n=... 41 0.047
UniRef50_A6TKL5 Cluster: Pyruvate formate-lyase; n=1; Alkaliphil... 37 0.58
UniRef50_Q896R2 Cluster: Formate acetyltransferase 2; n=5; Bacte... 36 1.4
UniRef50_Q4RTJ3 Cluster: Chromosome 2 SCAF14997, whole genome sh... 34 5.5
UniRef50_Q0CS91 Cluster: Protein TOXD; n=2; Aspergillus|Rep: Pro... 34 5.5
>UniRef50_P43753 Cluster: Formate acetyltransferase; n=271; cellular
organisms|Rep: Formate acetyltransferase - Haemophilus
influenzae
Length = 770
Score = 157 bits (381), Expect = 3e-37
Identities = 71/84 (84%), Positives = 78/84 (92%)
Frame = -3
Query: 255 SLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGGVDEKLK 76
S+QYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKT+LYAINGG+DEKL
Sbjct: 397 SVQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTLLYAINGGIDEKLG 456
Query: 75 MQVGPKSEPIKGDVLNYDEVMERM 4
MQVGPK+ PI +VL++D VM RM
Sbjct: 457 MQVGPKTAPITDEVLDFDTVMTRM 480
Score = 156 bits (379), Expect = 6e-37
Identities = 70/85 (82%), Positives = 79/85 (92%)
Frame = -1
Query: 509 ELRMVRFLRTPEYDELFSGDPIWATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPN 330
+LRMVRFLRTPEYD+LFSGDP+WATE+I GMGLDGRTLVTKN+FR L+TLY MG SPEPN
Sbjct: 312 KLRMVRFLRTPEYDQLFSGDPMWATETIAGMGLDGRTLVTKNTFRILHTLYNMGTSPEPN 371
Query: 329 MTILWSEKLPLNFKKFAAKVSIDTS 255
+TILWSE+LP NFK+F AKVSIDTS
Sbjct: 372 LTILWSEQLPENFKRFCAKVSIDTS 396
Score = 88.6 bits (210), Expect = 2e-16
Identities = 42/54 (77%), Positives = 46/54 (85%)
Frame = -2
Query: 670 WT*FGYLAAVKSQNXAAMSSXRTSTFLDVYIERDLKAXKITEQEAQEMVDHLVM 509
W F YLAA+KSQN AAMS RT+TF+DVYIERDLKA KITE EAQE+VDHLVM
Sbjct: 258 WMYFAYLAAIKSQNGAAMSFGRTATFIDVYIERDLKAGKITETEAQELVDHLVM 311
Score = 46.8 bits (106), Expect = 7e-04
Identities = 24/36 (66%), Positives = 26/36 (72%)
Frame = -3
Query: 780 KEIA*THRGSGFRLKXMAAKYGYDISGPATNGSEAI 673
+EIA HR G +LK MAA YGYDIS PATN EAI
Sbjct: 222 EEIAEQHRALG-QLKQMAASYGYDISNPATNAQEAI 256
>UniRef50_A7MET3 Cluster: Putative uncharacterized protein; n=1;
Enterobacter sakazakii ATCC BAA-894|Rep: Putative
uncharacterized protein - Enterobacter sakazakii ATCC
BAA-894
Length = 783
Score = 136 bits (330), Expect = 5e-31
Identities = 62/84 (73%), Positives = 70/84 (83%)
Frame = +1
Query: 4 HALHHFIIVQDIAFDRFRLRTNLHFQFFVNAAVDCVQHGFRQVCTRTEELHLFTNDHRAY 183
H H+F+ +Q IAFDRFR R LHFQFF+NAAVD VQHGFR+V TEELHL TN HRAY
Sbjct: 312 HTFHNFVEIQQIAFDRFRFRAYLHFQFFINAAVDSVQHGFREVSASTEELHLLTNHHRAY 371
Query: 184 AASNSVVIVVEVRTHQVIVLILQR 255
AA +SVV+VVEVRTHQVIVL+LQR
Sbjct: 372 AACDSVVVVVEVRTHQVIVLVLQR 395
Score = 130 bits (314), Expect = 5e-29
Identities = 62/85 (72%), Positives = 67/85 (78%)
Frame = +3
Query: 255 RGVDGHFSGEFLEVQRQFFRPQNGHVRFRRRTHGVQGVQETEAVFGNQGTTVEAHTTDRF 434
RGVDG+F EF EVQRQFF PQN VR RRR HGV+GVQE EAVFG Q T V AHTT+ F
Sbjct: 396 RGVDGNFRREFFEVQRQFFGPQNRDVRLRRRPHGVEGVQEAEAVFGYQRTAVNAHTTNGF 455
Query: 435 GCPDRVAREQFIVFRSTQEANHTQF 509
G PDRVA EQ I+FR TQEA+HTQF
Sbjct: 456 GRPDRVAGEQLIIFRCTQEAHHTQF 480
Score = 64.9 bits (151), Expect = 3e-09
Identities = 33/75 (44%), Positives = 44/75 (58%)
Frame = +2
Query: 446 SGRQRTVHRIPEYAGSEPYAVHDQVVNHFLRFLFGDLXSFQVTFDVHIQEGGSTXRRHCS 625
+G Q + R + A + H QVV+ FL FL G+ FQV FDVH++EGG + H
Sbjct: 462 AGEQLIIFRCTQEAHHTQF--HHQVVDQFLGFLLGEFACFQVAFDVHVKEGGDAAKGHRG 519
Query: 626 XVLRLNSSQVAELSP 670
VL + SQVAE+SP
Sbjct: 520 TVLGFHRSQVAEVSP 534
>UniRef50_Q8G5Q3 Cluster: Formate acetyltransferase; n=5;
Actinobacteridae|Rep: Formate acetyltransferase -
Bifidobacterium longum
Length = 804
Score = 118 bits (284), Expect = 2e-25
Identities = 55/87 (63%), Positives = 66/87 (75%), Gaps = 2/87 (2%)
Frame = -1
Query: 509 ELRMVRFLRTPEYDELFSGDPIWATESIGGMGLDGRTLVTKNSFRFLNTLYT--MGPSPE 336
+LR+VRFLRT +YD +FSGDP WAT S G G DGR++VTK SFR LNTL +GP PE
Sbjct: 335 KLRIVRFLRTKDYDSIFSGDPYWATWSDAGFGDDGRSMVTKTSFRLLNTLTLEHLGPGPE 394
Query: 335 PNMTILWSEKLPLNFKKFAAKVSIDTS 255
PN+TI W KLP +K+F AK+SIDTS
Sbjct: 395 PNITIFWDPKLPEAYKRFCAKISIDTS 421
Score = 85.0 bits (201), Expect = 2e-15
Identities = 48/85 (56%), Positives = 58/85 (68%), Gaps = 5/85 (5%)
Frame = -3
Query: 255 SLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGGVDEKLK 76
++QYE+D +R + DD AIACCVSPM VGKQMQFF AR N AK +LYAINGG DE
Sbjct: 422 AIQYESDKEIRSHWG-DDAAIACCVSPMRVGKQMQFFAARVNSAKALLYAINGGRDEMTG 480
Query: 75 MQVGPKS--EPIKGD---VLNYDEV 16
MQV K EPI + L+Y++V
Sbjct: 481 MQVIDKGVIEPITPEADGTLDYEKV 505
Score = 77.0 bits (181), Expect = 6e-13
Identities = 35/54 (64%), Positives = 44/54 (81%)
Frame = -2
Query: 670 WT*FGYLAAVKSQNXAAMSSXRTSTFLDVYIERDLKAXKITEQEAQEMVDHLVM 509
WT GYLA++KSQ+ AAMS R S FLD YIERDL+A KITE +AQE++D++VM
Sbjct: 281 WTYMGYLASIKSQDGAAMSFGRNSAFLDCYIERDLQAGKITETDAQELIDNIVM 334
>UniRef50_Q8FJC0 Cluster: Putative uncharacterized protein; n=1;
Escherichia coli O6|Rep: Putative uncharacterized
protein - Escherichia coli O6
Length = 404
Score = 103 bits (247), Expect = 6e-21
Identities = 53/79 (67%), Positives = 59/79 (74%)
Frame = +3
Query: 273 FSGEFLEVQRQFFRPQNGHVRFRRRTHGVQGVQETEAVFGNQGTTVEAHTTDRFGCPDRV 452
FSG F + + F +G + +R +ETEAVFGNQGTTVEAHTTDRFGCPDRV
Sbjct: 30 FSGSFSDHRMVMFGSGDGPMVYRV-------FRETEAVFGNQGTTVEAHTTDRFGCPDRV 82
Query: 453 AREQFIVFRSTQEANHTQF 509
AREQFIVFRSTQEANHTQF
Sbjct: 83 AREQFIVFRSTQEANHTQF 101
Score = 102 bits (244), Expect = 1e-20
Identities = 47/54 (87%), Positives = 48/54 (88%)
Frame = +2
Query: 509 HDQVVNHFLRFLFGDLXSFQVTFDVHIQEGGSTXRRHCSXVLRLNSSQVAELSP 670
HDQVVNHFLRFLFGDL SFQVTFDVHIQEGG T HCS VLRLNSSQVAE+SP
Sbjct: 102 HDQVVNHFLRFLFGDLASFQVTFDVHIQEGGGTTEGHCSTVLRLNSSQVAEVSP 155
Score = 86.2 bits (204), Expect = 1e-15
Identities = 39/42 (92%), Positives = 41/42 (97%)
Frame = +1
Query: 250 QREVSMDTLAANFLKFSGSFSDHRMVMFGSGDGPMVYRVFRK 375
+ EVSMDTLAANFLKFSGSFSDHRMVMFGSGDGPMVYRVFR+
Sbjct: 15 REEVSMDTLAANFLKFSGSFSDHRMVMFGSGDGPMVYRVFRE 56
Score = 44.4 bits (100), Expect = 0.004
Identities = 23/42 (54%), Positives = 28/42 (66%)
Frame = +1
Query: 655 SRIKSMDSF*TVGSXTRDVVAVFXSHXFQSEPRXAVCLSDFF 780
+ + +DSF +VGS TRDVVAVF SH F + AV SDFF
Sbjct: 151 AEVSPLDSFLSVGSRTRDVVAVFRSHFFHL-TQSAVLFSDFF 191
Score = 35.9 bits (79), Expect = 1.4
Identities = 15/16 (93%), Positives = 16/16 (100%)
Frame = +2
Query: 209 LLKSGRIRSSFSYCRE 256
+LKSGRIRSSFSYCRE
Sbjct: 1 MLKSGRIRSSFSYCRE 16
>UniRef50_Q59934 Cluster: Formate acetyltransferase; n=55;
Bacilli|Rep: Formate acetyltransferase - Streptococcus
mutans
Length = 775
Score = 101 bits (242), Expect = 2e-20
Identities = 45/81 (55%), Positives = 58/81 (71%)
Frame = -1
Query: 509 ELRMVRFLRTPEYDELFSGDPIWATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPN 330
+LR V+F RT YDEL+SGDP + T S+ GMG DGR VTK +RFLNTL +G +PEPN
Sbjct: 306 KLRTVKFARTKAYDELYSGDPTFITTSMAGMGADGRHRVTKMDYRFLNTLDNIGNAPEPN 365
Query: 329 MTILWSEKLPLNFKKFAAKVS 267
+T+LWS KLP F+ + +S
Sbjct: 366 LTVLWSSKLPYPFRHYCMSMS 386
Score = 60.5 bits (140), Expect = 5e-08
Identities = 32/89 (35%), Positives = 50/89 (56%), Gaps = 5/89 (5%)
Frame = -3
Query: 267 HRHLSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQ-----MQFFGARANLAKTMLYAI 103
H+H S+QYE M + + I+CCVSP+ + +Q+FGAR N+ K +L +
Sbjct: 387 HKHSSIQYEGVTTMAKEGYGEMSCISCCVSPLDPENEDRRHNLQYFGARVNVLKALLTGL 446
Query: 102 NGGVDEKLKMQVGPKSEPIKGDVLNYDEV 16
NGG D+ K EPI+ +VL+++ V
Sbjct: 447 NGGYDDVHKDYKVFDVEPIRDEVLDFETV 475
Score = 44.0 bits (99), Expect = 0.005
Identities = 22/54 (40%), Positives = 27/54 (50%)
Frame = -2
Query: 670 WT*FGYLAAVKSQNXAAMSSXRTSTFLDVYIERDLKAXKITEQEAQEMVDHLVM 509
W ++A + N AA S R LD++ ERDL TE E QE VD VM
Sbjct: 252 WINIAFMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTESEIQEFVDDFVM 305
>UniRef50_A4E923 Cluster: Putative uncharacterized protein; n=1;
Collinsella aerofaciens ATCC 25986|Rep: Putative
uncharacterized protein - Collinsella aerofaciens ATCC
25986
Length = 652
Score = 69.3 bits (162), Expect = 1e-10
Identities = 40/115 (34%), Positives = 60/115 (52%)
Frame = +1
Query: 13 HHFIIVQDIAFDRFRLRTNLHFQFFVNAAVDCVQHGFRQVCTRTEELHLFTNDHRAYAAS 192
H+ ++++ IA D L +L VN AV+CVQ QV EELHL + H +AA
Sbjct: 167 HNVVVIERIALDGAILGADLLLGDLVNTAVECVQQALGQVGASAEELHLLADAHGGHAAG 226
Query: 193 NSVVIVVEVRTHQVIVLILQREVSMDTLAANFLKFSGSFSDHRMVMFGSGDGPMV 357
++VV+ + + H+V+VL+L R S +L A L+ G + G G G V
Sbjct: 227 DAVVVAM-IDAHEVVVLVLDRGASDRSLGAELLEVLGQTGRPQNGHVGLGAGAHV 280
Score = 60.9 bits (141), Expect = 4e-08
Identities = 35/86 (40%), Positives = 44/86 (51%), Gaps = 1/86 (1%)
Frame = +3
Query: 252 ERGV-DGHFSGEFLEVQRQFFRPQNGHVRFRRRTHGVQGVQETEAVFGNQGTTVEAHTTD 428
+RG D E LEV Q RPQNGHV H ++ VQ A GN+ T V+ H D
Sbjct: 245 DRGASDRSLGAELLEVLGQTGRPQNGHVGLGAGAHVLERVQVAVAHLGNERTAVDTHAAD 304
Query: 429 RFGCPDRVAREQFIVFRSTQEANHTQ 506
G P RVA EQ +V T + +H +
Sbjct: 305 GLGDPLRVAGEQGLVLGGTGKLDHAK 330
>UniRef50_Q7NEY2 Cluster: Glr3745 protein; n=1; Gloeobacter
violaceus|Rep: Glr3745 protein - Gloeobacter violaceus
Length = 460
Score = 65.3 bits (152), Expect = 2e-09
Identities = 27/56 (48%), Positives = 40/56 (71%)
Frame = -1
Query: 422 GMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVSIDTS 255
G+ +GR LVT+ FRF+NT Y +GP+ EP++ +LWS L F++F +V+IDTS
Sbjct: 302 GVDPEGRPLVTRTDFRFVNTCYNLGPAAEPHLLVLWSAALSGAFRRFCGEVTIDTS 357
Score = 39.5 bits (88), Expect = 0.11
Identities = 21/54 (38%), Positives = 27/54 (50%)
Frame = -2
Query: 670 WT*FGYLAAVKSQNXAAMSSXRTSTFLDVYIERDLKAXKITEQEAQEMVDHLVM 509
WT LA S TF DVY +RDL + E+EAQE+VD L++
Sbjct: 230 WTYLALLATRDPLFGRRRSVTGLDTFFDVYFQRDLARGLLVEEEAQELVDDLMI 283
>UniRef50_Q18AX8 Cluster: Glycerol dehydratase; n=5;
Clostridiaceae|Rep: Glycerol dehydratase - Clostridium
difficile (strain 630)
Length = 790
Score = 40.7 bits (91), Expect = 0.047
Identities = 21/66 (31%), Positives = 34/66 (51%)
Frame = -2
Query: 661 FGYLAAVKSQNXAAMSSXRTSTFLDVYIERDLKAXKITEQEAQEMVDHLVMNCVWFASCV 482
F L + S+N A++ R ++ Y E D + KITE EAQE+++ L + + +
Sbjct: 266 FVQLGGILSENPLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTI 325
Query: 481 LRNTMN 464
NT N
Sbjct: 326 SANTAN 331
>UniRef50_O06962 Cluster: Hypothetical pyruvate formate-lyase; n=1;
Salmonella typhimurium|Rep: Hypothetical pyruvate
formate-lyase - Salmonella typhimurium
Length = 276
Score = 40.7 bits (91), Expect = 0.047
Identities = 18/33 (54%), Positives = 23/33 (69%)
Frame = -3
Query: 762 HRGSGFRLKXMAAKYGYDISGPATNGSEAIHGL 664
HR + +++ MAAKYGYDIS PA N EA+ L
Sbjct: 230 HRRALLQMQEMAAKYGYDISRPARNAQEAVQWL 262
>UniRef50_A6TKL5 Cluster: Pyruvate formate-lyase; n=1; Alkaliphilus
metalliredigens QYMF|Rep: Pyruvate formate-lyase -
Alkaliphilus metalliredigens QYMF
Length = 806
Score = 37.1 bits (82), Expect = 0.58
Identities = 21/64 (32%), Positives = 35/64 (54%)
Frame = -3
Query: 204 DYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPKSEPIKGDVLNY 25
DY I CV G Q + G N+A+ + A+N GVD Q+GP++ + D+ ++
Sbjct: 441 DYRIVGCVELAPRGFQGRVNGGFLNVARVVDLALNNGVDRLTNEQIGPQTGAPE-DLKDF 499
Query: 24 DEVM 13
D+V+
Sbjct: 500 DDVL 503
>UniRef50_Q896R2 Cluster: Formate acetyltransferase 2; n=5;
Bacteria|Rep: Formate acetyltransferase 2 - Clostridium
tetani
Length = 803
Score = 35.9 bits (79), Expect = 1.4
Identities = 27/67 (40%), Positives = 34/67 (50%), Gaps = 2/67 (2%)
Frame = -3
Query: 204 DYAIACCVSPMIVGKQMQFFGAR-ANLAKTMLYAINGG-VDEKLKMQVGPKSEPIKGDVL 31
DYAI CV P GK ++ A N+AK + IN G VD K Q+GPK+
Sbjct: 432 DYAIVGCVEPQKPGKTDGWYDAAFFNMAKVLEMTINNGKVDGK---QIGPKTGEFT-SFS 487
Query: 30 NYDEVME 10
N DE +E
Sbjct: 488 NIDEFIE 494
>UniRef50_Q4RTJ3 Cluster: Chromosome 2 SCAF14997, whole genome
shotgun sequence; n=1; Tetraodon nigroviridis|Rep:
Chromosome 2 SCAF14997, whole genome shotgun sequence -
Tetraodon nigroviridis (Green puffer)
Length = 854
Score = 33.9 bits (74), Expect = 5.5
Identities = 28/115 (24%), Positives = 45/115 (39%)
Frame = +1
Query: 28 VQDIAFDRFRLRTNLHFQFFVNAAVDCVQHGFRQVCTRTEELHLFTNDHRAYAASNSVVI 207
+++ +R +R N H + AV V+H ++CT + HL T R A S+
Sbjct: 44 LENYGLERIEVRDNGHGIKAADTAVMAVRHFTSKICTHEDLGHLETYGFRG-EALGSICA 102
Query: 208 VVEVRTHQVIVLILQREVSMDTLAANFLKFSGSFSDHRMVMFGSGDGPMVYRVFR 372
V EV V + + D L +G + G G V R+F+
Sbjct: 103 VAEV----TFVTVTTKTEEEDISTQYTLNLTGGIVSQKPSHLGQGTTVSVLRIFK 153
>UniRef50_Q0CS91 Cluster: Protein TOXD; n=2; Aspergillus|Rep:
Protein TOXD - Aspergillus terreus (strain NIH 2624)
Length = 698
Score = 33.9 bits (74), Expect = 5.5
Identities = 29/105 (27%), Positives = 47/105 (44%), Gaps = 4/105 (3%)
Frame = +3
Query: 222 DASGHRSHTAERGVDGHFSGEFLEVQRQFFRPQNGHVRFRRRTHGV---QGVQETEAVFG 392
+A H+ + ERG +G +FL+ + PQ G VRF + + G E F
Sbjct: 354 EAVVHKMYLGERGFEGCARADFLDELVKSL-PQ-GTVRFSKNLVDIVDEDGASEVRLKF- 410
Query: 393 NQGTTVEAHTTDRFGCPD-RVAREQFIVFRSTQEANHTQFMTRWS 524
+ G+T AH GC R QF++ Q A+H + +++
Sbjct: 411 SDGSTASAHIV--IGCDGIRSKVRQFVIGGDDQPAHHPHYTHKYA 453
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 896,896,273
Number of Sequences: 1657284
Number of extensions: 19401051
Number of successful extensions: 50118
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 47845
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 50095
length of database: 575,637,011
effective HSP length: 100
effective length of database: 409,908,611
effective search space used: 78292544701
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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