BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ps4M0022.Seq (839 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g22390.1 68418.m02612 expressed protein 29 3.8 At1g04850.1 68414.m00481 ubiquitin-associated (UBA)/TS-N domain-... 29 3.8 At1g40129.1 68414.m04766 hypothetical protein 29 5.1 At1g72520.1 68414.m08386 lipoxygenase, putative similar to lipox... 28 6.7 At5g45900.1 68418.m05645 autophagy 7 (APG7) nearly identical to ... 28 8.9 >At5g22390.1 68418.m02612 expressed protein Length = 202 Score = 29.1 bits (62), Expect = 3.8 Identities = 13/24 (54%), Positives = 15/24 (62%) Frame = +1 Query: 502 RRQRSGKSFLFCLSVRVPWNPIEG 573 R+ S KSFL LS PWNP +G Sbjct: 15 RQDNSPKSFLDTLSSSSPWNPSKG 38 >At1g04850.1 68414.m00481 ubiquitin-associated (UBA)/TS-N domain-containing protein weak similarity to SP|P45974 Ubiquitin carboxyl-terminal hydrolase 5 (EC 3.1.2.15) {Homo sapiens}; contains Pfam profile PF00627: UBA/TS-N domain Length = 413 Score = 29.1 bits (62), Expect = 3.8 Identities = 15/37 (40%), Positives = 21/37 (56%), Gaps = 3/37 (8%) Frame = -3 Query: 348 ATSRRALINKHCTSATCEC---VERDLHGKQSGGFRF 247 A S A++N CT+ T C +E DLH K++G F Sbjct: 34 AESTEAVLNLVCTTCTKPCRSKIESDLHTKRTGHTEF 70 >At1g40129.1 68414.m04766 hypothetical protein Length = 567 Score = 28.7 bits (61), Expect = 5.1 Identities = 16/43 (37%), Positives = 22/43 (51%) Frame = -3 Query: 750 PLPTXILSTRGSSXWRPAADMGTNRRDISTYIXHLNFQVRREY 622 P T TRG + WRP TN R+IST+ + R++Y Sbjct: 342 PDATLPFKTRGCTLWRPIKK--TNDREISTHHMSQSRDPRKDY 382 >At1g72520.1 68414.m08386 lipoxygenase, putative similar to lipoxygenase gi:1495804 [Solanum tuberosum], gi:1654140 [Lycopersicon esculentum], GB:CAB56692 [Arabidopsis thaliana] Length = 926 Score = 28.3 bits (60), Expect = 6.7 Identities = 14/33 (42%), Positives = 19/33 (57%) Frame = -1 Query: 629 ESIRTPPQMRCSSRSEPYLPSIGFHGTRTLRQK 531 +S + P R ++PYLPS G RTLR+K Sbjct: 215 QSQKDHPSKRILFTNQPYLPSETPSGLRTLREK 247 >At5g45900.1 68418.m05645 autophagy 7 (APG7) nearly identical to autophagy 7 [Arabidopsis thaliana] GI:19912147; contains Pfam profile PF00899: ThiF family Length = 697 Score = 27.9 bits (59), Expect = 8.9 Identities = 12/31 (38%), Positives = 20/31 (64%) Frame = -2 Query: 637 SPQRVSGHRRKCGALRVPNHISLL*DSMELE 545 SP ++G CG +V NH++LL +S+ L+ Sbjct: 41 SPISITGFYGPCGHPQVSNHLTLLSESLPLD 71 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 17,632,848 Number of Sequences: 28952 Number of extensions: 370864 Number of successful extensions: 960 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 928 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 959 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1941125600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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