BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0996 (691 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g44050.1 68416.m04718 kinesin motor protein-related KLP2 prot... 33 0.14 At3g30550.1 68416.m03866 hypothetical protein includes At2g05890... 32 0.31 At3g27710.1 68416.m03460 zinc finger protein-related contains si... 32 0.41 At3g27720.1 68416.m03461 zinc finger protein-related contains Pf... 31 0.95 At4g39510.1 68417.m05587 cytochrome P450 family protein contains... 30 1.7 At4g04190.2 68417.m00595 expressed protein 30 1.7 At4g04190.1 68417.m00594 expressed protein 30 1.7 At2g17260.1 68415.m01993 glutamate receptor family protein (GLR3... 30 1.7 At3g59690.1 68416.m06660 calmodulin-binding family protein simil... 29 3.8 At5g63450.1 68418.m07965 cytochrome P450, putative 28 5.1 At4g35290.2 68417.m05015 glutamate receptor family protein (GLR3... 28 6.7 At4g35290.1 68417.m05014 glutamate receptor family protein (GLR3... 28 6.7 At4g13040.1 68417.m02035 AP2 domain-containing transcription fac... 28 6.7 At5g57180.2 68418.m07143 expressed protein ; supporting cDNA gi|... 27 8.9 At5g57180.1 68418.m07142 expressed protein ; supporting cDNA gi|... 27 8.9 At5g47730.1 68418.m05897 SEC14 cytosolic factor, putative / poly... 27 8.9 At4g00620.1 68417.m00086 tetrahydrofolate dehydrogenase/cyclohyd... 27 8.9 At3g13540.1 68416.m01702 myb family transcription factor contain... 27 8.9 At2g32390.1 68415.m03958 glutamate receptor family protein (GLR3... 27 8.9 At1g35490.1 68414.m04403 bZIP family transcription factor 27 8.9 >At3g44050.1 68416.m04718 kinesin motor protein-related KLP2 protein, Xenopus laevis, PIR:T30335 Length = 1229 Score = 33.5 bits (73), Expect = 0.14 Identities = 23/74 (31%), Positives = 36/74 (48%), Gaps = 3/74 (4%) Frame = +3 Query: 3 KMKT-VQVILCLFVASLYANETSVSDSKLEDDLYNSI--LVADYDHSVEKSKQIYEDKKS 173 ++KT VQ I C+ A+ET++ SK DDL I L+ D + +E +Q+ E+ S Sbjct: 714 EVKTMVQAIACVSQREAEAHETAIKLSKENDDLRQKIKVLIEDNNKLIELYEQVAEENSS 773 Query: 174 EVITNVVNKLIRNN 215 + NN Sbjct: 774 RAWGKIETDSSSNN 787 >At3g30550.1 68416.m03866 hypothetical protein includes At2g05890, At4g07450, At3g30630, At3g43100, At2g09960, At3g30550, At1g39430, At2g10460, At4g03640, At5g35250 Length = 509 Score = 32.3 bits (70), Expect = 0.31 Identities = 19/66 (28%), Positives = 38/66 (57%), Gaps = 1/66 (1%) Frame = +3 Query: 3 KMKTVQVILCLFVASLYANETSVSDSKL-EDDLYNSILVADYDHSVEKSKQIYEDKKSEV 179 K KT Q +LC+ +++ S + +++ D++ + I+ DYD V+ S I ++ S + Sbjct: 398 KGKTFQFLLCVQRENIFGGYDSFTVARVYTDNIADEIVQEDYDAYVDPSSLISIEQDSLM 457 Query: 180 ITNVVN 197 +TN V+ Sbjct: 458 LTNGVD 463 >At3g27710.1 68416.m03460 zinc finger protein-related contains similarity to zinc finger proteins and Pfam domain, PF01485: IBR domain Length = 537 Score = 31.9 bits (69), Expect = 0.41 Identities = 17/68 (25%), Positives = 34/68 (50%) Frame = +3 Query: 51 YANETSVSDSKLEDDLYNSILVADYDHSVEKSKQIYEDKKSEVITNVVNKLIRNNKMNCM 230 Y + + KLED L SIL +S K ++++ K+ +T+ VN+L + ++ Sbjct: 353 YRYKAHIDSLKLEDKLRKSILEKAVSNSETKDQKVF--KEYSWVTDAVNRLFISRRILSQ 410 Query: 231 EYAYQLWL 254 Y + ++ Sbjct: 411 SYPFAFYM 418 >At3g27720.1 68416.m03461 zinc finger protein-related contains Pfam:PF01485 IBR domain Length = 493 Score = 30.7 bits (66), Expect = 0.95 Identities = 18/68 (26%), Positives = 34/68 (50%) Frame = +3 Query: 51 YANETSVSDSKLEDDLYNSILVADYDHSVEKSKQIYEDKKSEVITNVVNKLIRNNKMNCM 230 Y + + KLED L SIL +S K ++++ K+ I + VN+L R+ ++ Sbjct: 313 YRYKAHIDSLKLEDKLKKSILKKAVLNSETKDQKVF--KEYSWIIDAVNRLFRSRRILSY 370 Query: 231 EYAYQLWL 254 Y + ++ Sbjct: 371 SYPFVFYM 378 >At4g39510.1 68417.m05587 cytochrome P450 family protein contains Pfam PF00067: Cytochrome P450; similar to Cytochrome P450 86A2 (SP:O23066) [Arabidopsis thaliana] Length = 508 Score = 29.9 bits (64), Expect = 1.7 Identities = 22/57 (38%), Positives = 30/57 (52%), Gaps = 1/57 (1%) Frame = +2 Query: 317 NNIKLMYKRDGLALTLDDENSNDGRLAYG-DGKDKTSPKVSWKFVPLWENNKVYFKI 484 ++IKL + L DD+ D LA+ G+D TS +SW F L EN +V KI Sbjct: 281 SHIKLDTTKYELLNPSDDKFLRDTILAFNLAGRDTTSSALSWFFWLLSENPQVVTKI 337 >At4g04190.2 68417.m00595 expressed protein Length = 184 Score = 29.9 bits (64), Expect = 1.7 Identities = 19/60 (31%), Positives = 28/60 (46%), Gaps = 3/60 (5%) Frame = +1 Query: 436 LEVRSSVGEQQ-GLLQDCEHSA*SVLTLAVQTTPNHNHM--AYGANSVEGFKAQWTLAAR 606 L VR E + GL E + + L + T H+ A GA +EGF+ +W+L R Sbjct: 81 LRVRLQESEHKLGLSMPIELAKERITQLEAEATSFERHLILASGAEGIEGFRRRWSLHGR 140 >At4g04190.1 68417.m00594 expressed protein Length = 184 Score = 29.9 bits (64), Expect = 1.7 Identities = 19/60 (31%), Positives = 28/60 (46%), Gaps = 3/60 (5%) Frame = +1 Query: 436 LEVRSSVGEQQ-GLLQDCEHSA*SVLTLAVQTTPNHNHM--AYGANSVEGFKAQWTLAAR 606 L VR E + GL E + + L + T H+ A GA +EGF+ +W+L R Sbjct: 81 LRVRLQESEHKLGLSMPIELAKERITQLEAEATSFERHLILASGAEGIEGFRRRWSLHGR 140 >At2g17260.1 68415.m01993 glutamate receptor family protein (GLR3.1) (GLR2) identical to putative glutamate receptor GLR2 [Arabidopsis thaliana] gi|4185740|gb|AAD09174; plant glutamate receptor family, PMID:11379626 Length = 951 Score = 29.9 bits (64), Expect = 1.7 Identities = 15/35 (42%), Positives = 22/35 (62%) Frame = +2 Query: 344 DGLALTLDDENSNDGRLAYGDGKDKTSPKVSWKFV 448 D +AL DD+NS +G A GD ++ K+S+K V Sbjct: 194 DVVALYNDDDNSRNGVTALGDELEERRCKISYKAV 228 >At3g59690.1 68416.m06660 calmodulin-binding family protein similar to SF16 protein [Helianthus annuus] GI:560150; contains Pfam profile PF00612: IQ calmodulin-binding motif Length = 517 Score = 28.7 bits (61), Expect = 3.8 Identities = 16/69 (23%), Positives = 34/69 (49%) Frame = +3 Query: 60 ETSVSDSKLEDDLYNSILVADYDHSVEKSKQIYEDKKSEVITNVVNKLIRNNKMNCMEYA 239 E + K + L +S + D+D SV + +++K + ++ +I+ + Y+ Sbjct: 236 ENRARNDKDDTKLVSSRMSDDWDDSV-----LTKEEKDVRLHRKIDAMIKRERSMAYAYS 290 Query: 240 YQLWLKAPK 266 +QLW +PK Sbjct: 291 HQLWKNSPK 299 >At5g63450.1 68418.m07965 cytochrome P450, putative Length = 510 Score = 28.3 bits (60), Expect = 5.1 Identities = 13/33 (39%), Positives = 21/33 (63%) Frame = +2 Query: 407 GKDKTSPKVSWKFVPLWENNKVYFKIVNTQRNQ 505 G+D TS ++W F L +N+ V KI++ RN+ Sbjct: 306 GRDTTSAAMTWLFWLLSQNDDVETKILDELRNK 338 >At4g35290.2 68417.m05015 glutamate receptor family protein (GLR3.2) (GLUR2) identical to putative glutamate receptor like-protein GLUR2 [Arabidopsis thaliana] gi|13160471|gb|AAK13248; plant glutamate receptor family, PMID:11379626 Length = 912 Score = 27.9 bits (59), Expect = 6.7 Identities = 14/33 (42%), Positives = 20/33 (60%) Frame = +2 Query: 350 LALTLDDENSNDGRLAYGDGKDKTSPKVSWKFV 448 +AL DD+NS +G A GD + K+S+K V Sbjct: 166 IALYNDDDNSRNGITALGDELEGRRCKISYKAV 198 >At4g35290.1 68417.m05014 glutamate receptor family protein (GLR3.2) (GLUR2) identical to putative glutamate receptor like-protein GLUR2 [Arabidopsis thaliana] gi|13160471|gb|AAK13248; plant glutamate receptor family, PMID:11379626 Length = 912 Score = 27.9 bits (59), Expect = 6.7 Identities = 14/33 (42%), Positives = 20/33 (60%) Frame = +2 Query: 350 LALTLDDENSNDGRLAYGDGKDKTSPKVSWKFV 448 +AL DD+NS +G A GD + K+S+K V Sbjct: 166 IALYNDDDNSRNGITALGDELEGRRCKISYKAV 198 >At4g13040.1 68417.m02035 AP2 domain-containing transcription factor family protein contains Pfam profile PF00847: AP2 domain Length = 226 Score = 27.9 bits (59), Expect = 6.7 Identities = 11/45 (24%), Positives = 23/45 (51%) Frame = +1 Query: 73 PTPNSKTIFTTASSLPITTIPLKRANRSTRTRRAKSSQMS*TNSY 207 P PN++ + + +TT+ +++ R+RR +S M + Y Sbjct: 36 PNPNARAAYNPGPAETVTTVIVEKKAIEERSRRTRSKHMHFRSDY 80 >At5g57180.2 68418.m07143 expressed protein ; supporting cDNA gi|13991645|gb|AF359387.1|AF359387 Length = 435 Score = 27.5 bits (58), Expect = 8.9 Identities = 19/48 (39%), Positives = 27/48 (56%), Gaps = 6/48 (12%) Frame = +1 Query: 61 KPQSPTPNSKTIFTTASSLPITTI------PLKRANRSTRTRRAKSSQ 186 K +SP P+S T T A+S P +TI PL + R RT+R + +Q Sbjct: 21 KVKSPPPSSSTTTTRATS-PSSTISESSNSPLAISTRKPRTQRKRPNQ 67 >At5g57180.1 68418.m07142 expressed protein ; supporting cDNA gi|13991645|gb|AF359387.1|AF359387 Length = 424 Score = 27.5 bits (58), Expect = 8.9 Identities = 19/48 (39%), Positives = 27/48 (56%), Gaps = 6/48 (12%) Frame = +1 Query: 61 KPQSPTPNSKTIFTTASSLPITTI------PLKRANRSTRTRRAKSSQ 186 K +SP P+S T T A+S P +TI PL + R RT+R + +Q Sbjct: 21 KVKSPPPSSSTTTTRATS-PSSTISESSNSPLAISTRKPRTQRKRPNQ 67 >At5g47730.1 68418.m05897 SEC14 cytosolic factor, putative / polyphosphoinositide-binding protein, putative similar to polyphosphoinositide binding protein SEC14 homolog Ssh1p (GI:2739044) {Glycine max} Length = 341 Score = 27.5 bits (58), Expect = 8.9 Identities = 12/31 (38%), Positives = 19/31 (61%) Frame = +2 Query: 314 ENNIKLMYKRDGLALTLDDENSNDGRLAYGD 406 E+ + R+GLA+++DD S+ LA GD Sbjct: 310 ESELHKFENRNGLAVSIDDRKSSHETLANGD 340 >At4g00620.1 68417.m00086 tetrahydrofolate dehydrogenase/cyclohydrolase, putative similar to SP|P07245 C-1-tetrahydrofolate synthase, cytoplasmic (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate synthetase (EC 6.3.4.3)] {Saccharomyces cerevisiae}; contains Pfam profiles PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain, PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain Length = 360 Score = 27.5 bits (58), Expect = 8.9 Identities = 12/29 (41%), Positives = 21/29 (72%), Gaps = 3/29 (10%) Frame = -1 Query: 409 AIAVGKSAIV---AVLIVQRQSETVALVH 332 A+ +G+S IV A L++QR+ TV+++H Sbjct: 230 AVVIGRSNIVGMPAALLLQREDATVSIIH 258 >At3g13540.1 68416.m01702 myb family transcription factor contains Pfam profile: PF00249 myb-like DNA-binding domain Length = 249 Score = 27.5 bits (58), Expect = 8.9 Identities = 15/53 (28%), Positives = 29/53 (54%) Frame = +3 Query: 39 VASLYANETSVSDSKLEDDLYNSILVADYDHSVEKSKQIYEDKKSEVITNVVN 197 ++S + ++T+V+ + D NSI V +H E Y+DK S + +++N Sbjct: 163 ISSSHTDDTTVNGG--DGDSKNSINVFGGEHGYEDFGFCYDDKFSSFLNSLIN 213 >At2g32390.1 68415.m03958 glutamate receptor family protein (GLR3.5) plant glutamate receptor family, PMID:11379626 Length = 895 Score = 27.5 bits (58), Expect = 8.9 Identities = 15/46 (32%), Positives = 25/46 (54%), Gaps = 2/46 (4%) Frame = +2 Query: 341 RDGLALTLDDENSNDGRLAYGDGKDKTSPKVSWK--FVPLWENNKV 472 R+ +A+ +DDE +G GD K K+S+K F P +N+ + Sbjct: 123 REVVAIFVDDEYGRNGISVLGDALAKKRAKISYKAAFPPGADNSSI 168 >At1g35490.1 68414.m04403 bZIP family transcription factor Length = 300 Score = 27.5 bits (58), Expect = 8.9 Identities = 17/74 (22%), Positives = 34/74 (45%) Frame = +1 Query: 46 LCMPTKPQSPTPNSKTIFTTASSLPITTIPLKRANRSTRTRRAKSSQMS*TNSYETTR*T 225 L P P+ + ++ TA+ L +P K + + + + ++ + +NSYE Sbjct: 61 LSEPASPKINKGHRRSASDTAAYLNSALMPSKENHVAGSSWQFQNYDLWQSNSYEQHNKL 120 Query: 226 AWSTPTSYGSRLQR 267 W T+ G+ +QR Sbjct: 121 GWDFSTANGTNIQR 134 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,885,765 Number of Sequences: 28952 Number of extensions: 247761 Number of successful extensions: 892 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 865 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 892 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1467502800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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