BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0970 (685 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC364.07 ||SPCC4G3.01|D-3 phosphoglycerate dehydrogenase |Schi... 59 5e-10 SPACUNK4.10 |||hydroxyacid dehydrogenase |Schizosaccharomyces po... 58 2e-09 SPBC1773.17c ||SPBP26C9.01c|hydroxyacid dehydrogenase |Schizosac... 53 4e-08 SPAC186.07c |||hydroxyacid dehydrogenase |Schizosaccharomyces po... 50 3e-07 SPAC186.02c |||hydroxyacid dehydrogenase |Schizosaccharomyces po... 46 4e-06 SPBC23G7.08c |rga7||GTPase activating protein Rga7|Schizosacchar... 27 1.9 SPAC13G6.11c |erg12||mevalonate kinase Erg12 |Schizosaccharomyce... 26 4.4 SPAC25B8.06c |||serine-tRNA ligase|Schizosaccharomyces pombe|chr... 26 5.8 SPAC6B12.02c |mus7||DNA repair protein Mus7|Schizosaccharomyces ... 25 7.7 >SPCC364.07 ||SPCC4G3.01|D-3 phosphoglycerate dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 466 Score = 59.3 bits (137), Expect = 5e-10 Identities = 34/89 (38%), Positives = 49/89 (55%), Gaps = 10/89 (11%) Frame = +3 Query: 9 GVKIINVGRGGLIQETDFLQALKSGKVGGAALDVFEQEPP-------TDPV---TLEIIQ 158 G +IN RG ++ + A KSGK+ GAA+DV+ EP D + T E+ Sbjct: 276 GSYLINASRGTVVDIPALVDASKSGKIAGAAIDVYPSEPAGNGKDKFVDSLNSWTSELTH 335 Query: 159 QPAVIATPHLGASTKEAQVRVGQEIAEQL 245 +I TPH+G ST+EAQ +G E++E L Sbjct: 336 CKNIILTPHIGGSTEEAQYNIGIEVSEAL 364 >SPACUNK4.10 |||hydroxyacid dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 334 Score = 57.6 bits (133), Expect = 2e-09 Identities = 30/72 (41%), Positives = 41/72 (56%) Frame = +3 Query: 6 RGVKIINVGRGGLIQETDFLQALKSGKVGGAALDVFEQEPPTDPVTLEIIQQPAVIATPH 185 RG+ I+N RG ++ E ++AL G V A LDVFE+EP P LE VI PH Sbjct: 237 RGIVIVNTARGAVMDEAALVEALDEGIVYSAGLDVFEEEPKIHPGLLE---NEKVILLPH 293 Query: 186 LGASTKEAQVRV 221 LG ++ E Q ++ Sbjct: 294 LGTNSLETQYKM 305 >SPBC1773.17c ||SPBP26C9.01c|hydroxyacid dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 340 Score = 52.8 bits (121), Expect = 4e-08 Identities = 30/64 (46%), Positives = 35/64 (54%) Frame = +3 Query: 9 GVKIINVGRGGLIQETDFLQALKSGKVGGAALDVFEQEPPTDPVTLEIIQQPAVIATPHL 188 GV IIN RG +I E F++A+KSGKV A LDVF EP + LE V PH Sbjct: 243 GVYIINTARGAIINEDAFIKAIKSGKVARAGLDVFLNEPTPNKFWLEC---DKVTIQPHC 299 Query: 189 GAST 200 G T Sbjct: 300 GVYT 303 >SPAC186.07c |||hydroxyacid dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 332 Score = 50.0 bits (114), Expect = 3e-07 Identities = 21/39 (53%), Positives = 30/39 (76%) Frame = +3 Query: 6 RGVKIINVGRGGLIQETDFLQALKSGKVGGAALDVFEQE 122 +GVKIIN RGGL+ ++A++SG+VGG A+DV+E E Sbjct: 226 KGVKIINTSRGGLVDTKALVKAIESGQVGGCAMDVYEGE 264 >SPAC186.02c |||hydroxyacid dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 332 Score = 46.4 bits (105), Expect = 4e-06 Identities = 20/39 (51%), Positives = 27/39 (69%) Frame = +3 Query: 6 RGVKIINVGRGGLIQETDFLQALKSGKVGGAALDVFEQE 122 +GV I+N RGGLI + A+ SG+VGG A+DV+E E Sbjct: 226 KGVTIVNTSRGGLIDTKALVDAIDSGQVGGCAIDVYEGE 264 >SPBC23G7.08c |rga7||GTPase activating protein Rga7|Schizosaccharomyces pombe|chr 2|||Manual Length = 695 Score = 27.5 bits (58), Expect = 1.9 Identities = 18/55 (32%), Positives = 26/55 (47%) Frame = -2 Query: 240 AQQSPGQLSPELP**RPPDVEWQLQQAAE*SPK*QDRSAAPARTRPEQPRPPFQT 76 AQQSP L P LP + ++ + A S +R+A+P R P+P T Sbjct: 445 AQQSPPVLLPTLPPIQTTTIQTSREVAPPPSSINSNRAASPFRPTSVSPQPSSPT 499 >SPAC13G6.11c |erg12||mevalonate kinase Erg12 |Schizosaccharomyces pombe|chr 1|||Manual Length = 404 Score = 26.2 bits (55), Expect = 4.4 Identities = 11/32 (34%), Positives = 19/32 (59%) Frame = -1 Query: 148 SKVTGSVGGSCSNTSRAAPPTFPDFRACKKSV 53 +K+TG+ GG C+ T +F+ CK+S+ Sbjct: 323 TKLTGAGGGGCTITLLTPECKEEEFKLCKESL 354 >SPAC25B8.06c |||serine-tRNA ligase|Schizosaccharomyces pombe|chr 1|||Manual Length = 454 Score = 25.8 bits (54), Expect = 5.8 Identities = 10/31 (32%), Positives = 17/31 (54%) Frame = -1 Query: 316 RSLVQDTGDFGQQSRECSRLHRFTSCSAISW 224 R+ ++ G G+++R RLH FT +W Sbjct: 289 RAYRREAGARGKENRGLYRLHEFTKVELFAW 319 >SPAC6B12.02c |mus7||DNA repair protein Mus7|Schizosaccharomyces pombe|chr 1|||Manual Length = 1888 Score = 25.4 bits (53), Expect = 7.7 Identities = 9/17 (52%), Positives = 13/17 (76%) Frame = +1 Query: 499 GFINLRS*FIETYLMIN 549 G +NL S F+ETY ++N Sbjct: 771 GLVNLASSFLETYALVN 787 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,145,785 Number of Sequences: 5004 Number of extensions: 34352 Number of successful extensions: 102 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 101 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 101 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 315915086 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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