Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= prgv0919
(720 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g25660.1 68416.m03194 glutamyl-tRNA(Gln) amidotransferase, pu... 53 2e-07
At1g08980.1 68414.m01001 amidase family protein similar to compo... 48 8e-06
At5g64440.1 68418.m08095 amidase family protein low similarity t... 43 2e-04
At5g09420.1 68418.m01091 chloroplast outer membrane translocon s... 40 0.002
At3g17970.1 68416.m02286 chloroplast outer membrane translocon s... 40 0.002
At5g07360.1 68418.m00840 amidase family protein low similarity t... 39 0.003
At5g37380.2 68418.m04492 DNAJ heat shock N-terminal domain-conta... 29 4.1
At5g37380.1 68418.m04491 DNAJ heat shock N-terminal domain-conta... 29 4.1
At3g50220.1 68416.m05492 expressed protein contains Pfam profile... 28 5.4
At5g60130.1 68418.m07538 transcriptional factor B3 family protei... 27 9.5
At2g01810.1 68415.m00111 PHD finger family protein contains Pfam... 27 9.5
>At3g25660.1 68416.m03194 glutamyl-tRNA(Gln) amidotransferase,
putative similar to SP|O06491 Glutamyl-tRNA(Gln)
amidotransferase subunit A (EC 6.3.5.-) {Bacillus
subtilis}; contains Pfam profile PF01425: Amidase
Length = 537
Score = 53.2 bits (122), Expect = 2e-07
Identities = 22/30 (73%), Positives = 26/30 (86%)
Frame = +3
Query: 165 AIGSDTGGSTRNPAALCGVVGLKPSYGLVS 254
++GSDTGGS R PA+ CGVVGLKP+YG VS
Sbjct: 207 SLGSDTGGSVRQPASFCGVVGLKPTYGRVS 236
Score = 39.9 bits (89), Expect = 0.002
Identities = 18/31 (58%), Positives = 19/31 (61%)
Frame = +1
Query: 1 VGKCNLDEFAMGSGTIDSIFGPTRNPWQYER 93
VGK N+DEF MGS T S F T NPW R
Sbjct: 156 VGKTNMDEFGMGSTTEASAFQVTANPWDLSR 186
Score = 39.1 bits (87), Expect = 0.003
Identities = 25/83 (30%), Positives = 45/83 (54%), Gaps = 8/83 (9%)
Frame = +2
Query: 257 YGLIPLVNSMDVPGILTRDIDDCVAVLNSVAGPDDLDSTTIKRDYKPIEIQ--DID---- 418
+GL+ +S+DV G + D +L++++G D DST+ K+D + Q +D
Sbjct: 238 FGLMAYASSLDVIGCFGSTVADAGMLLHAISGYDRFDSTSSKQDVPEFQSQFLSVDHFES 297
Query: 419 --MSSVKIGIPKEYHCEGMDREV 481
++ VK+GI +E +G+D V
Sbjct: 298 KPLNGVKVGIIRETLEDGVDSGV 320
Score = 31.5 bits (68), Expect = 0.58
Identities = 16/41 (39%), Positives = 24/41 (58%)
Frame = +1
Query: 511 LEKENCIVKQVSLPYSSASIVVYSILKSM*KSQATWARYDG 633
LE CI+ +VSLP S + Y ++ S +S + +RYDG
Sbjct: 331 LEALGCILTEVSLPSFSLGLPAYYVIASS-ESSSNLSRYDG 370
>At1g08980.1 68414.m01001 amidase family protein similar to
component of chloroplast outer membrane translocon Toc64
[Pisum sativum] GI:7453538; contains Pfam profile
PF01425: Amidase; supporting cDNA
gi|11493701|gb|AF202077.1|AF202077
Length = 425
Score = 47.6 bits (108), Expect = 8e-06
Identities = 19/37 (51%), Positives = 28/37 (75%)
Frame = +3
Query: 150 RIMRTAIGSDTGGSTRNPAALCGVVGLKPSYGLVSVM 260
R++ +IG+DTGGS R PA+ CG+ G +PS+G VS +
Sbjct: 124 RLVDFSIGTDTGGSVRVPASYCGIFGFRPSHGAVSTV 160
>At5g64440.1 68418.m08095 amidase family protein low similarity to
enantiomerase-selective amidase [Rhodococcus sp.]
GI:152052; contains Pfam profile PF01425: Amidase
Length = 607
Score = 43.2 bits (97), Expect = 2e-04
Identities = 17/27 (62%), Positives = 22/27 (81%)
Frame = +3
Query: 165 AIGSDTGGSTRNPAALCGVVGLKPSYG 245
A+G+D GGS R P+ALCG+ GLK +YG
Sbjct: 297 ALGTDGGGSVRIPSALCGITGLKTTYG 323
Score = 29.5 bits (63), Expect = 2.3
Identities = 13/31 (41%), Positives = 19/31 (61%)
Frame = +1
Query: 1 VGKCNLDEFAMGSGTIDSIFGPTRNPWQYER 93
+GK N+ E MG+ +S +G TRNP +R
Sbjct: 246 LGKANMHELGMGTTGNNSNYGTTRNPHDPKR 276
>At5g09420.1 68418.m01091 chloroplast outer membrane translocon
subunit, putative similar to component of chloroplast
outer membrane translocon Toc64 [Pisum sativum]
GI:7453538; contains Pfam profiles PF01425: Amidase,
PF00515: TPR Domain
Length = 603
Score = 39.9 bits (89), Expect = 0.002
Identities = 16/30 (53%), Positives = 21/30 (70%)
Frame = +3
Query: 165 AIGSDTGGSTRNPAALCGVVGLKPSYGLVS 254
++G DT G R PAA CG++G +PS G VS
Sbjct: 172 SLGIDTTGGVRVPAAFCGILGFRPSQGTVS 201
>At3g17970.1 68416.m02286 chloroplast outer membrane translocon
subunit, putative similar to Toc64 [Pisum sativum]
GI:7453538; contains Pfam profile PF00515 TPR Domain
Length = 589
Score = 39.9 bits (89), Expect = 0.002
Identities = 17/30 (56%), Positives = 20/30 (66%)
Frame = +3
Query: 165 AIGSDTGGSTRNPAALCGVVGLKPSYGLVS 254
A+G DT G R PA CGV+G K SYG +S
Sbjct: 159 ALGIDTVGGVRVPAGYCGVLGFKSSYGAIS 188
>At5g07360.1 68418.m00840 amidase family protein low similarity to
enantiomerase-selective amidase [Rhodococcus sp.]
GI:152052; contains Pfam profile PF01425: Amidase
Length = 659
Score = 39.1 bits (87), Expect = 0.003
Identities = 16/29 (55%), Positives = 21/29 (72%)
Frame = +3
Query: 165 AIGSDTGGSTRNPAALCGVVGLKPSYGLV 251
AIGS+T GS PAA CG+ L+P++G V
Sbjct: 364 AIGSETAGSMTYPAARCGITALRPTFGSV 392
Score = 31.5 bits (68), Expect = 0.58
Identities = 19/66 (28%), Positives = 34/66 (51%), Gaps = 2/66 (3%)
Frame = +2
Query: 260 GLIPLVNSMDVPGILTRDIDDCVAVLNSVAG--PDDLDSTTIKRDYKPIEIQDIDMSSVK 433
G++ + S+D G R DC +L+++ G PDDL S I + P +D++ +
Sbjct: 396 GVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSSREIAFE-DPF---SVDITKLT 451
Query: 434 IGIPKE 451
+G K+
Sbjct: 452 VGYTKD 457
>At5g37380.2 68418.m04492 DNAJ heat shock N-terminal
domain-containing protein similar to SP|Q9QYI4 DnaJ
homolog subfamily B member 12 {Mus musculus}; contains
Pfam profile PF00226: DnaJ domain
Length = 431
Score = 28.7 bits (61), Expect = 4.1
Identities = 30/105 (28%), Positives = 45/105 (42%)
Frame = +2
Query: 248 GVSYGLIPLVNSMDVPGILTRDIDDCVAVLNSVAGPDDLDSTTIKRDYKPIEIQDIDMSS 427
GVS L L + + D+D +LN A P D D T+KR Y+ + + +
Sbjct: 43 GVSQMLATLDVYIAAENKVNEDVD-WYGILN--ASPRD-DDETLKRKYRKLALMLHPDKN 98
Query: 428 VKIGIPKEYHCEGMDREVIDTWSYVSDFLKKKTVL*NKSLCHIPQ 562
IG EG + V + W ++SD K+ KSL + Q
Sbjct: 99 KSIG------AEGAFKHVSEAWKFLSDKEKRAAYDRRKSLHSVYQ 137
>At5g37380.1 68418.m04491 DNAJ heat shock N-terminal
domain-containing protein similar to SP|Q9QYI4 DnaJ
homolog subfamily B member 12 {Mus musculus}; contains
Pfam profile PF00226: DnaJ domain
Length = 431
Score = 28.7 bits (61), Expect = 4.1
Identities = 30/105 (28%), Positives = 45/105 (42%)
Frame = +2
Query: 248 GVSYGLIPLVNSMDVPGILTRDIDDCVAVLNSVAGPDDLDSTTIKRDYKPIEIQDIDMSS 427
GVS L L + + D+D +LN A P D D T+KR Y+ + + +
Sbjct: 43 GVSQMLATLDVYIAAENKVNEDVD-WYGILN--ASPRD-DDETLKRKYRKLALMLHPDKN 98
Query: 428 VKIGIPKEYHCEGMDREVIDTWSYVSDFLKKKTVL*NKSLCHIPQ 562
IG EG + V + W ++SD K+ KSL + Q
Sbjct: 99 KSIG------AEGAFKHVSEAWKFLSDKEKRAAYDRRKSLHSVYQ 137
>At3g50220.1 68416.m05492 expressed protein contains Pfam profile
PF04669: Protein of unknown function (DUF579)
Length = 322
Score = 28.3 bits (60), Expect = 5.4
Identities = 19/64 (29%), Positives = 34/64 (53%), Gaps = 6/64 (9%)
Frame = -2
Query: 719 PPH*ILVASMTHTILLW-NMSHQ-----F*DHIQYPSYLAHVACDFHIDFSIEYTTIEAE 558
PP +LV +TH LLW +++H ++ Y +Y + + + F ++YTT +A
Sbjct: 122 PPCNLLVFGLTHETLLWKSLNHNGRTVFIEENRYYAAYFEEIHPEIDV-FDVQYTT-KAH 179
Query: 557 EYGK 546
E G+
Sbjct: 180 EAGE 183
>At5g60130.1 68418.m07538 transcriptional factor B3 family protein
contains Pfam profile PF02362: B3 DNA binding domain
Length = 300
Score = 27.5 bits (58), Expect = 9.5
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +3
Query: 36 LRYHRFHIRSNPEPMAVRKK*LENSWWQLWWKRCCRD 146
+ + RF +S PE + VR + + W+L K+CC D
Sbjct: 5 ISFTRFLPKSLPETVTVRS--ISGNIWKLELKKCCGD 39
>At2g01810.1 68415.m00111 PHD finger family protein contains Pfam
profile: PF00628: PHD-finger
Length = 697
Score = 27.5 bits (58), Expect = 9.5
Identities = 11/20 (55%), Positives = 16/20 (80%)
Frame = -2
Query: 476 PYPSPHNDILLVYQSLRMTY 417
P SP++DIL +YQ+L +TY
Sbjct: 479 PGLSPYDDILYLYQNLLLTY 498
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,368,376
Number of Sequences: 28952
Number of extensions: 347573
Number of successful extensions: 872
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 846
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 872
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1565336320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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