BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0915 (693 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1683.07 |mal1||alpha-glucosidase Mal1 |Schizosaccharomyces p... 70 3e-13 SPAC27E2.01 |||alpha-amylase homolog |Schizosaccharomyces pombe|... 45 9e-06 SPCC757.12 |||alpha-amylase homolog |Schizosaccharomyces pombe|c... 38 0.001 SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog Mde5|S... 38 0.001 SPCC11E10.09c ||SPCC188.01c|alpha-amylase homolog |Schizosacchar... 38 0.001 SPAC23D3.14c |aah2||alpha-amylase homolog Aah2|Schizosaccharomyc... 36 0.006 SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 32 0.090 SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosacch... 32 0.090 SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-gluca... 32 0.090 SPCC63.02c |aah3||alpha-amylase homolog Aah3|Schizosaccharomyces... 30 0.36 SPACUNK4.10 |||hydroxyacid dehydrogenase |Schizosaccharomyces po... 30 0.36 SPAC12B10.13 |||CTLH domain|Schizosaccharomyces pombe|chr 1|||Ma... 27 3.4 SPCC16A11.03c |||DUF2009 protein|Schizosaccharomyces pombe|chr 3... 26 4.5 SPAC823.15 |ppa1||minor serine/threonine protein phosphatase Ppa... 26 4.5 >SPBC1683.07 |mal1||alpha-glucosidase Mal1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 579 Score = 70.1 bits (164), Expect = 3e-13 Identities = 35/83 (42%), Positives = 54/83 (65%) Frame = +3 Query: 258 YEVFPASFKDSNNDGTGDFKGLITKLDYIQNLGVAAIRLNYIFQADHYPEDYNNVTSMLD 437 Y+++PASFKDSN DG GD +G+I+K+DY++ L V +I L I+ + Y +V+ Sbjct: 20 YQIYPASFKDSNGDGFGDLEGIISKVDYLKALNVESIWLCPIYPSPLKDMGY-DVSDYKQ 78 Query: 438 IDRSLGVLKDLQDLIFNIHKRNM 506 ID G L+DL L+ +H+R+M Sbjct: 79 IDSRYGTLEDLDRLMKALHERDM 101 >SPAC27E2.01 |||alpha-amylase homolog |Schizosaccharomyces pombe|chr 1|||Manual Length = 491 Score = 45.2 bits (102), Expect = 9e-06 Identities = 25/73 (34%), Positives = 41/73 (56%), Gaps = 6/73 (8%) Frame = +3 Query: 306 GDFKGLITKLDYIQNLGVAAIRLNYIFQ----ADHYPEDYNN--VTSMLDIDRSLGVLKD 467 G ++G+I KLDYIQ+LG A+ ++ I + Y E Y+ + ++ G +D Sbjct: 56 GTWRGIIQKLDYIQSLGCTAVWISPIVKNIEGVTGYGEAYHGYWAEDLTQLNPHFGTKQD 115 Query: 468 LQDLIFNIHKRNM 506 L +L+ +HKRNM Sbjct: 116 LTELVDQLHKRNM 128 >SPCC757.12 |||alpha-amylase homolog |Schizosaccharomyces pombe|chr 3|||Manual Length = 625 Score = 37.9 bits (84), Expect = 0.001 Identities = 29/84 (34%), Positives = 44/84 (52%), Gaps = 7/84 (8%) Frame = +3 Query: 276 SFKDSNNDGTGDFKGLITKLDYIQNLGVAAIRLNYIFQ--ADHYPED---YNNV--TSML 434 SF DS+ G G + G+ +KLDYIQ +G AI ++ + + Y D Y+ T Sbjct: 52 SFDDSSYCG-GTWSGIRSKLDYIQGMGFNAIWISPVEKNLEGSYGSDGEAYHGYWNTDFT 110 Query: 435 DIDRSLGVLKDLQDLIFNIHKRNM 506 ++ G DL DLI ++H R+M Sbjct: 111 QLNEHFGSEDDLIDLITDMHNRDM 134 >SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog Mde5|Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 37.9 bits (84), Expect = 0.001 Identities = 24/73 (32%), Positives = 38/73 (52%), Gaps = 6/73 (8%) Frame = +3 Query: 306 GDFKGLITKLDYIQNLGVAAIRLNYIFQ----ADHYPEDYNNV--TSMLDIDRSLGVLKD 467 G+++G+I KLDYIQ +G AI ++ I + Y E Y+ + ++ G +D Sbjct: 62 GNWRGIIDKLDYIQGMGFTAIWISPIIKNIEGRTKYGEAYHGYWPQDLYTLNPHFGTEQD 121 Query: 468 LQDLIFNIHKRNM 506 L DL +H R M Sbjct: 122 LIDLADALHDRGM 134 >SPCC11E10.09c ||SPCC188.01c|alpha-amylase homolog |Schizosaccharomyces pombe|chr 3|||Manual Length = 478 Score = 37.9 bits (84), Expect = 0.001 Identities = 23/73 (31%), Positives = 40/73 (54%), Gaps = 6/73 (8%) Frame = +3 Query: 306 GDFKGLITKLDYIQNLGVAAIRLNYIFQADHYPED----YNN--VTSMLDIDRSLGVLKD 467 G +KG+ LDYI++LG AI ++ I + D Y+ M ++ + G +D Sbjct: 51 GTWKGITRNLDYIKSLGCTAIWISPIVKNISETTDCGQAYHGYWAQDMTQLNENFGTEED 110 Query: 468 LQDLIFNIHKRNM 506 L++L+ IH++NM Sbjct: 111 LKELVNAIHEKNM 123 >SPAC23D3.14c |aah2||alpha-amylase homolog Aah2|Schizosaccharomyces pombe|chr 1|||Manual Length = 581 Score = 35.9 bits (79), Expect = 0.006 Identities = 21/73 (28%), Positives = 39/73 (53%), Gaps = 6/73 (8%) Frame = +3 Query: 306 GDFKGLITKLDYIQNLGVAAIRLNYIFQ----ADHYPEDYNN--VTSMLDIDRSLGVLKD 467 G + G+ LDYIQ +G AI ++ IF+ D Y+ T++ +++ G ++ Sbjct: 66 GTWNGIRNHLDYIQGMGFDAIWISPIFENVEGNDIDGSSYHGYWTTNLYELNHHFGTKEE 125 Query: 468 LQDLIFNIHKRNM 506 +LI +HKR++ Sbjct: 126 FMELIQELHKRDI 138 >SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|Schizosaccharomyces pombe|chr 1|||Manual Length = 2397 Score = 31.9 bits (69), Expect = 0.090 Identities = 21/84 (25%), Positives = 37/84 (44%), Gaps = 1/84 (1%) Frame = +3 Query: 258 YEVFPASFKDSNNDGTGDFKGLITKLDYIQNLGVAAIRL-NYIFQADHYPEDYNNVTSML 434 Y+++ F++ GD G+ LDY+Q +GV A+ F + D + Sbjct: 91 YDIYETEFRNG-----GDIIGVRQSLDYLQGMGVKAVYFAGTPFVNMPWGADQYSPLDFT 145 Query: 435 DIDRSLGVLKDLQDLIFNIHKRNM 506 +D LG + D + I +H + M Sbjct: 146 LLDPHLGTINDWRGTIEEMHSKGM 169 >SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosaccharomyces pombe|chr 2|||Manual Length = 2358 Score = 31.9 bits (69), Expect = 0.090 Identities = 28/95 (29%), Positives = 43/95 (45%), Gaps = 7/95 (7%) Frame = +3 Query: 258 YEVFPASFKDSNNDGTGDFKGLITKLDYIQNLGVAAIR------LNYIFQADHY-PEDYN 416 Y+++ F++ GD GL LDY++ +G+ I LN + AD Y P DY Sbjct: 90 YDIYETGFRNG-----GDIIGLKDSLDYLEIMGIKVIYIAGTPFLNQPWGADQYSPLDYT 144 Query: 417 NVTSMLDIDRSLGVLKDLQDLIFNIHKRNMSSYLI 521 +D G + +D I IH+R YL+ Sbjct: 145 ------ILDHHSGTIAQWRDTIEEIHRRGF--YLV 171 >SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-glucan synthase Ags1|Schizosaccharomyces pombe|chr 3|||Manual Length = 2410 Score = 31.9 bits (69), Expect = 0.090 Identities = 29/95 (30%), Positives = 43/95 (45%), Gaps = 7/95 (7%) Frame = +3 Query: 258 YEVFPASFKDSNNDGTGDFKGLITKLDYIQNLGVAAIRL------NYIFQADHY-PEDYN 416 Y++ SF++ GD GL LDY++ LG I + N + AD Y P DY Sbjct: 95 YDISEVSFRNG-----GDIVGLELSLDYLEGLGTQGIYIAGTPFVNMPWGADQYSPLDYT 149 Query: 417 NVTSMLDIDRSLGVLKDLQDLIFNIHKRNMSSYLI 521 +D LG + + I +H+R M YL+ Sbjct: 150 ------ILDHHLGTIDQWRSTITAMHERGM--YLV 176 >SPCC63.02c |aah3||alpha-amylase homolog Aah3|Schizosaccharomyces pombe|chr 3|||Manual Length = 564 Score = 29.9 bits (64), Expect = 0.36 Identities = 14/33 (42%), Positives = 22/33 (66%) Frame = +3 Query: 285 DSNNDGTGDFKGLITKLDYIQNLGVAAIRLNYI 383 D+N G +KG+ KLDYI+++G AI ++ I Sbjct: 54 DANQYCGGTWKGIENKLDYIEDMGFNAIWISPI 86 >SPACUNK4.10 |||hydroxyacid dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 334 Score = 29.9 bits (64), Expect = 0.36 Identities = 17/56 (30%), Positives = 28/56 (50%) Frame = +3 Query: 345 QNLGVAAIRLNYIFQADHYPEDYNNVTSMLDIDRSLGVLKDLQDLIFNIHKRNMSS 512 + + VAA I Q H P+ ++ T+ + I LG L+ IF +HK N ++ Sbjct: 92 ETVDVAACTARGI-QVSHVPKAVDDATADVGIFLMLGALRGFNQGIFELHKNNWNA 146 >SPAC12B10.13 |||CTLH domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 240 Score = 26.6 bits (56), Expect = 3.4 Identities = 13/32 (40%), Positives = 19/32 (59%) Frame = -2 Query: 677 LNKNPINSLVLETNEL*QQLLDPNS*LQYYLL 582 + INS + + NEL ++LD NS L + LL Sbjct: 81 IKSGSINSAICKLNELEPEILDTNSELLFELL 112 >SPCC16A11.03c |||DUF2009 protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 466 Score = 26.2 bits (55), Expect = 4.5 Identities = 16/46 (34%), Positives = 24/46 (52%), Gaps = 1/46 (2%) Frame = +1 Query: 19 SKNGDLIIGQAEDVKLNGNLKINNRKL-PSFVNWNWVVIRKILLWV 153 +KNG L IG KLN + K + S V W+ ++ + LLW+ Sbjct: 251 TKNGSLKIGYMTGAKLNHDHKTQFFYVDQSLVFWSCMMDQMFLLWL 296 >SPAC823.15 |ppa1||minor serine/threonine protein phosphatase Ppa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 309 Score = 26.2 bits (55), Expect = 4.5 Identities = 12/42 (28%), Positives = 21/42 (50%) Frame = +3 Query: 306 GDFKGLITKLDYIQNLGVAAIRLNYIFQADHYPEDYNNVTSM 431 GD G L + N+G + NY+F D+ Y++V ++ Sbjct: 56 GDIHGQFHDLMELFNIGGPSPDTNYLFMGDYVDRGYHSVETV 97 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,859,501 Number of Sequences: 5004 Number of extensions: 59265 Number of successful extensions: 157 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 150 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 154 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 321951680 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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