SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= prgv0915
         (693 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1683.07 |mal1||alpha-glucosidase Mal1 |Schizosaccharomyces p...    70   3e-13
SPAC27E2.01 |||alpha-amylase homolog |Schizosaccharomyces pombe|...    45   9e-06
SPCC757.12 |||alpha-amylase homolog |Schizosaccharomyces pombe|c...    38   0.001
SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog Mde5|S...    38   0.001
SPCC11E10.09c ||SPCC188.01c|alpha-amylase homolog |Schizosacchar...    38   0.001
SPAC23D3.14c |aah2||alpha-amylase homolog Aah2|Schizosaccharomyc...    36   0.006
SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S...    32   0.090
SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosacch...    32   0.090
SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-gluca...    32   0.090
SPCC63.02c |aah3||alpha-amylase homolog Aah3|Schizosaccharomyces...    30   0.36 
SPACUNK4.10 |||hydroxyacid dehydrogenase |Schizosaccharomyces po...    30   0.36 
SPAC12B10.13 |||CTLH domain|Schizosaccharomyces pombe|chr 1|||Ma...    27   3.4  
SPCC16A11.03c |||DUF2009 protein|Schizosaccharomyces pombe|chr 3...    26   4.5  
SPAC823.15 |ppa1||minor serine/threonine protein phosphatase Ppa...    26   4.5  

>SPBC1683.07 |mal1||alpha-glucosidase Mal1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 579

 Score = 70.1 bits (164), Expect = 3e-13
 Identities = 35/83 (42%), Positives = 54/83 (65%)
 Frame = +3

Query: 258 YEVFPASFKDSNNDGTGDFKGLITKLDYIQNLGVAAIRLNYIFQADHYPEDYNNVTSMLD 437
           Y+++PASFKDSN DG GD +G+I+K+DY++ L V +I L  I+ +      Y +V+    
Sbjct: 20  YQIYPASFKDSNGDGFGDLEGIISKVDYLKALNVESIWLCPIYPSPLKDMGY-DVSDYKQ 78

Query: 438 IDRSLGVLKDLQDLIFNIHKRNM 506
           ID   G L+DL  L+  +H+R+M
Sbjct: 79  IDSRYGTLEDLDRLMKALHERDM 101


>SPAC27E2.01 |||alpha-amylase homolog |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 491

 Score = 45.2 bits (102), Expect = 9e-06
 Identities = 25/73 (34%), Positives = 41/73 (56%), Gaps = 6/73 (8%)
 Frame = +3

Query: 306 GDFKGLITKLDYIQNLGVAAIRLNYIFQ----ADHYPEDYNN--VTSMLDIDRSLGVLKD 467
           G ++G+I KLDYIQ+LG  A+ ++ I +       Y E Y+      +  ++   G  +D
Sbjct: 56  GTWRGIIQKLDYIQSLGCTAVWISPIVKNIEGVTGYGEAYHGYWAEDLTQLNPHFGTKQD 115

Query: 468 LQDLIFNIHKRNM 506
           L +L+  +HKRNM
Sbjct: 116 LTELVDQLHKRNM 128


>SPCC757.12 |||alpha-amylase homolog |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 625

 Score = 37.9 bits (84), Expect = 0.001
 Identities = 29/84 (34%), Positives = 44/84 (52%), Gaps = 7/84 (8%)
 Frame = +3

Query: 276 SFKDSNNDGTGDFKGLITKLDYIQNLGVAAIRLNYIFQ--ADHYPED---YNNV--TSML 434
           SF DS+  G G + G+ +KLDYIQ +G  AI ++ + +     Y  D   Y+    T   
Sbjct: 52  SFDDSSYCG-GTWSGIRSKLDYIQGMGFNAIWISPVEKNLEGSYGSDGEAYHGYWNTDFT 110

Query: 435 DIDRSLGVLKDLQDLIFNIHKRNM 506
            ++   G   DL DLI ++H R+M
Sbjct: 111 QLNEHFGSEDDLIDLITDMHNRDM 134


>SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog
           Mde5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 513

 Score = 37.9 bits (84), Expect = 0.001
 Identities = 24/73 (32%), Positives = 38/73 (52%), Gaps = 6/73 (8%)
 Frame = +3

Query: 306 GDFKGLITKLDYIQNLGVAAIRLNYIFQ----ADHYPEDYNNV--TSMLDIDRSLGVLKD 467
           G+++G+I KLDYIQ +G  AI ++ I +       Y E Y+      +  ++   G  +D
Sbjct: 62  GNWRGIIDKLDYIQGMGFTAIWISPIIKNIEGRTKYGEAYHGYWPQDLYTLNPHFGTEQD 121

Query: 468 LQDLIFNIHKRNM 506
           L DL   +H R M
Sbjct: 122 LIDLADALHDRGM 134


>SPCC11E10.09c ||SPCC188.01c|alpha-amylase homolog
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 478

 Score = 37.9 bits (84), Expect = 0.001
 Identities = 23/73 (31%), Positives = 40/73 (54%), Gaps = 6/73 (8%)
 Frame = +3

Query: 306 GDFKGLITKLDYIQNLGVAAIRLNYIFQADHYPED----YNN--VTSMLDIDRSLGVLKD 467
           G +KG+   LDYI++LG  AI ++ I +      D    Y+      M  ++ + G  +D
Sbjct: 51  GTWKGITRNLDYIKSLGCTAIWISPIVKNISETTDCGQAYHGYWAQDMTQLNENFGTEED 110

Query: 468 LQDLIFNIHKRNM 506
           L++L+  IH++NM
Sbjct: 111 LKELVNAIHEKNM 123


>SPAC23D3.14c |aah2||alpha-amylase homolog Aah2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 581

 Score = 35.9 bits (79), Expect = 0.006
 Identities = 21/73 (28%), Positives = 39/73 (53%), Gaps = 6/73 (8%)
 Frame = +3

Query: 306 GDFKGLITKLDYIQNLGVAAIRLNYIFQ----ADHYPEDYNN--VTSMLDIDRSLGVLKD 467
           G + G+   LDYIQ +G  AI ++ IF+     D     Y+    T++ +++   G  ++
Sbjct: 66  GTWNGIRNHLDYIQGMGFDAIWISPIFENVEGNDIDGSSYHGYWTTNLYELNHHFGTKEE 125

Query: 468 LQDLIFNIHKRNM 506
             +LI  +HKR++
Sbjct: 126 FMELIQELHKRDI 138


>SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase
           Mok11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 2397

 Score = 31.9 bits (69), Expect = 0.090
 Identities = 21/84 (25%), Positives = 37/84 (44%), Gaps = 1/84 (1%)
 Frame = +3

Query: 258 YEVFPASFKDSNNDGTGDFKGLITKLDYIQNLGVAAIRL-NYIFQADHYPEDYNNVTSML 434
           Y+++   F++      GD  G+   LDY+Q +GV A+      F    +  D  +     
Sbjct: 91  YDIYETEFRNG-----GDIIGVRQSLDYLQGMGVKAVYFAGTPFVNMPWGADQYSPLDFT 145

Query: 435 DIDRSLGVLKDLQDLIFNIHKRNM 506
            +D  LG + D +  I  +H + M
Sbjct: 146 LLDPHLGTINDWRGTIEEMHSKGM 169


>SPBC16D10.05 |mok13||alpha-1,3-glucan synthase
           Mok13|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2358

 Score = 31.9 bits (69), Expect = 0.090
 Identities = 28/95 (29%), Positives = 43/95 (45%), Gaps = 7/95 (7%)
 Frame = +3

Query: 258 YEVFPASFKDSNNDGTGDFKGLITKLDYIQNLGVAAIR------LNYIFQADHY-PEDYN 416
           Y+++   F++      GD  GL   LDY++ +G+  I       LN  + AD Y P DY 
Sbjct: 90  YDIYETGFRNG-----GDIIGLKDSLDYLEIMGIKVIYIAGTPFLNQPWGADQYSPLDYT 144

Query: 417 NVTSMLDIDRSLGVLKDLQDLIFNIHKRNMSSYLI 521
                  +D   G +   +D I  IH+R    YL+
Sbjct: 145 ------ILDHHSGTIAQWRDTIEEIHRRGF--YLV 171


>SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-glucan
           synthase Ags1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 2410

 Score = 31.9 bits (69), Expect = 0.090
 Identities = 29/95 (30%), Positives = 43/95 (45%), Gaps = 7/95 (7%)
 Frame = +3

Query: 258 YEVFPASFKDSNNDGTGDFKGLITKLDYIQNLGVAAIRL------NYIFQADHY-PEDYN 416
           Y++   SF++      GD  GL   LDY++ LG   I +      N  + AD Y P DY 
Sbjct: 95  YDISEVSFRNG-----GDIVGLELSLDYLEGLGTQGIYIAGTPFVNMPWGADQYSPLDYT 149

Query: 417 NVTSMLDIDRSLGVLKDLQDLIFNIHKRNMSSYLI 521
                  +D  LG +   +  I  +H+R M  YL+
Sbjct: 150 ------ILDHHLGTIDQWRSTITAMHERGM--YLV 176


>SPCC63.02c |aah3||alpha-amylase homolog Aah3|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 564

 Score = 29.9 bits (64), Expect = 0.36
 Identities = 14/33 (42%), Positives = 22/33 (66%)
 Frame = +3

Query: 285 DSNNDGTGDFKGLITKLDYIQNLGVAAIRLNYI 383
           D+N    G +KG+  KLDYI+++G  AI ++ I
Sbjct: 54  DANQYCGGTWKGIENKLDYIEDMGFNAIWISPI 86


>SPACUNK4.10 |||hydroxyacid dehydrogenase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 334

 Score = 29.9 bits (64), Expect = 0.36
 Identities = 17/56 (30%), Positives = 28/56 (50%)
 Frame = +3

Query: 345 QNLGVAAIRLNYIFQADHYPEDYNNVTSMLDIDRSLGVLKDLQDLIFNIHKRNMSS 512
           + + VAA     I Q  H P+  ++ T+ + I   LG L+     IF +HK N ++
Sbjct: 92  ETVDVAACTARGI-QVSHVPKAVDDATADVGIFLMLGALRGFNQGIFELHKNNWNA 146


>SPAC12B10.13 |||CTLH domain|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 240

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 13/32 (40%), Positives = 19/32 (59%)
 Frame = -2

Query: 677 LNKNPINSLVLETNEL*QQLLDPNS*LQYYLL 582
           +    INS + + NEL  ++LD NS L + LL
Sbjct: 81  IKSGSINSAICKLNELEPEILDTNSELLFELL 112


>SPCC16A11.03c |||DUF2009 protein|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 466

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 16/46 (34%), Positives = 24/46 (52%), Gaps = 1/46 (2%)
 Frame = +1

Query: 19  SKNGDLIIGQAEDVKLNGNLKINNRKL-PSFVNWNWVVIRKILLWV 153
           +KNG L IG     KLN + K     +  S V W+ ++ +  LLW+
Sbjct: 251 TKNGSLKIGYMTGAKLNHDHKTQFFYVDQSLVFWSCMMDQMFLLWL 296


>SPAC823.15 |ppa1||minor serine/threonine protein phosphatase
           Ppa1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 309

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 12/42 (28%), Positives = 21/42 (50%)
 Frame = +3

Query: 306 GDFKGLITKLDYIQNLGVAAIRLNYIFQADHYPEDYNNVTSM 431
           GD  G    L  + N+G  +   NY+F  D+    Y++V ++
Sbjct: 56  GDIHGQFHDLMELFNIGGPSPDTNYLFMGDYVDRGYHSVETV 97


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,859,501
Number of Sequences: 5004
Number of extensions: 59265
Number of successful extensions: 157
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 150
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 154
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 321951680
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -