BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0895 (696 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC13A11.02c |erg11||sterol 14-demethylase|Schizosaccharomyces ... 40 3e-04 SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy... 35 0.013 SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 30 0.28 SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 30 0.36 SPBC17D11.07c |rpn2||19S proteasome regulatory subunit Rpn2|Schi... 30 0.36 SPBC776.09 |ste13||ATP-dependent RNA helicase Ste13|Schizosaccha... 27 2.6 SPCC736.07c |||cell polarity protein |Schizosaccharomyces pombe|... 27 3.4 SPAC3A12.02 |||inorganic pyrophosphatase|Schizosaccharomyces pom... 26 4.5 SPBC1861.03 |mak10||NatC N-acetyltransferase complex subunit Mak... 26 5.9 SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2 |Schiz... 26 5.9 SPAC1952.16 |rga9||RhoGAp, GTPase activator towards Rho/Rac/Cdc4... 25 7.8 >SPAC13A11.02c |erg11||sterol 14-demethylase|Schizosaccharomyces pombe|chr 1|||Manual Length = 495 Score = 39.9 bits (89), Expect = 3e-04 Identities = 23/79 (29%), Positives = 41/79 (51%) Frame = +3 Query: 240 GYSSRIQDKVIEELDQIFGDSDRPVTFQDTLEMKYLERCLMETLRLYPPVPIIARQVNQE 419 G I + + EE ++ G++ + F +M L + ETLRL+PP+ R+V ++ Sbjct: 305 GSKPEIIEMLWEEQKRVVGEN-LELKFDQYKDMPLLNYVIQETLRLHPPIHSHMRKVKRD 363 Query: 420 ITLPSNGKKIPAGTTLVIA 476 + +P + IPA L+ A Sbjct: 364 LPVPGSKIVIPANNYLLAA 382 >SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 541 Score = 34.7 bits (76), Expect = 0.013 Identities = 31/127 (24%), Positives = 51/127 (40%), Gaps = 1/127 (0%) Frame = +3 Query: 255 IQDKVIEELDQIF-GDSDRPVTFQDTLEMKYLERCLMETLRLYPPVPIIARQVNQEITLP 431 + KV EE +I GD D P++ +M Y + E LRL PPV ++ +V + + Sbjct: 341 VLQKVREEQLRIRKGDIDVPLSLDLMEKMTYTRAVVKECLRLRPPVLMVPYRVKKAFPIT 400 Query: 432 SNGKKIPAGTTLVIATYKLHRRPDVYQIQTNLTLTTSFRNVPPTDIIMRSCLFQLGPRSC 611 + +P ++ Y VY N +F GP C Sbjct: 401 PD-YTVPKDAMVIPTLYGALHDSKVYPEPETFNPDRWAPNGLAEQSPKNWMVFGNGPHVC 459 Query: 612 VGRKYAM 632 +G++YA+ Sbjct: 460 LGQRYAV 466 >SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 30.3 bits (65), Expect = 0.28 Identities = 18/71 (25%), Positives = 34/71 (47%), Gaps = 2/71 (2%) Frame = +3 Query: 414 QEITLPSNGKKIPAGTTLVIATYKLHRRPDVYQIQTNLTLTTSFRNVPPTDI--IMRSCL 587 +++ +N +K+ AG + + RR + + + +FRN+P +I + R L Sbjct: 178 RDVVKVANSRKLRAGKGKLRNRRHVQRRGPLVVFNEDAGIVKAFRNIPGVEIVNVRRLNL 237 Query: 588 FQLGPRSCVGR 620 QL P +GR Sbjct: 238 LQLAPGGHLGR 248 >SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 29.9 bits (64), Expect = 0.36 Identities = 18/71 (25%), Positives = 34/71 (47%), Gaps = 2/71 (2%) Frame = +3 Query: 414 QEITLPSNGKKIPAGTTLVIATYKLHRRPDVYQIQTNLTLTTSFRNVPPTDI--IMRSCL 587 +++ +N +K+ AG + + RR + + + +FRN+P +I + R L Sbjct: 178 RDVIKVANSRKLRAGKGKLRNRRHVQRRGPLVVFNEDTGIVKAFRNIPGVEIVNVRRLNL 237 Query: 588 FQLGPRSCVGR 620 QL P +GR Sbjct: 238 LQLAPGGHLGR 248 >SPBC17D11.07c |rpn2||19S proteasome regulatory subunit Rpn2|Schizosaccharomyces pombe|chr 2|||Manual Length = 965 Score = 29.9 bits (64), Expect = 0.36 Identities = 19/46 (41%), Positives = 26/46 (56%), Gaps = 2/46 (4%) Frame = +1 Query: 112 LLLESSQSGVAISDEEIKEQVDTIMFEGH--XXTAAGSSFFLSMMG 243 LLL +G+A DE + E V TI+F + +AAG S L M+G Sbjct: 460 LLLGIGLTGMASRDETLYESVKTILFNDNAVAGSAAGISMGLIMLG 505 >SPBC776.09 |ste13||ATP-dependent RNA helicase Ste13|Schizosaccharomyces pombe|chr 2|||Manual Length = 485 Score = 27.1 bits (57), Expect = 2.6 Identities = 10/32 (31%), Positives = 17/32 (53%) Frame = -2 Query: 602 WSQLKKARTHNNVCWRNVPEGSCQGQICLDLV 507 +S K ++H N + N G C+ +C DL+ Sbjct: 310 YSHAKMLQSHRNRVFHNFRNGVCRNLVCSDLL 341 >SPCC736.07c |||cell polarity protein |Schizosaccharomyces pombe|chr 3|||Manual Length = 699 Score = 26.6 bits (56), Expect = 3.4 Identities = 12/38 (31%), Positives = 21/38 (55%) Frame = +3 Query: 270 IEELDQIFGDSDRPVTFQDTLEMKYLERCLMETLRLYP 383 I E +++ + R V F DTLE+K++ R + + P Sbjct: 472 IVEQEELSSKNPRKVHFSDTLEIKHVSRSGKANIEVIP 509 >SPAC3A12.02 |||inorganic pyrophosphatase|Schizosaccharomyces pombe|chr 1|||Manual Length = 286 Score = 26.2 bits (55), Expect = 4.5 Identities = 16/53 (30%), Positives = 27/53 (50%), Gaps = 1/53 (1%) Frame = +2 Query: 17 WKDF-LSANHPVSRMT*TLTMMSDKRNVWPSLICCWRVHKVVWPFPMRKSKNK 172 WK + N P +++ L +SD +N+ P L+ C R ++ P K KN+ Sbjct: 160 WKILAIDINDPRAKL---LNDISDVQNLMPRLLPCTRDWFAIYKIPDGKPKNR 209 >SPBC1861.03 |mak10||NatC N-acetyltransferase complex subunit Mak10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 708 Score = 25.8 bits (54), Expect = 5.9 Identities = 9/21 (42%), Positives = 13/21 (61%) Frame = +3 Query: 507 YQIQTNLTLTTSFRNVPPTDI 569 +++Q L T RN+PPT I Sbjct: 293 FRVQAQLVATAPVRNIPPTGI 313 >SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 665 Score = 25.8 bits (54), Expect = 5.9 Identities = 10/29 (34%), Positives = 14/29 (48%) Frame = -2 Query: 617 SNATSWSQLKKARTHNNVCWRNVPEGSCQ 531 SN+T K+ HNN C N +C+ Sbjct: 3 SNSTFHEHHAKSHFHNNACQSNASSSACR 31 >SPAC1952.16 |rga9||RhoGAp, GTPase activator towards Rho/Rac/Cdc42-like small GTPases|Schizosaccharomyces pombe|chr 1|||Manual Length = 684 Score = 25.4 bits (53), Expect = 7.8 Identities = 9/31 (29%), Positives = 18/31 (58%) Frame = +3 Query: 225 LPINDGYSSRIQDKVIEELDQIFGDSDRPVT 317 +P+ D + + + +I E + +F D +RP T Sbjct: 614 IPVEDTHPTLLVIDLINEFENLFADLERPST 644 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,147,039 Number of Sequences: 5004 Number of extensions: 71232 Number of successful extensions: 187 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 179 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 185 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 321151040 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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