BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0886 (681 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAPB1A10.04c |cwp1||geranylgeranyltransferase I alpha subunit C... 62 7e-11 SPCC1620.05 |||Rab geranylgeranyltransferase |Schizosaccharomyce... 33 0.050 SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 29 0.62 SPBC359.05 |abc3||ABC transporter Abc3|Schizosaccharomyces pombe... 27 1.9 SPAC1786.01c ||SPAC31G5.20c|triacylglycerol lipase|Schizosacchar... 26 4.4 >SPAPB1A10.04c |cwp1||geranylgeranyltransferase I alpha subunit Cwp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 294 Score = 62.1 bits (144), Expect = 7e-11 Identities = 25/57 (43%), Positives = 33/57 (57%) Frame = +1 Query: 85 ELELTGDALLQDPKNYHAWQHRQWAIKTFGLYEKELDFVDNLITDDVRNNSAWNQRY 255 ELE T D KNYH W +R W ++ F Y +EL + L+ D+ NNSAWN R+ Sbjct: 130 ELEFTKKMFEIDSKNYHVWSYRVWILQNFNDYSQELKLTNELLEKDIYNNSAWNHRF 186 Score = 41.5 bits (93), Expect = 1e-04 Identities = 19/56 (33%), Positives = 26/56 (46%) Frame = +1 Query: 85 ELELTGDALLQDPKNYHAWQHRQWAIKTFGLYEKELDFVDNLITDDVRNNSAWNQR 252 ELE + KNY W HRQ + YE+EL+F + D +N W+ R Sbjct: 96 ELEWLDEIAEDFQKNYQVWHHRQKILSLTKNYERELEFTKKMFEIDSKNYHVWSYR 151 Score = 38.3 bits (85), Expect = 0.001 Identities = 21/51 (41%), Positives = 31/51 (60%) Frame = +3 Query: 252 IFVVNNNLGWSDLICQQEVCYTLEKINFVKNNESAWNYLRGLLIHDKRGLS 404 +F + + WS ++E+ Y +KI F +N+SAWNYL G+L DK G S Sbjct: 189 LFETSKVVSWS---LEEELNYLKDKILFAPDNQSAWNYLCGVL--DKSGPS 234 Score = 35.9 bits (79), Expect = 0.005 Identities = 17/56 (30%), Positives = 29/56 (51%), Gaps = 1/56 (1%) Frame = +1 Query: 88 LELTGDALLQDPKNYHAWQHR-QWAIKTFGLYEKELDFVDNLITDDVRNNSAWNQR 252 L LTG ++ +P +Y W +R Q T + EL+++D + D +N W+ R Sbjct: 62 LNLTGFLIMNNPAHYTVWAYRFQILNHTPSYIDNELEWLDEIAEDFQKNYQVWHHR 117 Score = 35.1 bits (77), Expect = 0.009 Identities = 22/69 (31%), Positives = 38/69 (55%), Gaps = 5/69 (7%) Frame = +1 Query: 55 LVEWLQDPTMELELTGDALLQDPKNYHAWQHRQWAI-KTFGL----YEKELDFVDNLITD 219 +++ D + EL+LT + L +D N AW HR + + +T + E+EL+++ + I Sbjct: 154 ILQNFNDYSQELKLTNELLEKDIYNNSAWNHRFYVLFETSKVVSWSLEEELNYLKDKILF 213 Query: 220 DVRNNSAWN 246 N SAWN Sbjct: 214 APDNQSAWN 222 Score = 28.3 bits (60), Expect = 1.1 Identities = 9/10 (90%), Positives = 10/10 (100%) Frame = +2 Query: 23 KNYQVWHHRR 52 KNYQVWHHR+ Sbjct: 109 KNYQVWHHRQ 118 >SPCC1620.05 |||Rab geranylgeranyltransferase |Schizosaccharomyces pombe|chr 3|||Manual Length = 344 Score = 32.7 bits (71), Expect = 0.050 Identities = 16/62 (25%), Positives = 31/62 (50%), Gaps = 6/62 (9%) Frame = +1 Query: 85 ELELTGDALLQDPKNYHAWQHRQWAIKTF------GLYEKELDFVDNLITDDVRNNSAWN 246 E+ +T L D +N+H W +R++ + L +KE+++ + I + N SA + Sbjct: 129 EMMITEKLLSADARNFHGWHYRRYVVSQIERAGNCSLAKKEMEYTTSAIATNFSNFSALH 188 Query: 247 QR 252 R Sbjct: 189 NR 190 Score = 31.1 bits (67), Expect = 0.15 Identities = 13/46 (28%), Positives = 24/46 (52%), Gaps = 2/46 (4%) Frame = +1 Query: 121 PKNYHAWQHRQWAIKT--FGLYEKELDFVDNLITDDVRNNSAWNQR 252 PK Y + HR+W ++ + + E+ + L++ D RN W+ R Sbjct: 105 PKVYWIFNHRRWCLENAPYPNWNYEMMITEKLLSADARNFHGWHYR 150 Score = 25.8 bits (54), Expect = 5.8 Identities = 9/23 (39%), Positives = 13/23 (56%) Frame = +2 Query: 20 PKNYQVWHHRRFW*NGCKIPQWN 88 PK Y +++HRR+ P WN Sbjct: 105 PKVYWIFNHRRWCLENAPYPNWN 127 >SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharomyces pombe|chr 2|||Manual Length = 1016 Score = 29.1 bits (62), Expect = 0.62 Identities = 14/44 (31%), Positives = 20/44 (45%), Gaps = 1/44 (2%) Frame = +2 Query: 470 VSLHHRCMRGCY*KKDTNCF-HNADRACGVMSGVGH*IRHKLES 598 + H+ C + CY K T C ++D A + H I H ES Sbjct: 435 IDCHYTCHKKCYPKVVTKCISKSSDSASSEYEKINHRIPHHFES 478 >SPBC359.05 |abc3||ABC transporter Abc3|Schizosaccharomyces pombe|chr 2|||Manual Length = 1465 Score = 27.5 bits (58), Expect = 1.9 Identities = 18/69 (26%), Positives = 30/69 (43%) Frame = +3 Query: 243 ESAIFVVNNNLGWSDLICQQEVCYTLEKINFVKNNESAWNYLRGLLIHDKRGLSGNAVIT 422 E + + + WS +Q+V TL +INFV N I K G ++++ Sbjct: 572 EICLEIKSGTFSWSKKTLKQQVTPTLRQINFVAKNGEL------TCIFGKVGAGKSSLLE 625 Query: 423 SFCEELYKN 449 + +YKN Sbjct: 626 ACMGNMYKN 634 >SPAC1786.01c ||SPAC31G5.20c|triacylglycerol lipase|Schizosaccharomyces pombe|chr 1|||Manual Length = 630 Score = 26.2 bits (55), Expect = 4.4 Identities = 14/30 (46%), Positives = 18/30 (60%), Gaps = 1/30 (3%) Frame = +1 Query: 109 LLQDPKNYHAWQHRQWAI-KTFGLYEKELD 195 LLQ+ K Y W+ R A+ K FG E +LD Sbjct: 119 LLQEAKTYEEWKERARALDKYFGNDEWKLD 148 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,018,254 Number of Sequences: 5004 Number of extensions: 64368 Number of successful extensions: 168 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 155 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 168 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 313902888 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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