BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0886 (681 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g59380.1 68416.m06622 farnesyltransferase alpha subunit, puta... 75 3e-14 At4g24490.1 68417.m03510 geranylgeranyl transferase alpha subuni... 42 3e-04 At1g10095.1 68414.m01138 protein prenyltransferase alpha subunit... 36 0.033 At5g41820.1 68418.m05092 geranylgeranyl transferase alpha subuni... 35 0.057 At4g25870.1 68417.m03720 expressed protein contains Pfam profile... 31 0.93 At1g52315.1 68414.m05903 expressed protein 29 3.8 At5g15550.2 68418.m01821 transducin family protein / WD-40 repea... 28 5.0 At5g15550.1 68418.m01820 transducin family protein / WD-40 repea... 28 5.0 At5g01240.2 68418.m00032 amino acid permease, putative strong si... 27 8.7 At5g01240.1 68418.m00031 amino acid permease, putative strong si... 27 8.7 At3g55510.1 68416.m06164 expressed protein 27 8.7 >At3g59380.1 68416.m06622 farnesyltransferase alpha subunit, putative / FTA, putative / protein farnesyltransferase, putative similar to farnesyltransferase alpha subunit [GI:2246442][Pisum sativum] Length = 326 Score = 75.4 bits (177), Expect = 3e-14 Identities = 32/57 (56%), Positives = 38/57 (66%) Frame = +1 Query: 85 ELELTGDALLQDPKNYHAWQHRQWAIKTFGLYEKELDFVDNLITDDVRNNSAWNQRY 255 ELE T L D K+YHAW HRQW ++ G +E ELD+ L+ DV NNSAWNQRY Sbjct: 130 ELEFTRRVLSLDAKHYHAWSHRQWTLRALGGWEDELDYCHELLEADVFNNSAWNQRY 186 Score = 44.0 bits (99), Expect = 9e-05 Identities = 24/80 (30%), Positives = 41/80 (51%), Gaps = 1/80 (1%) Frame = +3 Query: 273 LGWSDLICQQEVCYTLEKINFVKNNESAWNYLRGLLIHDKRGLSGNAVITSFC-EELYKN 449 LG + + + EV YT++ I NES+W YL+ L DK + ++S C L + Sbjct: 195 LGGLEAMRESEVSYTIKAILTNPANESSWRYLKALYKDDKESWISDPSVSSVCLNVLSRT 254 Query: 450 KCRSPFLLAFIIDVCEDAIK 509 C F L+ ++D+ D ++ Sbjct: 255 DCFHGFALSTLLDLLCDGLR 274 Score = 40.3 bits (90), Expect = 0.001 Identities = 18/58 (31%), Positives = 30/58 (51%), Gaps = 2/58 (3%) Frame = +1 Query: 85 ELELTGDALLQDPKNYHAWQHRQWAIKTFG--LYEKELDFVDNLITDDVRNNSAWNQR 252 ELE + KNY W HR+W + G + +EL+F +++ D ++ AW+ R Sbjct: 94 ELEFIERIAEDNSKNYQLWHHRRWVAEKLGPDVAGRELEFTRRVLSLDAKHYHAWSHR 151 Score = 37.1 bits (82), Expect = 0.011 Identities = 18/57 (31%), Positives = 31/57 (54%), Gaps = 2/57 (3%) Frame = +1 Query: 88 LELTGDALLQDPKNYHAWQHRQWAIKTFG--LYEKELDFVDNLITDDVRNNSAWNQR 252 L LT + LL + NY W R+ ++ L+E EL+F++ + D+ +N W+ R Sbjct: 60 LRLTEETLLLNSGNYTVWHFRRLVLEALNHDLFE-ELEFIERIAEDNSKNYQLWHHR 115 Score = 29.9 bits (64), Expect = 1.6 Identities = 9/15 (60%), Positives = 14/15 (93%) Frame = +2 Query: 11 KNSPKNYQVWHHRRF 55 +++ KNYQ+WHHRR+ Sbjct: 103 EDNSKNYQLWHHRRW 117 >At4g24490.1 68417.m03510 geranylgeranyl transferase alpha subunit-related / RAB geranylgeranyltransferase alpha subunit-related low similarity to SP|Q08602 [Rattus norvegicus] Length = 683 Score = 42.3 bits (95), Expect = 3e-04 Identities = 19/57 (33%), Positives = 33/57 (57%), Gaps = 1/57 (1%) Frame = +1 Query: 85 ELELTGDALLQDPKNYHAWQHRQWAIKTFGLYEK-ELDFVDNLITDDVRNNSAWNQR 252 EL L D +N+HAW +R++ ++ E+ EL + D++I ++ N SAW+ R Sbjct: 125 ELRLLEKFQKLDSRNFHAWNYRRFVVELTNRSEQDELQYTDDMINNNFSNYSAWHNR 181 Score = 41.5 bits (93), Expect = 5e-04 Identities = 22/57 (38%), Positives = 29/57 (50%), Gaps = 1/57 (1%) Frame = +1 Query: 85 ELELTGDALLQDPKNYHAWQHRQWAI-KTFGLYEKELDFVDNLITDDVRNNSAWNQR 252 EL + AL Q+ K+Y AW HR+W + K EL ++ D RN AWN R Sbjct: 90 ELRVVESALRQNFKSYGAWHHRKWVLSKGHSSVGNELRLLEKFQKLDSRNFHAWNYR 146 >At1g10095.1 68414.m01138 protein prenyltransferase alpha subunit-related contains weak hit to Pfam profile PF01239: Protein prenyltransferase alpha subunit repeat Length = 431 Score = 35.5 bits (78), Expect = 0.033 Identities = 21/66 (31%), Positives = 29/66 (43%), Gaps = 8/66 (12%) Frame = +1 Query: 79 TMELELTGDALLQDPKNYHAWQHRQWAIKTFG--------LYEKELDFVDNLITDDVRNN 234 T EL L+G L PK+ W HR+W IK + KE + V+++ N Sbjct: 155 TEELRLSGLILSNSPKSESTWSHRRWIIKMISQSFSTLQEIITKESELVESIGERSKMNY 214 Query: 235 SAWNQR 252 AW R Sbjct: 215 RAWYHR 220 >At5g41820.1 68418.m05092 geranylgeranyl transferase alpha subunit-related / RAB geranylgeranyltransferase alpha subunit-related low similarity to SP|Q08602 [Rattus norvegicus] Length = 687 Score = 34.7 bits (76), Expect = 0.057 Identities = 16/45 (35%), Positives = 25/45 (55%) Frame = +1 Query: 85 ELELTGDALLQDPKNYHAWQHRQWAIKTFGLYEKELDFVDNLITD 219 EL + +AL ++ K+Y AW HR+W + G Y L+ L+ D Sbjct: 87 ELGVVQNALERNVKSYGAWYHRKWVLSKKGHYYPSLENELQLLND 131 Score = 32.3 bits (70), Expect = 0.31 Identities = 18/53 (33%), Positives = 30/53 (56%), Gaps = 3/53 (5%) Frame = +1 Query: 103 DALLQDP-KNYHAWQHRQWAIK-TFGLYEKELDFVDNLITD-DVRNNSAWNQR 252 D DP +N+HAW +R++ ++ T E EL + ++I+D SAW+ R Sbjct: 142 DEKQDDPSRNFHAWNYRRFVVELTKTSEEDELQYTTDMISDISFTIYSAWHYR 194 >At4g25870.1 68417.m03720 expressed protein contains Pfam profile PF03267: Arabidopsis protein of unknown function, DUF266 Length = 389 Score = 30.7 bits (66), Expect = 0.93 Identities = 11/29 (37%), Positives = 19/29 (65%) Frame = +1 Query: 139 WQHRQWAIKTFGLYEKELDFVDNLITDDV 225 W R+W KT+G E L+F+ N+ ++D+ Sbjct: 307 WSERRWHPKTYGGNEISLEFMKNVTSEDM 335 >At1g52315.1 68414.m05903 expressed protein Length = 347 Score = 28.7 bits (61), Expect = 3.8 Identities = 15/47 (31%), Positives = 27/47 (57%), Gaps = 1/47 (2%) Frame = +3 Query: 312 YTLEKINFVKNNESAWNYLRG-LLIHDKRGLSGNAVITSFCEELYKN 449 Y + +NF+KN + + Y R LL+ + R +S +I FC+ + +N Sbjct: 39 YKTDIVNFLKNGQDSEAYRRAELLLEELRIISCYDLIERFCDCISEN 85 >At5g15550.2 68418.m01821 transducin family protein / WD-40 repeat family protein similar to YTM1 - Homo sapiens, EMBL:AF242546; contains Pfam PF00400: WD domain, G-beta repeat (7 copies,1 weak); Length = 402 Score = 28.3 bits (60), Expect = 5.0 Identities = 13/25 (52%), Positives = 16/25 (64%) Frame = -2 Query: 86 SIVGSCSHSTKTYGGAIPDNSLVNS 12 S GSCSH + + GAI +LVNS Sbjct: 137 SSAGSCSHILEGHSGAISSVALVNS 161 >At5g15550.1 68418.m01820 transducin family protein / WD-40 repeat family protein similar to YTM1 - Homo sapiens, EMBL:AF242546; contains Pfam PF00400: WD domain, G-beta repeat (7 copies,1 weak); Length = 433 Score = 28.3 bits (60), Expect = 5.0 Identities = 13/25 (52%), Positives = 16/25 (64%) Frame = -2 Query: 86 SIVGSCSHSTKTYGGAIPDNSLVNS 12 S GSCSH + + GAI +LVNS Sbjct: 137 SSAGSCSHILEGHSGAISSVALVNS 161 >At5g01240.2 68418.m00032 amino acid permease, putative strong similarity to AUX1 GI:1531758 from [Arabidopsis thaliana]; contains Pfam profile PF01490: Transmembrane amino acid transporter protein Length = 408 Score = 27.5 bits (58), Expect = 8.7 Identities = 13/27 (48%), Positives = 16/27 (59%) Frame = -3 Query: 136 HDSSLDLVEVHRLSIQVPLWDLAAILP 56 H SL L + RL + VP+W LA I P Sbjct: 271 HTKSLCLRALVRLPVVVPIWFLAIIFP 297 >At5g01240.1 68418.m00031 amino acid permease, putative strong similarity to AUX1 GI:1531758 from [Arabidopsis thaliana]; contains Pfam profile PF01490: Transmembrane amino acid transporter protein Length = 488 Score = 27.5 bits (58), Expect = 8.7 Identities = 13/27 (48%), Positives = 16/27 (59%) Frame = -3 Query: 136 HDSSLDLVEVHRLSIQVPLWDLAAILP 56 H SL L + RL + VP+W LA I P Sbjct: 351 HTKSLCLRALVRLPVVVPIWFLAIIFP 377 >At3g55510.1 68416.m06164 expressed protein Length = 594 Score = 27.5 bits (58), Expect = 8.7 Identities = 18/65 (27%), Positives = 34/65 (52%), Gaps = 2/65 (3%) Frame = +3 Query: 429 CEELYKNKCRSPFLLAFIIDVCEDAIKKKIQTASTMQTEPVELCQ-ALATR-YDTN*KVN 602 C+ N + PFL ++++C ++K AS T+ +L + ALAT+ + K++ Sbjct: 305 CDIPKANSEQRPFLRDSLVELCSQDVQKSYTKASVSITQLAKLLKMALATKNKEAVEKIH 364 Query: 603 IGIYL 617 G Y+ Sbjct: 365 SGEYI 369 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,515,771 Number of Sequences: 28952 Number of extensions: 326258 Number of successful extensions: 751 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 712 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 749 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1438152744 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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