BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0873 (664 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces p... 29 0.79 SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 27 2.4 SPCC1450.02 ||SPCC191.13|bromodomain protein|Schizosaccharomyces... 27 3.2 SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosacchar... 26 4.2 SPCC970.09 |sec8||exocyst complex subunit Sec8|Schizosaccharomyc... 26 5.6 SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo... 26 5.6 SPBC19C2.05 |ran1|pat1|serine/threonine protein kinase Ran1|Schi... 25 7.4 SPAC1952.03 |||cysteine protease, OTU family|Schizosaccharomyces... 25 7.4 SPCC584.05 |sec1||SNARE binding protein Sec1|Schizosaccharomyces... 25 7.4 SPCC1442.05c |||conserved fungal protein|Schizosaccharomyces pom... 25 7.4 SPCC1183.01 |sec15|SPCC1672.13|exocyst complex subunit Sec15 |Sc... 25 7.4 SPAC18G6.07c |mra1||ribosome biogenesis protein Mra1|Schizosacch... 25 7.4 SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyc... 25 9.7 >SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1040 Score = 28.7 bits (61), Expect = 0.79 Identities = 15/34 (44%), Positives = 21/34 (61%) Frame = +2 Query: 272 IARDFLSGKMGVDKFLEDFEPIRKQMHIRKYKAE 373 IA +F KM DK +ED PI+K H+ ++AE Sbjct: 500 IAYEF-KNKMAYDKSIEDNAPIKKFEHLPFFEAE 532 >SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 547 Score = 27.1 bits (57), Expect = 2.4 Identities = 13/34 (38%), Positives = 18/34 (52%) Frame = +3 Query: 96 ELERMKAELQEKSELGEQLCTRIQELLDDYKTKS 197 +LE +K +E+ + E L RIQ L TKS Sbjct: 435 QLEHLKHNYEEEKSMNENLLVRIQTLEKQNTTKS 468 >SPCC1450.02 ||SPCC191.13|bromodomain protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 578 Score = 26.6 bits (56), Expect = 3.2 Identities = 15/53 (28%), Positives = 31/53 (58%) Frame = +3 Query: 102 ERMKAELQEKSELGEQLCTRIQELLDDYKTKSAGISPDTTHALLQTAAAESKN 260 E+ K + ++E E++ R+Q+ LD + K++ SP++ +A + +ES N Sbjct: 520 EKKKGRVLSETEQAEKI-RRLQQQLDRFAGKTSPTSPESNNA-ANVSDSESDN 570 >SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1275 Score = 26.2 bits (55), Expect = 4.2 Identities = 10/17 (58%), Positives = 13/17 (76%) Frame = -1 Query: 385 SDIFLGFVLPYMHLFTD 335 SDIF+ +L Y H+FTD Sbjct: 1232 SDIFMMLILNYDHIFTD 1248 >SPCC970.09 |sec8||exocyst complex subunit Sec8|Schizosaccharomyces pombe|chr 3|||Manual Length = 1088 Score = 25.8 bits (54), Expect = 5.6 Identities = 10/22 (45%), Positives = 15/22 (68%) Frame = -1 Query: 391 NNSDIFLGFVLPYMHLFTDGFK 326 NNSD+ G+V + L+TD +K Sbjct: 852 NNSDLMKGYVTVCIELYTDFYK 873 >SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 564 Score = 25.8 bits (54), Expect = 5.6 Identities = 11/30 (36%), Positives = 17/30 (56%) Frame = +1 Query: 403 SECHMVWCTKPYLPIFQLFTITESHKVCPT 492 S+ +W K YL + Q++T +HKV T Sbjct: 47 SDKRQIWLEKYYLFVRQIYTYLVTHKVILT 76 >SPBC19C2.05 |ran1|pat1|serine/threonine protein kinase Ran1|Schizosaccharomyces pombe|chr 2|||Manual Length = 470 Score = 25.4 bits (53), Expect = 7.4 Identities = 10/27 (37%), Positives = 17/27 (62%) Frame = +2 Query: 392 EKQHQNAIWYGVQNHTYPYSNYLPSQN 472 ++Q Q + G+Q YP+ Y+P+QN Sbjct: 322 QQQQQMNLQQGIQG--YPHQGYMPTQN 346 >SPAC1952.03 |||cysteine protease, OTU family|Schizosaccharomyces pombe|chr 1|||Manual Length = 324 Score = 25.4 bits (53), Expect = 7.4 Identities = 13/40 (32%), Positives = 24/40 (60%) Frame = +3 Query: 87 KEPELERMKAELQEKSELGEQLCTRIQELLDDYKTKSAGI 206 ++ LER KAE+++ SE E ++ +L ++ K K + I Sbjct: 140 QKERLERRKAEMKKMSEQAELESEKMADLKNEEKKKFSKI 179 >SPCC584.05 |sec1||SNARE binding protein Sec1|Schizosaccharomyces pombe|chr 3|||Manual Length = 693 Score = 25.4 bits (53), Expect = 7.4 Identities = 12/33 (36%), Positives = 18/33 (54%) Frame = +2 Query: 317 LEDFEPIRKQMHIRKYKAEKNVRIVEKQHQNAI 415 L DF+ +R Q + A++ + I EKQ N I Sbjct: 335 LSDFQELRDQYSLHLTMAQECMNIFEKQQLNLI 367 >SPCC1442.05c |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 177 Score = 25.4 bits (53), Expect = 7.4 Identities = 15/45 (33%), Positives = 25/45 (55%), Gaps = 1/45 (2%) Frame = +2 Query: 305 VDKFLED-FEPIRKQMHIRKYKAEKNVRIVEKQHQNAIWYGVQNH 436 + F+E F IRKQ + + +KN++ +K +A+ GVQ H Sbjct: 123 IGAFVEKRFPAIRKQRMLVLEQTQKNIQNAQKSMTSAV-EGVQKH 166 >SPCC1183.01 |sec15|SPCC1672.13|exocyst complex subunit Sec15 |Schizosaccharomyces pombe|chr 3|||Manual Length = 785 Score = 25.4 bits (53), Expect = 7.4 Identities = 16/84 (19%), Positives = 39/84 (46%), Gaps = 1/84 (1%) Frame = +2 Query: 221 SCTSANSSSRIKEQSDNIARDFLSGKMGVDKFLEDFEPIRKQMHIRKYKAEKNVRIVEKQ 400 S +A+S +++ ++ + D + + DKF+ DF ++ + I+ + ++ Sbjct: 689 SIVNADSFIELRQCANLLLGDNMEEYLDTDKFMRDFNRLQPAVAIKFLERYNIPACLQDN 748 Query: 401 HQNAIWYGVQNHT-YPYSNYLPSQ 469 N + + N++ P SN P + Sbjct: 749 FTNLLCRHINNYSANPLSNDRPKR 772 >SPAC18G6.07c |mra1||ribosome biogenesis protein Mra1|Schizosaccharomyces pombe|chr 1|||Manual Length = 359 Score = 25.4 bits (53), Expect = 7.4 Identities = 21/63 (33%), Positives = 24/63 (38%) Frame = +3 Query: 153 CTRIQELLDDYKTKSAGISPDTTHALLQTAAAESKNSQITLQETFYLEKWVLINF*KILN 332 C Q +L A PD TH L T N LQ + K VLI +N Sbjct: 177 CDDHQGILKKLNRNIAQARPDITHQCLLTLLDSPLNKAGRLQVYIHTAKKVLIE----VN 232 Query: 333 PSV 341 PSV Sbjct: 233 PSV 235 >SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyces pombe|chr 1|||Manual Length = 1085 Score = 25.0 bits (52), Expect = 9.7 Identities = 15/61 (24%), Positives = 26/61 (42%) Frame = +3 Query: 87 KEPELERMKAELQEKSELGEQLCTRIQELLDDYKTKSAGISPDTTHALLQTAAAESKNSQ 266 KE + K + Q+ +L E L L+D KT + T H L T + + ++ Sbjct: 922 KEHSEDNTKEKHQQLLDLLESLVGNNDNLIDSIKTPHTELQKITDHVLKGTTSLANHTNE 981 Query: 267 I 269 + Sbjct: 982 L 982 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,712,950 Number of Sequences: 5004 Number of extensions: 56969 Number of successful extensions: 187 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 183 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 187 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 301829700 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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