BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0859 (686 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP1E11.08 |||ribosome biogenesis protein Nsa2 |Schizosaccharom... 130 1e-31 SPAC23C4.14 |alg1||mannosyltransferase complex subunit Alg1 |Sch... 28 1.1 SPAC1002.08c |mtf1||mitochondrial RNA polymerase specificity fac... 27 3.4 SPBC16A3.16 |||mitochondrial inner membrane protein involved in ... 26 5.9 SPBC28E12.05 |esf2|SPBC3H7.17c|U3 snoRNP-associated protein Esf2... 26 5.9 SPAC22A12.09c |sap114||splicing factor Sap114|Schizosaccharomyce... 25 7.8 >SPCP1E11.08 |||ribosome biogenesis protein Nsa2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 260 Score = 130 bits (315), Expect = 1e-31 Identities = 63/111 (56%), Positives = 77/111 (69%) Frame = +1 Query: 175 KTKA*RESWKMGCPHP*SESSSGRRSIKVLKSGKSKRKAWXRMVTKVTFVGEXFTRKPPK 354 K K ++ K P P + KV+++GKSK+ +W RM+TK TFVG+ FTR+P K Sbjct: 107 KQKRKEKAAKYSVPLPQVRGVAEEEMFKVIRTGKSKKNSWKRMITKATFVGDGFTRRPVK 166 Query: 355 FERFIRPMALRFKKAHVTHPELKATFCLPIIGVKKNPSSQMYTSLGVITKG 507 +ERFIRPMALR KKA+VTH EL T LPIIGVKKNP S YT LGV+TKG Sbjct: 167 YERFIRPMALRQKKANVTHKELGVTMQLPIIGVKKNPQSPTYTQLGVLTKG 217 Score = 80.2 bits (189), Expect = 3e-16 Identities = 35/43 (81%), Positives = 39/43 (90%) Frame = +3 Query: 504 G*VIEVNISELGLVTQAGKVVWGKYAQVTNNPENDGCINAVLL 632 G VIEVN+SELGLVT GKVVWGKYAQ+TNNPE DGC+NA+LL Sbjct: 217 GTVIEVNVSELGLVTSGGKVVWGKYAQITNNPELDGCVNALLL 259 Score = 68.9 bits (161), Expect = 6e-13 Identities = 34/72 (47%), Positives = 50/72 (69%) Frame = +2 Query: 44 EKENQSARREECQTEHREGRXSALPVYLLDRDVQSRAKVLSNMIKQKRKEKAGKWDVPIP 223 E+ N + R + QT+ A+P YLLDR+ +S+AK+LS+ +KQKRKEKA K+ VP+P Sbjct: 69 EERNATQRGSDAQTQ------GAVPTYLLDREQESQAKMLSSAVKQKRKEKAAKYSVPLP 122 Query: 224 KVRAQADAEVSK 259 +VR A+ E+ K Sbjct: 123 QVRGVAEEEMFK 134 Score = 37.9 bits (84), Expect = 0.001 Identities = 17/28 (60%), Positives = 20/28 (71%) Frame = +3 Query: 3 KYSXKERRNEKIQMKKKIKAHEEKNVKQ 86 K ++RR EKIQMKK IK HEE+N Q Sbjct: 48 KLYQEKRRKEKIQMKKTIKQHEERNATQ 75 >SPAC23C4.14 |alg1||mannosyltransferase complex subunit Alg1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 424 Score = 28.3 bits (60), Expect = 1.1 Identities = 13/41 (31%), Positives = 18/41 (43%) Frame = +1 Query: 373 PMALRFKKAHVTHPELKATFCLPIIGVKKNPSSQMYTSLGV 495 PM F + HP K FC+P + ++ P LGV Sbjct: 287 PMKEEFSQYIKKHPLHKVRFCMPWLSIEDYPQVMACADLGV 327 >SPAC1002.08c |mtf1||mitochondrial RNA polymerase specificity factor|Schizosaccharomyces pombe|chr 1|||Manual Length = 366 Score = 26.6 bits (56), Expect = 3.4 Identities = 12/33 (36%), Positives = 20/33 (60%) Frame = +2 Query: 98 GRXSALPVYLLDRDVQSRAKVLSNMIKQKRKEK 196 GR S YL+++++ A V SN++K+ EK Sbjct: 29 GRSSYKSSYLVNQNLMDEALVKSNLLKEYNSEK 61 >SPBC16A3.16 |||mitochondrial inner membrane protein involved in cytochrome c oxidase assembly Pet191 |Schizosaccharomyces pombe|chr 2|||Manual Length = 85 Score = 25.8 bits (54), Expect = 5.9 Identities = 11/31 (35%), Positives = 18/31 (58%) Frame = +3 Query: 18 ERRNEKIQMKKKIKAHEEKNVKQNTEKVAEV 110 E + + + M K+ + EKN Q+TEK + V Sbjct: 52 ECKRQMLDMTKRYRIAPEKNTDQDTEKPSNV 82 >SPBC28E12.05 |esf2|SPBC3H7.17c|U3 snoRNP-associated protein Esf2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 334 Score = 25.8 bits (54), Expect = 5.9 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = -1 Query: 683 EIGSHLDFSPGLNNKLDQQHSINA 612 E H DFS G N+LD++ + NA Sbjct: 77 ETSDHEDFSGGSENELDEETTKNA 100 >SPAC22A12.09c |sap114||splicing factor Sap114|Schizosaccharomyces pombe|chr 1|||Manual Length = 481 Score = 25.4 bits (53), Expect = 7.8 Identities = 14/43 (32%), Positives = 22/43 (51%) Frame = +2 Query: 68 REECQTEHREGRXSALPVYLLDRDVQSRAKVLSNMIKQKRKEK 196 RE + +E R + Y L +Q R + S+M QK+K+K Sbjct: 201 REPISSPEQELRENVRDPYSLLSKIQPRVRWQSHMESQKKKQK 243 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,551,360 Number of Sequences: 5004 Number of extensions: 48960 Number of successful extensions: 151 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 145 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 151 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 317927284 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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