BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0854 (694 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 56 5e-09 SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 44 2e-05 SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 39 8e-04 SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 38 0.001 SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 38 0.002 SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 35 0.010 SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 29 0.48 SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|c... 29 0.84 SPAC1786.03 |cut11|SPAC24C9.01|integral membrane nucleoporin|Sch... 28 1.1 SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2... 27 1.9 SPAC6C3.06c |||P-type ATPase, calcium transporting|Schizosacchar... 27 1.9 SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr... 25 7.9 SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces po... 25 7.9 SPMIT.02 |||mitochondrial DNA binding endonuclease|Schizosacchar... 25 7.9 SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 25 7.9 >SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 103 Score = 56.0 bits (129), Expect = 5e-09 Identities = 23/48 (47%), Positives = 33/48 (68%) Frame = +1 Query: 70 IKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMSDS 213 + DS + K+ + + DKLVV+DF ATWCGPCK I PK ++ + SD+ Sbjct: 5 VSDSSEFKSIVCQ--DKLVVVDFFATWCGPCKAIAPKFEQFSNTYSDA 50 Score = 34.7 bits (76), Expect = 0.013 Identities = 13/28 (46%), Positives = 21/28 (75%) Frame = +3 Query: 255 ASEYNINSMPTFVFVKNGKKLDEFSGAN 338 A+E +++MP+F KNG+K++E GAN Sbjct: 64 AAEAGVHAMPSFFLYKNGEKIEEIVGAN 91 >SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccharomyces pombe|chr 2|||Manual Length = 121 Score = 44.4 bits (100), Expect = 2e-05 Identities = 17/44 (38%), Positives = 29/44 (65%) Frame = +1 Query: 70 IKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAE 201 ++ D TR++ DK+ V+DF A WCGPCK + P L++++ + Sbjct: 22 VESFGDYNTRIS--ADKVTVVDFYADWCGPCKYLKPFLEKLSEQ 63 >SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 359 Score = 38.7 bits (86), Expect = 8e-04 Identities = 16/49 (32%), Positives = 28/49 (57%) Frame = +1 Query: 64 IHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMSD 210 + ++ ++L+ + A K +I+F ATWCG CK + P +E+ A D Sbjct: 23 VELQSLNELENTI-RASKKGALIEFYATWCGHCKSLAPVYEELGALFED 70 Score = 30.3 bits (65), Expect = 0.28 Identities = 10/25 (40%), Positives = 16/25 (64%) Frame = +1 Query: 118 KLVVIDFMATWCGPCKMIGPKLDEI 192 K V+++F A WCG CK + P + + Sbjct: 159 KDVLVEFYADWCGYCKRLAPTYETL 183 >SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 492 Score = 37.9 bits (84), Expect = 0.001 Identities = 14/31 (45%), Positives = 21/31 (67%) Frame = +1 Query: 118 KLVVIDFMATWCGPCKMIGPKLDEIAAEMSD 210 K V+++F A WCG CK + P +++A E SD Sbjct: 374 KDVLVEFYAPWCGHCKNLAPTYEKLAEEYSD 404 Score = 36.3 bits (80), Expect = 0.004 Identities = 12/30 (40%), Positives = 20/30 (66%) Frame = +1 Query: 115 DKLVVIDFMATWCGPCKMIGPKLDEIAAEM 204 DK++++ F A WCG CK + P+ + A E+ Sbjct: 39 DKVLMVKFYAPWCGHCKALAPEYESAADEL 68 Score = 31.1 bits (67), Expect = 0.16 Identities = 12/25 (48%), Positives = 17/25 (68%) Frame = +3 Query: 258 SEYNINSMPTFVFVKNGKKLDEFSG 332 SEY+I PT KNGK++ ++SG Sbjct: 88 SEYSIRGYPTLNVFKNGKQISQYSG 112 >SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosaccharomyces pombe|chr 2|||Manual Length = 290 Score = 37.5 bits (83), Expect = 0.002 Identities = 15/28 (53%), Positives = 21/28 (75%) Frame = +3 Query: 255 ASEYNINSMPTFVFVKNGKKLDEFSGAN 338 AS + +MPTFVF +NGK++D +GAN Sbjct: 67 ASGLGVKAMPTFVFFENGKQIDMLTGAN 94 Score = 34.3 bits (75), Expect = 0.017 Identities = 11/26 (42%), Positives = 17/26 (65%) Frame = +1 Query: 124 VVIDFMATWCGPCKMIGPKLDEIAAE 201 + +D A WCGPCK I P ++A++ Sbjct: 22 LAVDCYADWCGPCKAISPLFSQLASK 47 >SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 35.1 bits (77), Expect = 0.010 Identities = 12/53 (22%), Positives = 28/53 (52%) Frame = +1 Query: 58 MSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMSDSI 216 MS+ I + + L +++++++F A W PCK + D+ A + +++ Sbjct: 1 MSVEITFVEQFQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKDTKNAV 53 Score = 29.1 bits (62), Expect = 0.64 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = +3 Query: 255 ASEYNINSMPTFVFVKNGKKLDEFSGAN 338 A +++N++P FV + K L SGAN Sbjct: 66 AESFDVNAVPLFVLIHGAKVLARISGAN 93 >SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 363 Score = 29.5 bits (63), Expect = 0.48 Identities = 14/43 (32%), Positives = 23/43 (53%) Frame = +1 Query: 76 DSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEM 204 +S + + + G LVV F A WCG CK + P ++A+ + Sbjct: 37 NSKNFRKFVKAKGPSLVV--FYAPWCGYCKKLVPTYQKLASNL 77 >SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1687 Score = 28.7 bits (61), Expect = 0.84 Identities = 11/30 (36%), Positives = 17/30 (56%) Frame = -2 Query: 495 KFITDVKNIFYSFIKNVYMFFFFNSILVSI 406 KFI D +N F+ I Y F F ++L+ + Sbjct: 436 KFILDSQNFFFESINTEYSFIIFTNLLMHL 465 >SPAC1786.03 |cut11|SPAC24C9.01|integral membrane nucleoporin|Schizosaccharomyces pombe|chr 1|||Manual Length = 601 Score = 28.3 bits (60), Expect = 1.1 Identities = 11/32 (34%), Positives = 18/32 (56%) Frame = -3 Query: 515 TQKLISSNSLQTLKTFFIHLLKTYTCFFFLIP 420 T SS +L +F+ L++ + CFFF+ P Sbjct: 45 TSYWFSSGPFISLSFWFLSLVRGFVCFFFMFP 76 >SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2|Schizosaccharomyces pombe|chr 1|||Manual Length = 750 Score = 27.5 bits (58), Expect = 1.9 Identities = 12/40 (30%), Positives = 22/40 (55%) Frame = -2 Query: 516 NTKINIFKFITDVKNIFYSFIKNVYMFFFFNSILVSIGGC 397 +++ N ++TD +NIF +F+ N + F + L I C Sbjct: 72 SSESNSIDYLTDTQNIFPNFVNNENNYQFSTAPLNPIDAC 111 >SPAC6C3.06c |||P-type ATPase, calcium transporting|Schizosaccharomyces pombe|chr 1|||Manual Length = 1033 Score = 27.5 bits (58), Expect = 1.9 Identities = 12/29 (41%), Positives = 18/29 (62%), Gaps = 1/29 (3%) Frame = +2 Query: 560 NNSINKTRY*LIIYNPK-LYFYFGFFYQV 643 NN++ T+Y L + PK LY F +FY + Sbjct: 91 NNAVTNTKYDLFTFLPKCLYEQFRYFYNM 119 >SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr 2|||Manual Length = 905 Score = 25.4 bits (53), Expect = 7.9 Identities = 15/47 (31%), Positives = 25/47 (53%) Frame = +1 Query: 76 DSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMSDSI 216 + D + RL EAG + +A CGPC + + D+IA+ ++I Sbjct: 431 ERDGITERLEEAGATV-----LANACGPCIGMWKRTDDIASGEPNAI 472 >SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces pombe|chr 3|||Manual Length = 2812 Score = 25.4 bits (53), Expect = 7.9 Identities = 12/53 (22%), Positives = 27/53 (50%) Frame = -3 Query: 602 CKLLTNI*FYLLNCLITTEPIFFAFQRLQTQKLISSNSLQTLKTFFIHLLKTY 444 C ++ I Y L+ T+EPIF+ + QK++ + + + + + +K + Sbjct: 907 CFGMSAIGAYFLDVARTSEPIFYKCLEILAQKILMNYDYERDEVYLMIFIKIF 959 >SPMIT.02 |||mitochondrial DNA binding endonuclease|Schizosaccharomyces pombe|chr mitochondrial|||Manual Length = 384 Score = 25.4 bits (53), Expect = 7.9 Identities = 12/55 (21%), Positives = 24/55 (43%) Frame = -2 Query: 540 IFCIPEAANTKINIFKFITDVKNIFYSFIKNVYMFFFFNSILVSIGGCVQL*SLF 376 I+ + T + + + +++ F S ++N+ FF S G C+ LF Sbjct: 27 IYFLTSKKITNLGKIRLVKSIRDSFLSQLENILCFFLVYRTTYSFGVCLMKRFLF 81 >SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1029 Score = 25.4 bits (53), Expect = 7.9 Identities = 9/22 (40%), Positives = 16/22 (72%) Frame = -3 Query: 509 KLISSNSLQTLKTFFIHLLKTY 444 KL+SSN+LQ + F+ ++K + Sbjct: 412 KLVSSNTLQAMSHFYATMIKLF 433 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,689,475 Number of Sequences: 5004 Number of extensions: 51484 Number of successful extensions: 168 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 156 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 168 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 321951680 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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