BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0826 (528 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 76 4e-15 SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 40 3e-04 SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 37 0.002 SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 37 0.002 SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 36 0.003 SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 35 0.007 SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 35 0.007 SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 32 0.061 SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 31 0.080 SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 30 0.19 SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 28 0.99 SPBP35G2.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr... 27 1.3 SPCC736.11 |ago1|csp9|argonaute|Schizosaccharomyces pombe|chr 3|... 26 4.0 SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 25 5.3 SPAC140.01 |sdh2||succinate dehydrogenase |Schizosaccharomyces p... 25 5.3 SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 5.3 SPCC645.03c |isa1||iron-sulfur protein Isa1|Schizosaccharomyces ... 25 5.3 SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 25 7.0 SPBC27B12.11c |||transcription factor |Schizosaccharomyces pombe... 25 9.2 SPBC336.01 |fbh1|fdh1, fdh|DNA helicase I|Schizosaccharomyces po... 25 9.2 >SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosaccharomyces pombe|chr 1|||Manual Length = 227 Score = 75.8 bits (178), Expect = 4e-15 Identities = 32/55 (58%), Positives = 40/55 (72%) Frame = +3 Query: 357 QFKTNTRLCLSITDFHPDTWNPAWSISTILTGLLSFMLEKTPTLGSIETSRYQKR 521 +F+TNTRLCLS +DFHP +WNP+W +STIL GL+SFM T G I TS +R Sbjct: 79 RFQTNTRLCLSFSDFHPKSWNPSWMVSTILVGLVSFMTSDEITTGGIVTSESTRR 133 Score = 58.8 bits (136), Expect = 5e-10 Identities = 22/32 (68%), Positives = 28/32 (87%) Frame = +1 Query: 262 SPYEGGYYHGKIIFPREFPFKPPSIYMITPNG 357 +PYEGG YHG +IFP ++PFKPP+I MITP+G Sbjct: 47 TPYEGGQYHGTLIFPPDYPFKPPAIRMITPSG 78 Score = 52.8 bits (121), Expect = 3e-08 Identities = 22/47 (46%), Positives = 30/47 (63%) Frame = +2 Query: 137 GATSRLKQDYLRLKNDPVPYVTAEPVPSNILEWHYVVKGRRKVPTKG 277 GA RL ++YL L+ +PV V A+P NILEWHY++ G P +G Sbjct: 5 GAYKRLMKEYLALQKNPVELVDAKPATENILEWHYIITGPPDTPYEG 51 >SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 217 Score = 39.5 bits (88), Expect = 3e-04 Identities = 20/51 (39%), Positives = 29/51 (56%), Gaps = 4/51 (7%) Frame = +1 Query: 256 EKSPYEGGYYHGKIIFPREFPFKPPSIYMIT----PNGSSRQTHDCASVLQ 396 E +PYEGGY+ I P ++PF+PP + T PN SS+ C +L+ Sbjct: 44 EGTPYEGGYFVVDIEIPIDYPFRPPKMNFDTKIYHPNVSSQTGAICLDILK 94 >SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosaccharomyces pombe|chr 2|||Manual Length = 167 Score = 36.7 bits (81), Expect = 0.002 Identities = 12/31 (38%), Positives = 20/31 (64%) Frame = +1 Query: 256 EKSPYEGGYYHGKIIFPREFPFKPPSIYMIT 348 E + YEGG++H + FP+++P PP + T Sbjct: 46 EDTLYEGGFFHATLSFPQDYPLMPPKMKFTT 76 >SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccharomyces pombe|chr 2|||Manual Length = 147 Score = 36.7 bits (81), Expect = 0.002 Identities = 14/29 (48%), Positives = 18/29 (62%) Frame = +1 Query: 262 SPYEGGYYHGKIIFPREFPFKPPSIYMIT 348 SPY GG + I FP ++PFKPP + T Sbjct: 43 SPYAGGVFFLSIHFPTDYPFKPPKVNFTT 71 Score = 27.9 bits (59), Expect = 0.99 Identities = 13/39 (33%), Positives = 21/39 (53%) Frame = +3 Query: 366 TNTRLCLSITDFHPDTWNPAWSISTILTGLLSFMLEKTP 482 +N +CL I D W+PA +IS +L + S + + P Sbjct: 80 SNGSICLDILR---DQWSPALTISKVLLSICSLLTDPNP 115 >SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Schizosaccharomyces pombe|chr 1|||Manual Length = 148 Score = 36.3 bits (80), Expect = 0.003 Identities = 12/31 (38%), Positives = 21/31 (67%) Frame = +1 Query: 256 EKSPYEGGYYHGKIIFPREFPFKPPSIYMIT 348 ++S YEGG +H ++ P E+P PP++ +T Sbjct: 42 QQSAYEGGKFHLELFLPDEYPMMPPNVRFLT 72 >SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosaccharomyces pombe|chr 2|||Manual Length = 160 Score = 35.1 bits (77), Expect = 0.007 Identities = 21/76 (27%), Positives = 34/76 (44%) Frame = +3 Query: 240 TSLKGGEKSLRRGILSWKDYFPKGISVQTTFDLHDNTEWQFKTNTRLCLSITDFHPDTWN 419 T +GG+ L + P + QT +H N W TN +C+ I H W+ Sbjct: 47 TPYEGGQWVLDIHVHEGYPISPPSVYFQTKI-VHPNISW---TNGEVCMDILKTH---WS 99 Query: 420 PAWSISTILTGLLSFM 467 PAWS+ + ++S + Sbjct: 100 PAWSLQSACLAIISLL 115 Score = 32.3 bits (70), Expect = 0.046 Identities = 17/51 (33%), Positives = 26/51 (50%), Gaps = 4/51 (7%) Frame = +1 Query: 259 KSPYEGGYYHGKIIFPREFPFKPPSIY----MITPNGSSRQTHDCASVLQT 399 ++PYEGG + I +P PPS+Y ++ PN S C +L+T Sbjct: 46 ETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPNISWTNGEVCMDILKT 96 >SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosaccharomyces pombe|chr 1|||Manual Length = 155 Score = 35.1 bits (77), Expect = 0.007 Identities = 18/49 (36%), Positives = 29/49 (59%), Gaps = 4/49 (8%) Frame = +1 Query: 262 SPYEGGYYHGKIIFPREFPFKPPSIYMIT----PNGSSRQTHDCASVLQ 396 S Y GG +H + FP ++PF+PP+I T PN S + + C ++L+ Sbjct: 49 SVYAGGKFHFSLKFPLDYPFQPPTIEFTTRIYHPNFDS-EGNVCLAILK 96 >SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizosaccharomyces pombe|chr 2|||Manual Length = 166 Score = 31.9 bits (69), Expect = 0.061 Identities = 10/25 (40%), Positives = 17/25 (68%) Frame = +1 Query: 262 SPYEGGYYHGKIIFPREFPFKPPSI 336 +P+EGG Y + FP ++P PP++ Sbjct: 48 TPFEGGLYPATLKFPSDYPLGPPTL 72 >SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 31.5 bits (68), Expect = 0.080 Identities = 12/31 (38%), Positives = 17/31 (54%) Frame = +1 Query: 259 KSPYEGGYYHGKIIFPREFPFKPPSIYMITP 351 K+ +EGG Y + FP E+P +PP P Sbjct: 50 KTSWEGGLYKLTMAFPEEYPTRPPKCRFTPP 80 >SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosaccharomyces pombe|chr 2|||Manual Length = 184 Score = 30.3 bits (65), Expect = 0.19 Identities = 11/26 (42%), Positives = 17/26 (65%) Frame = +1 Query: 259 KSPYEGGYYHGKIIFPREFPFKPPSI 336 ++PY GG + + P E+P+K PSI Sbjct: 41 ETPYSGGIWKVHVELPSEYPWKSPSI 66 >SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C |Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 27.9 bits (59), Expect = 0.99 Identities = 10/28 (35%), Positives = 16/28 (57%) Frame = +1 Query: 268 YEGGYYHGKIIFPREFPFKPPSIYMITP 351 YEG + + FP +P+ PP+I +P Sbjct: 73 YEGLKFKISMSFPANYPYSPPTIIFTSP 100 >SPBP35G2.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 1060 Score = 27.5 bits (58), Expect = 1.3 Identities = 15/45 (33%), Positives = 22/45 (48%) Frame = -2 Query: 404 MKVCNTEAQSCVCLELPFGVIM*IEGGLNGNSLGKIIFP**YPPS 270 +K+ + C +L I+ + LN NSL KI+F Y PS Sbjct: 970 LKIISISQGDTACGDLVVDAILDTQNDLNPNSLEKILFEQTYGPS 1014 >SPCC736.11 |ago1|csp9|argonaute|Schizosaccharomyces pombe|chr 3|||Manual Length = 834 Score = 25.8 bits (54), Expect = 4.0 Identities = 15/69 (21%), Positives = 36/69 (52%) Frame = +2 Query: 53 FAIKHFTRRTVNTQKLTIHVNMAKTKVTGATSRLKQDYLRLKNDPVPYVTAEPVPSNILE 232 ++I+ F+ ++ + +N + K++ A L+ +RL+ +P + + +E Sbjct: 261 YSIEGFSSKSASDSFFVRRLNGEEQKISVAEYFLENHNVRLQYPNLPCILVKNGAMLPIE 320 Query: 233 WHYVVKGRR 259 + +VVKG+R Sbjct: 321 FCFVVKGQR 329 >SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual Length = 151 Score = 25.4 bits (53), Expect = 5.3 Identities = 11/33 (33%), Positives = 18/33 (54%) Frame = +3 Query: 369 NTRLCLSITDFHPDTWNPAWSISTILTGLLSFM 467 N LCL I + W+P + ++ ILT + S + Sbjct: 84 NGELCLDILQ---NRWSPTYDVAAILTSIQSLL 113 Score = 24.6 bits (51), Expect = 9.2 Identities = 8/29 (27%), Positives = 17/29 (58%) Frame = +1 Query: 262 SPYEGGYYHGKIIFPREFPFKPPSIYMIT 348 +P+E G + + F ++P KPP + ++ Sbjct: 46 TPFEDGTFKLVLSFDEQYPNKPPLVKFVS 74 >SPAC140.01 |sdh2||succinate dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 252 Score = 25.4 bits (53), Expect = 5.3 Identities = 10/23 (43%), Positives = 15/23 (65%) Frame = +2 Query: 413 LEPCLVYFYDPYRSVEFYVGEDS 481 L P L YFY Y+S+E ++ D+ Sbjct: 120 LVPDLTYFYKQYKSIEPWLQNDN 142 >SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 419 Score = 25.4 bits (53), Expect = 5.3 Identities = 12/43 (27%), Positives = 22/43 (51%) Frame = +2 Query: 173 LKNDPVPYVTAEPVPSNILEWHYVVKGRRKVPTKGDIIMERLF 301 + +P ++ E VPS IL ++ + ++P K I+E F Sbjct: 66 IAENPYQSLSLESVPSKILFGSFLFYVKDRIPEKAGYIIEEQF 108 >SPCC645.03c |isa1||iron-sulfur protein Isa1|Schizosaccharomyces pombe|chr 3|||Manual Length = 190 Score = 25.4 bits (53), Expect = 5.3 Identities = 12/20 (60%), Positives = 13/20 (65%) Frame = -1 Query: 372 CLS*TAIRCYHVNRRWFERK 313 CLS A+RC VNR F RK Sbjct: 4 CLSKPALRCRSVNRLQFLRK 23 >SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces pombe|chr 1|||Manual Length = 897 Score = 25.0 bits (52), Expect = 7.0 Identities = 9/19 (47%), Positives = 13/19 (68%) Frame = +1 Query: 307 REFPFKPPSIYMITPNGSS 363 + FPF+PP+ TP+ SS Sbjct: 99 KNFPFRPPTRLTSTPSNSS 117 >SPBC27B12.11c |||transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 738 Score = 24.6 bits (51), Expect = 9.2 Identities = 9/21 (42%), Positives = 16/21 (76%), Gaps = 1/21 (4%) Frame = +3 Query: 294 DYFPKGISVQTTFDLH-DNTE 353 +Y+P+ +S+Q + D+H NTE Sbjct: 106 EYYPRSVSMQQSQDVHFQNTE 126 >SPBC336.01 |fbh1|fdh1, fdh|DNA helicase I|Schizosaccharomyces pombe|chr 2|||Manual Length = 878 Score = 24.6 bits (51), Expect = 9.2 Identities = 11/27 (40%), Positives = 15/27 (55%) Frame = -3 Query: 286 DNIPLRRDFSPPFNDVMPFQYVGRHRF 206 DN+ L DF P F D + F +G R+ Sbjct: 727 DNVQLGNDFRPKF-DSVSFSRIGSSRY 752 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,363,603 Number of Sequences: 5004 Number of extensions: 50640 Number of successful extensions: 153 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 140 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 153 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 216376042 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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