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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= prgv0826
         (528 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc...    76   4e-15
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch...    40   3e-04
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac...    37   0.002
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha...    37   0.002
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch...    36   0.003
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac...    35   0.007
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac...    35   0.007
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo...    32   0.061
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch...    31   0.080
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch...    30   0.19 
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2...    28   0.99 
SPBP35G2.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr...    27   1.3  
SPCC736.11 |ago1|csp9|argonaute|Schizosaccharomyces pombe|chr 3|...    26   4.0  
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz...    25   5.3  
SPAC140.01 |sdh2||succinate dehydrogenase |Schizosaccharomyces p...    25   5.3  
SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|...    25   5.3  
SPCC645.03c |isa1||iron-sulfur protein Isa1|Schizosaccharomyces ...    25   5.3  
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po...    25   7.0  
SPBC27B12.11c |||transcription factor |Schizosaccharomyces pombe...    25   9.2  
SPBC336.01 |fbh1|fdh1, fdh|DNA helicase I|Schizosaccharomyces po...    25   9.2  

>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
           Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 227

 Score = 75.8 bits (178), Expect = 4e-15
 Identities = 32/55 (58%), Positives = 40/55 (72%)
 Frame = +3

Query: 357 QFKTNTRLCLSITDFHPDTWNPAWSISTILTGLLSFMLEKTPTLGSIETSRYQKR 521
           +F+TNTRLCLS +DFHP +WNP+W +STIL GL+SFM     T G I TS   +R
Sbjct: 79  RFQTNTRLCLSFSDFHPKSWNPSWMVSTILVGLVSFMTSDEITTGGIVTSESTRR 133



 Score = 58.8 bits (136), Expect = 5e-10
 Identities = 22/32 (68%), Positives = 28/32 (87%)
 Frame = +1

Query: 262 SPYEGGYYHGKIIFPREFPFKPPSIYMITPNG 357
           +PYEGG YHG +IFP ++PFKPP+I MITP+G
Sbjct: 47  TPYEGGQYHGTLIFPPDYPFKPPAIRMITPSG 78



 Score = 52.8 bits (121), Expect = 3e-08
 Identities = 22/47 (46%), Positives = 30/47 (63%)
 Frame = +2

Query: 137 GATSRLKQDYLRLKNDPVPYVTAEPVPSNILEWHYVVKGRRKVPTKG 277
           GA  RL ++YL L+ +PV  V A+P   NILEWHY++ G    P +G
Sbjct: 5   GAYKRLMKEYLALQKNPVELVDAKPATENILEWHYIITGPPDTPYEG 51


>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
           Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 217

 Score = 39.5 bits (88), Expect = 3e-04
 Identities = 20/51 (39%), Positives = 29/51 (56%), Gaps = 4/51 (7%)
 Frame = +1

Query: 256 EKSPYEGGYYHGKIIFPREFPFKPPSIYMIT----PNGSSRQTHDCASVLQ 396
           E +PYEGGY+   I  P ++PF+PP +   T    PN SS+    C  +L+
Sbjct: 44  EGTPYEGGYFVVDIEIPIDYPFRPPKMNFDTKIYHPNVSSQTGAICLDILK 94


>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
           Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 167

 Score = 36.7 bits (81), Expect = 0.002
 Identities = 12/31 (38%), Positives = 20/31 (64%)
 Frame = +1

Query: 256 EKSPYEGGYYHGKIIFPREFPFKPPSIYMIT 348
           E + YEGG++H  + FP+++P  PP +   T
Sbjct: 46  EDTLYEGGFFHATLSFPQDYPLMPPKMKFTT 76


>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
           Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 147

 Score = 36.7 bits (81), Expect = 0.002
 Identities = 14/29 (48%), Positives = 18/29 (62%)
 Frame = +1

Query: 262 SPYEGGYYHGKIIFPREFPFKPPSIYMIT 348
           SPY GG +   I FP ++PFKPP +   T
Sbjct: 43  SPYAGGVFFLSIHFPTDYPFKPPKVNFTT 71



 Score = 27.9 bits (59), Expect = 0.99
 Identities = 13/39 (33%), Positives = 21/39 (53%)
 Frame = +3

Query: 366 TNTRLCLSITDFHPDTWNPAWSISTILTGLLSFMLEKTP 482
           +N  +CL I     D W+PA +IS +L  + S + +  P
Sbjct: 80  SNGSICLDILR---DQWSPALTISKVLLSICSLLTDPNP 115


>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
           Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 148

 Score = 36.3 bits (80), Expect = 0.003
 Identities = 12/31 (38%), Positives = 21/31 (67%)
 Frame = +1

Query: 256 EKSPYEGGYYHGKIIFPREFPFKPPSIYMIT 348
           ++S YEGG +H ++  P E+P  PP++  +T
Sbjct: 42  QQSAYEGGKFHLELFLPDEYPMMPPNVRFLT 72


>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
           Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 160

 Score = 35.1 bits (77), Expect = 0.007
 Identities = 21/76 (27%), Positives = 34/76 (44%)
 Frame = +3

Query: 240 TSLKGGEKSLRRGILSWKDYFPKGISVQTTFDLHDNTEWQFKTNTRLCLSITDFHPDTWN 419
           T  +GG+  L   +       P  +  QT   +H N  W   TN  +C+ I   H   W+
Sbjct: 47  TPYEGGQWVLDIHVHEGYPISPPSVYFQTKI-VHPNISW---TNGEVCMDILKTH---WS 99

Query: 420 PAWSISTILTGLLSFM 467
           PAWS+ +    ++S +
Sbjct: 100 PAWSLQSACLAIISLL 115



 Score = 32.3 bits (70), Expect = 0.046
 Identities = 17/51 (33%), Positives = 26/51 (50%), Gaps = 4/51 (7%)
 Frame = +1

Query: 259 KSPYEGGYYHGKIIFPREFPFKPPSIY----MITPNGSSRQTHDCASVLQT 399
           ++PYEGG +   I     +P  PPS+Y    ++ PN S      C  +L+T
Sbjct: 46  ETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPNISWTNGEVCMDILKT 96


>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
           Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 155

 Score = 35.1 bits (77), Expect = 0.007
 Identities = 18/49 (36%), Positives = 29/49 (59%), Gaps = 4/49 (8%)
 Frame = +1

Query: 262 SPYEGGYYHGKIIFPREFPFKPPSIYMIT----PNGSSRQTHDCASVLQ 396
           S Y GG +H  + FP ++PF+PP+I   T    PN  S + + C ++L+
Sbjct: 49  SVYAGGKFHFSLKFPLDYPFQPPTIEFTTRIYHPNFDS-EGNVCLAILK 96


>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
           Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 166

 Score = 31.9 bits (69), Expect = 0.061
 Identities = 10/25 (40%), Positives = 17/25 (68%)
 Frame = +1

Query: 262 SPYEGGYYHGKIIFPREFPFKPPSI 336
           +P+EGG Y   + FP ++P  PP++
Sbjct: 48  TPFEGGLYPATLKFPSDYPLGPPTL 72


>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
           Hus5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 157

 Score = 31.5 bits (68), Expect = 0.080
 Identities = 12/31 (38%), Positives = 17/31 (54%)
 Frame = +1

Query: 259 KSPYEGGYYHGKIIFPREFPFKPPSIYMITP 351
           K+ +EGG Y   + FP E+P +PP      P
Sbjct: 50  KTSWEGGLYKLTMAFPEEYPTRPPKCRFTPP 80


>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
           Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 184

 Score = 30.3 bits (65), Expect = 0.19
 Identities = 11/26 (42%), Positives = 17/26 (65%)
 Frame = +1

Query: 259 KSPYEGGYYHGKIIFPREFPFKPPSI 336
           ++PY GG +   +  P E+P+K PSI
Sbjct: 41  ETPYSGGIWKVHVELPSEYPWKSPSI 66


>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 176

 Score = 27.9 bits (59), Expect = 0.99
 Identities = 10/28 (35%), Positives = 16/28 (57%)
 Frame = +1

Query: 268 YEGGYYHGKIIFPREFPFKPPSIYMITP 351
           YEG  +   + FP  +P+ PP+I   +P
Sbjct: 73  YEGLKFKISMSFPANYPYSPPTIIFTSP 100


>SPBP35G2.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 1060

 Score = 27.5 bits (58), Expect = 1.3
 Identities = 15/45 (33%), Positives = 22/45 (48%)
 Frame = -2

Query: 404  MKVCNTEAQSCVCLELPFGVIM*IEGGLNGNSLGKIIFP**YPPS 270
            +K+ +       C +L    I+  +  LN NSL KI+F   Y PS
Sbjct: 970  LKIISISQGDTACGDLVVDAILDTQNDLNPNSLEKILFEQTYGPS 1014


>SPCC736.11 |ago1|csp9|argonaute|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 834

 Score = 25.8 bits (54), Expect = 4.0
 Identities = 15/69 (21%), Positives = 36/69 (52%)
 Frame = +2

Query: 53  FAIKHFTRRTVNTQKLTIHVNMAKTKVTGATSRLKQDYLRLKNDPVPYVTAEPVPSNILE 232
           ++I+ F+ ++ +       +N  + K++ A   L+   +RL+   +P +  +      +E
Sbjct: 261 YSIEGFSSKSASDSFFVRRLNGEEQKISVAEYFLENHNVRLQYPNLPCILVKNGAMLPIE 320

Query: 233 WHYVVKGRR 259
           + +VVKG+R
Sbjct: 321 FCFVVKGQR 329


>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
           E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 151

 Score = 25.4 bits (53), Expect = 5.3
 Identities = 11/33 (33%), Positives = 18/33 (54%)
 Frame = +3

Query: 369 NTRLCLSITDFHPDTWNPAWSISTILTGLLSFM 467
           N  LCL I     + W+P + ++ ILT + S +
Sbjct: 84  NGELCLDILQ---NRWSPTYDVAAILTSIQSLL 113



 Score = 24.6 bits (51), Expect = 9.2
 Identities = 8/29 (27%), Positives = 17/29 (58%)
 Frame = +1

Query: 262 SPYEGGYYHGKIIFPREFPFKPPSIYMIT 348
           +P+E G +   + F  ++P KPP +  ++
Sbjct: 46  TPFEDGTFKLVLSFDEQYPNKPPLVKFVS 74


>SPAC140.01 |sdh2||succinate dehydrogenase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 252

 Score = 25.4 bits (53), Expect = 5.3
 Identities = 10/23 (43%), Positives = 15/23 (65%)
 Frame = +2

Query: 413 LEPCLVYFYDPYRSVEFYVGEDS 481
           L P L YFY  Y+S+E ++  D+
Sbjct: 120 LVPDLTYFYKQYKSIEPWLQNDN 142


>SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 419

 Score = 25.4 bits (53), Expect = 5.3
 Identities = 12/43 (27%), Positives = 22/43 (51%)
 Frame = +2

Query: 173 LKNDPVPYVTAEPVPSNILEWHYVVKGRRKVPTKGDIIMERLF 301
           +  +P   ++ E VPS IL   ++   + ++P K   I+E  F
Sbjct: 66  IAENPYQSLSLESVPSKILFGSFLFYVKDRIPEKAGYIIEEQF 108


>SPCC645.03c |isa1||iron-sulfur protein Isa1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 190

 Score = 25.4 bits (53), Expect = 5.3
 Identities = 12/20 (60%), Positives = 13/20 (65%)
 Frame = -1

Query: 372 CLS*TAIRCYHVNRRWFERK 313
           CLS  A+RC  VNR  F RK
Sbjct: 4   CLSKPALRCRSVNRLQFLRK 23


>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 897

 Score = 25.0 bits (52), Expect = 7.0
 Identities = 9/19 (47%), Positives = 13/19 (68%)
 Frame = +1

Query: 307 REFPFKPPSIYMITPNGSS 363
           + FPF+PP+    TP+ SS
Sbjct: 99  KNFPFRPPTRLTSTPSNSS 117


>SPBC27B12.11c |||transcription factor |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 738

 Score = 24.6 bits (51), Expect = 9.2
 Identities = 9/21 (42%), Positives = 16/21 (76%), Gaps = 1/21 (4%)
 Frame = +3

Query: 294 DYFPKGISVQTTFDLH-DNTE 353
           +Y+P+ +S+Q + D+H  NTE
Sbjct: 106 EYYPRSVSMQQSQDVHFQNTE 126


>SPBC336.01 |fbh1|fdh1, fdh|DNA helicase I|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 878

 Score = 24.6 bits (51), Expect = 9.2
 Identities = 11/27 (40%), Positives = 15/27 (55%)
 Frame = -3

Query: 286 DNIPLRRDFSPPFNDVMPFQYVGRHRF 206
           DN+ L  DF P F D + F  +G  R+
Sbjct: 727 DNVQLGNDFRPKF-DSVSFSRIGSSRY 752


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,363,603
Number of Sequences: 5004
Number of extensions: 50640
Number of successful extensions: 153
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 140
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 153
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 216376042
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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