BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0805 (600 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 118 3e-27 At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 93 1e-19 At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 93 1e-19 At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 91 5e-19 At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 71 4e-13 At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 33 0.11 At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 33 0.11 At2g40070.1 68415.m04923 expressed protein 31 0.58 At5g38660.1 68418.m04675 expressed protein similar to unknown pr... 31 0.77 At1g34360.1 68414.m04266 translation initiation factor 3 (IF-3) ... 29 2.4 At2g35490.1 68415.m04347 plastid-lipid associated protein PAP, p... 28 5.4 At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /... 27 7.2 At3g63180.1 68416.m07097 expressed protein 27 7.2 At3g50840.1 68416.m05567 phototropic-responsive NPH3 family prot... 27 9.5 At1g65910.1 68414.m07479 no apical meristem (NAM) family protein... 27 9.5 At1g09140.1 68414.m01018 SF2/ASF-like splicing modulator (SRP30)... 27 9.5 At1g03930.1 68414.m00378 protein kinase (ADK1) identical to dual... 27 9.5 >At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, putative similar to ELONGATION FACTOR 2 GB:O14460 from [Schizosaccharomyces pombe] Length = 843 Score = 118 bits (284), Expect = 3e-27 Identities = 50/75 (66%), Positives = 62/75 (82%) Frame = +2 Query: 11 EVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQC 190 E A+GGIY VLN++RGHVFEE Q GTP++ +KAYLPV ESFGF++ LR+ T GQAFPQC Sbjct: 738 EGALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVVESFGFSSQLRAATSGQAFPQC 797 Query: 191 VFDHWQVLPGDPCEP 235 VFDHW+++ DP EP Sbjct: 798 VFDHWEMMSSDPLEP 812 >At1g06220.2 68414.m00656 elongation factor Tu family protein similar to Cryptosporidium parvum elongation factor-2 GB:U21667 GI:706974 from [Cryptosporidium parvum] Length = 987 Score = 93.5 bits (222), Expect = 1e-19 Identities = 43/71 (60%), Positives = 51/71 (71%) Frame = +2 Query: 20 VGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFD 199 V IY VL+RRRGHV + GTP +IVKA+LPV ESFGF DLR +T GQAF VFD Sbjct: 857 VTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFD 916 Query: 200 HWQVLPGDPCE 232 HW ++PGDP + Sbjct: 917 HWAIVPGDPLD 927 >At1g06220.1 68414.m00655 elongation factor Tu family protein similar to Cryptosporidium parvum elongation factor-2 GB:U21667 GI:706974 from [Cryptosporidium parvum] Length = 987 Score = 93.5 bits (222), Expect = 1e-19 Identities = 43/71 (60%), Positives = 51/71 (71%) Frame = +2 Query: 20 VGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFD 199 V IY VL+RRRGHV + GTP +IVKA+LPV ESFGF DLR +T GQAF VFD Sbjct: 857 VTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFD 916 Query: 200 HWQVLPGDPCE 232 HW ++PGDP + Sbjct: 917 HWAIVPGDPLD 927 >At5g25230.1 68418.m02991 elongation factor Tu family protein translation Elongation Factor 2, Schizosaccharomyces pombe, PIR:T39902 Length = 973 Score = 91.1 bits (216), Expect = 5e-19 Identities = 42/71 (59%), Positives = 51/71 (71%) Frame = +2 Query: 20 VGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFD 199 V IY VL+RRRG+V + GTP +IVKA+LPV ESFGF DLR +T GQAF VFD Sbjct: 843 VTAIYTVLSRRRGYVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFD 902 Query: 200 HWQVLPGDPCE 232 HW ++PGDP + Sbjct: 903 HWAIVPGDPLD 913 >At3g22980.1 68416.m02898 elongation factor Tu family protein similar to eukaryotic translation elongation factor 2 GB:NP_001952 [Homo sapiens] Length = 1015 Score = 71.3 bits (167), Expect = 4e-13 Identities = 32/74 (43%), Positives = 45/74 (60%) Frame = +2 Query: 5 TSEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFP 184 T+ +G +Y VL+RRR + +E G+ +F V AY+PV+ESFGF +LR T G A Sbjct: 885 TAPEYLGPMYAVLSRRRARILKEEMQEGSSLFTVHAYVPVSESFGFADELRKGTSGGASA 944 Query: 185 QCVFDHWQVLPGDP 226 V HW++L DP Sbjct: 945 LMVLSHWEMLEEDP 958 >At2g45030.1 68415.m05606 mitochondrial elongation factor, putative similar to SP|P25039 Elongation factor G 1, mitochondrial precursor (mEF-G-1) {Saccharomyces cerevisiae}; contains Pfam profiles PF00009: Elongation factor Tu GTP binding domain, PF03764: Elongation factor G domain IV, PF00679: Elongation factor G C-terminus Length = 754 Score = 33.5 bits (73), Expect = 0.11 Identities = 16/51 (31%), Positives = 28/51 (54%) Frame = +2 Query: 23 GGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQ 175 G + G +N+R+G + Q ++ A +P+N FG++ LRS T G+ Sbjct: 674 GTVAGDINKRKGIIVGNDQEGDDS--VITANVPLNNMFGYSTSLRSMTQGK 722 >At1g45332.1 68414.m05195 mitochondrial elongation factor, putative similar to mitochondrial elongation factor GI:3917 from [Saccharomyces cerevisiae] Length = 754 Score = 33.5 bits (73), Expect = 0.11 Identities = 16/51 (31%), Positives = 28/51 (54%) Frame = +2 Query: 23 GGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQ 175 G + G +N+R+G + Q ++ A +P+N FG++ LRS T G+ Sbjct: 674 GTVAGDINKRKGIIVGNDQEGDDS--VITANVPLNNMFGYSTSLRSMTQGK 722 >At2g40070.1 68415.m04923 expressed protein Length = 607 Score = 31.1 bits (67), Expect = 0.58 Identities = 19/47 (40%), Positives = 23/47 (48%), Gaps = 1/47 (2%) Frame = +3 Query: 162 TPADRPSRSAYSTIGRSSLETRANL-RASPTTCTGNEKEERIEGRSP 299 TP RP+ TI RSS TR + AS T T N +I+ SP Sbjct: 297 TPTSRPTLPPSKTISRSSTPTRRPIASASAATTTANPTISQIKPSSP 343 >At5g38660.1 68418.m04675 expressed protein similar to unknown protein (pir||S75762) Length = 286 Score = 30.7 bits (66), Expect = 0.77 Identities = 15/56 (26%), Positives = 34/56 (60%), Gaps = 4/56 (7%) Frame = -3 Query: 286 SILSSFSFPVHVVGLALRFARVSREDLPMVEYA----LREGLSAGVGTQIGSKTER 131 +I+ ++ FP+ ++G+AL++A + + +P + Y+ LRE + + TQ+ + R Sbjct: 126 AIMLTYGFPLSIIGMALKYAEL--KPVPCLSYSDAVKLRESCATPILTQVRNDVTR 179 >At1g34360.1 68414.m04266 translation initiation factor 3 (IF-3) family protein low similarity to Translation initiation factor IF-3 from [subsp. Schizaphis graminum] {Buchnera aphidicola} SP|P46243, {Salmonella typhimurium} SP|P33321; contains Pfam profiles PF05198: Translation initiation factor IF-3 N-terminal domain, PF00707: Translation initiation factor IF-3 C-terminal domain Length = 520 Score = 29.1 bits (62), Expect = 2.4 Identities = 18/51 (35%), Positives = 26/51 (50%), Gaps = 1/51 (1%) Frame = +3 Query: 231 NLRASPTTCTGNEKEERIEGRSPRLN-SIFGQIVNYIVKAN*KNVSIRKAL 380 NLR T+ +KE ++ PRLN I G V + + VS+R+AL Sbjct: 62 NLRFLATSAQTRKKEAEVDSDGPRLNEKITGDYVRLVSEEGHCVVSLREAL 112 >At2g35490.1 68415.m04347 plastid-lipid associated protein PAP, putative similar to plastid-lipid associated protein PAP3 [Brassica rapa] GI:14248552; contains Pfam profile PF04755: PAP_fibrillin Length = 376 Score = 27.9 bits (59), Expect = 5.4 Identities = 12/22 (54%), Positives = 13/22 (59%) Frame = +2 Query: 191 VFDHWQVLPGDPCEPQSKPYNV 256 V D W PGD EP S+P NV Sbjct: 71 VTDEWGEKPGDANEPDSQPDNV 92 >At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein weak similarity to polygalacturonase PG1 [Glycine max] GI:5669846; contains PF00295: Glycosyl hydrolases family 28 Length = 444 Score = 27.5 bits (58), Expect = 7.2 Identities = 11/22 (50%), Positives = 17/22 (77%), Gaps = 1/22 (4%) Frame = +1 Query: 52 SWSRFRRVPGGRY-TYVHCEGL 114 S+ R R +PGGRY +++H +GL Sbjct: 123 SYGRGRELPGGRYMSFIHGDGL 144 >At3g63180.1 68416.m07097 expressed protein Length = 978 Score = 27.5 bits (58), Expect = 7.2 Identities = 10/35 (28%), Positives = 21/35 (60%) Frame = +3 Query: 111 PTYLSMSRSVLLPICVPTPADRPSRSAYSTIGRSS 215 P ++ + L P+ VP+ + RP++S + +G +S Sbjct: 539 PAFIYPANHHLQPVMVPSKSSRPTKSPHLAVGLAS 573 >At3g50840.1 68416.m05567 phototropic-responsive NPH3 family protein contains NPH3 family domain, Pfam:PF03000 Length = 569 Score = 27.1 bits (57), Expect = 9.5 Identities = 23/78 (29%), Positives = 35/78 (44%), Gaps = 4/78 (5%) Frame = +3 Query: 66 SKSPRWQVHLCSL*RPTY----LSMSRSVLLPICVPTPADRPSRSAYSTIGRSSLETRAN 233 SK+ W L L P + LSM SVL P V ++ I RSS + ++ Sbjct: 191 SKTELWFEDLTELSFPIFRRVILSMKSSVLSPEIVERSLLTYAKKHIPGISRSSSASSSS 250 Query: 234 LRASPTTCTGNEKEERIE 287 +S T + N++ E +E Sbjct: 251 SSSSTTIASENQQRELLE 268 >At1g65910.1 68414.m07479 no apical meristem (NAM) family protein similar to jasmonic acid 2 GI:6175246 from [Lycopersicon esculentum]; similar to NAC2 (GI:6456751) {Arabidopsis thaliana} Length = 631 Score = 27.1 bits (57), Expect = 9.5 Identities = 16/58 (27%), Positives = 28/58 (48%), Gaps = 2/58 (3%) Frame = -3 Query: 274 SFSFPVHVVGLALRFARVSREDLPMVEYALREGLSAG--VGTQIGSKTERLIDR*VGL 107 + S+P V +AL AR+ LP V EGL+ G + + T+ ++ + + L Sbjct: 249 TISYPPSKVDIALECARLQNRMLPPVPPLYVEGLTHNEYFGNNVANDTDEMLSKIIAL 306 >At1g09140.1 68414.m01018 SF2/ASF-like splicing modulator (SRP30) nearly identical to SF2/ASF-like splicing modulator Srp30 [Arabidopsis thaliana] GI:4775270 Length = 268 Score = 27.1 bits (57), Expect = 9.5 Identities = 18/46 (39%), Positives = 26/46 (56%), Gaps = 3/46 (6%) Frame = +3 Query: 114 TYLSMSRSVLLPICVPTPADRP---SRSAYSTIGRSSLETRANLRA 242 +Y S SRSV P +P RP SRS YS++ RS +++ R+ Sbjct: 212 SYSSKSRSVS-PARSISPRSRPLSRSRSLYSSVSRSQSRSKSRSRS 256 >At1g03930.1 68414.m00378 protein kinase (ADK1) identical to dual specificity kinase 1 (ADK1) [Arabidopsis thaliana] gi|1216484|gb|AAB47968; supported by cDNA gi:18700076 and gi:1216483. Note: differences between cDNAs in the 11th exon, possibly due to errors or alternative splicing. Length = 471 Score = 27.1 bits (57), Expect = 9.5 Identities = 16/41 (39%), Positives = 21/41 (51%), Gaps = 2/41 (4%) Frame = +3 Query: 210 SSLETRANLRASPTTCTGN--EKEERIEGRSPRLNSIFGQI 326 SS TR + A+P G EK+ERI G+ R N G + Sbjct: 304 SSSRTRNHTTANPGLTAGASLEKQERIAGKETRENRFSGAV 344 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,685,441 Number of Sequences: 28952 Number of extensions: 267685 Number of successful extensions: 685 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 674 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 685 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1187288784 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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