BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0693 (648 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 142 3e-35 SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 142 3e-35 SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 142 3e-35 SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 48 2e-06 SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 29 0.58 SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2... 26 4.1 SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr 1|... 26 5.4 SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 7.1 >SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual Length = 460 Score = 142 bits (345), Expect = 3e-35 Identities = 62/90 (68%), Positives = 74/90 (82%) Frame = +2 Query: 2 NNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVN 181 N+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E + Sbjct: 329 NDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEES 388 Query: 182 PKSIKSGDAAIVNLVPSKPLCVESSRNSHP 271 PK +KSGDA I +VPSKP+CVE+ + P Sbjct: 389 PKFVKSGDACIAKMVPSKPMCVEAFTDYAP 418 Score = 49.2 bits (112), Expect = 5e-07 Identities = 24/32 (75%), Positives = 26/32 (81%), Gaps = 1/32 (3%) Frame = +1 Query: 241 MC-RVLQEFPPLGRFAVRDMRQTVAVGVIKAV 333 MC ++ PLGRFAVRDMRQTVAVGVIKAV Sbjct: 408 MCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439 >SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 142 bits (345), Expect = 3e-35 Identities = 62/90 (68%), Positives = 74/90 (82%) Frame = +2 Query: 2 NNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVN 181 N+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E + Sbjct: 329 NDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEES 388 Query: 182 PKSIKSGDAAIVNLVPSKPLCVESSRNSHP 271 PK +KSGDA I +VPSKP+CVE+ + P Sbjct: 389 PKFVKSGDACIAKMVPSKPMCVEAFTDYAP 418 Score = 49.2 bits (112), Expect = 5e-07 Identities = 24/32 (75%), Positives = 26/32 (81%), Gaps = 1/32 (3%) Frame = +1 Query: 241 MC-RVLQEFPPLGRFAVRDMRQTVAVGVIKAV 333 MC ++ PLGRFAVRDMRQTVAVGVIKAV Sbjct: 408 MCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439 >SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual Length = 460 Score = 142 bits (345), Expect = 3e-35 Identities = 62/90 (68%), Positives = 74/90 (82%) Frame = +2 Query: 2 NNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVN 181 N+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E + Sbjct: 329 NDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEES 388 Query: 182 PKSIKSGDAAIVNLVPSKPLCVESSRNSHP 271 PK +KSGDA I +VPSKP+CVE+ + P Sbjct: 389 PKFVKSGDACIAKMVPSKPMCVEAFTDYAP 418 Score = 49.2 bits (112), Expect = 5e-07 Identities = 24/32 (75%), Positives = 26/32 (81%), Gaps = 1/32 (3%) Frame = +1 Query: 241 MC-RVLQEFPPLGRFAVRDMRQTVAVGVIKAV 333 MC ++ PLGRFAVRDMRQTVAVGVIKAV Sbjct: 408 MCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 47.6 bits (108), Expect = 2e-06 Identities = 25/82 (30%), Positives = 40/82 (48%) Frame = +2 Query: 5 NPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNP 184 NP F AQ+ +L P ++ GY+ V+ HTA FA++ K+D +T + ++ P Sbjct: 552 NPVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHKLD-KTNRKSKKPP 610 Query: 185 KSIKSGDAAIVNLVPSKPLCVE 250 G I L P+C+E Sbjct: 611 MFATKGMKIIAELETQTPVCME 632 Score = 26.6 bits (56), Expect = 3.1 Identities = 12/28 (42%), Positives = 20/28 (71%), Gaps = 1/28 (3%) Frame = +1 Query: 256 QEFPPLGRFAVRDMRQTVAVG-VIKAVN 336 +++ +GRF +RD TVAVG V+K ++ Sbjct: 635 EDYQYMGRFTLRDQGTTVAVGKVVKILD 662 >SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 592 Score = 29.1 bits (62), Expect = 0.58 Identities = 12/24 (50%), Positives = 16/24 (66%) Frame = +1 Query: 256 QEFPPLGRFAVRDMRQTVAVGVIK 327 +E P LGRF +R TVA G++K Sbjct: 566 EECPALGRFILRRSGDTVAAGIVK 589 >SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 26.2 bits (55), Expect = 4.1 Identities = 13/49 (26%), Positives = 23/49 (46%) Frame = -2 Query: 392 PLVAFSAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWRTL 246 P + ++ T+PP S+ T + PT ++ + TA NS T+ Sbjct: 73 PSTSHNSTTTTVPPTTSMNTTTTVPPTTSLNTTTTTAPPTTHVNSTTTV 121 >SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1372 Score = 25.8 bits (54), Expect = 5.4 Identities = 12/46 (26%), Positives = 24/46 (52%) Frame = +2 Query: 140 EKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESSRNSHPSV 277 EKV+ + + + +S+K D I N S + +E+ +N+ P + Sbjct: 36 EKVEAKALSRSRLKNQSVKKTDLRITNDYSSLFVSIENKKNTIPDI 81 >SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 268 Score = 25.4 bits (53), Expect = 7.1 Identities = 11/25 (44%), Positives = 18/25 (72%) Frame = -1 Query: 213 MAASPDLMDFGLTSVDLPVRRSTFS 139 ++ +PDL D L+SVD P++ +T S Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,507,389 Number of Sequences: 5004 Number of extensions: 48983 Number of successful extensions: 144 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 138 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 144 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 291768710 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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