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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= prgv0684
         (689 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g17820.1 68416.m02272 glutamine synthetase (GS1) identical to...    66   2e-11
At5g35630.1 68418.m04253 glutamine synthetase (GS2) identical to...    66   3e-11
At5g37600.1 68418.m04529 glutamine synthetase, putative similar ...    64   8e-11
At1g66200.1 68414.m07514 glutamine synthetase, putative similar ...    64   8e-11
At5g16570.1 68418.m01939 glutamine synthetase, putative similar ...    63   1e-10
At1g48470.1 68414.m05418 glutamine synthetase, putative similar ...    63   1e-10
At3g18630.1 68416.m02367 uracil DNA glycosylase family protein c...    33   0.24 
At1g49610.1 68414.m05562 F-box family protein contains F-box dom...    31   0.95 
At4g26580.1 68417.m03832 zinc finger (C3HC4-type RING finger) fa...    27   8.9  

>At3g17820.1 68416.m02272 glutamine synthetase (GS1) identical to
           glutamine synthetase, cytosolic isozyme (glutamate--
           ammonia ligase, GS1) [Arabidopsis thaliana]
           SWISS-PROT:Q9LVI8
          Length = 354

 Score = 66.5 bits (155), Expect = 2e-11
 Identities = 30/49 (61%), Positives = 36/49 (73%)
 Frame = +1

Query: 1   RHETGLHETASINDFSAGVANRGSSIRIPRSVAEDKKGYLEDRRPASKL 147
           R  TG HETA IN FS GVANRG+S+R+ R   ++ KGY EDRRPAS +
Sbjct: 290 RRLTGKHETADINTFSWGVANRGASVRVGRDTEKEGKGYFEDRRPASNM 338


>At5g35630.1 68418.m04253 glutamine synthetase (GS2) identical to
           glutamine synthetase, chloroplast precursor (glutamate--
           ammonia ligase, GS2) [Arabidopsis thaliana]
           SWISS-PROT:Q43127
          Length = 430

 Score = 65.7 bits (153), Expect = 3e-11
 Identities = 32/49 (65%), Positives = 35/49 (71%)
 Frame = +1

Query: 1   RHETGLHETASINDFSAGVANRGSSIRIPRSVAEDKKGYLEDRRPASKL 147
           R  TG HETASI+ FS GVANRG SIR+ R      KGYLEDRRPAS +
Sbjct: 348 RRLTGKHETASIDQFSWGVANRGCSIRVGRDTEAKGKGYLEDRRPASNM 396


>At5g37600.1 68418.m04529 glutamine synthetase, putative similar to
           glutamine synthetase, cytosolic isozyme (Glutamate--
           ammonia ligase, GS1) [Lotus japonicus] SWISS-PROT:Q42899
          Length = 356

 Score = 64.1 bits (149), Expect = 8e-11
 Identities = 30/49 (61%), Positives = 35/49 (71%)
 Frame = +1

Query: 1   RHETGLHETASINDFSAGVANRGSSIRIPRSVAEDKKGYLEDRRPASKL 147
           R  TG HETA IN F  GVANRG+SIR+ R   ++ KGY EDRRPAS +
Sbjct: 290 RRLTGHHETADINTFLWGVANRGASIRVGRDTEKEGKGYFEDRRPASNM 338


>At1g66200.1 68414.m07514 glutamine synthetase, putative similar to
           glutamine synthetase, cytosolic isozyme (Glutamate--
           ammonia ligase, GS1) [Lotus japonicus] SWISS-PROT:Q42899
          Length = 356

 Score = 64.1 bits (149), Expect = 8e-11
 Identities = 30/49 (61%), Positives = 35/49 (71%)
 Frame = +1

Query: 1   RHETGLHETASINDFSAGVANRGSSIRIPRSVAEDKKGYLEDRRPASKL 147
           R  TG HETA IN F  GVANRG+SIR+ R   ++ KGY EDRRPAS +
Sbjct: 290 RRLTGHHETADINTFLWGVANRGASIRVGRDTEKEGKGYFEDRRPASNM 338


>At5g16570.1 68418.m01939 glutamine synthetase, putative similar to
           glutamine synthetase, cytosolic isozyme (glutamate--
           ammonia ligase) [Alfalfa] SWISS-PROT:P04078
          Length = 356

 Score = 63.3 bits (147), Expect = 1e-10
 Identities = 30/49 (61%), Positives = 34/49 (69%)
 Frame = +1

Query: 1   RHETGLHETASINDFSAGVANRGSSIRIPRSVAEDKKGYLEDRRPASKL 147
           R  TG HETA IN F  GVANRG+SIR+ R   +  KGY EDRRPAS +
Sbjct: 290 RRLTGKHETADINTFLWGVANRGASIRVGRDTEQAGKGYFEDRRPASNM 338


>At1g48470.1 68414.m05418 glutamine synthetase, putative similar to
           glutamine synthetase, cytosolic isozyme (glutamate--
           ammonia ligase, GS1) [Arabidopsis thaliana]
           SWISS-PROT:Q9LVI8
          Length = 353

 Score = 63.3 bits (147), Expect = 1e-10
 Identities = 28/49 (57%), Positives = 36/49 (73%)
 Frame = +1

Query: 1   RHETGLHETASINDFSAGVANRGSSIRIPRSVAEDKKGYLEDRRPASKL 147
           R  TG HETA IN FS GVA+RG+S+R+ R   ++ KGY EDRRP+S +
Sbjct: 290 RRLTGKHETADINTFSWGVADRGASVRVGRDTEKEGKGYFEDRRPSSNM 338


>At3g18630.1 68416.m02367 uracil DNA glycosylase family protein
           contains Pfam profile: PF03167 uracil DNA glycosylase
           superfamily
          Length = 330

 Score = 32.7 bits (71), Expect = 0.24
 Identities = 12/24 (50%), Positives = 18/24 (75%)
 Frame = +2

Query: 380 YLDRHVIYNALNNGPFKDIKTIIV 451
           Y  +H+I+NALN  PF  +KT+I+
Sbjct: 147 YPPQHLIFNALNTTPFDRVKTVII 170


>At1g49610.1 68414.m05562 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 354

 Score = 30.7 bits (66), Expect = 0.95
 Identities = 25/87 (28%), Positives = 38/87 (43%)
 Frame = -3

Query: 300 GINLPYAYGELQFFRYTWAQQLSYTSFLFQCYSFKNYKYRMSAIDHQSTGPQF*SRSTIF 121
           GINLPY  G ++F      + L + SF F+ Y   +Y Y  S++   S   +  S   I 
Sbjct: 103 GINLPYIDGWIKFAMSRNVENL-FLSFDFRLYDVPDYLYINSSVKQLSIA-KILSGCPII 160

Query: 120 QITLLIFSDRARYSDTATTISHACTEV 40
           +   L F D+    D   + S    E+
Sbjct: 161 ESLTLHFCDQLMVLDLTKSPSLKILEI 187


>At4g26580.1 68417.m03832 zinc finger (C3HC4-type RING finger)
           family protein contains Pfam profile: PF00097 zinc
           finger, C3HC4 type (RING finger)
          Length = 464

 Score = 27.5 bits (58), Expect = 8.9
 Identities = 13/44 (29%), Positives = 24/44 (54%)
 Frame = -3

Query: 420 PLLSALYITCLSKYLLLCSAYEGFVYYIPFCTVPFIPLFRGINL 289
           P+L  L I+ L+ +  LC ++   ++ +  C VP +  F G N+
Sbjct: 336 PILHVLCISLLA-WNALCYSFPFLLFLLLCCVVPLVSSFLGYNM 378


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,295,580
Number of Sequences: 28952
Number of extensions: 220509
Number of successful extensions: 472
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 467
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 472
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1467502800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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