BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0676 (694 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC20G8.10c ||SPAC3A12.01c|beclin family protein|Schizosaccharo... 80 3e-16 SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase Abc... 28 1.5 SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces pombe... 27 2.6 SPAC20G8.01 |cdc17||ATP-dependent DNA ligase Cdc17|Schizosacchar... 26 5.9 SPAC959.02 |sec17||alpha SNAP |Schizosaccharomyces pombe|chr 1||... 26 5.9 SPAC1687.22c |puf3|SPAC222.02c|RNA-binding protein Puf3 |Schizos... 26 5.9 SPCC417.06c |ppk35|mug27|serine/threonine protein kinase Ppk35|S... 26 5.9 SPAC15A10.04c |zpr1||zinc finger protein Zpr1|Schizosaccharomyce... 25 7.9 SPAC1F7.02c |||ATP-dependent RNA helicase Has1 |Schizosaccharomy... 25 7.9 SPBC11C11.09c |rpl502|rpl5-2, rpl5b|60S ribosomal protein L5|Sch... 25 7.9 SPBC29A10.07 |||nucleoporin Pom152|Schizosaccharomyces pombe|chr... 25 7.9 SPBC1604.18c |||vacuolar sorting protein |Schizosaccharomyces po... 25 7.9 >SPAC20G8.10c ||SPAC3A12.01c|beclin family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 464 Score = 80.2 bits (189), Expect = 3e-16 Identities = 42/93 (45%), Positives = 58/93 (62%), Gaps = 8/93 (8%) Frame = +3 Query: 255 RLGRLPTAPVDWSEINAAWGQTVLLLSSLARKINFNFQRYKLVPYGNHSYIEVLE----D 422 RLGRLP+ V+W+EINAAWG TVLLL L K++F+ Y+L P+G+ S+I + Sbjct: 303 RLGRLPSQKVNWAEINAAWGMTVLLLDVLTEKLDFHSSSYQLKPFGSQSFIIRFDRDPNG 362 Query: 423 QKVLP----LYGSGGFRFLWDTKFDAAMVAFLD 509 +V P L+ SG + + +FD MVAFLD Sbjct: 363 NQVKPTKLDLFSSGELKIFMNRRFDQGMVAFLD 395 Score = 30.7 bits (66), Expect = 0.21 Identities = 14/53 (26%), Positives = 27/53 (50%) Frame = +1 Query: 64 EQDVYWREYTKYRKDLMTTEDQMKFYECQLAYTQSQLEKLKKTNVFKATFHIS 222 E+ ++ +++ ++ E Q + + +LEKL+K NVF F+IS Sbjct: 233 EEQEFYNNLNQFQIKKLSLERQYDCANLEFEHNSRKLEKLQKMNVFSDIFYIS 285 >SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase Abc2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1463 Score = 27.9 bits (59), Expect = 1.5 Identities = 15/38 (39%), Positives = 19/38 (50%), Gaps = 6/38 (15%) Frame = -2 Query: 531 CSLNCCRHLKMLPLPHRIWYP------RETGNHPNRIT 436 CSL C +P +R+WYP ETG P+R T Sbjct: 141 CSLVCLLAEIYVPPANRVWYPDDAAELEETGLRPSRFT 178 >SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces pombe|chr 2|||Manual Length = 230 Score = 27.1 bits (57), Expect = 2.6 Identities = 20/69 (28%), Positives = 39/69 (56%), Gaps = 2/69 (2%) Frame = -1 Query: 484 SNLVSQRNRKP-PEPYNGKTFWSSKTSMYE*FPYGTNL*RWKLKLIFLANEERSNTV*PH 308 + L+S++ RKP P P +G + + + F GT+L +K KL+F + S++ P Sbjct: 99 NQLLSRKKRKPTPPPSDGLYYVFRGKRIKKSFRPGTDLSTFKPKLLFADSAPSSSSDNPT 158 Query: 307 AAL-ISDQS 284 +++ ++D S Sbjct: 159 SSVDLNDYS 167 >SPAC20G8.01 |cdc17||ATP-dependent DNA ligase Cdc17|Schizosaccharomyces pombe|chr 1|||Manual Length = 768 Score = 25.8 bits (54), Expect = 5.9 Identities = 10/25 (40%), Positives = 16/25 (64%) Frame = +1 Query: 103 KDLMTTEDQMKFYECQLAYTQSQLE 177 +D T+E +FY Q+AY+Q + E Sbjct: 736 EDATTSEQVSEFYRSQVAYSQKEKE 760 >SPAC959.02 |sec17||alpha SNAP |Schizosaccharomyces pombe|chr 1|||Manual Length = 289 Score = 25.8 bits (54), Expect = 5.9 Identities = 14/29 (48%), Positives = 16/29 (55%), Gaps = 1/29 (3%) Frame = +3 Query: 444 GSGGFR-FLWDTKFDAAMVAFLDACNSSR 527 G+GGF F K+D A FLDA N R Sbjct: 18 GTGGFALFGGGNKYDEASELFLDAANGYR 46 >SPAC1687.22c |puf3|SPAC222.02c|RNA-binding protein Puf3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 732 Score = 25.8 bits (54), Expect = 5.9 Identities = 9/19 (47%), Positives = 14/19 (73%) Frame = +3 Query: 444 GSGGFRFLWDTKFDAAMVA 500 GSG F+ WD FD++++A Sbjct: 259 GSGSFKPSWDGSFDSSLMA 277 >SPCC417.06c |ppk35|mug27|serine/threonine protein kinase Ppk35|Schizosaccharomyces pombe|chr 3|||Manual Length = 624 Score = 25.8 bits (54), Expect = 5.9 Identities = 13/35 (37%), Positives = 22/35 (62%) Frame = -1 Query: 616 RMWWRCCILDFAFVYSVRKTKARITHFHLLLELLQ 512 R+W C + AF+ ++TK + +HF LL++L Q Sbjct: 137 RLWKEHCGRERAFLRK-KRTKIQCSHFDLLVKLGQ 170 >SPAC15A10.04c |zpr1||zinc finger protein Zpr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 459 Score = 25.4 bits (53), Expect = 7.9 Identities = 8/17 (47%), Positives = 12/17 (70%) Frame = -2 Query: 534 TCSLNCCRHLKMLPLPH 484 +CS C H+K+L +PH Sbjct: 261 SCSHQCDTHMKLLDIPH 277 >SPAC1F7.02c |||ATP-dependent RNA helicase Has1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 578 Score = 25.4 bits (53), Expect = 7.9 Identities = 12/38 (31%), Positives = 21/38 (55%) Frame = +1 Query: 88 YTKYRKDLMTTEDQMKFYECQLAYTQSQLEKLKKTNVF 201 + +Y K + ++ +F ++A QSQLEKL N + Sbjct: 448 FLRYLKTAKVSLNEFEFPANKVANVQSQLEKLVSKNYY 485 >SPBC11C11.09c |rpl502|rpl5-2, rpl5b|60S ribosomal protein L5|Schizosaccharomyces pombe|chr 2|||Manual Length = 294 Score = 25.4 bits (53), Expect = 7.9 Identities = 16/53 (30%), Positives = 25/53 (47%), Gaps = 3/53 (5%) Frame = +1 Query: 58 DKEQDVYWREYTKYRKD---LMTTEDQMKFYECQLAYTQSQLEKLKKTNVFKA 207 D+ +D+Y Y K R+D + +D F L YTQ +L ++ F A Sbjct: 234 DQLEDIYAEAYAKIREDPSFQKSGKDAAAFKAESLKYTQRKLTAEERKERFNA 286 >SPBC29A10.07 |||nucleoporin Pom152|Schizosaccharomyces pombe|chr 2|||Manual Length = 1250 Score = 25.4 bits (53), Expect = 7.9 Identities = 14/33 (42%), Positives = 21/33 (63%), Gaps = 1/33 (3%) Frame = -2 Query: 486 HRIWYPRETGNHPNR-ITVRLSGLPRLLCMSDS 391 +++ + +E N PN +TVR SG LL +SDS Sbjct: 786 NKVSHHKEVLNDPNSYLTVRKSGTYTLLSVSDS 818 >SPBC1604.18c |||vacuolar sorting protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 25.4 bits (53), Expect = 7.9 Identities = 13/35 (37%), Positives = 18/35 (51%) Frame = +1 Query: 220 SDSGQFGIINNFA*VACLLHRLIGRRLMRHGVKLY 324 S SGQ+ I N +ACL+H RR + +Y Sbjct: 150 SPSGQYVIRKNIEKLACLVHNEAMRRCSSYTSAIY 184 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,050,252 Number of Sequences: 5004 Number of extensions: 67999 Number of successful extensions: 197 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 191 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 196 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 321951680 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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