BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= prgv0664
(585 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1682.10 |rpn8||19S proteasome regulatory subunit Rpn8|Schizo... 101 1e-22
SPBC4C3.07 |||translation initiation factor eIF3f|Schizosaccharo... 33 0.041
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 25 6.2
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 25 6.2
SPCC1840.03 |sal3|pse1|karyopherin Sal3|Schizosaccharomyces pomb... 25 8.1
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 25 8.1
>SPCC1682.10 |rpn8||19S proteasome regulatory subunit
Rpn8|Schizosaccharomyces pombe|chr 3|||Manual
Length = 324
Score = 101 bits (241), Expect = 1e-22
Identities = 45/85 (52%), Positives = 59/85 (69%)
Frame = +1
Query: 10 HTGPKLHQNDIAINELIRRYCPNSVLVIIDAKPKDLGLPTEAYQAVEEVHDDGTPTSRTF 189
HTGP+L +D+ IN L+++Y PN VLVIID KPK +GLPT AY A++E+ DDG+ +SRTF
Sbjct: 103 HTGPQLRPSDLEINNLLKKYIPNPVLVIIDVKPKSVGLPTNAYFAIDEIEDDGSKSSRTF 162
Query: 190 EHVPSEIXXXXXXXXXXXHLLRISR 264
H+PS I HLLR +R
Sbjct: 163 VHLPSSIEAEEAEEIGVEHLLRDTR 187
Score = 75.4 bits (177), Expect = 6e-15
Identities = 34/70 (48%), Positives = 49/70 (70%)
Frame = +3
Query: 234 GSRAFVKDIKDTTVGSLSQRITNQLLGLKGLHSQLTEIRDYLVQVGQGSLPMNHQIIYQL 413
G ++D +D +VG+L+ R+T Q L+GL +LTEI DYL +V G LP+NH I+ +L
Sbjct: 178 GVEHLLRDTRDASVGTLATRVTQQAQSLQGLGQRLTEIADYLRKVVDGQLPINHAILAEL 237
Query: 414 QDIFNLLPDI 443
Q +FNLLP+I
Sbjct: 238 QSVFNLLPNI 247
>SPBC4C3.07 |||translation initiation factor
eIF3f|Schizosaccharomyces pombe|chr 2|||Manual
Length = 302
Score = 32.7 bits (71), Expect = 0.041
Identities = 26/95 (27%), Positives = 39/95 (41%), Gaps = 1/95 (1%)
Frame = +3
Query: 228 RSGSRAFVKDIKDTT-VGSLSQRITNQLLGLKGLHSQLTEIRDYLVQVGQGSLPMNHQII 404
RSG +A D + + SL + L S + + DY+ V GS P N +
Sbjct: 190 RSGLKAVAAPKNDPSRLASLFTDLQQLRRSTLELLSMIERVSDYVQNVIDGSSPANVAVG 249
Query: 405 YQLQDIFNLLPDIANDNFIDNLYVKTNDQSLVVYL 509
L F+L+P + +F D +VVYL
Sbjct: 250 RYLMKCFSLIPCVEGQDFEKIFSSHLQDVLVVVYL 284
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 25.4 bits (53), Expect = 6.2
Identities = 12/32 (37%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
Frame = +1
Query: 73 PNSVLVIIDAKPKDLGLPT-EAYQAVEEVHDD 165
P+ L + D+KP+ LG+ T + Y +V DD
Sbjct: 567 PDHTLRVKDSKPESLGIDTVKQYTGYLDVEDD 598
>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 3655
Score = 25.4 bits (53), Expect = 6.2
Identities = 14/34 (41%), Positives = 22/34 (64%)
Frame = +3
Query: 396 QIIYQLQDIFNLLPDIANDNFIDNLYVKTNDQSL 497
+I+ L D+F+LLP+ AN F+D L + + SL
Sbjct: 1459 KIVRALIDLFHLLPNNAN-RFMDELLICVVEFSL 1491
>SPCC1840.03 |sal3|pse1|karyopherin Sal3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1095
Score = 25.0 bits (52), Expect = 8.1
Identities = 12/46 (26%), Positives = 21/46 (45%)
Frame = +3
Query: 333 QLTEIRDYLVQVGQGSLPMNHQIIYQLQDIFNLLPDIANDNFIDNL 470
+++ R Y + + Q+I L + N+LP + D DNL
Sbjct: 193 RISAARAYSAVILESKQSTRDQVIPLLPSLMNILPPLQQDRDSDNL 238
>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1610
Score = 25.0 bits (52), Expect = 8.1
Identities = 11/29 (37%), Positives = 17/29 (58%)
Frame = +1
Query: 55 LIRRYCPNSVLVIIDAKPKDLGLPTEAYQ 141
LIR Y N + IID +D G+ E+++
Sbjct: 907 LIRIYLQNGIQSIIDVPIRDAGIFVESFE 935
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,177,846
Number of Sequences: 5004
Number of extensions: 40332
Number of successful extensions: 92
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 90
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 92
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 252150250
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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