BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0617 (697 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 126 2e-30 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 126 2e-30 SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 96 5e-21 SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 79 8e-16 SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 33 0.039 SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+, L-... 26 4.5 SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces pom... 26 4.5 SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein |Schizo... 26 5.9 SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb... 25 7.8 SPAC11D3.15 |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||M... 25 7.8 SPBC11B10.05c |rsp1||random septum position protein Rsp1|Schizos... 25 7.8 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 126 bits (305), Expect = 2e-30 Identities = 55/83 (66%), Positives = 64/83 (77%) Frame = +1 Query: 256 IQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQA 435 IQ E A+GGIY VLN++RGHVF E Q GTP++ +KAYLPVNESFGFT +LR T GQA Sbjct: 733 IQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYNIKAYLPVNESFGFTGELRQATAGQA 792 Query: 436 FPQCVFDHWQVLPGDPCEPQSKP 504 FPQ VFDHW + GDP +P SKP Sbjct: 793 FPQLVFDHWSPMSGDPLDPTSKP 815 Score = 124 bits (299), Expect = 1e-29 Identities = 59/86 (68%), Positives = 64/86 (74%) Frame = +2 Query: 8 SKGVQYLNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTT 187 +K V YLNEIKDSVVA F WA+KEG M EENLR RFNI DV LH DAIHRGGGQIIPT Sbjct: 650 TKAVAYLNEIKDSVVAAFAWASKEGPMFEENLRSCRFNILDVVLHADAIHRGGGQIIPTA 709 Query: 188 RRCLYACLLTAQPRLMEPVYLCEFSV 265 RR +YA L A P + EPV+L E V Sbjct: 710 RRVVYASTLLASPIIQEPVFLVEIQV 735 Score = 31.1 bits (67), Expect = 0.16 Identities = 16/34 (47%), Positives = 20/34 (58%) Frame = +3 Query: 456 PLAGPPWRPVRTSEQALHVVQETRKRKGLKEGLP 557 P++G P P Q +V E RKRKGLKE +P Sbjct: 803 PMSGDPLDPTSKPGQ---IVCEARKRKGLKENVP 833 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 126 bits (305), Expect = 2e-30 Identities = 55/83 (66%), Positives = 64/83 (77%) Frame = +1 Query: 256 IQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQA 435 IQ E A+GGIY VLN++RGHVF E Q GTP++ +KAYLPVNESFGFT +LR T GQA Sbjct: 733 IQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYNIKAYLPVNESFGFTGELRQATAGQA 792 Query: 436 FPQCVFDHWQVLPGDPCEPQSKP 504 FPQ VFDHW + GDP +P SKP Sbjct: 793 FPQLVFDHWSPMSGDPLDPTSKP 815 Score = 124 bits (299), Expect = 1e-29 Identities = 59/86 (68%), Positives = 64/86 (74%) Frame = +2 Query: 8 SKGVQYLNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTT 187 +K V YLNEIKDSVVA F WA+KEG M EENLR RFNI DV LH DAIHRGGGQIIPT Sbjct: 650 TKAVAYLNEIKDSVVAAFAWASKEGPMFEENLRSCRFNILDVVLHADAIHRGGGQIIPTA 709 Query: 188 RRCLYACLLTAQPRLMEPVYLCEFSV 265 RR +YA L A P + EPV+L E V Sbjct: 710 RRVVYASTLLASPIIQEPVFLVEIQV 735 Score = 31.1 bits (67), Expect = 0.16 Identities = 16/34 (47%), Positives = 20/34 (58%) Frame = +3 Query: 456 PLAGPPWRPVRTSEQALHVVQETRKRKGLKEGLP 557 P++G P P Q +V E RKRKGLKE +P Sbjct: 803 PMSGDPLDPTSKPGQ---IVCEARKRKGLKENVP 833 >SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 983 Score = 95.9 bits (228), Expect = 5e-21 Identities = 41/77 (53%), Positives = 53/77 (68%) Frame = +2 Query: 26 LNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYA 205 LN +K+ + GFQW +EG + +E +R V F + DV L + I+RGGGQIIPT RR Y+ Sbjct: 772 LNSVKEYIKQGFQWGTREGPLCDETIRNVNFRLMDVVLAPEQIYRGGGQIIPTARRVCYS 831 Query: 206 CLLTAQPRLMEPVYLCE 256 LTA PRLMEPVY+ E Sbjct: 832 SFLTASPRLMEPVYMVE 848 Score = 92.3 bits (219), Expect = 6e-20 Identities = 44/95 (46%), Positives = 61/95 (64%) Frame = +1 Query: 256 IQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQA 435 + P ++ IY +L RRRGHV ++ G+P+++V+A +PV +S GF DLR +T GQA Sbjct: 849 VHAPADSLPIIYDLLTRRRGHVLQDIPRPGSPLYLVRALIPVIDSCGFETDLRVHTQGQA 908 Query: 436 FPQCVFDHWQVLPGDPCEPQSKPYTLYRKRERGKD 540 Q VFDHWQV+PGDP + KP L + RG D Sbjct: 909 MCQMVFDHWQVVPGDPLDKSIKPKPL--EPARGSD 941 Score = 27.1 bits (57), Expect = 2.6 Identities = 15/49 (30%), Positives = 23/49 (46%) Frame = +3 Query: 402 CRFAFQHRRTGLPAVRIRPLAGPPWRPVRTSEQALHVVQETRKRKGLKE 548 C+ F H + + + P P R S+ A + +TR+RKGL E Sbjct: 910 CQMVFDHWQVVPGDPLDKSIKPKPLEPARGSDLARDFLIKTRRRKGLVE 958 >SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1000 Score = 78.6 bits (185), Expect = 8e-16 Identities = 35/82 (42%), Positives = 51/82 (62%) Frame = +1 Query: 238 ACISL*IQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRS 417 A S +Q +G +YGV+++RRG V +E GTP FIVKA +PV ESFGF ++ Sbjct: 865 AMYSCDVQATSEVLGRVYGVVSKRRGRVIDEEMKEGTPFFIVKALIPVVESFGFAVEILK 924 Query: 418 NTGGQAFPQCVFDHWQVLPGDP 483 T G A+PQ +F +++L +P Sbjct: 925 RTSGAAYPQLIFHGFEMLDENP 946 Score = 27.1 bits (57), Expect = 2.6 Identities = 20/85 (23%), Positives = 39/85 (45%), Gaps = 9/85 (10%) Frame = +2 Query: 29 NEIKDSVVAGFQWAAKEGVMAEENLRGV-----RFNIYDVTLHTDAIHRGG----GQIIP 181 +++ + VV FQ +G + E ++G+ +F+I D + + + GQ+I Sbjct: 786 SDLSEYVVTAFQLITHQGPLCAEPVQGICVSIDQFDISDDSEDSKLLTINNPQIPGQVIS 845 Query: 182 TTRRCLYACLLTAQPRLMEPVYLCE 256 + + L PRLM +Y C+ Sbjct: 846 VVKESIRHGFLGWSPRLMLAMYSCD 870 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 33.1 bits (72), Expect = 0.039 Identities = 16/59 (27%), Positives = 32/59 (54%) Frame = +1 Query: 256 IQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQ 432 I P GG+ G L++R+ + + F ++A +P+N F +++D+R+ T G+ Sbjct: 683 ITAPVEHQGGVIGNLDKRKATIVDSD--TDEDEFTLQAEVPLNSMFSYSSDIRALTKGK 739 >SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+, L-lysine forming] |Schizosaccharomyces pombe|chr 1|||Manual Length = 368 Score = 26.2 bits (55), Expect = 4.5 Identities = 14/40 (35%), Positives = 22/40 (55%) Frame = -2 Query: 186 VVGMIWPPPLWMASVWSVTS*MLNLTPRKFSSAITPSLAA 67 V G+ PPPL + S+ + + + + FS A+ PSL A Sbjct: 305 VKGVTTPPPLEVISIDHLPTLLPRESSEAFSEALIPSLLA 344 >SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces pombe|chr 1|||Manual Length = 166 Score = 26.2 bits (55), Expect = 4.5 Identities = 13/47 (27%), Positives = 23/47 (48%) Frame = -2 Query: 162 PLWMASVWSVTS*MLNLTPRKFSSAITPSLAAHWNPATTESLISLRY 22 P W+A V ++ S + P FSS + +L A+ N + + + Y Sbjct: 43 PFWLAEVLAINSFVSIHMPAPFSSVVRNALKANPNSVSIRDITTHYY 89 >SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 391 Score = 25.8 bits (54), Expect = 5.9 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = -1 Query: 184 CWNDLATTSMDGISMECYIIDVESNTTQI 98 CW++L+TTS + + II + TT I Sbjct: 211 CWDELSTTSPESSKVSEPIIQDNTQTTHI 239 >SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 2685 Score = 25.4 bits (53), Expect = 7.8 Identities = 15/53 (28%), Positives = 24/53 (45%) Frame = -1 Query: 295 HRRYHPQLLQDTEFTKIYRLHKTGLSS*QTCVQASSSCWNDLATTSMDGISME 137 H Y LL++ E I LHK+ + +Q S + + T + +SME Sbjct: 126 HAVYMSNLLRNVEQKWIQFLHKSWMKWLHISIQESQLTFPSVGTFELGSLSME 178 >SPAC11D3.15 |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1317 Score = 25.4 bits (53), Expect = 7.8 Identities = 11/31 (35%), Positives = 18/31 (58%) Frame = +2 Query: 23 YLNEIKDSVVAGFQWAAKEGVMAEENLRGVR 115 YL+ + +AGFQ G+ ++N +GVR Sbjct: 248 YLSPVVRRYLAGFQSGFLHGLKTKDNSKGVR 278 >SPBC11B10.05c |rsp1||random septum position protein Rsp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 494 Score = 25.4 bits (53), Expect = 7.8 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = +1 Query: 619 IYTESASRMFIFNNTCFRIILKQT 690 IYT+ A + IF N CF++ L T Sbjct: 424 IYTQYAFQWQIFKNKCFQLDLMNT 447 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,033,868 Number of Sequences: 5004 Number of extensions: 66509 Number of successful extensions: 163 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 147 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 163 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 321151040 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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