Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= prgv0617
(697 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 137 6e-33
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 97 1e-20
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 97 1e-20
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 95 5e-20
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 71 7e-13
At5g13650.2 68418.m01585 elongation factor family protein contai... 34 0.078
At5g13650.1 68418.m01584 elongation factor family protein contai... 34 0.078
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 34 0.078
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 34 0.078
At5g26920.1 68418.m03210 calmodulin-binding protein similar to c... 29 2.2
At1g65910.1 68414.m07479 no apical meristem (NAM) family protein... 29 2.9
At5g38660.1 68418.m04675 expressed protein similar to unknown pr... 29 3.9
At2g40070.1 68415.m04923 expressed protein 29 3.9
At1g34360.1 68414.m04266 translation initiation factor 3 (IF-3) ... 28 5.1
At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /... 27 9.0
At3g63180.1 68416.m07097 expressed protein 27 9.0
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 137 bits (332), Expect = 6e-33
Identities = 60/82 (73%), Positives = 72/82 (87%)
Frame = +2
Query: 11 KGVQYLNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTR 190
KGVQYLNEIKDSVVAGFQWA+KEG +AEEN+RG+ F + DV LH+DAIHRGGGQ+IPT R
Sbjct: 652 KGVQYLNEIKDSVVAGFQWASKEGPLAEENMRGICFEVCDVVLHSDAIHRGGGQVIPTAR 711
Query: 191 RCLYACLLTAQPRLMEPVYLCE 256
R +YA +TA+PRL+EPVY+ E
Sbjct: 712 RVIYASQITAKPRLLEPVYMVE 733
Score = 125 bits (302), Expect = 2e-29
Identities = 58/98 (59%), Positives = 74/98 (75%), Gaps = 3/98 (3%)
Frame = +1
Query: 256 IQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQA 435
IQ PE A+GGIY VLN++RGHVFEE Q GTP++ +KAYLPV ESFGF++ LR+ T GQA
Sbjct: 734 IQAPEGALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVVESFGFSSQLRAATSGQA 793
Query: 436 FPQCVFDHWQVLPGDPCEPQSKPYTL---YRKRERGKD 540
FPQCVFDHW+++ DP EP ++ L RKR+ K+
Sbjct: 794 FPQCVFDHWEMMSSDPLEPGTQASVLVADIRKRKGLKE 831
>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 97.1 bits (231), Expect = 1e-20
Identities = 46/78 (58%), Positives = 54/78 (69%)
Frame = +1
Query: 256 IQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQA 435
IQ P V IY VL+RRRGHV + GTP +IVKA+LPV ESFGF DLR +T GQA
Sbjct: 850 IQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQA 909
Query: 436 FPQCVFDHWQVLPGDPCE 489
F VFDHW ++PGDP +
Sbjct: 910 FCLSVFDHWAIVPGDPLD 927
Score = 92.3 bits (219), Expect = 3e-19
Identities = 39/74 (52%), Positives = 51/74 (68%)
Frame = +2
Query: 35 IKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLL 214
+KDS+V GFQW A+EG + +E +R V+F I D + + +HRG GQ+IPT RR Y+ L
Sbjct: 776 VKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFL 835
Query: 215 TAQPRLMEPVYLCE 256
A PRLMEPVY E
Sbjct: 836 MATPRLMEPVYYVE 849
>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 97.1 bits (231), Expect = 1e-20
Identities = 46/78 (58%), Positives = 54/78 (69%)
Frame = +1
Query: 256 IQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQA 435
IQ P V IY VL+RRRGHV + GTP +IVKA+LPV ESFGF DLR +T GQA
Sbjct: 850 IQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQA 909
Query: 436 FPQCVFDHWQVLPGDPCE 489
F VFDHW ++PGDP +
Sbjct: 910 FCLSVFDHWAIVPGDPLD 927
Score = 92.3 bits (219), Expect = 3e-19
Identities = 39/74 (52%), Positives = 51/74 (68%)
Frame = +2
Query: 35 IKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLL 214
+KDS+V GFQW A+EG + +E +R V+F I D + + +HRG GQ+IPT RR Y+ L
Sbjct: 776 VKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFL 835
Query: 215 TAQPRLMEPVYLCE 256
A PRLMEPVY E
Sbjct: 836 MATPRLMEPVYYVE 849
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 94.7 bits (225), Expect = 5e-20
Identities = 45/78 (57%), Positives = 54/78 (69%)
Frame = +1
Query: 256 IQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQA 435
IQ P V IY VL+RRRG+V + GTP +IVKA+LPV ESFGF DLR +T GQA
Sbjct: 836 IQTPIDCVTAIYTVLSRRRGYVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQA 895
Query: 436 FPQCVFDHWQVLPGDPCE 489
F VFDHW ++PGDP +
Sbjct: 896 FCLSVFDHWAIVPGDPLD 913
Score = 92.3 bits (219), Expect = 3e-19
Identities = 39/74 (52%), Positives = 51/74 (68%)
Frame = +2
Query: 35 IKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLL 214
+KDS+V GFQW A+EG + +E +R V+F I D + + +HRG GQ+IPT RR Y+ L
Sbjct: 762 VKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFL 821
Query: 215 TAQPRLMEPVYLCE 256
A PRLMEPVY E
Sbjct: 822 MATPRLMEPVYYVE 835
>At3g22980.1 68416.m02898 elongation factor Tu family protein similar
to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 70.9 bits (166), Expect = 7e-13
Identities = 31/69 (44%), Positives = 43/69 (62%)
Frame = +1
Query: 277 VGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFD 456
+G +Y VL+RRR + +E G+ +F V AY+PV+ESFGF +LR T G A V
Sbjct: 890 LGPMYAVLSRRRARILKEEMQEGSSLFTVHAYVPVSESFGFADELRKGTSGGASALMVLS 949
Query: 457 HWQVLPGDP 483
HW++L DP
Sbjct: 950 HWEMLEEDP 958
Score = 41.1 bits (92), Expect = 7e-04
Identities = 24/84 (28%), Positives = 41/84 (48%), Gaps = 8/84 (9%)
Frame = +2
Query: 35 IKDSVVAGFQWAAKEGVMAEENLRGVRFNIYD-----VTLHTDAIHRGG---GQIIPTTR 190
++ S+V+GFQ A G + +E + G+ F I + TD G GQ++ +
Sbjct: 801 LESSIVSGFQLATASGPLCDEPMWGLAFTIESHLAPAEDVETDKPENFGIFTGQVMTAVK 860
Query: 191 RCLYACLLTAQPRLMEPVYLCEFS 262
A +L PR++E +Y CE +
Sbjct: 861 DACRAAVLQTNPRIVEAMYFCELN 884
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 34.3 bits (75), Expect = 0.078
Identities = 18/75 (24%), Positives = 33/75 (44%)
Frame = +1
Query: 256 IQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQA 435
++ PE +G + +L +RRG +F+ V ++ +P G + + + G A
Sbjct: 483 VEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRNAILTASRGTA 542
Query: 436 FPQCVFDHWQVLPGD 480
VFD + GD
Sbjct: 543 ILNTVFDSYGPWAGD 557
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 34.3 bits (75), Expect = 0.078
Identities = 18/75 (24%), Positives = 33/75 (44%)
Frame = +1
Query: 256 IQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQA 435
++ PE +G + +L +RRG +F+ V ++ +P G + + + G A
Sbjct: 482 VEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRNAILTASRGTA 541
Query: 436 FPQCVFDHWQVLPGD 480
VFD + GD
Sbjct: 542 ILNTVFDSYGPWAGD 556
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 34.3 bits (75), Expect = 0.078
Identities = 17/59 (28%), Positives = 31/59 (52%)
Frame = +1
Query: 256 IQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQ 432
++ P G + G +N+R+G + Q ++ A +P+N FG++ LRS T G+
Sbjct: 666 LKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLNNMFGYSTSLRSMTQGK 722
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 34.3 bits (75), Expect = 0.078
Identities = 17/59 (28%), Positives = 31/59 (52%)
Frame = +1
Query: 256 IQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQ 432
++ P G + G +N+R+G + Q ++ A +P+N FG++ LRS T G+
Sbjct: 666 LKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLNNMFGYSTSLRSMTQGK 722
>At5g26920.1 68418.m03210 calmodulin-binding protein similar to
calmodulin-binding protein TCB60 GI:1698548 from
[Nicotiana tabacum]
Length = 492
Score = 29.5 bits (63), Expect = 2.2
Identities = 20/82 (24%), Positives = 37/82 (45%), Gaps = 2/82 (2%)
Frame = -1
Query: 328 LRKRDHDVCSVHRRYHPQLLQDTEFTKIYRLHKTGLSS*QTCVQASSSCWNDLATTSMDG 149
L K D S R ++L +F ++Y +++ L + S WN + + +MD
Sbjct: 175 LEKIAKDGVSATRLAERKILTVKDFRRLYTVNRNELHN-IIGAGVSKKTWNTIVSHAMDC 233
Query: 148 I--SMECYIIDVESNTTQILFS 89
+ ECYI + + +LF+
Sbjct: 234 VLDETECYIYNANTPGVTLLFN 255
>At1g65910.1 68414.m07479 no apical meristem (NAM) family protein
similar to jasmonic acid 2 GI:6175246 from [Lycopersicon
esculentum]; similar to NAC2 (GI:6456751) {Arabidopsis
thaliana}
Length = 631
Score = 29.1 bits (62), Expect = 2.9
Identities = 16/58 (27%), Positives = 29/58 (50%), Gaps = 2/58 (3%)
Frame = -2
Query: 531 SFSFPVQRVGLALRFARVSREDLPMVEYALREGLSAG--VGTQIGSKTERLIDR*VGL 364
+ S+P +V +AL AR+ LP V EGL+ G + + T+ ++ + + L
Sbjct: 249 TISYPPSKVDIALECARLQNRMLPPVPPLYVEGLTHNEYFGNNVANDTDEMLSKIIAL 306
>At5g38660.1 68418.m04675 expressed protein similar to unknown
protein (pir||S75762)
Length = 286
Score = 28.7 bits (61), Expect = 3.9
Identities = 15/56 (26%), Positives = 33/56 (58%), Gaps = 4/56 (7%)
Frame = -2
Query: 543 SILSSFSFPVQRVGLALRFARVSREDLPMVEYA----LREGLSAGVGTQIGSKTER 388
+I+ ++ FP+ +G+AL++A + + +P + Y+ LRE + + TQ+ + R
Sbjct: 126 AIMLTYGFPLSIIGMALKYAEL--KPVPCLSYSDAVKLRESCATPILTQVRNDVTR 179
>At2g40070.1 68415.m04923 expressed protein
Length = 607
Score = 28.7 bits (61), Expect = 3.9
Identities = 18/47 (38%), Positives = 22/47 (46%), Gaps = 1/47 (2%)
Frame = +2
Query: 419 TPADRPSRSAYSTIGRSSLETRANL-RASPTRCTGNEKEERIEGRSP 556
TP RP+ TI RSS TR + AS T N +I+ SP
Sbjct: 297 TPTSRPTLPPSKTISRSSTPTRRPIASASAATTTANPTISQIKPSSP 343
>At1g34360.1 68414.m04266 translation initiation factor 3 (IF-3)
family protein low similarity to Translation initiation
factor IF-3 from [subsp. Schizaphis graminum] {Buchnera
aphidicola} SP|P46243, {Salmonella typhimurium}
SP|P33321; contains Pfam profiles PF05198: Translation
initiation factor IF-3 N-terminal domain, PF00707:
Translation initiation factor IF-3 C-terminal domain
Length = 520
Score = 28.3 bits (60), Expect = 5.1
Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 1/51 (1%)
Frame = +2
Query: 488 NLRASPTRCTGNEKEERIEGRSPRLN-SIFGQIVNYIVKAN*KNVSIRKAL 637
NLR T +KE ++ PRLN I G V + + VS+R+AL
Sbjct: 62 NLRFLATSAQTRKKEAEVDSDGPRLNEKITGDYVRLVSEEGHCVVSLREAL 112
>At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein weak
similarity to polygalacturonase PG1 [Glycine max]
GI:5669846; contains PF00295: Glycosyl hydrolases family
28
Length = 444
Score = 27.5 bits (58), Expect = 9.0
Identities = 11/22 (50%), Positives = 17/22 (77%), Gaps = 1/22 (4%)
Frame = +3
Query: 309 SWSRFRRVPGGRY-TYVHCEGL 371
S+ R R +PGGRY +++H +GL
Sbjct: 123 SYGRGRELPGGRYMSFIHGDGL 144
>At3g63180.1 68416.m07097 expressed protein
Length = 978
Score = 27.5 bits (58), Expect = 9.0
Identities = 10/35 (28%), Positives = 21/35 (60%)
Frame = +2
Query: 368 PTYLSMSRSVLLPICVPTPADRPSRSAYSTIGRSS 472
P ++ + L P+ VP+ + RP++S + +G +S
Sbjct: 539 PAFIYPANHHLQPVMVPSKSSRPTKSPHLAVGLAS 573
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,075,404
Number of Sequences: 28952
Number of extensions: 359805
Number of successful extensions: 930
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 893
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 930
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1487069504
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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