Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= prgv0583
(676 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 61 2e-10
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 59 7e-10
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 51 1e-07
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 42 1e-04
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 37 0.003
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 32 0.066
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 27 1.9
SPBC16D10.06 |||ZIP zinc transporter 2|Schizosaccharomyces pombe... 27 1.9
SPAC1565.07c |||TATA binding protein interacting protein |Schizo... 27 2.5
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 27 3.3
SPBC119.14 |rti1||Rad22 homolog Rti1|Schizosaccharomyces pombe|c... 26 4.3
SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 26 4.3
SPBC23E6.07c |rfc1||DNA replication factor C complex subunit Rfc... 26 4.3
SPCC1322.01 ||SPCC23B6.06|3'-5' exonuclease for RNA 3' ss-tail|S... 25 7.6
SPAC3H1.12c |snt2||Lid2 complex subunit Snt2 |Schizosaccharomyce... 25 10.0
SPCC1795.01c |mad3|SPCC895.02|mitotic spindle checkpoint protein... 25 10.0
SPAC22E12.16c |pik1||phosphatidylinositol kinase Pik1|Schizosacc... 25 10.0
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 60.9 bits (141), Expect = 2e-10
Identities = 25/56 (44%), Positives = 41/56 (73%)
Frame = +2
Query: 341 DFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYEN 508
+F++G + FDKD G I ELR++L++LGEKLS++E+++LL+G G +NY +
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKDGMVNYHD 133
Score = 45.6 bits (103), Expect = 7e-06
Identities = 19/37 (51%), Positives = 26/37 (70%)
Frame = +1
Query: 127 FQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDV 237
+++AF LFD G G+I IGD LRA GQNPT +++
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI 44
Score = 27.5 bits (58), Expect = 1.9
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = +1
Query: 124 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDV 237
EF + FQ+FD G I V ++ L +LG+ + ++
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEM 115
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 58.8 bits (136), Expect = 7e-10
Identities = 27/60 (45%), Positives = 39/60 (65%), Gaps = 1/60 (1%)
Frame = +2
Query: 329 DTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLL-QGQEDSQGNINYE 505
D + E + FDKDGNG+I+ EL H+L++LGE+LS +EV ++ + D G INYE
Sbjct: 82 DNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYE 141
Score = 48.8 bits (111), Expect = 7e-07
Identities = 20/45 (44%), Positives = 34/45 (75%)
Frame = +1
Query: 106 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVK 240
+++Q+AEF+EAF LFD DG I ++G +R+LGQ+PT ++++
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQ 51
Score = 33.9 bits (74), Expect = 0.022
Identities = 18/64 (28%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
Frame = +2
Query: 341 DFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLL-QGQEDSQGNINYENLFT 517
+F E FD+D +G I+S EL ++ +LG+ + E++ ++ + D G I++ T
Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLT 72
Query: 518 SSCR 529
R
Sbjct: 73 MMAR 76
Score = 33.5 bits (73), Expect = 0.029
Identities = 15/42 (35%), Positives = 26/42 (61%)
Frame = +1
Query: 112 DQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDV 237
D E +EAF++FD G+G I V ++ L +LG+ ++ +V
Sbjct: 82 DNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEV 123
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 51.2 bits (117), Expect = 1e-07
Identities = 22/61 (36%), Positives = 40/61 (65%), Gaps = 1/61 (1%)
Frame = +2
Query: 323 QXDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQ-GQEDSQGNIN 499
+ ++ ++I+ R FDKD +G+I +A+ + TLGEKLSD+EV+ ++Q + G+ +
Sbjct: 73 ETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFD 132
Query: 500 Y 502
Y
Sbjct: 133 Y 133
Score = 41.9 bits (94), Expect = 8e-05
Identities = 20/51 (39%), Positives = 32/51 (62%)
Frame = +1
Query: 106 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHL 258
S++Q E +EAF L+D DG I + +G LR+LG N T++++ K + L
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL 54
Score = 31.9 bits (69), Expect = 0.087
Identities = 12/39 (30%), Positives = 25/39 (64%)
Frame = +1
Query: 124 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVK 240
E+ +AF++FD G I A+ D ++ LG+ ++++V+
Sbjct: 79 EYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQ 117
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 41.5 bits (93), Expect = 1e-04
Identities = 24/59 (40%), Positives = 34/59 (57%), Gaps = 1/59 (1%)
Frame = +1
Query: 106 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLNLM-KGYL 279
+E+Q + EAF+LFDS D I ++ A+RALG N +S+V K + KGYL
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYL 90
Score = 29.5 bits (63), Expect = 0.46
Identities = 13/43 (30%), Positives = 23/43 (53%)
Frame = +1
Query: 112 DQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVK 240
D L E + AF+LFD GKI + + + L +N + +++
Sbjct: 107 DPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELE 149
Score = 27.5 bits (58), Expect = 1.9
Identities = 17/56 (30%), Positives = 26/56 (46%)
Frame = +2
Query: 302 PGHIESTQXDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQ 469
P +E T+ + D E + FD D + I ELR + LG EV ++L+
Sbjct: 26 PLRVEITE-EQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILR 80
Score = 27.1 bits (57), Expect = 2.5
Identities = 15/60 (25%), Positives = 33/60 (55%), Gaps = 2/60 (3%)
Frame = +2
Query: 338 NDFIEGLRHFDKDGNGFISSAE-LRHLLSTLGEKLSDDEVEQLLQ-GQEDSQGNINYENL 511
++ ++ LR FDK G G++ + +R + + E+ +E+++ + +D G I+ NL
Sbjct: 73 SEVLKILRDFDKTGKGYLQMEDFVRVMTEKIVERDPLEEIKRAFELFDDDETGKISLRNL 132
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 36.7 bits (81), Expect = 0.003
Identities = 19/56 (33%), Positives = 30/56 (53%)
Frame = +2
Query: 338 NDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYE 505
ND +E FD +G I + +R LS++G+++ EVE +L+ S G YE
Sbjct: 117 NDLLEAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESILRSY-TSHGVFYYE 171
Score = 33.5 bits (73), Expect = 0.029
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = +1
Query: 97 AGYSEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDV 237
A + Q+ E +EAF L D GDG I + L +L Q+ +E +
Sbjct: 40 AQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSI 86
Score = 29.9 bits (64), Expect = 0.35
Identities = 11/40 (27%), Positives = 23/40 (57%)
Frame = +2
Query: 350 EGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQ 469
E DKDG+G I +++ +L++L + S+D + + +
Sbjct: 52 EAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFE 91
Score = 29.5 bits (63), Expect = 0.46
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +1
Query: 133 EAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVK 240
EAF FD GKI ++ + DAL ++G +V+
Sbjct: 121 EAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVE 156
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 32.3 bits (70), Expect = 0.066
Identities = 21/67 (31%), Positives = 34/67 (50%)
Frame = -1
Query: 457 LYLIITELLSESREQVSQFRRRDEPIAIFVKMAQTLNKVISSVALRAFDMAW*IGRNTSK 278
L+L+ L++ +Q + R + IAIF K + +K I SVA+ A + K
Sbjct: 1377 LHLLFASLVAHKFDQPQHAQTRTKIIAIFFKDLYSPHKEIYSVAIDALRHVLSQNQKLPK 1436
Query: 277 DILSSGL 257
++L SGL
Sbjct: 1437 ELLQSGL 1443
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 27.5 bits (58), Expect = 1.9
Identities = 13/49 (26%), Positives = 23/49 (46%)
Frame = +2
Query: 359 RHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYE 505
RHF+K + ++ E L++LG +E L +S+ + YE
Sbjct: 494 RHFEKKKSNMLNEVEFYAALASLGLVYDTEEGTALFHRAANSEEGVTYE 542
>SPBC16D10.06 |||ZIP zinc transporter 2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 408
Score = 27.5 bits (58), Expect = 1.9
Identities = 20/69 (28%), Positives = 32/69 (46%), Gaps = 1/69 (1%)
Frame = +2
Query: 287 VSANLPGHIESTQXDTANDFIEGLRHFDKD-GNGFISSAELRHLLSTLGEKLSDDEVEQL 463
+ NLP + + N +IE L F + G+G I + HLL+ KL D ++ L
Sbjct: 76 IGMNLPLVLSKITKNRPNVYIEYLYLFARYFGSGVILATAFIHLLAPACNKLYDPCLDDL 135
Query: 464 LQGQEDSQG 490
G + + G
Sbjct: 136 FGGYDWAPG 144
>SPAC1565.07c |||TATA binding protein interacting protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1220
Score = 27.1 bits (57), Expect = 2.5
Identities = 15/38 (39%), Positives = 24/38 (63%), Gaps = 2/38 (5%)
Frame = +2
Query: 359 RHFDKDGNGFISS-AELRHLLSTLGEK-LSDDEVEQLL 466
RH DK GN F ++ EL +L +G+K L++ +E +L
Sbjct: 670 RHIDKSGNEFTTAYLELLEVLLKVGQKYLAESLLEHIL 707
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 26.6 bits (56), Expect = 3.3
Identities = 14/47 (29%), Positives = 25/47 (53%)
Frame = +2
Query: 365 FDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYE 505
FD G+I +LR + LGE L+ +++ QL+ + G ++ E
Sbjct: 21 FDVTHKGYIDFEDLRRSCAQLGENLTKEQL-QLMLDLAGTNGKVSRE 66
Score = 25.8 bits (54), Expect = 5.7
Identities = 13/39 (33%), Positives = 20/39 (51%)
Frame = +1
Query: 124 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVK 240
E +EAF LFD G I + + LG+N T+ ++
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQ 51
>SPBC119.14 |rti1||Rad22 homolog Rti1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 371
Score = 26.2 bits (55), Expect = 4.3
Identities = 15/53 (28%), Positives = 24/53 (45%)
Frame = +2
Query: 371 KDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENLFTSSCR 529
K+G LR+ S++G L D Q + +Q NY+NL ++ R
Sbjct: 127 KEGTTDALKRALRNFGSSMGNCLYDKRYIQKILKMAPAQAEFNYDNLLRANKR 179
>SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1752
Score = 26.2 bits (55), Expect = 4.3
Identities = 16/64 (25%), Positives = 29/64 (45%), Gaps = 2/64 (3%)
Frame = -1
Query: 205 LLTHLRSERHVSFHLHEN--RRAGRLLEILPIDPLNIQPYYESTNEIEGNEIGIFKGCDL 32
+ TH+ SE L+E R ++ +LP+ P +++P G + K D+
Sbjct: 216 IFTHISSEDLAHLGLNEQYARPDWMIITVLPVPPPSVRPSISVDGTSRGEDDLTHKLSDI 275
Query: 31 SKSN 20
K+N
Sbjct: 276 IKAN 279
>SPBC23E6.07c |rfc1||DNA replication factor C complex subunit
Rfc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 934
Score = 26.2 bits (55), Expect = 4.3
Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
Frame = +2
Query: 428 GEKLSDDEVEQLLQG-QEDSQGNINYENLFTSSC 526
G KLS V+QL+QG Q D + IN + + SC
Sbjct: 558 GLKLSPQAVDQLVQGTQSDMRQIINLLSTYKLSC 591
>SPCC1322.01 ||SPCC23B6.06|3'-5' exonuclease for RNA 3'
ss-tail|Schizosaccharomyces pombe|chr 3|||Manual
Length = 957
Score = 25.4 bits (53), Expect = 7.6
Identities = 12/48 (25%), Positives = 23/48 (47%), Gaps = 2/48 (4%)
Frame = +3
Query: 159 WRWKDTCRS--DRRCVKSFRTKSYRVRCEKMYSSLKPDERISFEVFLP 296
W + + S + +K FRT +RC ++ LK ++ +V +P
Sbjct: 191 WNYSNVLTSINENGTIKEFRTSRVLLRCPNVFKKLKEVQQPLSDVSVP 238
>SPAC3H1.12c |snt2||Lid2 complex subunit Snt2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1131
Score = 25.0 bits (52), Expect = 10.0
Identities = 8/14 (57%), Positives = 10/14 (71%)
Frame = +1
Query: 382 WVHLFCGTATPALY 423
WVHL C + TP +Y
Sbjct: 927 WVHLICASWTPDVY 940
>SPCC1795.01c |mad3|SPCC895.02|mitotic spindle checkpoint protein
Mad3|Schizosaccharomyces pombe|chr 3|||Manual
Length = 310
Score = 25.0 bits (52), Expect = 10.0
Identities = 10/33 (30%), Positives = 20/33 (60%)
Frame = -3
Query: 290 KHLKRYPFIRFK*RVHFFTSDSVGFCPKALNAS 192
K +K PF+RF+ + FT + F P++ +++
Sbjct: 177 KRMKAKPFLRFQQKYQQFTHRWLEFAPQSFSSN 209
>SPAC22E12.16c |pik1||phosphatidylinositol kinase
Pik1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 851
Score = 25.0 bits (52), Expect = 10.0
Identities = 12/37 (32%), Positives = 20/37 (54%)
Frame = +2
Query: 296 NLPGHIESTQXDTANDFIEGLRHFDKDGNGFISSAEL 406
N+ + S+ DT + G RH D DGNG + +++
Sbjct: 427 NVSLQVPSSHRDTDVVLLSG-RHSDSDGNGALKRSKI 462
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,504,442
Number of Sequences: 5004
Number of extensions: 48518
Number of successful extensions: 192
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 174
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 192
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 309878492
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -