Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= prgv0566
(616 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g12780.1 68416.m01596 phosphoglycerate kinase, putative simil... 74 7e-14
At1g56190.1 68414.m06458 phosphoglycerate kinase, putative simil... 74 9e-14
At1g79550.2 68414.m09274 phosphoglycerate kinase, putative simil... 73 2e-13
At1g79550.1 68414.m09273 phosphoglycerate kinase, putative simil... 73 2e-13
At1g34590.1 68414.m04299 hypothetical protein 32 0.35
At5g18200.1 68418.m02136 expressed protein 31 0.61
At2g33200.1 68415.m04067 F-box family protein contains F-box dom... 30 1.1
At4g01880.1 68417.m00246 expressed protein contains Pfam PF05206... 29 3.2
At2g32300.1 68415.m03949 uclacyanin I identical to uclacyanin I ... 29 3.2
At1g21590.1 68414.m02699 protein kinase family protein contains ... 29 3.2
At2g47250.1 68415.m05900 RNA helicase, putative similar to SP|P5... 28 4.3
At3g03850.1 68416.m00396 auxin-responsive protein, putative simi... 28 5.7
At5g58360.1 68418.m07307 ovate family protein 69% similar to ova... 27 7.5
At5g49240.1 68418.m06095 two-component responsive regulator fami... 27 9.9
At3g62310.1 68416.m07000 RNA helicase, putative similar to SP|P5... 27 9.9
At3g31540.1 68416.m04025 hypothetical protein 27 9.9
>At3g12780.1 68416.m01596 phosphoglycerate kinase, putative similar
to SP|P41758 Phosphoglycerate kinase, chloroplast
precursor (EC 2.7.2.3) {Chlamydomonas reinhardtii};
contains Pfam profile PF00162: phosphoglycerate kinase
Length = 481
Score = 74.1 bits (174), Expect = 7e-14
Identities = 36/71 (50%), Positives = 50/71 (70%)
Frame = +1
Query: 256 VVLMSHLGRPDGQVNLKYTLKPVAEELKKLLNKDVTFLNDCIGPEVETACANPSAGSIIL 435
V+L +HLGRP G V K++L P+ L +LL +VT +DCIGPEVE+ A+ G ++L
Sbjct: 134 VILSTHLGRPKG-VTPKFSLAPLVPRLSELLGIEVTKADDCIGPEVESLVASLPEGGVLL 192
Query: 436 LENLRFHIEEE 468
LEN+RF+ EEE
Sbjct: 193 LENVRFYKEEE 203
Score = 46.0 bits (104), Expect = 2e-05
Identities = 21/49 (42%), Positives = 34/49 (69%), Gaps = 1/49 (2%)
Frame = +2
Query: 110 IDALNLTGKRVLMRVDFNVPLKEG-VITNNQRIVAALDSVKYALDKGAK 253
+ + +L GK+V +R D NVPL + IT++ RI AA+ ++KY ++ GAK
Sbjct: 85 LTSADLKGKKVFVRADLNVPLDDNQTITDDTRIRAAIPTIKYLIENGAK 133
Score = 43.6 bits (98), Expect = 1e-04
Identities = 18/29 (62%), Positives = 21/29 (72%)
Frame = +3
Query: 528 FRASLRKLGDVYINDAFGTAHRAHSSMVG 614
F L L D+Y+NDAFGTAHRAH+S G
Sbjct: 209 FAKKLASLADLYVNDAFGTAHRAHASTEG 237
>At1g56190.1 68414.m06458 phosphoglycerate kinase, putative similar
to SP|P41758 Phosphoglycerate kinase, chloroplast
precursor (EC 2.7.2.3) {Chlamydomonas reinhardtii};
contains Pfam profile PF00162: phosphoglycerate kinase
Length = 478
Score = 73.7 bits (173), Expect = 9e-14
Identities = 36/71 (50%), Positives = 49/71 (69%)
Frame = +1
Query: 256 VVLMSHLGRPDGQVNLKYTLKPVAEELKKLLNKDVTFLNDCIGPEVETACANPSAGSIIL 435
V+L +HLGRP G V K++L P+ L +LL +V +DCIGPEVET A+ G ++L
Sbjct: 131 VILSTHLGRPKG-VTPKFSLAPLVPRLSELLGIEVVKADDCIGPEVETLVASLPEGGVLL 189
Query: 436 LENLRFHIEEE 468
LEN+RF+ EEE
Sbjct: 190 LENVRFYKEEE 200
Score = 45.2 bits (102), Expect = 3e-05
Identities = 20/49 (40%), Positives = 36/49 (73%), Gaps = 1/49 (2%)
Frame = +2
Query: 110 IDALNLTGKRVLMRVDFNVPLKEGV-ITNNQRIVAALDSVKYALDKGAK 253
+++++L GK+V +R D NVPL + IT++ RI AA+ ++K+ ++ GAK
Sbjct: 82 LNSVDLKGKKVFVRADLNVPLDDNQNITDDTRIRAAIPTIKFLIENGAK 130
Score = 43.6 bits (98), Expect = 1e-04
Identities = 18/29 (62%), Positives = 21/29 (72%)
Frame = +3
Query: 528 FRASLRKLGDVYINDAFGTAHRAHSSMVG 614
F L L D+Y+NDAFGTAHRAH+S G
Sbjct: 206 FAKKLASLADLYVNDAFGTAHRAHASTEG 234
>At1g79550.2 68414.m09274 phosphoglycerate kinase, putative similar
to SP|P41758 Phosphoglycerate kinase, chloroplast
precursor (EC 2.7.2.3) {Chlamydomonas reinhardtii};
contains Pfam profile PF00162: phosphoglycerate kinase
Length = 401
Score = 72.5 bits (170), Expect = 2e-13
Identities = 37/71 (52%), Positives = 46/71 (64%)
Frame = +1
Query: 256 VVLMSHLGRPDGQVNLKYTLKPVAEELKKLLNKDVTFLNDCIGPEVETACANPSAGSIIL 435
VVL SHLGRP G V KY+LKP+ L +LL +V ND IG EV+ A G ++L
Sbjct: 59 VVLCSHLGRPKG-VTPKYSLKPLVPRLSELLGVEVVMANDSIGEEVQKLVAGLPEGGVLL 117
Query: 436 LENLRFHIEEE 468
LEN+RF+ EEE
Sbjct: 118 LENVRFYAEEE 128
Score = 44.4 bits (100), Expect = 6e-05
Identities = 19/29 (65%), Positives = 21/29 (72%)
Frame = +3
Query: 528 FRASLRKLGDVYINDAFGTAHRAHSSMVG 614
F L L DVY+NDAFGTAHRAH+S G
Sbjct: 134 FAKKLAALADVYVNDAFGTAHRAHASTEG 162
Score = 41.9 bits (94), Expect = 3e-04
Identities = 22/50 (44%), Positives = 34/50 (68%), Gaps = 1/50 (2%)
Frame = +2
Query: 122 NLTGKRVLMRVDFNVPLKEGV-ITNNQRIVAALDSVKYALDKGAK*LYLC 268
+L GK V +RVD NVPL + IT++ RI AA+ ++KY + G++ + LC
Sbjct: 14 DLKGKSVFVRVDLNVPLDDNSNITDDTRIRAAVPTIKYLMGNGSR-VVLC 62
>At1g79550.1 68414.m09273 phosphoglycerate kinase, putative similar
to SP|P41758 Phosphoglycerate kinase, chloroplast
precursor (EC 2.7.2.3) {Chlamydomonas reinhardtii};
contains Pfam profile PF00162: phosphoglycerate kinase
Length = 401
Score = 72.5 bits (170), Expect = 2e-13
Identities = 37/71 (52%), Positives = 46/71 (64%)
Frame = +1
Query: 256 VVLMSHLGRPDGQVNLKYTLKPVAEELKKLLNKDVTFLNDCIGPEVETACANPSAGSIIL 435
VVL SHLGRP G V KY+LKP+ L +LL +V ND IG EV+ A G ++L
Sbjct: 59 VVLCSHLGRPKG-VTPKYSLKPLVPRLSELLGVEVVMANDSIGEEVQKLVAGLPEGGVLL 117
Query: 436 LENLRFHIEEE 468
LEN+RF+ EEE
Sbjct: 118 LENVRFYAEEE 128
Score = 44.4 bits (100), Expect = 6e-05
Identities = 19/29 (65%), Positives = 21/29 (72%)
Frame = +3
Query: 528 FRASLRKLGDVYINDAFGTAHRAHSSMVG 614
F L L DVY+NDAFGTAHRAH+S G
Sbjct: 134 FAKKLAALADVYVNDAFGTAHRAHASTEG 162
Score = 41.9 bits (94), Expect = 3e-04
Identities = 22/50 (44%), Positives = 34/50 (68%), Gaps = 1/50 (2%)
Frame = +2
Query: 122 NLTGKRVLMRVDFNVPLKEGV-ITNNQRIVAALDSVKYALDKGAK*LYLC 268
+L GK V +RVD NVPL + IT++ RI AA+ ++KY + G++ + LC
Sbjct: 14 DLKGKSVFVRVDLNVPLDDNSNITDDTRIRAAVPTIKYLMGNGSR-VVLC 62
>At1g34590.1 68414.m04299 hypothetical protein
Length = 820
Score = 31.9 bits (69), Expect = 0.35
Identities = 17/52 (32%), Positives = 25/52 (48%)
Frame = -3
Query: 455 WKRRFSSRIIEPAEGLAHAVSTSGPMQSFKNVTSLFSNFFSSSATGLRVYLR 300
W + FS +E A L H VS ++SF + T F + S+ T VY +
Sbjct: 349 WLKHFSRERVERALRLLHGVSCPTSLESFDHRTQFFVDMQSTKLTLWEVYAK 400
>At5g18200.1 68418.m02136 expressed protein
Length = 351
Score = 31.1 bits (67), Expect = 0.61
Identities = 17/50 (34%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Frame = -2
Query: 312 GVLEIYLAIRSTQMRHK-YNYLAPLSKAYLTESKAATMRWLFVITPSLSG 166
G+L++ L + Q+ YNY+ S +TES+ W I P LSG
Sbjct: 272 GLLKLMLQKIAKQLNDPPYNYMIHTSPLKVTESQLPYTHWFLQIVPQLSG 321
>At2g33200.1 68415.m04067 F-box family protein contains F-box domain
Pfam:PF00646
Length = 376
Score = 30.3 bits (65), Expect = 1.1
Identities = 18/67 (26%), Positives = 30/67 (44%)
Frame = -3
Query: 503 FTLAPEASTPLPSSSMWKRRFSSRIIEPAEGLAHAVSTSGPMQSFKNVTSLFSNFFSSSA 324
+T + PL + + S+ + +P E H + G S +NV + SN+F
Sbjct: 39 YTASTTCKRPLYPWRIKFNKISTSLFDPREDKIHEIQHPGIEFSDRNVLASCSNWFLMVD 98
Query: 323 TGLRVYL 303
+GL YL
Sbjct: 99 SGLEFYL 105
>At4g01880.1 68417.m00246 expressed protein contains Pfam PF05206:
Protein of unknown function (DUF715)
Length = 453
Score = 28.7 bits (61), Expect = 3.2
Identities = 18/43 (41%), Positives = 25/43 (58%)
Frame = +1
Query: 412 PSAGSIILLENLRFHIEEEGKGVDASGAKVKAVQKKLKPLGQA 540
P I LL++ EEEG+ DAS V+ V KK+KP+ +A
Sbjct: 360 PGVDDIDLLDS-NAKEEEEGEVRDASLISVEEVVKKMKPMERA 401
>At2g32300.1 68415.m03949 uclacyanin I identical to uclacyanin I
GI:3399767 from [Arabidopsis thaliana]; contains Pfam
profile PF02298: Plastocyanin-like domain; identical to
cDNA uclacyanin I GI:3399766
Length = 261
Score = 28.7 bits (61), Expect = 3.2
Identities = 21/62 (33%), Positives = 29/62 (46%), Gaps = 2/62 (3%)
Frame = -3
Query: 497 LAPEASTPLPSSSMWKRRFSSRIIEP--AEGLAHAVSTSGPMQSFKNVTSLFSNFFSSSA 324
L+P +STPLPSSS+ S + P A G + + P S ++ F SS
Sbjct: 163 LSPSSSTPLPSSSLPLIPPLSPALSPATAAGTSLPLFPGSPGSSSSTTSTKTVGTFPSST 222
Query: 323 TG 318
TG
Sbjct: 223 TG 224
>At1g21590.1 68414.m02699 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 756
Score = 28.7 bits (61), Expect = 3.2
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = -3
Query: 440 SSRIIEPAEGLAHAVSTSGPMQSFKNVTSLFSNFFSSSATG 318
S R E EGL +STS ++K + S+ SNF + + G
Sbjct: 376 SGRFPENVEGLQARISTSCQFFTYKELVSVTSNFCADNFIG 416
>At2g47250.1 68415.m05900 RNA helicase, putative similar to
SP|P53131 Pre-mRNA splicing factor RNA helicase PRP43
(Helicase JA1) {Saccharomyces cerevisiae}; contains Pfam
profiles PF04408: Helicase associated domain (HA2),
PF00271: Helicase conserved C-terminal domain
Length = 729
Score = 28.3 bits (60), Expect = 4.3
Identities = 13/43 (30%), Positives = 24/43 (55%)
Frame = +1
Query: 259 VLMSHLGRPDGQVNLKYTLKPVAEELKKLLNKDVTFLNDCIGP 387
V+ H+ P G + + T + E+ + +NK+V+ L D +GP
Sbjct: 270 VVQIHMCEPPGDILVFLTGEEEIEDACRKINKEVSNLGDQVGP 312
>At3g03850.1 68416.m00396 auxin-responsive protein, putative similar
to small auxin-up regulated protein SAUR (GI:3043536)
[Raphanus sativus]
Length = 93
Score = 27.9 bits (59), Expect = 5.7
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = -3
Query: 599 VRSMCSTKSIINIDITKFSQACPKGF 522
VRS+ S K I+ + K S+A PKGF
Sbjct: 4 VRSLFSAKKILGGSLVKTSKAPPKGF 29
>At5g58360.1 68418.m07307 ovate family protein 69% similar to ovate
protein (GI:23429649) [Lycopersicon esculentum];
contains TIGRFAM TIGR01568 : uncharacterized
plant-specific domain TIGR01568
Length = 296
Score = 27.5 bits (58), Expect = 7.5
Identities = 19/59 (32%), Positives = 33/59 (55%), Gaps = 1/59 (1%)
Frame = -3
Query: 491 PEASTPLPSSSMWKRRFSSR-IIEPAEGLAHAVSTSGPMQSFKNVTSLFSNFFSSSATG 318
P+AS P SS +KR+ + + +P+ L +STS + + ++ +S +N S SA G
Sbjct: 67 PQASNSPPKSSSFKRKIKRKTVYKPSSRL--KLSTSSSL-NHRSKSSSSANAISDSAVG 122
>At5g49240.1 68418.m06095 two-component responsive regulator family
protein / response regulator family protein contains
Pfam profile: PF00072 response regulator receiver
domain; contains similarity to two-component response
regulator proteins
Length = 292
Score = 27.1 bits (57), Expect = 9.9
Identities = 19/48 (39%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
Frame = -3
Query: 419 AEGLAH-AVSTSGPMQSFKNVTSLFSNFFSSSATGLRVYLRFTWPSGL 279
AE L H V T G F+N+T+ + F S+ GLRV + PS L
Sbjct: 7 AEILDHRGVLTDGDDGPFRNLTNFYDMFSSNFPEGLRVLVFDEDPSYL 54
>At3g62310.1 68416.m07000 RNA helicase, putative similar to
SP|P53131 Pre-mRNA splicing factor RNA helicase PRP43
(Helicase JA1) {Saccharomyces cerevisiae}; contains Pfam
profiles PF04408: Helicase associated domain (HA2),
PF00271: Helicase conserved C-terminal domain
Length = 726
Score = 27.1 bits (57), Expect = 9.9
Identities = 13/43 (30%), Positives = 23/43 (53%)
Frame = +1
Query: 259 VLMSHLGRPDGQVNLKYTLKPVAEELKKLLNKDVTFLNDCIGP 387
V+ H+ P G + + T + E+ + +NK+V L D +GP
Sbjct: 266 VVQIHMCEPPGDILVFLTGEEEIEDACRKINKEVGNLGDQVGP 308
>At3g31540.1 68416.m04025 hypothetical protein
Length = 699
Score = 27.1 bits (57), Expect = 9.9
Identities = 16/50 (32%), Positives = 22/50 (44%)
Frame = -3
Query: 455 WKRRFSSRIIEPAEGLAHAVSTSGPMQSFKNVTSLFSNFFSSSATGLRVY 306
W + FS +E A L H VS +S + T F + +S T VY
Sbjct: 323 WTKHFSRDRVERALKLFHGVSCPSSSESSDHRTQFFVDMQTSKPTIREVY 372
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,885,291
Number of Sequences: 28952
Number of extensions: 220499
Number of successful extensions: 588
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 570
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 583
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1236350304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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