BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0545 (576 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g02590.1 68416.m00250 delta 7-sterol-C5-desaturase, putative ... 29 2.9 At5g37400.1 68418.m04499 hypothetical protein contains Pfam PF04... 28 3.9 At3g02580.1 68416.m00249 delta 7-sterol-C5-desaturase (STE1) ide... 28 3.9 >At3g02590.1 68416.m00250 delta 7-sterol-C5-desaturase, putative similar to delta7 sterol C-5 desaturase GI:5031219 from [Arabidopsis thaliana] Length = 279 Score = 28.7 bits (61), Expect = 2.9 Identities = 13/48 (27%), Positives = 24/48 (50%) Frame = +2 Query: 266 NRSKLKYIIF*SCWRRRPNFISTFILIFWTS*SLYFNFTRIWYNFSYY 409 NR L +++ + W P+F+ T++ + LYF +W + YY Sbjct: 20 NRMVLSHLLPVNLWEPLPHFLQTWLRNYLAGNILYFISGFLWCFYIYY 67 >At5g37400.1 68418.m04499 hypothetical protein contains Pfam PF04510 : Family of unknown function (DUF577)); common family comprised of At5g37410, At5g37400, At5g37920, At5g37460, At5g37650, At5g37470, At5g37420, At5g37430 Length = 623 Score = 28.3 bits (60), Expect = 3.9 Identities = 16/62 (25%), Positives = 38/62 (61%), Gaps = 1/62 (1%) Frame = -1 Query: 426 FLFLVK*YEKLYQILVK-LKYKLQDVQKIKINVDIKLGLLLQQDQKMMYLSFDLLIILYS 250 + FL +++Y+IL++ ++Y+++ V + + +K+G+LL + L+ D+L I++ Sbjct: 180 YRFLQNFLDEVYKILLRPVEYEVE-VWILALKSAVKMGILLMDSEMRFDLTRDILHIVWK 238 Query: 249 SS 244 S Sbjct: 239 CS 240 >At3g02580.1 68416.m00249 delta 7-sterol-C5-desaturase (STE1) identical to sterol-C5-desaturase GB:AAD12944 GI:4234768 from [Arabidopsis thaliana] Length = 281 Score = 28.3 bits (60), Expect = 3.9 Identities = 13/48 (27%), Positives = 24/48 (50%) Frame = +2 Query: 266 NRSKLKYIIF*SCWRRRPNFISTFILIFWTS*SLYFNFTRIWYNFSYY 409 NR L +++ + W P+F+ T++ + LYF +W + YY Sbjct: 19 NRIVLSHLLPANLWEPLPHFLQTWLRNYLAGTLLYFISGFLWCFYIYY 66 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,846,246 Number of Sequences: 28952 Number of extensions: 168870 Number of successful extensions: 268 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 265 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 268 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1121903184 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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