Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= prgv0538
(615 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 157 2e-39
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 157 2e-39
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 157 2e-39
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 45 8e-06
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 32 0.076
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 31 0.13
SPAC23A1.04c |mnl1||alpha mannosidase-like protein|Schizosacchar... 29 0.40
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 29 0.71
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 2.8
SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA syntheta... 26 5.0
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 157 bits (380), Expect = 2e-39
Identities = 69/85 (81%), Positives = 76/85 (89%)
Frame = +2
Query: 2 KSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNH 181
KSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNH
Sbjct: 288 KSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNH 347
Query: 182 PGQISNGYTPVLDCHTAHIACKFAE 256
PGQIS GY+PVLDCHTAHIACKFAE
Sbjct: 348 PGQISAGYSPVLDCHTAHIACKFAE 372
Score = 119 bits (286), Expect = 4e-28
Identities = 58/95 (61%), Positives = 69/95 (72%), Gaps = 1/95 (1%)
Frame = +1
Query: 226 HCPH-CLQICRIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGR 402
H H + + EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGR
Sbjct: 362 HTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGR 421
Query: 403 FAVRDMRQTVAVGVIKAVNFKEAGGGKVTKAAEKA 507
FAVRDMRQTVAVGVIKAV G KVTKAA KA
Sbjct: 422 FAVRDMRQTVAVGVIKAVEKVAPGAAKVTKAAVKA 456
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 157 bits (380), Expect = 2e-39
Identities = 69/85 (81%), Positives = 76/85 (89%)
Frame = +2
Query: 2 KSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNH 181
KSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNH
Sbjct: 288 KSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNH 347
Query: 182 PGQISNGYTPVLDCHTAHIACKFAE 256
PGQIS GY+PVLDCHTAHIACKFAE
Sbjct: 348 PGQISAGYSPVLDCHTAHIACKFAE 372
Score = 119 bits (286), Expect = 4e-28
Identities = 58/95 (61%), Positives = 69/95 (72%), Gaps = 1/95 (1%)
Frame = +1
Query: 226 HCPH-CLQICRIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGR 402
H H + + EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGR
Sbjct: 362 HTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGR 421
Query: 403 FAVRDMRQTVAVGVIKAVNFKEAGGGKVTKAAEKA 507
FAVRDMRQTVAVGVIKAV G KVTKAA KA
Sbjct: 422 FAVRDMRQTVAVGVIKAVEKVAPGAAKVTKAAVKA 456
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 157 bits (380), Expect = 2e-39
Identities = 69/85 (81%), Positives = 76/85 (89%)
Frame = +2
Query: 2 KSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNH 181
KSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNH
Sbjct: 288 KSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNH 347
Query: 182 PGQISNGYTPVLDCHTAHIACKFAE 256
PGQIS GY+PVLDCHTAHIACKFAE
Sbjct: 348 PGQISAGYSPVLDCHTAHIACKFAE 372
Score = 119 bits (286), Expect = 4e-28
Identities = 58/95 (61%), Positives = 69/95 (72%), Gaps = 1/95 (1%)
Frame = +1
Query: 226 HCPH-CLQICRIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGR 402
H H + + EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGR
Sbjct: 362 HTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGR 421
Query: 403 FAVRDMRQTVAVGVIKAVNFKEAGGGKVTKAAEKA 507
FAVRDMRQTVAVGVIKAV G KVTKAA KA
Sbjct: 422 FAVRDMRQTVAVGVIKAVEKVAPGAAKVTKAAVKA 456
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 45.2 bits (102), Expect = 8e-06
Identities = 23/74 (31%), Positives = 41/74 (55%), Gaps = 1/74 (1%)
Frame = +1
Query: 241 LQICRIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDM 420
+ ++ K+D+ T + ++ P G I L P+C+E F+++ +GRF +RD
Sbjct: 590 VSFAKLLHKLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQ 648
Query: 421 RQTVAVG-VIKAVN 459
TVAVG V+K ++
Sbjct: 649 GTTVAVGKVVKILD 662
Score = 42.3 bits (95), Expect = 5e-05
Identities = 24/78 (30%), Positives = 39/78 (50%)
Frame = +2
Query: 23 EALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNG 202
E + ++ GD V V+ +++ GYV +KN P F AQ+ +L P ++ G
Sbjct: 519 EEISSSICGDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTG 576
Query: 203 YTPVLDCHTAHIACKFAE 256
Y+ V+ HTA FA+
Sbjct: 577 YSCVMHIHTAVEEVSFAK 594
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 31.9 bits (69), Expect = 0.076
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = +1
Query: 358 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 450
PLC+ +E P LGRF +R TVA G++K
Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 31.1 bits (67), Expect = 0.13
Identities = 12/34 (35%), Positives = 22/34 (64%)
Frame = +2
Query: 8 VEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYV 109
+EM + L AV GDN G ++++ ++L+RG +
Sbjct: 301 IEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMI 334
>SPAC23A1.04c |mnl1||alpha mannosidase-like
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 787
Score = 29.5 bits (63), Expect = 0.40
Identities = 12/47 (25%), Positives = 25/47 (53%)
Frame = -1
Query: 345 QVDNGCISRLDGFWVDFSRFTSTTVNFFFDSANLQAMWAVWQSKTGV 205
+V C + ++FS T+ T N F ++ A ++VW+ ++G+
Sbjct: 198 EVTENCAAAASSLVLEFSMLTALTGNNKFKASAENAFFSVWKRRSGI 244
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 28.7 bits (61), Expect = 0.71
Identities = 18/53 (33%), Positives = 30/53 (56%)
Frame = -2
Query: 335 MAASPDLMDFGLTSVDLPVRRSTFSLILQICRQCGQCGNPRLVCNRLRFDQDG 177
++ +PDL D L+SVD P++ +T C + GN L+C+ F++DG
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNT----------CSESGNQYLLCD---FNRDG 66
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 2.8
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -3
Query: 94 QFLDGHVLYVETYIVSRYSFLESFV 20
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA synthetases
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 450
Score = 25.8 bits (54), Expect = 5.0
Identities = 11/43 (25%), Positives = 19/43 (44%)
Frame = -2
Query: 323 PDLMDFGLTSVDLPVRRSTFSLILQICRQCGQCGNPRLVCNRL 195
P ++DF + ++ V+ + CG PR VC+ L
Sbjct: 279 PSMIDFRIGFIEKAVKHPNADSLYVSTIHCGDAEGPRTVCSGL 321
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,645,419
Number of Sequences: 5004
Number of extensions: 52378
Number of successful extensions: 130
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 121
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 130
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 269634532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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