Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= prgv0519
(608 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q4TI68 Cluster: Chromosome undetermined SCAF2366, whole... 65 1e-09
UniRef50_O14735 Cluster: CDP-diacylglycerol--inositol 3-phosphat... 64 2e-09
UniRef50_UPI000065FDB0 Cluster: CDP-diacylglycerol--inositol 3-p... 63 4e-09
UniRef50_Q6NYP5 Cluster: CDP-diacylglycerol--inositol 3-phosphat... 62 1e-08
UniRef50_Q6BUR5 Cluster: Similar to sp|P06197 Saccharomyces cere... 55 1e-06
UniRef50_A1DBH6 Cluster: Phosphatidylinositol synthase; n=1; Neo... 53 5e-06
UniRef50_Q54P27 Cluster: Putative uncharacterized protein; n=1; ... 50 3e-05
UniRef50_Q10153 Cluster: Uncharacterized protein C1D4.08; n=2; A... 50 3e-05
UniRef50_P06197 Cluster: CDP-diacylglycerol--inositol 3-phosphat... 50 4e-05
UniRef50_Q8SX37 Cluster: RE35104p; n=4; Diptera|Rep: RE35104p - ... 48 1e-04
UniRef50_Q0CM69 Cluster: CDP-diacylglycerol--inositol 3-phosphat... 47 4e-04
UniRef50_Q9U2G1 Cluster: Putative uncharacterized protein pisy-1... 46 5e-04
UniRef50_A6QY05 Cluster: Putative uncharacterized protein; n=3; ... 45 0.002
UniRef50_A3FQQ0 Cluster: CDP-diacylglycerol--inositol 3-phosphat... 42 0.011
UniRef50_Q6KBS8 Cluster: Phosphatidylinositol synthase; n=1; Try... 41 0.020
UniRef50_UPI0000499628 Cluster: cdp-diacylglycerol--inositol 3-p... 41 0.026
UniRef50_Q5KCC1 Cluster: CDP-diacylglycerol-inositol 3-phosphati... 40 0.035
UniRef50_A7AU66 Cluster: Phosphatidylinositol synthase, putative... 36 0.75
UniRef50_Q8LBA6 Cluster: CDP-diacylglycerol--inositol 3-phosphat... 36 0.99
UniRef50_A3BBY4 Cluster: Putative uncharacterized protein; n=1; ... 35 1.3
UniRef50_Q2MJB9 Cluster: Phosphatidylinositol synthase; n=7; Pla... 34 3.0
UniRef50_A6RF53 Cluster: Predicted protein; n=1; Ajellomyces cap... 34 3.0
UniRef50_A1HTX3 Cluster: Putative uncharacterized protein; n=1; ... 33 5.3
UniRef50_Q5Q1M8 Cluster: Signal peptidase; n=4; Plasmodium (Vinc... 33 7.0
UniRef50_Q4JBI5 Cluster: Conserved Archaeal membrane protein; n=... 32 9.3
>UniRef50_Q4TI68 Cluster: Chromosome undetermined SCAF2366, whole
genome shotgun sequence; n=3; cellular organisms|Rep:
Chromosome undetermined SCAF2366, whole genome shotgun
sequence - Tetraodon nigroviridis (Green puffer)
Length = 285
Score = 64.9 bits (151), Expect = 1e-09
Identities = 28/46 (60%), Positives = 36/46 (78%)
Frame = +3
Query: 15 LQGKASHKFIDMSENPIMRAYYTNKWLLFYMCACNEAFYASLYVLH 152
++G +SHK ID+S NPI+R YYT+K +LF MCA NE F+ LYVLH
Sbjct: 192 VKGSSSHKSIDLSGNPILRIYYTSKPVLFVMCAGNELFFCLLYVLH 237
>UniRef50_O14735 Cluster: CDP-diacylglycerol--inositol
3-phosphatidyltransferase; n=22; Eumetazoa|Rep:
CDP-diacylglycerol--inositol 3-phosphatidyltransferase -
Homo sapiens (Human)
Length = 213
Score = 64.5 bits (150), Expect = 2e-09
Identities = 26/52 (50%), Positives = 37/52 (71%)
Frame = +3
Query: 9 AMLQGKASHKFIDMSENPIMRAYYTNKWLLFYMCACNEAFYASLYVLHFYSG 164
++++G SHK ID+S NP++R YYT++ LF +CA NE FY LY+ HF G
Sbjct: 112 SVVRGSESHKMIDLSGNPVLRIYYTSRPALFTLCAGNELFYCLLYLFHFSEG 163
>UniRef50_UPI000065FDB0 Cluster: CDP-diacylglycerol--inositol
3-phosphatidyltransferase (EC 2.7.8.11)
(Phosphatidylinositol synthase) (PtdIns synthase) (PI
synthase).; n=1; Takifugu rubripes|Rep:
CDP-diacylglycerol--inositol 3-phosphatidyltransferase
(EC 2.7.8.11) (Phosphatidylinositol synthase) (PtdIns
synthase) (PI synthase). - Takifugu rubripes
Length = 224
Score = 63.3 bits (147), Expect = 4e-09
Identities = 26/46 (56%), Positives = 36/46 (78%)
Frame = +3
Query: 15 LQGKASHKFIDMSENPIMRAYYTNKWLLFYMCACNEAFYASLYVLH 152
++G +SHK ID+S NPI+R YYT+K +LF MCA NE F+ LY+L+
Sbjct: 133 IKGSSSHKLIDLSGNPILRIYYTSKPVLFLMCAGNELFFCLLYILY 178
>UniRef50_Q6NYP5 Cluster: CDP-diacylglycerol--inositol
3-phosphatidyltransferase; n=6; Euteleostomi|Rep:
CDP-diacylglycerol--inositol 3-phosphatidyltransferase -
Danio rerio (Zebrafish) (Brachydanio rerio)
Length = 213
Score = 61.7 bits (143), Expect = 1e-08
Identities = 24/49 (48%), Positives = 37/49 (75%)
Frame = +3
Query: 9 AMLQGKASHKFIDMSENPIMRAYYTNKWLLFYMCACNEAFYASLYVLHF 155
+M++G SHK ID+S NPI+R YYT++ +LF+MC NE F+ LY++ +
Sbjct: 112 SMMKGATSHKAIDLSGNPILRLYYTSRPVLFFMCMGNELFFCLLYIMFY 160
>UniRef50_Q6BUR5 Cluster: Similar to sp|P06197 Saccharomyces
cerevisiae YPR113w PIS1; n=4; Saccharomycetales|Rep:
Similar to sp|P06197 Saccharomyces cerevisiae YPR113w
PIS1 - Debaryomyces hansenii (Yeast) (Torulaspora
hansenii)
Length = 225
Score = 55.2 bits (127), Expect = 1e-06
Identities = 23/49 (46%), Positives = 34/49 (69%)
Frame = +3
Query: 12 MLQGKASHKFIDMSENPIMRAYYTNKWLLFYMCACNEAFYASLYVLHFY 158
M G ASHK +D S++ I+ YYTN+ +LF +C NE FY ++Y LH++
Sbjct: 130 MSSGSASHKNVDESQSKILSLYYTNRTVLFVVCLVNELFYVAIY-LHYF 177
>UniRef50_A1DBH6 Cluster: Phosphatidylinositol synthase; n=1;
Neosartorya fischeri NRRL 181|Rep: Phosphatidylinositol
synthase - Neosartorya fischeri (strain ATCC 1020 / DSM
3700 / NRRL 181)(Aspergillus fischerianus (strain ATCC
1020 / DSM 3700 / NRRL 181))
Length = 234
Score = 53.2 bits (122), Expect = 5e-06
Identities = 30/64 (46%), Positives = 40/64 (62%)
Frame = +3
Query: 30 SHKFIDMSENPIMRAYYTNKWLLFYMCACNEAFYASLYVLHFYSGXTSMYKIYCNLSPNL 209
SHK ID S +MR YY+N+ +LF +C NEAF+ +LY+L F S SM ++ LS L
Sbjct: 139 SHKNIDESRPWVMRTYYSNQKVLFTVCTMNEAFFIALYLLAFSS--PSMPWLWLTLSSPL 196
Query: 210 PLTK 221
L K
Sbjct: 197 MLFK 200
>UniRef50_Q54P27 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 207
Score = 50.4 bits (115), Expect = 3e-05
Identities = 24/61 (39%), Positives = 35/61 (57%), Gaps = 1/61 (1%)
Frame = +3
Query: 9 AMLQ-GKASHKFIDMSENPIMRAYYTNKWLLFYMCACNEAFYASLYVLHFYSGXTSMYKI 185
A LQ G SHK + + IM+ YY NK+ L +MC NE F+ Y+ HFY+ + Y +
Sbjct: 111 ATLQSGSKSHKAVKENHIKIMKIYYGNKYFLAFMCFGNEGFFLFSYLYHFYATSSVFYIL 170
Query: 186 Y 188
+
Sbjct: 171 W 171
>UniRef50_Q10153 Cluster: Uncharacterized protein C1D4.08; n=2;
Ascomycota|Rep: Uncharacterized protein C1D4.08 -
Schizosaccharomyces pombe (Fission yeast)
Length = 251
Score = 50.4 bits (115), Expect = 3e-05
Identities = 21/46 (45%), Positives = 29/46 (63%)
Frame = +3
Query: 18 QGKASHKFIDMSENPIMRAYYTNKWLLFYMCACNEAFYASLYVLHF 155
QG +SHK + N ++R YY N +LF CA NE F+ +LY+L F
Sbjct: 134 QGASSHKTVTKKHNWMLRLYYGNNKVLFIFCAANEMFFVALYLLSF 179
>UniRef50_P06197 Cluster: CDP-diacylglycerol--inositol
3-phosphatidyltransferase; n=7; Saccharomycetales|Rep:
CDP-diacylglycerol--inositol 3-phosphatidyltransferase -
Saccharomyces cerevisiae (Baker's yeast)
Length = 220
Score = 50.0 bits (114), Expect = 4e-05
Identities = 22/50 (44%), Positives = 31/50 (62%)
Frame = +3
Query: 21 GKASHKFIDMSENPIMRAYYTNKWLLFYMCACNEAFYASLYVLHFYSGXT 170
GK SHK + E+ ++ YYT + +LF +CA NE FYA LY+ F + T
Sbjct: 125 GKTSHKSVGEGESRLLHLYYTRRDVLFTICAFNELFYAGLYLQLFSNSAT 174
>UniRef50_Q8SX37 Cluster: RE35104p; n=4; Diptera|Rep: RE35104p -
Drosophila melanogaster (Fruit fly)
Length = 224
Score = 48.4 bits (110), Expect = 1e-04
Identities = 28/68 (41%), Positives = 42/68 (61%), Gaps = 4/68 (5%)
Frame = +3
Query: 9 AMLQGKASHKFIDMSENPIMRAYYTNKWLLFYMCACNEAFYASLYVLHFYSG----XTSM 176
+++ G++SHK +++N IMR YY K +L +MC NE FY LY+LHF G S+
Sbjct: 115 SVVVGRSSHK---VNDNFIMRLYY-QKDILTFMCCVNELFYVCLYLLHFTYGPLIFGASL 170
Query: 177 YKIYCNLS 200
+KI L+
Sbjct: 171 FKILAFLT 178
>UniRef50_Q0CM69 Cluster: CDP-diacylglycerol--inositol
3-phosphatidyltransferase; n=14; Pezizomycotina|Rep:
CDP-diacylglycerol--inositol 3-phosphatidyltransferase -
Aspergillus terreus (strain NIH 2624)
Length = 300
Score = 46.8 bits (106), Expect = 4e-04
Identities = 22/44 (50%), Positives = 29/44 (65%)
Frame = +3
Query: 30 SHKFIDMSENPIMRAYYTNKWLLFYMCACNEAFYASLYVLHFYS 161
SHK I+ S + I+ YY +K +LF CA NEAF+ LY+L F S
Sbjct: 164 SHKKIEASRSWILYQYYHSKTVLFICCALNEAFFIGLYLLSFSS 207
>UniRef50_Q9U2G1 Cluster: Putative uncharacterized protein pisy-1;
n=2; Caenorhabditis|Rep: Putative uncharacterized
protein pisy-1 - Caenorhabditis elegans
Length = 220
Score = 46.4 bits (105), Expect = 5e-04
Identities = 22/50 (44%), Positives = 30/50 (60%)
Frame = +3
Query: 15 LQGKASHKFIDMSENPIMRAYYTNKWLLFYMCACNEAFYASLYVLHFYSG 164
L G SHK S N ++ YYT++ L +MC NEAFY LY+ F++G
Sbjct: 120 LSGATSHK---QSSNWLLNLYYTDRLFLGFMCGGNEAFYILLYIRAFWAG 166
>UniRef50_A6QY05 Cluster: Putative uncharacterized protein; n=3;
Pezizomycotina|Rep: Putative uncharacterized protein -
Ajellomyces capsulatus NAm1
Length = 337
Score = 44.8 bits (101), Expect = 0.002
Identities = 20/44 (45%), Positives = 27/44 (61%)
Frame = +3
Query: 30 SHKFIDMSENPIMRAYYTNKWLLFYMCACNEAFYASLYVLHFYS 161
SHK +D S + I+ YY +K +LF C NE F+ LY+L F S
Sbjct: 185 SHKKVDSSRSWILYQYYNSKTVLFVFCLFNELFFIGLYLLSFSS 228
>UniRef50_A3FQQ0 Cluster: CDP-diacylglycerol--inositol
3-phosphatidyltransferase isoform 1;
phosphatidylinositol synthase; PtdIns synthase; PI
synthase; n=2; Cryptosporidium|Rep:
CDP-diacylglycerol--inositol 3-phosphatidyltransferase
isoform 1; phosphatidylinositol synthase; PtdIns
synthase; PI synthase - Cryptosporidium parvum Iowa II
Length = 154
Score = 41.9 bits (94), Expect = 0.011
Identities = 20/41 (48%), Positives = 26/41 (63%)
Frame = +3
Query: 21 GKASHKFIDMSENPIMRAYYTNKWLLFYMCACNEAFYASLY 143
G +SHK I PI++ YY+ K LLF + ACNEA + LY
Sbjct: 48 GGSSHKSIKEEMWPILKLYYSKKPLLFTLHACNEALWLILY 88
>UniRef50_Q6KBS8 Cluster: Phosphatidylinositol synthase; n=1;
Trypanosoma brucei|Rep: Phosphatidylinositol synthase -
Trypanosoma brucei
Length = 217
Score = 41.1 bits (92), Expect = 0.020
Identities = 21/75 (28%), Positives = 35/75 (46%)
Frame = +3
Query: 9 AMLQGKASHKFIDMSENPIMRAYYTNKWLLFYMCACNEAFYASLYVLHFYSGXTSMYKIY 188
++ G+ SHK + S ++R YYTN+ + +C E FY +LY+ Y + +
Sbjct: 122 SLYAGRTSHKDVSSSIFSLLRLYYTNRPFMCALCVGQELFYLNLYMYGVYGIAEPAFLFF 181
Query: 189 CNLSPNLPLTKFCCN 233
L+ L K N
Sbjct: 182 MALTAALSAFKQVVN 196
>UniRef50_UPI0000499628 Cluster: cdp-diacylglycerol--inositol
3-phosphatidyltransferase, putative; n=1; Entamoeba
histolytica HM-1:IMSS|Rep: cdp-diacylglycerol--inositol
3-phosphatidyltransferase, putative - Entamoeba
histolytica HM-1:IMSS
Length = 194
Score = 40.7 bits (91), Expect = 0.026
Identities = 22/58 (37%), Positives = 33/58 (56%)
Frame = +3
Query: 9 AMLQGKASHKFIDMSENPIMRAYYTNKWLLFYMCACNEAFYASLYVLHFYSGXTSMYK 182
++++G SHK + NPI+ YYT +LF +C CNEA + Y+ F T+ YK
Sbjct: 90 SLIKGGESHK---ICSNPILHWYYTRS-VLFTLCLCNEATFVMWYIRFF--AQTNEYK 141
>UniRef50_Q5KCC1 Cluster: CDP-diacylglycerol-inositol
3-phosphatidyltransferase, putative; n=3;
Basidiomycota|Rep: CDP-diacylglycerol-inositol
3-phosphatidyltransferase, putative - Cryptococcus
neoformans (Filobasidiella neoformans)
Length = 354
Score = 40.3 bits (90), Expect = 0.035
Identities = 16/51 (31%), Positives = 28/51 (54%)
Frame = +3
Query: 9 AMLQGKASHKFIDMSENPIMRAYYTNKWLLFYMCACNEAFYASLYVLHFYS 161
++ G +SHK + + I+ YY + LF C NE F+ LY+ H+++
Sbjct: 213 SLTTGSSSHKTVTSDVSRILWYYYNDSRTLFVFCFANELFFVCLYLNHYWT 263
>UniRef50_A7AU66 Cluster: Phosphatidylinositol synthase, putative;
n=1; Babesia bovis|Rep: Phosphatidylinositol synthase,
putative - Babesia bovis
Length = 208
Score = 35.9 bits (79), Expect = 0.75
Identities = 16/55 (29%), Positives = 25/55 (45%)
Frame = +3
Query: 15 LQGKASHKFIDMSENPIMRAYYTNKWLLFYMCACNEAFYASLYVLHFYSGXTSMY 179
L +HK + N +R YY +WL+F E F+ +LY+ Y +Y
Sbjct: 114 LYSNTNHKDVK-DANIFLRVYYHKRWLMFMSIVMYETFFCTLYIRSMYHPGDYIY 167
>UniRef50_Q8LBA6 Cluster: CDP-diacylglycerol--inositol
3-phosphatidyltransferase 1; n=9; Magnoliophyta|Rep:
CDP-diacylglycerol--inositol 3-phosphatidyltransferase 1
- Arabidopsis thaliana (Mouse-ear cress)
Length = 227
Score = 35.5 bits (78), Expect = 0.99
Identities = 19/68 (27%), Positives = 31/68 (45%)
Frame = +3
Query: 15 LQGKASHKFIDMSENPIMRAYYTNKWLLFYMCACNEAFYASLYVLHFYSGXTSMYKIYCN 194
L GK+SHK + S + + R YY N+ + Y C E Y L ++ + +
Sbjct: 121 LAGKSSHKDVKDSTSWLFRLYYGNRIFMCYCCVSCEVLYIILLLIAKNQSENLLNVVVAT 180
Query: 195 LSPNLPLT 218
L+ PL+
Sbjct: 181 LTQISPLS 188
>UniRef50_A3BBY4 Cluster: Putative uncharacterized protein; n=1;
Oryza sativa (japonica cultivar-group)|Rep: Putative
uncharacterized protein - Oryza sativa subsp. japonica
(Rice)
Length = 190
Score = 35.1 bits (77), Expect = 1.3
Identities = 19/70 (27%), Positives = 34/70 (48%)
Frame = +3
Query: 15 LQGKASHKFIDMSENPIMRAYYTNKWLLFYMCACNEAFYASLYVLHFYSGXTSMYKIYCN 194
L GK SHK + + N +++ YY ++ + + C +E Y L++ + + C
Sbjct: 81 LSGKTSHKDVKDTGNWLLKLYYGHRPFMAFCCVASEVLYIILFLFADEKSTSLLNVCRCF 140
Query: 195 LSPNLPLTKF 224
L + PLT F
Sbjct: 141 LKGS-PLTFF 149
>UniRef50_Q2MJB9 Cluster: Phosphatidylinositol synthase; n=7;
Plasmodium|Rep: Phosphatidylinositol synthase -
Plasmodium falciparum
Length = 209
Score = 33.9 bits (74), Expect = 3.0
Identities = 19/71 (26%), Positives = 33/71 (46%), Gaps = 1/71 (1%)
Frame = +3
Query: 15 LQGKASHKFIDMSENPIMRAYYTNKWLLFYMCACNEAFYASLYVLHFYSGXTSMYKI-YC 191
+ G +HK I+ N +++ YY W++ E+F Y+L + +YK+ Y
Sbjct: 116 IAGNKTHKKIEKG-NKLLKLYYEKPWVMVICIIAYESFLICAYLLRVAVKKSLIYKLSYY 174
Query: 192 NLSPNLPLTKF 224
L + PL F
Sbjct: 175 ALICSFPLAAF 185
>UniRef50_A6RF53 Cluster: Predicted protein; n=1; Ajellomyces
capsulatus NAm1|Rep: Predicted protein - Ajellomyces
capsulatus NAm1
Length = 330
Score = 33.9 bits (74), Expect = 3.0
Identities = 22/64 (34%), Positives = 31/64 (48%), Gaps = 5/64 (7%)
Frame = +1
Query: 1 RHEQCSRARL---LINSLTCLRILSCVPIT-PTNGSFSICVLAMKPSTHRCTFCTSI-QD 165
R QC R + L + + L+ + +T PT S VLA KP T+RC + SI
Sbjct: 171 RSSQCKRTNMQPRLFKAAALVLFLTPLALTAPTENDASADVLACKPGTYRCAYQDSITSS 230
Query: 166 XQVC 177
+VC
Sbjct: 231 IEVC 234
>UniRef50_A1HTX3 Cluster: Putative uncharacterized protein; n=1;
Thermosinus carboxydivorans Nor1|Rep: Putative
uncharacterized protein - Thermosinus carboxydivorans
Nor1
Length = 325
Score = 33.1 bits (72), Expect = 5.3
Identities = 15/40 (37%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = +1
Query: 88 NGSFSICVLAMKPSTHRCTFCTSIQDXQVC-IKYTAICLL 204
N + C+LA T+ C FC+ +Q +VC +T IC+L
Sbjct: 12 NSDYCPCLLA---ETNHCVFCSHLQGKEVCDCNWTGICIL 48
>UniRef50_Q5Q1M8 Cluster: Signal peptidase; n=4; Plasmodium
(Vinckeia)|Rep: Signal peptidase - Plasmodium yoelii
Length = 346
Score = 32.7 bits (71), Expect = 7.0
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = +3
Query: 84 NKWLLFYMCACNEAFYASLYVLHFYSGXTSMYKIYCNLSPNLPLTKF 224
N +LFY+C C+ F+ L + Y+ +YKIY + NL F
Sbjct: 170 NGVILFYICDCSLRFFNELRNIAIYNYINILYKIYNIIHRNLDNINF 216
>UniRef50_Q4JBI5 Cluster: Conserved Archaeal membrane protein; n=3;
Sulfolobaceae|Rep: Conserved Archaeal membrane protein -
Sulfolobus acidocaldarius
Length = 466
Score = 32.3 bits (70), Expect = 9.3
Identities = 17/52 (32%), Positives = 25/52 (48%)
Frame = +2
Query: 359 SQAGTNFSNEIRTQQMFTIDFHGEGTASYNNNETRKIIICVITGGRTSCAVG 514
S GTNFS+ + + G G A+Y ET+K + TG + + VG
Sbjct: 187 SPVGTNFSSLGTAMVLTIVSIAGAGAAAYLGEETKKPTSTITTGIKIAYVVG 238
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 595,859,845
Number of Sequences: 1657284
Number of extensions: 11674836
Number of successful extensions: 25940
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 25196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25934
length of database: 575,637,011
effective HSP length: 97
effective length of database: 414,880,463
effective search space used: 43562448615
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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